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The map label for this gene is rpoA

Identifier: 108758094

GI number: 108758094

Start: 3869058

End: 3870080

Strand: Direct

Name: rpoA

Synonym: MXAN_3326

Alternate gene names: 108758094

Gene position: 3869058-3870080 (Clockwise)

Preceding gene: 108758664

Following gene: 108762497

Centisome position: 42.33

GC content: 64.03

Gene sequence:

>1023_bases
ATGGCTGATACGTTCGTTGCGAAGAACTGGCGTGACCTGATCAAGCCCCGTCGCATGGAGGTGGATCAGGACAGCGCCAC
CCCCACTTACGGTAAGTTCGTCGCGGAGCCGCTCGAGCGCGGTTTCGGTACGACGCTGGGCAACTCGCTCCGCCGGGTGC
TGCTGTCGTCGCTGCAGGGCGCCGCCATCACCACCGTCAAGATTGAAGGCGTGGACCATGAGTTCACGACCATCCCCGAG
GTGTCCGAGGACGTCACGGACGTCGTGCTGAACCTGAAGGAAGTCCTCCTTCGGATGCACACGAACGAGACGAAGACGCT
CCGCATCGAGGCGGAGGGCCCCAAGGAGGTCAAGGCCGGTGACATCATCACCGACCCGGACACCGAGATCCTCAACCCGG
GCCACCACATCTGCACCATCTCCGAGGGCGGCAAGCTCCGCATGGAGCTGACCTGCCGCCGCGGCCGTGGTTACACGCCG
GCCAACGTGAACAAGGTCGCCGGTTCGCCCATCGGCACCATCCCCATCGACTCGCTGTTCTCGCCCATCCGCAAGGTGAA
CTACCAGGTCACCAACGCGCGCGTCGGTCAGGTCACGGACTTCGACAAGCTGTCGCTCGAGGTCTGGACGGACGGCTCCG
TGTCTCCACAGGACGCGGTGGCGTACGCGGCGAAGATCATCAAGGAGCAGCTCACCGTCTTCGTGAACTTCGACGAGACG
GAGGAGCCCGTCGTCGCCGAGGCGCCGAAGGAAGAGGCGAAGCTGAACGAGAACCTGTTCCGCTCGGTGGATGAGCTCGA
GCTGTCGGTCCGCTCGGCGAACTGCCTGCAGCAGGCGAACATCAAGTCCATTGGCGACCTCGTGCAGCGCACCGAGGCCG
AGATGCTCAAGACGAAGAACTTCGGCCGCAAGTCTCTGAAGGAGATCAAGGAGATCCTCGCGGAGATGGGCTTGTCGCTG
GGCATGAAGCTGGAGAACTGGCCGCCGAAGCAGGCGCCGGCTCCCGCGCAGCCGAAGGCGTAG

Upstream 100 bases:

>100_bases
GGCGTCTTGCTTTCAAGCAGTCGCAGTCCCCGCGTGCGCATCCTCCCGCCAGGCCGGTGGGTAAGTCGGTGGTGCGGCAC
GAACCGAGGAGCGGTACACC

Downstream 100 bases:

>100_bases
TAGGGTTCACGCAGGGCCTCCCCACGTGGCCGCGGTACGGCGGTCAGCGGCGCGAGGGGAGGGGGCTGGGACGTCTTCCG
GTCCGAATGACAGTGGCGGG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MADTFVAKNWRDLIKPRRMEVDQDSATPTYGKFVAEPLERGFGTTLGNSLRRVLLSSLQGAAITTVKIEGVDHEFTTIPE
VSEDVTDVVLNLKEVLLRMHTNETKTLRIEAEGPKEVKAGDIITDPDTEILNPGHHICTISEGGKLRMELTCRRGRGYTP
ANVNKVAGSPIGTIPIDSLFSPIRKVNYQVTNARVGQVTDFDKLSLEVWTDGSVSPQDAVAYAAKIIKEQLTVFVNFDET
EEPVVAEAPKEEAKLNENLFRSVDELELSVRSANCLQQANIKSIGDLVQRTEAEMLKTKNFGRKSLKEIKEILAEMGLSL
GMKLENWPPKQAPAPAQPKA

Sequences:

>Translated_340_residues
MADTFVAKNWRDLIKPRRMEVDQDSATPTYGKFVAEPLERGFGTTLGNSLRRVLLSSLQGAAITTVKIEGVDHEFTTIPE
VSEDVTDVVLNLKEVLLRMHTNETKTLRIEAEGPKEVKAGDIITDPDTEILNPGHHICTISEGGKLRMELTCRRGRGYTP
ANVNKVAGSPIGTIPIDSLFSPIRKVNYQVTNARVGQVTDFDKLSLEVWTDGSVSPQDAVAYAAKIIKEQLTVFVNFDET
EEPVVAEAPKEEAKLNENLFRSVDELELSVRSANCLQQANIKSIGDLVQRTEAEMLKTKNFGRKSLKEIKEILAEMGLSL
GMKLENWPPKQAPAPAQPKA
>Mature_339_residues
ADTFVAKNWRDLIKPRRMEVDQDSATPTYGKFVAEPLERGFGTTLGNSLRRVLLSSLQGAAITTVKIEGVDHEFTTIPEV
SEDVTDVVLNLKEVLLRMHTNETKTLRIEAEGPKEVKAGDIITDPDTEILNPGHHICTISEGGKLRMELTCRRGRGYTPA
NVNKVAGSPIGTIPIDSLFSPIRKVNYQVTNARVGQVTDFDKLSLEVWTDGSVSPQDAVAYAAKIIKEQLTVFVNFDETE
EPVVAEAPKEEAKLNENLFRSVDELELSVRSANCLQQANIKSIGDLVQRTEAEMLKTKNFGRKSLKEIKEILAEMGLSLG
MKLENWPPKQAPAPAQPKA

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=322, Percent_Identity=46.8944099378882, Blast_Score=291, Evalue=3e-80,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_MYXXD (Q1D748)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_631525.1
- ProteinModelPortal:   Q1D748
- STRING:   Q1D748
- GeneID:   4101738
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_3326
- TIGR:   MXAN_3326
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- PhylomeDB:   Q1D748
- ProtClustDB:   PRK05182
- BioCyc:   MXAN246197:MXAN_3326-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37566; Mature: 37435

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTFVAKNWRDLIKPRRMEVDQDSATPTYGKFVAEPLERGFGTTLGNSLRRVLLSSLQG
CCCCHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
AAITTVKIEGVDHEFTTIPEVSEDVTDVVLNLKEVLLRMHTNETKTLRIEAEGPKEVKAG
CEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
DIITDPDTEILNPGHHICTISEGGKLRMELTCRRGRGYTPANVNKVAGSPIGTIPIDSLF
CCCCCCCHHHCCCCCCEEEECCCCEEEEEEEEECCCCCCCCCCHHHCCCCCCCCCHHHHH
SPIRKVNYQVTNARVGQVTDFDKLSLEVWTDGSVSPQDAVAYAAKIIKEQLTVFVNFDET
HHHHHCCEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEECCCC
EEPVVAEAPKEEAKLNENLFRSVDELELSVRSANCLQQANIKSIGDLVQRTEAEMLKTKN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
FGRKSLKEIKEILAEMGLSLGMKLENWPPKQAPAPAQPKA
CCHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADTFVAKNWRDLIKPRRMEVDQDSATPTYGKFVAEPLERGFGTTLGNSLRRVLLSSLQG
CCCHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
AAITTVKIEGVDHEFTTIPEVSEDVTDVVLNLKEVLLRMHTNETKTLRIEAEGPKEVKAG
CEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
DIITDPDTEILNPGHHICTISEGGKLRMELTCRRGRGYTPANVNKVAGSPIGTIPIDSLF
CCCCCCCHHHCCCCCCEEEECCCCEEEEEEEEECCCCCCCCCCHHHCCCCCCCCCHHHHH
SPIRKVNYQVTNARVGQVTDFDKLSLEVWTDGSVSPQDAVAYAAKIIKEQLTVFVNFDET
HHHHHCCEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEECCCC
EEPVVAEAPKEEAKLNENLFRSVDELELSVRSANCLQQANIKSIGDLVQRTEAEMLKTKN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
FGRKSLKEIKEILAEMGLSLGMKLENWPPKQAPAPAQPKA
CCHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA