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The map label for this gene is nolF [H]

Identifier: 108758003

GI number: 108758003

Start: 595982

End: 597247

Strand: Direct

Name: nolF [H]

Synonym: MXAN_0527

Alternate gene names: 108758003

Gene position: 595982-597247 (Clockwise)

Preceding gene: 108763421

Following gene: 108757248

Centisome position: 6.52

GC content: 72.99

Gene sequence:

>1266_bases
GTGAGACGGGCTCGAATACGACAGGTCTTCGGCGGAACCTCGGCGGGGATTCTGATGGGGCTCTGCGCCCTGTCCGGTTG
CCGGAAGGGTGAGGACGCAGCGCCCGCGCCTCGACAAGAGGAGCTGGAGATGACGCTGGGCCAGGAGAACGTGGCCCGCG
CCGAGGTGCGGCAGTTGCGCTCCGGTCCTGGCATCTCCGGCAACCTGCAGGCGCGCACGGCGGCGGCGGTGCGCGCGGAA
GTGGGCGGCACCATCCTCGACATCAAGGCCCAGCAGGGCCAGGTGGTGAAGAAGGGCGAGGAGCTGGCGCGCATCGAGGA
CGTCACGCTGAAGGACCAGCTCATCGCGGCGTCGGCGGCGGTCCGCACGGCCCGAAGCGCGCTCCAGGTAGCCGAGGCCG
AGCAGGAGCGCGCCACGAGGCTGTCCAAGGCGGGCGTCATCACCCAGCGCGACTTCGAACGGGCGGAGCTCTCCGTGGAG
CAGGCCAAGGGGCAGCTCGCGGAGGCGCGCTCCCGGCATGCCCTGGCGCAGGAGCAGCTCAACCGCGCGCGCGTCGTGGC
GCCCTTCGCGGGCGTGGTGAGCGAGCGGCAGGCCAGCGCGGGTGACGTCGTCCAGCCCAGCGCGCCGCTCTTCACGGTGG
TGGACCCGCGCACGTTGCGCCTGGAGGCCTCCGTGCCCGCCGCGCAGCTCGAACAGGTGAAGGTGGACACGCCGGTGGAG
TTCCACGTGACGGGCTATGGGGACCGCGCCTTCCGCGGCACGGTGGAGCGCATCAACCCGGTGGTGGACCCGGCCACCGG
CCAGGTCCGCATCTACGTGGCCATTCCCAACACGGACCTCCAATTGCTGGCCGGCCTCTTCGCGGAGGGGCGCGTGGCCT
CGAAGGGTACGCAGGCGGTCGCGATTCCCATCGACGCCATCGACGACACGGGGACCGAGCCCGCCGTGCTGCGCATCCAG
GAAGGGCGCGCGCAGCGGGTGAACGTCAGATTGGGCCTGCGCGACGAGGTGGCGCGGCAGGTCGAGGTCCGCTCCGGTCT
GGAGGACGGCGACGTGGTGCTGCTGGGCGCCGCGCGGAGCGAGGTGGCGGAGGGCGGGCGCGTGAAGGTGTCGTCCCCAC
GGCCGGACTCGAAGCCCGTGGAGGAGGCGGGGCCCGGTGTTGGCGGCTCGGAGGCGCCGCCGCCCCCGTCGTCGGGAGCG
AGGCAGCCCACTCCGCGGCCCGCGTCCACGCAACCCCCGGCGGAGGACGCTGCGCCGTCCCACTAA

Upstream 100 bases:

>100_bases
CTTGCTGGCGGTGGGCCTTGTTGCCCTCGGCTACCGGGGGCAGGGGGCCGACACCATGGTTAGCCGGCTCGCCGTGGGGG
GCGGGGATGGGAGACGCGCT

Downstream 100 bases:

>100_bases
TGCTCCAAGCCTGGAGTCACGTCCGTGTTCATCTCATACTTCGCCATCAAGCGGCCCATCATCACCATCAATTCGATGCT
GGCGCTGGTGGTCTTTGGCC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MRRARIRQVFGGTSAGILMGLCALSGCRKGEDAAPAPRQEELEMTLGQENVARAEVRQLRSGPGISGNLQARTAAAVRAE
VGGTILDIKAQQGQVVKKGEELARIEDVTLKDQLIAASAAVRTARSALQVAEAEQERATRLSKAGVITQRDFERAELSVE
QAKGQLAEARSRHALAQEQLNRARVVAPFAGVVSERQASAGDVVQPSAPLFTVVDPRTLRLEASVPAAQLEQVKVDTPVE
FHVTGYGDRAFRGTVERINPVVDPATGQVRIYVAIPNTDLQLLAGLFAEGRVASKGTQAVAIPIDAIDDTGTEPAVLRIQ
EGRAQRVNVRLGLRDEVARQVEVRSGLEDGDVVLLGAARSEVAEGGRVKVSSPRPDSKPVEEAGPGVGGSEAPPPPSSGA
RQPTPRPASTQPPAEDAAPSH

Sequences:

>Translated_421_residues
MRRARIRQVFGGTSAGILMGLCALSGCRKGEDAAPAPRQEELEMTLGQENVARAEVRQLRSGPGISGNLQARTAAAVRAE
VGGTILDIKAQQGQVVKKGEELARIEDVTLKDQLIAASAAVRTARSALQVAEAEQERATRLSKAGVITQRDFERAELSVE
QAKGQLAEARSRHALAQEQLNRARVVAPFAGVVSERQASAGDVVQPSAPLFTVVDPRTLRLEASVPAAQLEQVKVDTPVE
FHVTGYGDRAFRGTVERINPVVDPATGQVRIYVAIPNTDLQLLAGLFAEGRVASKGTQAVAIPIDAIDDTGTEPAVLRIQ
EGRAQRVNVRLGLRDEVARQVEVRSGLEDGDVVLLGAARSEVAEGGRVKVSSPRPDSKPVEEAGPGVGGSEAPPPPSSGA
RQPTPRPASTQPPAEDAAPSH
>Mature_421_residues
MRRARIRQVFGGTSAGILMGLCALSGCRKGEDAAPAPRQEELEMTLGQENVARAEVRQLRSGPGISGNLQARTAAAVRAE
VGGTILDIKAQQGQVVKKGEELARIEDVTLKDQLIAASAAVRTARSALQVAEAEQERATRLSKAGVITQRDFERAELSVE
QAKGQLAEARSRHALAQEQLNRARVVAPFAGVVSERQASAGDVVQPSAPLFTVVDPRTLRLEASVPAAQLEQVKVDTPVE
FHVTGYGDRAFRGTVERINPVVDPATGQVRIYVAIPNTDLQLLAGLFAEGRVASKGTQAVAIPIDAIDDTGTEPAVLRIQ
EGRAQRVNVRLGLRDEVARQVEVRSGLEDGDVVLLGAARSEVAEGGRVKVSSPRPDSKPVEEAGPGVGGSEAPPPPSSGA
RQPTPRPASTQPPAEDAAPSH

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Attached To The Inner Membrane By A Lipid Anchor [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=312, Percent_Identity=26.6025641025641, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082036, Length=329, Percent_Identity=25.8358662613982, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081796, Length=318, Percent_Identity=25.7861635220126, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789665, Length=376, Percent_Identity=23.4042553191489, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44407; Mature: 44407

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRARIRQVFGGTSAGILMGLCALSGCRKGEDAAPAPRQEELEMTLGQENVARAEVRQLR
CCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHH
SGPGISGNLQARTAAAVRAEVGGTILDIKAQQGQVVKKGEELARIEDVTLKDQLIAASAA
CCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHCCHHHHHHHCCCHHHHHHHHHHH
VRTARSALQVAEAEQERATRLSKAGVITQRDFERAELSVEQAKGQLAEARSRHALAQEQL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRARVVAPFAGVVSERQASAGDVVQPSAPLFTVVDPRTLRLEASVPAAQLEQVKVDTPVE
HHHEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEEECCCCHHHHHHEECCCCEE
FHVTGYGDRAFRGTVERINPVVDPATGQVRIYVAIPNTDLQLLAGLFAEGRVASKGTQAV
EEEECCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCEEE
AIPIDAIDDTGTEPAVLRIQEGRAQRVNVRLGLRDEVARQVEVRSGLEDGDVVLLGAARS
EEEEECCCCCCCCCEEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCCCCEEEEECCHH
EVAEGGRVKVSSPRPDSKPVEEAGPGVGGSEAPPPPSSGARQPTPRPASTQPPAEDAAPS
HHCCCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
H
C
>Mature Secondary Structure
MRRARIRQVFGGTSAGILMGLCALSGCRKGEDAAPAPRQEELEMTLGQENVARAEVRQLR
CCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHH
SGPGISGNLQARTAAAVRAEVGGTILDIKAQQGQVVKKGEELARIEDVTLKDQLIAASAA
CCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHCCHHHHHHHCCCHHHHHHHHHHH
VRTARSALQVAEAEQERATRLSKAGVITQRDFERAELSVEQAKGQLAEARSRHALAQEQL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRARVVAPFAGVVSERQASAGDVVQPSAPLFTVVDPRTLRLEASVPAAQLEQVKVDTPVE
HHHEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEEECCCCHHHHHHEECCCCEE
FHVTGYGDRAFRGTVERINPVVDPATGQVRIYVAIPNTDLQLLAGLFAEGRVASKGTQAV
EEEECCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCEEE
AIPIDAIDDTGTEPAVLRIQEGRAQRVNVRLGLRDEVARQVEVRSGLEDGDVVLLGAARS
EEEEECCCCCCCCCEEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCCCCEEEEECCHH
EVAEGGRVKVSSPRPDSKPVEEAGPGVGGSEAPPPPSSGARQPTPRPASTQPPAEDAAPS
HHCCCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
H
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]