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The map label for this gene is pcrA [H]

Identifier: 108757579

GI number: 108757579

Start: 1777638

End: 1779989

Strand: Direct

Name: pcrA [H]

Synonym: MXAN_1516

Alternate gene names: 108757579

Gene position: 1777638-1779989 (Clockwise)

Preceding gene: 108757351

Following gene: 108756852

Centisome position: 19.45

GC content: 68.88

Gene sequence:

>2352_bases
ATGTCTCCCGCGAACCCACACGAATCCGCCCTCCTCGAAGACCTCAATGCGCCGCAGCGCGAGGCCGTGTTGCATGGTGA
CGGCCCCCTGCTCGTCCTGTCAGGGGCGGGCAGCGGCAAGACGCGCGTCATCACCCGGCGCGTGGCGTATCTGGTGAAGG
TCCACAACGTCTACCCGTGGCGCATCCTGGCCGTCACCTTCACCAACAAGGCGGCGCGGGAGATGCGCGAGCGCCTGGTG
CAGTTGCTGGGGGCCCAGGCGAACGAGCTGGTGGTGAGCACCTTCCACTCGGCGGCGGCGCAGATTCTCCGCCGAGAGGC
GGAGCACGTGGGGCTCACCCGCTCCTTCGTCATCTACGACGACTCGGACCAGCTCAACGTGGTGAAGCGCGCCATGCGCG
AGGCGGGCATCGAGTCCATGCAGCCGCGCGAAATCCTCCACCGCATCGACCAGGAGAAGAACGCGGCCCGGCTCCCGGAG
CACATGCAGGTCGCGCCCGGCGACGAGCGGGGCCAACTGGTGCGCAAGGTGTACGCGGCCTACCAGGAGCGGCTGCGCGC
GGCCAACGCGGTGGACTTCGGAGACCTGCTGCTCCTGCTGGTGACGCTGTTCCGCACCCGGCCGGACGTGCTGGAGAACT
ATCGGCGCCGCTTCCACCACGTGCTGGTGGATGAGTTCCAGGACACCAACCCGGTGCAGTACGCGCTGCTGAAGCAACTG
GCGCCGCCCCCTTCCGCCAACCTGGTGGTGGTGGGCGACGACGACCAGTCCATCTACCGGTGGCGTGGCGCGGACGTGGA
CAACATCCTCGGCTTTCCGCGGCAGTACCCCGGCGCGAAGGTGGTCAAGCTGGAGCAGAACTACCGCTCCGACCAGAACA
TCCTGGACGCGGCGCATGAGGTCATCCGGAAGAACACGCGGCGCATGGAGAAGAAGCTCTGGTCCGAGCGCCAGCGGGGC
CAGACGCTCAACCTGATGCTCAACCGCGATGAGCGGGCCGAGGGGCAGGAGGTGGCGCGGCAGATTCTGGCGCTGCAGCG
GGAGGGCTTCATCAAGCTCTCCAGCATGGCGGTGTTCTACCGGGCCAATGCGCAGAGCCGCGTGTTGGAAGAGGCGCTGC
GTCTGGCGCGCGTCCCGTACACCCTGGTGAGCGGACGCAGCTTCTATGACCGGGCGGAAGTGCGGGATGCCTCCGCGTAT
CTGCGGCTGATGGTGAACCCGCGCTCGGACGCGGACCTGCTGCGCATCATCAACATCCCGGCGCGTGGCATTGGCGACAC
CACGGTGGAGCGGGTGACGGACTTCGCCAACGCGCGCGGCGTGAGCCTCTACGAGGTCTTGTCCCAGCCGGAGCAGATTC
CCGCGCTCAATGGTGCCGCGGTGAAGCGGCTCAAGGGCTTCCGCAACCTGCTGGAGTCCCTGACGGCCTTCGCCCAGACG
AACGAGGAAGCCGCGGGCGCGGTGGACCAGATGCTTCGCGAGACGAAGCTGGTGGAGACGCTTCAGGCCGAGGGCAGCGA
CGAGTCTCAGACGCGCGCGGAGAACCTCCGCGAGTTCCTGGGCGCGGCGCAGGAGTTCGACCTGAACCGGGCGGCCGCGG
CGGTGGCGGCCGCGGCGAATCCCCCCAGCGACGTTCCTCCCGAAGTGGACGCGGCCCCGCTGACGGCGGACGTGCCGGCG
CTGCAGGCCTTCCTGGAGCAGATTTCGCTGGTGGGCGAGGCGGACGCGGAGGTGGGCGACGGCCGGGTGGCGCTGATGAC
GCTGCACGCGGCCAAGGGCCTGGAGTTCGACGCCGTCTTCCTCACCGGCATGGAGGAGAACGTCTTCCCGCACTCGCGCG
CGGTGAAGTCCGAGTCCACGTTCCTGAACGAGGTCGCCTCGGCGGATGGGGAGCCCTCGGAGGAGATGGCCGAGGAGCGG
CGCCTCTGCTACGTGGGTTTCACGCGCGCGCGAAAGCGGCTCTTCGTGAGTCTGGCCCAGTGTCGCTCGCTGTTCGGTGA
GCTGCGCTACAACGCGCCCAGCCGCTTCCTGCGGGATGTGCCGCCGGCGCTGTTCGGCATCAGCGAGCAGGAAGTCCCCG
AAGCGCCTCGGCCCATGGTGCCCTCCACGCAGCGCAGGCGGACCTGGGACGAGGATGACGGGCCGCGCATCGACCGCTCG
TACTCGCAGGCGTCGTCGGACATGGACGGCGTGGGCGGGGACGTGCGCGGCATGCGCGTGCGCCACGAGCAGTTCGGCGT
GGGCCGCGTGGTGTCCGCGGACGGCAACGGCCCCAACGCGAAGGTGACGGTGGAGTTCGGTGGAGGCGTGGGCCTCAAAC
GCATCATCGCCCGTTTCCTGATACCGGGCTGA

Upstream 100 bases:

>100_bases
TAAGCGCACCGCATGCCTTGGGGGCCGAGCGGCCGGGCGGGACTGCGGAAGGGCGCCCATTGGTGGGGGAAAGACGTTAG
AAGCATGGGTTCCCTCCCGA

Downstream 100 bases:

>100_bases
GAAGCGGGCACCCGAGTGGACAAGCCGGTTTGGAGACTTGTGGGAGCGACAAAAGAAAGCCCGCGCGCCTGACAGGGCGA
ATGCGCTGGTGGCAGAGTCC

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 783; Mature: 782

Protein sequence:

>783_residues
MSPANPHESALLEDLNAPQREAVLHGDGPLLVLSGAGSGKTRVITRRVAYLVKVHNVYPWRILAVTFTNKAAREMRERLV
QLLGAQANELVVSTFHSAAAQILRREAEHVGLTRSFVIYDDSDQLNVVKRAMREAGIESMQPREILHRIDQEKNAARLPE
HMQVAPGDERGQLVRKVYAAYQERLRAANAVDFGDLLLLLVTLFRTRPDVLENYRRRFHHVLVDEFQDTNPVQYALLKQL
APPPSANLVVVGDDDQSIYRWRGADVDNILGFPRQYPGAKVVKLEQNYRSDQNILDAAHEVIRKNTRRMEKKLWSERQRG
QTLNLMLNRDERAEGQEVARQILALQREGFIKLSSMAVFYRANAQSRVLEEALRLARVPYTLVSGRSFYDRAEVRDASAY
LRLMVNPRSDADLLRIINIPARGIGDTTVERVTDFANARGVSLYEVLSQPEQIPALNGAAVKRLKGFRNLLESLTAFAQT
NEEAAGAVDQMLRETKLVETLQAEGSDESQTRAENLREFLGAAQEFDLNRAAAAVAAAANPPSDVPPEVDAAPLTADVPA
LQAFLEQISLVGEADAEVGDGRVALMTLHAAKGLEFDAVFLTGMEENVFPHSRAVKSESTFLNEVASADGEPSEEMAEER
RLCYVGFTRARKRLFVSLAQCRSLFGELRYNAPSRFLRDVPPALFGISEQEVPEAPRPMVPSTQRRRTWDEDDGPRIDRS
YSQASSDMDGVGGDVRGMRVRHEQFGVGRVVSADGNGPNAKVTVEFGGGVGLKRIIARFLIPG

Sequences:

>Translated_783_residues
MSPANPHESALLEDLNAPQREAVLHGDGPLLVLSGAGSGKTRVITRRVAYLVKVHNVYPWRILAVTFTNKAAREMRERLV
QLLGAQANELVVSTFHSAAAQILRREAEHVGLTRSFVIYDDSDQLNVVKRAMREAGIESMQPREILHRIDQEKNAARLPE
HMQVAPGDERGQLVRKVYAAYQERLRAANAVDFGDLLLLLVTLFRTRPDVLENYRRRFHHVLVDEFQDTNPVQYALLKQL
APPPSANLVVVGDDDQSIYRWRGADVDNILGFPRQYPGAKVVKLEQNYRSDQNILDAAHEVIRKNTRRMEKKLWSERQRG
QTLNLMLNRDERAEGQEVARQILALQREGFIKLSSMAVFYRANAQSRVLEEALRLARVPYTLVSGRSFYDRAEVRDASAY
LRLMVNPRSDADLLRIINIPARGIGDTTVERVTDFANARGVSLYEVLSQPEQIPALNGAAVKRLKGFRNLLESLTAFAQT
NEEAAGAVDQMLRETKLVETLQAEGSDESQTRAENLREFLGAAQEFDLNRAAAAVAAAANPPSDVPPEVDAAPLTADVPA
LQAFLEQISLVGEADAEVGDGRVALMTLHAAKGLEFDAVFLTGMEENVFPHSRAVKSESTFLNEVASADGEPSEEMAEER
RLCYVGFTRARKRLFVSLAQCRSLFGELRYNAPSRFLRDVPPALFGISEQEVPEAPRPMVPSTQRRRTWDEDDGPRIDRS
YSQASSDMDGVGGDVRGMRVRHEQFGVGRVVSADGNGPNAKVTVEFGGGVGLKRIIARFLIPG
>Mature_782_residues
SPANPHESALLEDLNAPQREAVLHGDGPLLVLSGAGSGKTRVITRRVAYLVKVHNVYPWRILAVTFTNKAAREMRERLVQ
LLGAQANELVVSTFHSAAAQILRREAEHVGLTRSFVIYDDSDQLNVVKRAMREAGIESMQPREILHRIDQEKNAARLPEH
MQVAPGDERGQLVRKVYAAYQERLRAANAVDFGDLLLLLVTLFRTRPDVLENYRRRFHHVLVDEFQDTNPVQYALLKQLA
PPPSANLVVVGDDDQSIYRWRGADVDNILGFPRQYPGAKVVKLEQNYRSDQNILDAAHEVIRKNTRRMEKKLWSERQRGQ
TLNLMLNRDERAEGQEVARQILALQREGFIKLSSMAVFYRANAQSRVLEEALRLARVPYTLVSGRSFYDRAEVRDASAYL
RLMVNPRSDADLLRIINIPARGIGDTTVERVTDFANARGVSLYEVLSQPEQIPALNGAAVKRLKGFRNLLESLTAFAQTN
EEAAGAVDQMLRETKLVETLQAEGSDESQTRAENLREFLGAAQEFDLNRAAAAVAAAANPPSDVPPEVDAAPLTADVPAL
QAFLEQISLVGEADAEVGDGRVALMTLHAAKGLEFDAVFLTGMEENVFPHSRAVKSESTFLNEVASADGEPSEEMAEERR
LCYVGFTRARKRLFVSLAQCRSLFGELRYNAPSRFLRDVPPALFGISEQEVPEAPRPMVPSTQRRRTWDEDDGPRIDRSY
SQASSDMDGVGGDVRGMRVRHEQFGVGRVVSADGNGPNAKVTVEFGGGVGLKRIIARFLIPG

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=779, Percent_Identity=38.8960205391528, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI48994965, Length=672, Percent_Identity=36.0119047619048, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1787196, Length=360, Percent_Identity=29.1666666666667, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6322369, Length=751, Percent_Identity=28.4953395472703, Blast_Score=218, Evalue=3e-57,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 87141; Mature: 87010

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPANPHESALLEDLNAPQREAVLHGDGPLLVLSGAGSGKTRVITRRVAYLVKVHNVYPW
CCCCCCHHHHHHHHCCCCHHHEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCE
RILAVTFTNKAAREMRERLVQLLGAQANELVVSTFHSAAAQILRREAEHVGLTRSFVIYD
EEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
DSDQLNVVKRAMREAGIESMQPREILHRIDQEKNAARLPEHMQVAPGDERGQLVRKVYAA
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHH
YQERLRAANAVDFGDLLLLLVTLFRTRPDVLENYRRRFHHVLVDEFQDTNPVQYALLKQL
HHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
APPPSANLVVVGDDDQSIYRWRGADVDNILGFPRQYPGAKVVKLEQNYRSDQNILDAAHE
CCCCCCCEEEEECCCCCCCEECCCCHHHHHCCCCCCCCCEEEEEHHHCCCCHHHHHHHHH
VIRKNTRRMEKKLWSERQRGQTLNLMLNRDERAEGQEVARQILALQREGFIKLSSMAVFY
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEE
RANAQSRVLEEALRLARVPYTLVSGRSFYDRAEVRDASAYLRLMVNPRSDADLLRIINIP
ECCCHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHEEEEEECCCCCCCHHHEEECC
ARGIGDTTVERVTDFANARGVSLYEVLSQPEQIPALNGAAVKRLKGFRNLLESLTAFAQT
CCCCCHHHHHHHHHHHCCCCCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
NEEAAGAVDQMLRETKLVETLQAEGSDESQTRAENLREFLGAAQEFDLNRAAAAVAAAAN
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHCCC
PPSDVPPEVDAAPLTADVPALQAFLEQISLVGEADAEVGDGRVALMTLHAAKGLEFDAVF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEHHCCCCEEEEE
LTGMEENVFPHSRAVKSESTFLNEVASADGEPSEEMAEERRLCYVGFTRARKRLFVSLAQ
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEEEEEHHHHHHHHHHHHHH
CRSLFGELRYNAPSRFLRDVPPALFGISEQEVPEAPRPMVPSTQRRRTWDEDDGPRIDRS
HHHHHHHHCCCCHHHHHHHCCHHHHCCCHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCH
YSQASSDMDGVGGDVRGMRVRHEQFGVGRVVSADGNGPNAKVTVEFGGGVGLKRIIARFL
HHHHCCCCCCCCCCCCCCEEEHHHCCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHC
IPG
CCC
>Mature Secondary Structure 
SPANPHESALLEDLNAPQREAVLHGDGPLLVLSGAGSGKTRVITRRVAYLVKVHNVYPW
CCCCCHHHHHHHHCCCCHHHEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCE
RILAVTFTNKAAREMRERLVQLLGAQANELVVSTFHSAAAQILRREAEHVGLTRSFVIYD
EEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
DSDQLNVVKRAMREAGIESMQPREILHRIDQEKNAARLPEHMQVAPGDERGQLVRKVYAA
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHH
YQERLRAANAVDFGDLLLLLVTLFRTRPDVLENYRRRFHHVLVDEFQDTNPVQYALLKQL
HHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
APPPSANLVVVGDDDQSIYRWRGADVDNILGFPRQYPGAKVVKLEQNYRSDQNILDAAHE
CCCCCCCEEEEECCCCCCCEECCCCHHHHHCCCCCCCCCEEEEEHHHCCCCHHHHHHHHH
VIRKNTRRMEKKLWSERQRGQTLNLMLNRDERAEGQEVARQILALQREGFIKLSSMAVFY
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEE
RANAQSRVLEEALRLARVPYTLVSGRSFYDRAEVRDASAYLRLMVNPRSDADLLRIINIP
ECCCHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHEEEEEECCCCCCCHHHEEECC
ARGIGDTTVERVTDFANARGVSLYEVLSQPEQIPALNGAAVKRLKGFRNLLESLTAFAQT
CCCCCHHHHHHHHHHHCCCCCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
NEEAAGAVDQMLRETKLVETLQAEGSDESQTRAENLREFLGAAQEFDLNRAAAAVAAAAN
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHCCC
PPSDVPPEVDAAPLTADVPALQAFLEQISLVGEADAEVGDGRVALMTLHAAKGLEFDAVF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEHHCCCCEEEEE
LTGMEENVFPHSRAVKSESTFLNEVASADGEPSEEMAEERRLCYVGFTRARKRLFVSLAQ
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEEEEEHHHHHHHHHHHHHH
CRSLFGELRYNAPSRFLRDVPPALFGISEQEVPEAPRPMVPSTQRRRTWDEDDGPRIDRS
HHHHHHHHCCCCHHHHHHHCCHHHHCCCHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCH
YSQASSDMDGVGGDVRGMRVRHEQFGVGRVVSADGNGPNAKVTVEFGGGVGLKRIIARFL
HHHHCCCCCCCCCCCCCCEEEHHHCCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHC
IPG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]