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The map label for this gene is pleD [H]

Identifier: 108757503

GI number: 108757503

Start: 1787726

End: 1788790

Strand: Direct

Name: pleD [H]

Synonym: MXAN_1525

Alternate gene names: 108757503

Gene position: 1787726-1788790 (Clockwise)

Preceding gene: 108758591

Following gene: 108761423

Centisome position: 19.56

GC content: 63.76

Gene sequence:

>1065_bases
ATGCCGAACCGCCTGTTCAGGTGTCCTGACCGGCGCTGGACGTCCCGACGCCTCGGGTTGCCGGGCCTAGCCCGGCGGCC
GCTCCCGGGTGGCATGTGGGAGAAGCGCCGCAAAGGGCCGAAGAACCGGCCCCCGCTGCGTTGCATGGCGTCTGCCGAGG
CTATCGTGCTAGCCTCGTTTCCGATGGCCGGCGACGAAACCCGCGTCACCAAGATCTCAACGCTCAACGTGCATGCCCAC
CGCAGCACGGAGTGTTGTCTCGTGCAGATTCATGGCCCCGAGCTCGGCAAGAAGTACCTCATCGAGGATGCCGAACTCAC
CATTGGGCGGGATCAGCACAACCACATCGTCGTGGACCTGGACAACGTGTCCCGCCGCCACGCCCGGATTCTGGGGCGTG
GGGGGAAGATGCTCGTTGAAGACCTGGGCTCCACCAACGGTACCTTCCTCAATGACCAGGAAGTGCTTCAGGCCTCTCCG
CTGCGCAGCGGAGACCTGGTCAAGGTCGGAGGCTCCATCTTCAAGTTCCTCGATGGCGACAACATCGAGACCCAGTACCA
CGAGACCATCTACACGCTGACCATCGCGGACGGTCTCACCGGCATCAACAACAAGAGGTACTTCCTGGAGTACCTCGAGA
AGGAGATGGGCCGGTCGACCCGCTATCAGCGCACCCTCACGCTGATGATGTTCGACATCGACCACTTCAAGCAGATCAAC
GACGTCCACGGCCACCTCGCCGGGGACTACGTGCTGCGGGAGTTGGCCCAGTCCATCAAGCGCCTGGTGCGCCGCGAGCA
GTGCTTCGCCCGCTACGGCGGCGAGGAGTTCGCCGTCGTCCTCCCGGAGGACGGCCCGGACAAGGCGCGCCTGTTCGCGG
AGAAGATTCGCCGGCTCATCGAGGGCAAGTCCTTCGTCTACGACGACAAGGAAATCCCCGTCACCATCTCCATTGGCGTG
GCCGAGCAGACGTCGGACATGCTGGAGCCCACGCACTTCATCAAGGTGGCGGACGCCAACCTGTACAAGGCGAAGAAGTC
GGGCCGCAACCGCGTGGTGGGCTAG

Upstream 100 bases:

>100_bases
CCAGAATCATCGCCAGGACTTTGGACATAGGTGTTCCGGACGTTAGTCGTCCCTGGGGGGCCGGCAAGGGCACGTGTGCC
CGCCTGCCAGCCATCGTTGA

Downstream 100 bases:

>100_bases
GTCGTGGCCGCCAGCGCAAGCGAAGCGGGTCCGTCCTTCCTCGCGCGGTTGGTGCTCGTGCTCCTGGGCTGCGCCTTCCT
CGGCGCGGCCTGGGTGTGGA

Product: FHA domain/GGDEF domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MPNRLFRCPDRRWTSRRLGLPGLARRPLPGGMWEKRRKGPKNRPPLRCMASAEAIVLASFPMAGDETRVTKISTLNVHAH
RSTECCLVQIHGPELGKKYLIEDAELTIGRDQHNHIVVDLDNVSRRHARILGRGGKMLVEDLGSTNGTFLNDQEVLQASP
LRSGDLVKVGGSIFKFLDGDNIETQYHETIYTLTIADGLTGINNKRYFLEYLEKEMGRSTRYQRTLTLMMFDIDHFKQIN
DVHGHLAGDYVLRELAQSIKRLVRREQCFARYGGEEFAVVLPEDGPDKARLFAEKIRRLIEGKSFVYDDKEIPVTISIGV
AEQTSDMLEPTHFIKVADANLYKAKKSGRNRVVG

Sequences:

>Translated_354_residues
MPNRLFRCPDRRWTSRRLGLPGLARRPLPGGMWEKRRKGPKNRPPLRCMASAEAIVLASFPMAGDETRVTKISTLNVHAH
RSTECCLVQIHGPELGKKYLIEDAELTIGRDQHNHIVVDLDNVSRRHARILGRGGKMLVEDLGSTNGTFLNDQEVLQASP
LRSGDLVKVGGSIFKFLDGDNIETQYHETIYTLTIADGLTGINNKRYFLEYLEKEMGRSTRYQRTLTLMMFDIDHFKQIN
DVHGHLAGDYVLRELAQSIKRLVRREQCFARYGGEEFAVVLPEDGPDKARLFAEKIRRLIEGKSFVYDDKEIPVTISIGV
AEQTSDMLEPTHFIKVADANLYKAKKSGRNRVVG
>Mature_353_residues
PNRLFRCPDRRWTSRRLGLPGLARRPLPGGMWEKRRKGPKNRPPLRCMASAEAIVLASFPMAGDETRVTKISTLNVHAHR
STECCLVQIHGPELGKKYLIEDAELTIGRDQHNHIVVDLDNVSRRHARILGRGGKMLVEDLGSTNGTFLNDQEVLQASPL
RSGDLVKVGGSIFKFLDGDNIETQYHETIYTLTIADGLTGINNKRYFLEYLEKEMGRSTRYQRTLTLMMFDIDHFKQIND
VHGHLAGDYVLRELAQSIKRLVRREQCFARYGGEEFAVVLPEDGPDKARLFAEKIRRLIEGKSFVYDDKEIPVTISIGVA
EQTSDMLEPTHFIKVADANLYKAKKSGRNRVVG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=159, Percent_Identity=40.8805031446541, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=39.375, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786584, Length=164, Percent_Identity=35.3658536585366, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=35.031847133758, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87081881, Length=159, Percent_Identity=37.1069182389937, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787816, Length=168, Percent_Identity=33.9285714285714, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=36.0248447204969, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=34.7560975609756, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788381, Length=204, Percent_Identity=29.9019607843137, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87081974, Length=157, Percent_Identity=29.9363057324841, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787541, Length=179, Percent_Identity=27.3743016759777, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081977, Length=181, Percent_Identity=27.0718232044199, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788956, Length=158, Percent_Identity=29.1139240506329, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787056, Length=154, Percent_Identity=31.8181818181818, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 40145; Mature: 40014

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50006 FHA_DOMAIN ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNRLFRCPDRRWTSRRLGLPGLARRPLPGGMWEKRRKGPKNRPPLRCMASAEAIVLASF
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEECCCCEEEEEEC
PMAGDETRVTKISTLNVHAHRSTECCLVQIHGPELGKKYLIEDAELTIGRDQHNHIVVDL
CCCCCCCEEEEEEEEEEEECCCCCEEEEEEECCCCCCHHEEECCEEEECCCCCCEEEEEC
DNVSRRHARILGRGGKMLVEDLGSTNGTFLNDQEVLQASPLRSGDLVKVGGSIFKFLDGD
CCCCHHHHHHHCCCCHHHHHHCCCCCCCEECCHHHHHCCCCCCCCEEEECCHHHHHHCCC
NIETQYHETIYTLTIADGLTGINNKRYFLEYLEKEMGRSTRYQRTLTLMMFDIDHFKQIN
CCCCEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHEEEEEEECHHHHHHHH
DVHGHLAGDYVLRELAQSIKRLVRREQCFARYGGEEFAVVLPEDGPDKARLFAEKIRRLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHH
EGKSFVYDDKEIPVTISIGVAEQTSDMLEPTHFIKVADANLYKAKKSGRNRVVG
CCCCEEECCCCCCEEEEECCCHHHHHHCCCCEEEEEECCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PNRLFRCPDRRWTSRRLGLPGLARRPLPGGMWEKRRKGPKNRPPLRCMASAEAIVLASF
CCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEECCCCEEEEEEC
PMAGDETRVTKISTLNVHAHRSTECCLVQIHGPELGKKYLIEDAELTIGRDQHNHIVVDL
CCCCCCCEEEEEEEEEEEECCCCCEEEEEEECCCCCCHHEEECCEEEECCCCCCEEEEEC
DNVSRRHARILGRGGKMLVEDLGSTNGTFLNDQEVLQASPLRSGDLVKVGGSIFKFLDGD
CCCCHHHHHHHCCCCHHHHHHCCCCCCCEECCHHHHHCCCCCCCCEEEECCHHHHHHCCC
NIETQYHETIYTLTIADGLTGINNKRYFLEYLEKEMGRSTRYQRTLTLMMFDIDHFKQIN
CCCCEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHEEEEEEECHHHHHHHH
DVHGHLAGDYVLRELAQSIKRLVRREQCFARYGGEEFAVVLPEDGPDKARLFAEKIRRLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHH
EGKSFVYDDKEIPVTISIGVAEQTSDMLEPTHFIKVADANLYKAKKSGRNRVVG
CCCCEEECCCCCCEEEEECCCHHHHHHCCCCEEEEEECCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA