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The map label for this gene is rrmJ

Identifier: 108757449

GI number: 108757449

Start: 3482305

End: 3482946

Strand: Direct

Name: rrmJ

Synonym: MXAN_2973

Alternate gene names: 108757449

Gene position: 3482305-3482946 (Clockwise)

Preceding gene: 108762147

Following gene: 108759486

Centisome position: 38.1

GC content: 67.29

Gene sequence:

>642_bases
ATGCTAGGGAGCCCCCCTGACATGGGCAAGCCCTACCGTCCTAAAGACCACTATTTCCAGAAGGCCAAGCAAGAGGGCCT
GCGGGCACGTTCGGCCTTCAAGGTTGATGAACTCATCAAGCGCTTCCCCATGGTGAAGAAGGGGCACGTCGTGCTCGACC
TGGGGGCGGCGCCAGGAGGTTTCCTCCAGATATTGGCGGACGCCGTGGGGCCGAAGGGCCGCGTCATCGGCGTGGACATC
GTCGCCATCCGTCCCTTCAGCCAGCCCTTCGTGCAGACGGCGGTGCTGGACGTGCTCGCGGATGACTTCGACGCGAAGCT
GACCGAGCTCCATGCCGGCCCCTTCGACGCCGTCATCTCCGACATGGCCCCCAAGACGAGCGGCATCAAGGCCACCGACG
AGGCGCGCAGCCTGCGGCTGGCGGGCAAGGCGCTGGAGTTGGCCGCGGCGCGGGGGCGGCCCGGGTCGTCCTTCGTGGCC
AAGGTCTTCATGGGCCGGGACTTCGAGGACTTCCGCAACCAGATCCGCGCCCTCTTCGAAGAGGTGAAGGTGGTCCGGCC
GGAAGCCACCCGCGGCGCCAGCATGGAGGTCTACCTGGTGGGTCTTCGCCGCCGTGCGCCAGAGGCCCCGGAGGCGAACT
GA

Upstream 100 bases:

>100_bases
TCATCACGCTCCGGGTCGTGCCCATCCTACGTCCTCCGGACTGGCCGTTGCACGGGGAGTGACACCCGCGACTGCTCCGG
CTTCCCGAAGACAGTAGCGG

Downstream 100 bases:

>100_bases
CGTTCATTGTGGAACGTTCGGGTGTCCAGCTGTGTCTAGAGTGCCGACATGCATCGGACCCCCTGGACCCGCGCACCCTT
TGTTCGCTTCTGGCTGTTCG

Product: ribosomal RNA large subunit methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MLGSPPDMGKPYRPKDHYFQKAKQEGLRARSAFKVDELIKRFPMVKKGHVVLDLGAAPGGFLQILADAVGPKGRVIGVDI
VAIRPFSQPFVQTAVLDVLADDFDAKLTELHAGPFDAVISDMAPKTSGIKATDEARSLRLAGKALELAAARGRPGSSFVA
KVFMGRDFEDFRNQIRALFEEVKVVRPEATRGASMEVYLVGLRRRAPEAPEAN

Sequences:

>Translated_213_residues
MLGSPPDMGKPYRPKDHYFQKAKQEGLRARSAFKVDELIKRFPMVKKGHVVLDLGAAPGGFLQILADAVGPKGRVIGVDI
VAIRPFSQPFVQTAVLDVLADDFDAKLTELHAGPFDAVISDMAPKTSGIKATDEARSLRLAGKALELAAARGRPGSSFVA
KVFMGRDFEDFRNQIRALFEEVKVVRPEATRGASMEVYLVGLRRRAPEAPEAN
>Mature_213_residues
MLGSPPDMGKPYRPKDHYFQKAKQEGLRARSAFKVDELIKRFPMVKKGHVVLDLGAAPGGFLQILADAVGPKGRVIGVDI
VAIRPFSQPFVQTAVLDVLADDFDAKLTELHAGPFDAVISDMAPKTSGIKATDEARSLRLAGKALELAAARGRPGSSFVA
KVFMGRDFEDFRNQIRALFEEVKVVRPEATRGASMEVYLVGLRRRAPEAPEAN

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI194097365, Length=200, Percent_Identity=35, Blast_Score=111, Evalue=4e-25,
Organism=Homo sapiens, GI29029591, Length=205, Percent_Identity=34.1463414634146, Blast_Score=108, Evalue=2e-24,
Organism=Homo sapiens, GI29029589, Length=205, Percent_Identity=34.1463414634146, Blast_Score=108, Evalue=2e-24,
Organism=Homo sapiens, GI7110661, Length=205, Percent_Identity=34.1463414634146, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI7019377, Length=199, Percent_Identity=31.6582914572864, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1789569, Length=191, Percent_Identity=40.3141361256544, Blast_Score=135, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17553860, Length=200, Percent_Identity=32.5, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71987550, Length=200, Percent_Identity=32.5, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71987561, Length=200, Percent_Identity=32.5, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17553474, Length=193, Percent_Identity=32.1243523316062, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17554650, Length=206, Percent_Identity=29.6116504854369, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6319535, Length=212, Percent_Identity=33.9622641509434, Blast_Score=109, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319796, Length=195, Percent_Identity=32.3076923076923, Blast_Score=94, Evalue=1e-20,
Organism=Drosophila melanogaster, GI18859957, Length=195, Percent_Identity=34.3589743589744, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356387, Length=196, Percent_Identity=30.1020408163265, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24647580, Length=217, Percent_Identity=29.0322580645161, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648639, Length=204, Percent_Identity=28.4313725490196, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_MYXXD (Q1D841)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_631182.1
- ProteinModelPortal:   Q1D841
- SMR:   Q1D841
- STRING:   Q1D841
- GeneID:   4105227
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_2973
- TIGR:   MXAN_2973
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   Q1D841
- BioCyc:   MXAN246197:MXAN_2973-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23215; Mature: 23215

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: ACT_SITE 161-161 BINDING 59-59 BINDING 61-61 BINDING 79-79 BINDING 102-102 BINDING 121-121

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGSPPDMGKPYRPKDHYFQKAKQEGLRARSAFKVDELIKRFPMVKKGHVVLDLGAAPGG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCEEEEECCCCHH
FLQILADAVGPKGRVIGVDIVAIRPFSQPFVQTAVLDVLADDFDAKLTELHAGPFDAVIS
HHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHH
DMAPKTSGIKATDEARSLRLAGKALELAAARGRPGSSFVAKVFMGRDFEDFRNQIRALFE
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
EVKVVRPEATRGASMEVYLVGLRRRAPEAPEAN
HHHHCCCCCCCCCCEEEEEEEEHHCCCCCCCCC
>Mature Secondary Structure
MLGSPPDMGKPYRPKDHYFQKAKQEGLRARSAFKVDELIKRFPMVKKGHVVLDLGAAPGG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCEEEEECCCCHH
FLQILADAVGPKGRVIGVDIVAIRPFSQPFVQTAVLDVLADDFDAKLTELHAGPFDAVIS
HHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHH
DMAPKTSGIKATDEARSLRLAGKALELAAARGRPGSSFVAKVFMGRDFEDFRNQIRALFE
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
EVKVVRPEATRGASMEVYLVGLRRRAPEAPEAN
HHHHCCCCCCCCCCEEEEEEEEHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA