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The map label for this gene is citZ [H]

Identifier: 108757424

GI number: 108757424

Start: 467571

End: 468875

Strand: Reverse

Name: citZ [H]

Synonym: MXAN_0407

Alternate gene names: 108757424

Gene position: 468875-467571 (Counterclockwise)

Preceding gene: 108759112

Following gene: 108757257

Centisome position: 5.13

GC content: 71.8

Gene sequence:

>1305_bases
ATGAGTAGGCCCAAGGGCGGACGCTCTCAATCTCGATTCGACCATCGACCTGAGGAGGAGCTCGTGACCGCGGCACAGGC
CGCCGCGCTCCTGGGCGTGAAGCGAGCCACGCTCTACACCTACGTGAGCCGAGGCCTCGTGCGCTGCGTGCCGGAGCGAG
GCACCAAGGAGAACCGCTATGTGCGCTCCGACCTGGAGCGCCTGAAAGCGCGGCACGACGCGAGAGCGGGACACGCGGCC
GTCGCCTCGGGCGCCTTGCGCTGGGGCGAGCCCGTCATCGACTCGTCGGTGTCCCGCGTGGGCGCGGAGGGGCTCGCATA
CCGAGGCCATTCGGCGGTGACGCTCGCGGTGGAGGGACGCGCGTTCGAGGACGTGGCCGAGCTGTTGTGGACAGGCACGC
TGCCCGAAGAAGCGTCACGGTGGCCCTCACCCGAGCCGGCGTTTCCACCGTCCGAGCTGGCGAAGCTGTTGCCCCGTGGA
ACACCTCCCGTTGCCGCGCTGTCTGCGTTGGTGCCGCTCCTAGGCGCAAGGGACTCGGCGCGCTTTGCCTCTCCTCCCGA
GCAGGAGCGGGCCCGCGCCCGGAGGCTGCTGCGCCACCTGTCCGCCTGGGTCTGCGTGGCGAACGCGCCGGGGCGAGTGA
CGCATGCCTTGAACGAGCCCACGGTGGCGCAATCCCTGGCGCGCGCTTGGAACACCCGCGTGAAGCGCGCACCTGAGCTG
CTCAACCGCGCGCTGGTGCTGTGCGCGGACCATGAGCTGAACGTGTCCACGTTCACCGCGCGCGTGGCGGCCTCCTCCAG
CGCGGACCTGTACGCCTGCTTGAGCGCCGCGCTGGCCGCGCTCTCCGGTCCCAAGCACGGAGGGGCCTGCGACCGCGTGG
AGGCGTTGCTCGTGGAAGTGGGCCGGCCGGAGCGCGCGGCGGAGGTCATCCGGGAGCGGCTGCGGCGAGGCGAAGCGGTC
CCCGGCTTCGGTCATCAGCTCTATCCAGACGGAGACCCACGGACACCACCGCTGGTGGAAGCCGCGCGGAGCGTCAGGCC
CGAGACGCCCAATGTCCGTATCGCCAGCGCCGTGCTGGAAGCCATGCGCGCGGCGGGACATCCGCCCCCGTCCGTGGACT
TCGGGCTGGTGATGCTCACCGGAGCGCTGGGGCTTCCTTCAGGCGCGGGCGCCACGCTGTTCGCCGTCGGGCGTGCCGCG
GGCTGGGTGGCCCATGTGCTGGAGCAGCGGGAACAGGGCCACCTGCTGCGCCCTCGGGCACGCTACGTGGAGCCGGCACA
CCCCGCCGGTGGCAGGGCGCCATGA

Upstream 100 bases:

>100_bases
CTCCTCTCCTCGCGGGCACACGCGGGCCCCGCCGACCTGGGGACGTATCGACACGTCATCCAGTCGAGTCGACAATGGCC
TTCGCGGAGGTGCGCGAGAC

Downstream 100 bases:

>100_bases
ACACGCTCCGCGCATCCGGACGCCTCACGCACGTCACGTGAGCACTCAGCCCACTGTCGCATGCCACGCACCGGTCGAAG
TCCGTCGTTGGAGGTCCGCG

Product: citrate synthase

Products: NA

Alternate protein names: Citrate synthase II [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSRPKGGRSQSRFDHRPEEELVTAAQAAALLGVKRATLYTYVSRGLVRCVPERGTKENRYVRSDLERLKARHDARAGHAA
VASGALRWGEPVIDSSVSRVGAEGLAYRGHSAVTLAVEGRAFEDVAELLWTGTLPEEASRWPSPEPAFPPSELAKLLPRG
TPPVAALSALVPLLGARDSARFASPPEQERARARRLLRHLSAWVCVANAPGRVTHALNEPTVAQSLARAWNTRVKRAPEL
LNRALVLCADHELNVSTFTARVAASSSADLYACLSAALAALSGPKHGGACDRVEALLVEVGRPERAAEVIRERLRRGEAV
PGFGHQLYPDGDPRTPPLVEAARSVRPETPNVRIASAVLEAMRAAGHPPPSVDFGLVMLTGALGLPSGAGATLFAVGRAA
GWVAHVLEQREQGHLLRPRARYVEPAHPAGGRAP

Sequences:

>Translated_434_residues
MSRPKGGRSQSRFDHRPEEELVTAAQAAALLGVKRATLYTYVSRGLVRCVPERGTKENRYVRSDLERLKARHDARAGHAA
VASGALRWGEPVIDSSVSRVGAEGLAYRGHSAVTLAVEGRAFEDVAELLWTGTLPEEASRWPSPEPAFPPSELAKLLPRG
TPPVAALSALVPLLGARDSARFASPPEQERARARRLLRHLSAWVCVANAPGRVTHALNEPTVAQSLARAWNTRVKRAPEL
LNRALVLCADHELNVSTFTARVAASSSADLYACLSAALAALSGPKHGGACDRVEALLVEVGRPERAAEVIRERLRRGEAV
PGFGHQLYPDGDPRTPPLVEAARSVRPETPNVRIASAVLEAMRAAGHPPPSVDFGLVMLTGALGLPSGAGATLFAVGRAA
GWVAHVLEQREQGHLLRPRARYVEPAHPAGGRAP
>Mature_433_residues
SRPKGGRSQSRFDHRPEEELVTAAQAAALLGVKRATLYTYVSRGLVRCVPERGTKENRYVRSDLERLKARHDARAGHAAV
ASGALRWGEPVIDSSVSRVGAEGLAYRGHSAVTLAVEGRAFEDVAELLWTGTLPEEASRWPSPEPAFPPSELAKLLPRGT
PPVAALSALVPLLGARDSARFASPPEQERARARRLLRHLSAWVCVANAPGRVTHALNEPTVAQSLARAWNTRVKRAPELL
NRALVLCADHELNVSTFTARVAASSSADLYACLSAALAALSGPKHGGACDRVEALLVEVGRPERAAEVIRERLRRGEAVP
GFGHQLYPDGDPRTPPLVEAARSVRPETPNVRIASAVLEAMRAAGHPPPSVDFGLVMLTGALGLPSGAGATLFAVGRAAG
WVAHVLEQREQGHLLRPRARYVEPAHPAGGRAP

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=339, Percent_Identity=28.6135693215339, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786527, Length=366, Percent_Identity=29.5081967213115, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1786939, Length=358, Percent_Identity=28.7709497206704, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17555174, Length=200, Percent_Identity=32, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324328, Length=220, Percent_Identity=27.7272727272727, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21356863, Length=195, Percent_Identity=27.6923076923077, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 46368; Mature: 46237

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPKGGRSQSRFDHRPEEELVTAAQAAALLGVKRATLYTYVSRGLVRCVPERGTKENRY
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
VRSDLERLKARHDARAGHAAVASGALRWGEPVIDSSVSRVGAEGLAYRGHSAVTLAVEGR
HHHHHHHHHHHHHHCCCHHHHHHCCHHCCCHHHHHHHHHHCCHHHEECCCCEEEEEECCC
AFEDVAELLWTGTLPEEASRWPSPEPAFPPSELAKLLPRGTPPVAALSALVPLLGARDSA
CHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
RFASPPEQERARARRLLRHLSAWVCVANAPGRVTHALNEPTVAQSLARAWNTRVKRAPEL
CCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LNRALVLCADHELNVSTFTARVAASSSADLYACLSAALAALSGPKHGGACDRVEALLVEV
HHHHEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
GRPERAAEVIRERLRRGEAVPGFGHQLYPDGDPRTPPLVEAARSVRPETPNVRIASAVLE
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHH
AMRAAGHPPPSVDFGLVMLTGALGLPSGAGATLFAVGRAAGWVAHVLEQREQGHLLRPRA
HHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
RYVEPAHPAGGRAP
HCCCCCCCCCCCCC
>Mature Secondary Structure 
SRPKGGRSQSRFDHRPEEELVTAAQAAALLGVKRATLYTYVSRGLVRCVPERGTKENRY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
VRSDLERLKARHDARAGHAAVASGALRWGEPVIDSSVSRVGAEGLAYRGHSAVTLAVEGR
HHHHHHHHHHHHHHCCCHHHHHHCCHHCCCHHHHHHHHHHCCHHHEECCCCEEEEEECCC
AFEDVAELLWTGTLPEEASRWPSPEPAFPPSELAKLLPRGTPPVAALSALVPLLGARDSA
CHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
RFASPPEQERARARRLLRHLSAWVCVANAPGRVTHALNEPTVAQSLARAWNTRVKRAPEL
CCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LNRALVLCADHELNVSTFTARVAASSSADLYACLSAALAALSGPKHGGACDRVEALLVEV
HHHHEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
GRPERAAEVIRERLRRGEAVPGFGHQLYPDGDPRTPPLVEAARSVRPETPNVRIASAVLE
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHH
AMRAAGHPPPSVDFGLVMLTGALGLPSGAGATLFAVGRAAGWVAHVLEQREQGHLLRPRA
HHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
RYVEPAHPAGGRAP
HCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377 [H]