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The map label for this gene is pbpE [H]

Identifier: 108757288

GI number: 108757288

Start: 2465541

End: 2466953

Strand: Reverse

Name: pbpE [H]

Synonym: MXAN_2136

Alternate gene names: 108757288

Gene position: 2466953-2465541 (Counterclockwise)

Preceding gene: 108760229

Following gene: 108758715

Centisome position: 26.99

GC content: 64.33

Gene sequence:

>1413_bases
ATGCGCGGTTTCTTGCAATCCATCACATTGGTTGCTGTCTTCCTTGTAGGCCTGTTCACGAGCCGCCCCGCCGCCGCACA
TGGTTCGCTCCGCGAGCGGATCGACACCTTCGTCCGCGCGGAGCAGAAACGACAGAATGTGCCCGGCCTCGCCGTGGGCG
TCGTGAGCCACGGAAGGGTCGTGCTGGCAAAGGGGTATGGCTTTGCCAATCTGGAGCATCGGGTTCCTGTCAGCAAGGAC
ACCCTCTTCCAATCTGGCTCCCTGGGCAAACAATTCACCGCAATGGCGGTGATGCACCAGGTGGAGGCAGGCAGGATTTC
GCTCTCCGACAGCATCACGAAGTTCTTCCCCGACGCGCCCACCAAGTGGCACCCCATCACAGTGCGCCACCTGCTGACGC
ACACCTCCGGCATCCAGGACCTCGAGGGACTGCTCGACGTGCGCAAGGACTACACGGACGAGGAATTCGCGCACTTCATC
TACGAGTTGCCCTTGGAGTTTCCCGCCGGTCTGCGGTGGAACTACAGCAACTCCGGCTACGTCCTCCTGGGCATCATCGT
GAACCGCGTCGCGGGCACCGACTACACCAACGTCCTCGGTGAGCACGTCTTCCGGCCCGCCGGAATGAAGACGGCGCGCG
GAATCAGCGAGGCGGACATCATTCCCAACCGCTCGGCCGGCTACCGCGTGGTCCGCGGCGAGGTGAAGAACCAGGAGTGG
GTTTCGCCTTCCCTCAACACCACCGGGGACGGTGCGCTCTATTTCTCCGTGAAGGACTTGCTGGCCTGGGATGCCGCCGT
GGCGCAGCGCTCCATCCTGAGCCCGGAGAGCTGGCAGGAAATCCTGTCCCCCGTGAAGCTCAACAGCGGAGCCACCCAGC
CCTATGGCTTTGGATGGGAAATCCTCGAGCGCAATGGCAAGCCCCTGCACCAGCATACCGGTGGGTGGCAGGGCTTCCGG
ACCGCCTACTCCCGCTTCATCGGGGACTCTCTCTCCATCGTCGTGCTGGCCAACTCGAGTGGGTCAAACCCCATGACCTT
CGTGGAAGGCATTGCTGCCATCGTCAACCCCGCGCTCGCCGTGCCCCCGTTGACCCCCATCCCGGACCTCGAGCCGCAGG
TCACCGCCCGGCTCACCACGCTGCTCGAGCAGGCACGGGCCGGGACGATGGAGCCAGTCGAGTTCGCGTACGCCCCCTGG
TGGTTCTGGGGCCAAGCCGTGCCGTTCTATCAGCAACTGCTGCAAAGCCTGGCGCCGGCGGGGCCGCTCGTCCTGGTCAA
GCGGGAAGTGGTGGGTGACGACCGCCTCTACACGTACCTGGTGCAGTTCGGGCCCAAGACCCTCCGCTACAGAGTGTCGC
TCATCCCGGATGGCCGGGTGTCCGCCTTCCGCCTGAACGAGCACATCCCCTAA

Upstream 100 bases:

>100_bases
TCAAAAAGGGAAATTCACCACAATTATTTACAGCACAGAGGGAGCAATGTTCTCGTGAGCATACCCGTGACCCATACCCG
TCCGGGCATGAGGAGCTCCC

Downstream 100 bases:

>100_bases
GGCCTCGGACTACGACCTCGGACTGTACGACGACGACTCCCTTGACGCCGTCGCCCTGGCCCACCTCCGCCGCCTCGCCG
TGGAGCTCGCTGCCTCCGCG

Product: beta-lactamase

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MRGFLQSITLVAVFLVGLFTSRPAAAHGSLRERIDTFVRAEQKRQNVPGLAVGVVSHGRVVLAKGYGFANLEHRVPVSKD
TLFQSGSLGKQFTAMAVMHQVEAGRISLSDSITKFFPDAPTKWHPITVRHLLTHTSGIQDLEGLLDVRKDYTDEEFAHFI
YELPLEFPAGLRWNYSNSGYVLLGIIVNRVAGTDYTNVLGEHVFRPAGMKTARGISEADIIPNRSAGYRVVRGEVKNQEW
VSPSLNTTGDGALYFSVKDLLAWDAAVAQRSILSPESWQEILSPVKLNSGATQPYGFGWEILERNGKPLHQHTGGWQGFR
TAYSRFIGDSLSIVVLANSSGSNPMTFVEGIAAIVNPALAVPPLTPIPDLEPQVTARLTTLLEQARAGTMEPVEFAYAPW
WFWGQAVPFYQQLLQSLAPAGPLVLVKREVVGDDRLYTYLVQFGPKTLRYRVSLIPDGRVSAFRLNEHIP

Sequences:

>Translated_470_residues
MRGFLQSITLVAVFLVGLFTSRPAAAHGSLRERIDTFVRAEQKRQNVPGLAVGVVSHGRVVLAKGYGFANLEHRVPVSKD
TLFQSGSLGKQFTAMAVMHQVEAGRISLSDSITKFFPDAPTKWHPITVRHLLTHTSGIQDLEGLLDVRKDYTDEEFAHFI
YELPLEFPAGLRWNYSNSGYVLLGIIVNRVAGTDYTNVLGEHVFRPAGMKTARGISEADIIPNRSAGYRVVRGEVKNQEW
VSPSLNTTGDGALYFSVKDLLAWDAAVAQRSILSPESWQEILSPVKLNSGATQPYGFGWEILERNGKPLHQHTGGWQGFR
TAYSRFIGDSLSIVVLANSSGSNPMTFVEGIAAIVNPALAVPPLTPIPDLEPQVTARLTTLLEQARAGTMEPVEFAYAPW
WFWGQAVPFYQQLLQSLAPAGPLVLVKREVVGDDRLYTYLVQFGPKTLRYRVSLIPDGRVSAFRLNEHIP
>Mature_470_residues
MRGFLQSITLVAVFLVGLFTSRPAAAHGSLRERIDTFVRAEQKRQNVPGLAVGVVSHGRVVLAKGYGFANLEHRVPVSKD
TLFQSGSLGKQFTAMAVMHQVEAGRISLSDSITKFFPDAPTKWHPITVRHLLTHTSGIQDLEGLLDVRKDYTDEEFAHFI
YELPLEFPAGLRWNYSNSGYVLLGIIVNRVAGTDYTNVLGEHVFRPAGMKTARGISEADIIPNRSAGYRVVRGEVKNQEW
VSPSLNTTGDGALYFSVKDLLAWDAAVAQRSILSPESWQEILSPVKLNSGATQPYGFGWEILERNGKPLHQHTGGWQGFR
TAYSRFIGDSLSIVVLANSSGSNPMTFVEGIAAIVNPALAVPPLTPIPDLEPQVTARLTTLLEQARAGTMEPVEFAYAPW
WFWGQAVPFYQQLLQSLAPAGPLVLVKREVVGDDRLYTYLVQFGPKTLRYRVSLIPDGRVSAFRLNEHIP

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Homo sapiens, GI26051231, Length=369, Percent_Identity=24.6612466124661, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI26051233, Length=250, Percent_Identity=25.2, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1790593, Length=165, Percent_Identity=29.6969696969697, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786574, Length=380, Percent_Identity=23.4210526315789, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17550934, Length=343, Percent_Identity=27.6967930029155, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI115533026, Length=333, Percent_Identity=25.5255255255255, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17565630, Length=196, Percent_Identity=26.530612244898, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 51923; Mature: 51923

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGFLQSITLVAVFLVGLFTSRPAAAHGSLRERIDTFVRAEQKRQNVPGLAVGVVSHGRV
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCE
VLAKGYGFANLEHRVPVSKDTLFQSGSLGKQFTAMAVMHQVEAGRISLSDSITKFFPDAP
EEEECCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCC
TKWHPITVRHLLTHTSGIQDLEGLLDVRKDYTDEEFAHFIYELPLEFPAGLRWNYSNSGY
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEECCCCE
VLLGIIVNRVAGTDYTNVLGEHVFRPAGMKTARGISEADIIPNRSAGYRVVRGEVKNQEW
EEEHHHHHHHCCCCHHHHHHHHHCCCCCCHHHCCCCHHCCCCCCCCCCEEEECCCCCCCC
VSPSLNTTGDGALYFSVKDLLAWDAAVAQRSILSPESWQEILSPVKLNSGATQPYGFGWE
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHCCEECCCCCCCCCCCCHH
ILERNGKPLHQHTGGWQGFRTAYSRFIGDSLSIVVLANSSGSNPMTFVEGIAAIVNPALA
HHHCCCCCCHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCC
VPPLTPIPDLEPQVTARLTTLLEQARAGTMEPVEFAYAPWWFWGQAVPFYQQLLQSLAPA
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEECCHHHHCCCCHHHHHHHHHHCCC
GPLVLVKREVVGDDRLYTYLVQFGPKTLRYRVSLIPDGRVSAFRLNEHIP
CCEEEEEHHHCCCCHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCCCCC
>Mature Secondary Structure
MRGFLQSITLVAVFLVGLFTSRPAAAHGSLRERIDTFVRAEQKRQNVPGLAVGVVSHGRV
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCE
VLAKGYGFANLEHRVPVSKDTLFQSGSLGKQFTAMAVMHQVEAGRISLSDSITKFFPDAP
EEEECCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCC
TKWHPITVRHLLTHTSGIQDLEGLLDVRKDYTDEEFAHFIYELPLEFPAGLRWNYSNSGY
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEECCCCE
VLLGIIVNRVAGTDYTNVLGEHVFRPAGMKTARGISEADIIPNRSAGYRVVRGEVKNQEW
EEEHHHHHHHCCCCHHHHHHHHHCCCCCCHHHCCCCHHCCCCCCCCCCEEEECCCCCCCC
VSPSLNTTGDGALYFSVKDLLAWDAAVAQRSILSPESWQEILSPVKLNSGATQPYGFGWE
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHCCEECCCCCCCCCCCCHH
ILERNGKPLHQHTGGWQGFRTAYSRFIGDSLSIVVLANSSGSNPMTFVEGIAAIVNPALA
HHHCCCCCCHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCC
VPPLTPIPDLEPQVTARLTTLLEQARAGTMEPVEFAYAPWWFWGQAVPFYQQLLQSLAPA
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEECCHHHHCCCCHHHHHHHHHHCCC
GPLVLVKREVVGDDRLYTYLVQFGPKTLRYRVSLIPDGRVSAFRLNEHIP
CCEEEEEHHHCCCCHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]