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The map label for this gene is yhcA [H]

Identifier: 108757178

GI number: 108757178

Start: 1965150

End: 1966604

Strand: Direct

Name: yhcA [H]

Synonym: MXAN_1664

Alternate gene names: 108757178

Gene position: 1965150-1966604 (Clockwise)

Preceding gene: 108761338

Following gene: 108759523

Centisome position: 21.5

GC content: 65.43

Gene sequence:

>1455_bases
ATGCACTCCGCCGCCGAGCCGACAGTCCCCCGCTTCTGGCCCTTCATGTTCACCCTGTTTGTCGGGTCGTTCATGTCGGT
GCTGAGCTCCAGCACGATCACCATCGCCATTCCCGAGCTCCAGCGGCACTTCGGGGCGGAGCTGTCCTCCGTGCAGTGGA
CCCTGACGGGCTTCATGCTCGCCATGGGAACGAGCGCCCCGCTCACTGGCTATCTGGGTGGACGCTTCAGCTTCAAGTGG
GTGTACGTGGCCAGCCTGGTGGCCTTCCTCGTGGCCTCCGTGCTGTGTGGGGTGGCCTGGGACACGCGCTCACTGGTGGC
CTTCCGGTTCGTCCAGGGGGCGTTCTGTGGCGCCATCATGCCGGTGACGATGACGCTCATCTATCAGTTGCTGCCGCGTG
AACGGCAGGCGCTGGCCGCGAGCCTCTGGACCCTGTCCTCCATGCTCGCCCCCGCCTTCGGCCCCACGCTGGCGGGCTGG
CTGATGACGCTGGGCAACTGGCGGTGGTTGTTCTTCATGAACGTGCCGCTGGGCCTCGCCGCCGTGGCGATGGCGGTGCG
GGCCGTTCCCTACTACCGCCTGGAGGTTCCGAAGGCGTTCGACTTCCCCGGGCTGCTCACCGTCATCACCGGAACGCTGG
CGCTGCTGATCGCCTTGAGTCAGGGACGGGCCTGGGGATGGACGGATTCGAAGACGGTGTCGCTGGCCGTGCTGGGGACG
GTGTCACTCGTCTACTTCGTCGTCCGCCAACTGAGGACGCATGCGCCGCTGCTCGACTTGCGGGTGCTGGCCAACGGCCG
CTACGTGGTGACGTTGCTCATCTCGAGCATCATCACCATCAGCCTCTATTCGGGCGCGTTCCTCATCCCGTTGTTCCTCC
AGGAAATCCAGGGTGTGACGCCGCTGAAGACGGGGTTGATTCTTCTCCCGGCCTCCCTGTCCATGGCGCTCCTGATGCCC
CTGGTCGGACGCATGTACGGCAAGCTCGGGCCGAGCACGCTCATGACGGCGGGCGTCCTGTTGATTGCGGGTGGCACATA
TGCCTTGAGCCGCCTGACGCCGGACGTCTCCCACACCTACGTGCTGATGTGGATGCTGGTCCGGAACGTGGGCATCTCGC
TCTCCATCATGCCGGCCAGCAACGCGGGCATGGAGCAGCTCTCCCGGGAGCTGAGTGGCCACGCCTCGTCTATCAGCAAC
TGGCTGCGAAACGTGTTCGGGGCCTTCTCGATTGCCATCTTCACGTCGCTCCTCTCCAGCTTCGCGGCCCGGGAGGCGCA
GGTGCTCGCGCGGGAGGGGATGACGGAGCGTCGTGACATCGAGTTGCTCTCATTCGTGACGGGCATCAACCAGGTGTACC
TGGTGGCGACTGTCATCGCCCTGGTGGCCGTGCCCTTGAGTCTGATGGTCCGGAAGTACGTGAGCGGGCTGGAAGCCTCC
CGGGGCGCTCGCTAG

Upstream 100 bases:

>100_bases
CCGAGGGCCGGCGGCGGCGGGTCCCTTCCGGCTGGACCCTCGGCGTCAGCCGTGATTCATGAGTGGCTTCGGTCTCGCGC
TCCAGCGCCTGCCGCCGCCC

Downstream 100 bases:

>100_bases
CGGGCGCGGCGGCTTTCACGCACCTGGGGCCACCAGTCCCGAGATGTCGCGTGTGATTCGCCCTTCCTGGATGGACGTGT
AGCCGCCCACGGTTCCGGTT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MHSAAEPTVPRFWPFMFTLFVGSFMSVLSSSTITIAIPELQRHFGAELSSVQWTLTGFMLAMGTSAPLTGYLGGRFSFKW
VYVASLVAFLVASVLCGVAWDTRSLVAFRFVQGAFCGAIMPVTMTLIYQLLPRERQALAASLWTLSSMLAPAFGPTLAGW
LMTLGNWRWLFFMNVPLGLAAVAMAVRAVPYYRLEVPKAFDFPGLLTVITGTLALLIALSQGRAWGWTDSKTVSLAVLGT
VSLVYFVVRQLRTHAPLLDLRVLANGRYVVTLLISSIITISLYSGAFLIPLFLQEIQGVTPLKTGLILLPASLSMALLMP
LVGRMYGKLGPSTLMTAGVLLIAGGTYALSRLTPDVSHTYVLMWMLVRNVGISLSIMPASNAGMEQLSRELSGHASSISN
WLRNVFGAFSIAIFTSLLSSFAAREAQVLAREGMTERRDIELLSFVTGINQVYLVATVIALVAVPLSLMVRKYVSGLEAS
RGAR

Sequences:

>Translated_484_residues
MHSAAEPTVPRFWPFMFTLFVGSFMSVLSSSTITIAIPELQRHFGAELSSVQWTLTGFMLAMGTSAPLTGYLGGRFSFKW
VYVASLVAFLVASVLCGVAWDTRSLVAFRFVQGAFCGAIMPVTMTLIYQLLPRERQALAASLWTLSSMLAPAFGPTLAGW
LMTLGNWRWLFFMNVPLGLAAVAMAVRAVPYYRLEVPKAFDFPGLLTVITGTLALLIALSQGRAWGWTDSKTVSLAVLGT
VSLVYFVVRQLRTHAPLLDLRVLANGRYVVTLLISSIITISLYSGAFLIPLFLQEIQGVTPLKTGLILLPASLSMALLMP
LVGRMYGKLGPSTLMTAGVLLIAGGTYALSRLTPDVSHTYVLMWMLVRNVGISLSIMPASNAGMEQLSRELSGHASSISN
WLRNVFGAFSIAIFTSLLSSFAAREAQVLAREGMTERRDIELLSFVTGINQVYLVATVIALVAVPLSLMVRKYVSGLEAS
RGAR
>Mature_484_residues
MHSAAEPTVPRFWPFMFTLFVGSFMSVLSSSTITIAIPELQRHFGAELSSVQWTLTGFMLAMGTSAPLTGYLGGRFSFKW
VYVASLVAFLVASVLCGVAWDTRSLVAFRFVQGAFCGAIMPVTMTLIYQLLPRERQALAASLWTLSSMLAPAFGPTLAGW
LMTLGNWRWLFFMNVPLGLAAVAMAVRAVPYYRLEVPKAFDFPGLLTVITGTLALLIALSQGRAWGWTDSKTVSLAVLGT
VSLVYFVVRQLRTHAPLLDLRVLANGRYVVTLLISSIITISLYSGAFLIPLFLQEIQGVTPLKTGLILLPASLSMALLMP
LVGRMYGKLGPSTLMTAGVLLIAGGTYALSRLTPDVSHTYVLMWMLVRNVGISLSIMPASNAGMEQLSRELSGHASSISN
WLRNVFGAFSIAIFTSLLSSFAAREAQVLAREGMTERRDIELLSFVTGINQVYLVATVIALVAVPLSLMVRKYVSGLEAS
RGAR

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=405, Percent_Identity=28.1481481481481, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1788710, Length=400, Percent_Identity=27.75, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87081983, Length=426, Percent_Identity=25.8215962441315, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1790195, Length=312, Percent_Identity=24.3589743589744, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788392, Length=319, Percent_Identity=23.5109717868339, Blast_Score=85, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323735, Length=318, Percent_Identity=23.5849056603774, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52546; Mature: 52546

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCC
>Mature Secondary Structure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CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]