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The map label for this gene is dnaG

Identifier: 108757060

GI number: 108757060

Start: 6495891

End: 6497711

Strand: Direct

Name: dnaG

Synonym: MXAN_5203

Alternate gene names: 108757060

Gene position: 6495891-6497711 (Clockwise)

Preceding gene: 108759939

Following gene: 108763751

Centisome position: 71.07

GC content: 68.04

Gene sequence:

>1821_bases
GTGGGAGTCGTGATTCCGGAGCACAAGATCCAGGAGGTCCTCGAACGGGTGGACCTCGTCGGGCTCATCTCCCGGCATGT
CGACCTGAAGAAGGCCGGGCGCGAATGGAAGGCCTGCTGTCCCTTCCATCAGGAGAAGACCCCCTCGTTCTACGTGGTGC
CGGAGAAGCGCTTCTATTTCTGCCATGGCTGCCGCGCGAGCGGTGACGCGGTGTCCTTCGTCCAGCGCTACCTGGGCAAG
ACGTTCCTGGATGCGGTGCGGGACCTGGCGCGCGAGCTGGGCGTGGACCTGGAAGCCGAGCAGGACCCCAGCATGCGGGA
GCGGCAGCAAATCAAGGAGGCCACGGACCAGGCCGCCGAGCACTTCCGCGCCATGCTGTGGCAGCAGGACGAAGGCCGCT
CCGCCCGCGCCTACATCGCCAGCCGCGGCGTCTCCGACGAGACGGCCATGGCGTTCGGCCTGGGCTGGGCGCCGCTCGAA
TGGGCCTCACTGACGGAGCGCTTCCAGAAGCTGGGCATGCTGGAGTGGGCGGCGAAGGCCGGCCTGGTCCTCAAGCGCAA
CAGCGGCGACGGCTACTACGACTTCTTCCGCAGCCGAGTGATGGTGCCCATCCGCGCGCCGGAGGGCCGCCCCATCGCCT
TCGGTGGCCGTCTGATTGGCGCGGATGAAGGGCCCAAGTACCTCAACTCGCGCGAGTCCCGGCTCTACAACAAGAGCGAG
ACGCTCTTCGGCATGGACCAGTCTCGCGACGAGATTCGCAAGCGCAAGGCCGCCGTGCTGGTAGAGGGCTACTTCGACGC
CCTTGGCCTCCACCAGGTGGGCGTGCGGCACGCGGTGGCGCTGTGCTCCACCAACCTCACCGCCGGACACATGCAGGTGC
TGAAGCGCGCCGAGGCCCGCGAGCTGATTCTGCTGTTGGACGGAGATTCGGCCGGCCTTGCCGCTGTCGAACGGCTTTCC
GGTCCCCTGCTCGCCGCGGGAGCGACCGCGCGAGTTGCCCTGCTGCCGCAAGGGGATGATCCAGACACCTTCGCGCGTCG
AGAGGGCCAGGAGGGCGTGGAACGGCTGCTGGAAGGCGCCCACCCGCTCACCAGCCACCTGTTCGCCTCGCTCCTCCCGG
AGGGCAAGGCCGCGAGCTTCGAGGAGAAGATGGCAGCGCTCGAGCGGCTCAAGCCGGTGGTGGGGCAGGTCCCCGTGGGC
CTGGTGCGCGCCACCCTCTTCAGCGCGGTGGCCGAGCACTTCGGCTGGCGGCCTGCGGACGTGGAGGCAGCCCTGCGCAG
CAAGGTGCCCCTGCCCAAGCCCGCCGGCGGGGACGCACCTCCTTCGAGCCCCAACCGCCCCGCCCCGCCCCTGGAGAAGC
CGCCCCCCGCGCTGGAGTGCTTCTACGTGGGCGCGGTGTTGAAAGAGCCACGGTTGATGGCGCGGGACACCTTCCGCGTG
TGTGACGAGCTGTCCCACATGGGCCTGCGCATGGCGCTGGCGCATGCGACGTCGGGACACGGCGCAAATGATGCGCTCTT
TGAATCCTCGGAAGCCGTGAAGCGCGGCATTGAAAGCGCGCTGCGGCAGCTCCCCTCGGAACCTGTTCCCCTGGAGGCGG
CGTTCCTCTCCATCTGCAGGGAAATCATGGTGCGGCGCATCGACGAGCGGCTCGTTTATATAAAGCGGGCGACGGAGCAG
ACGCCGGGGGCGTTCGACCTGACAGAGGAGACGCGCCAGCTCCTTGTGGAGCGCGTGGAACTGTTGGCGCTCAAGAAGCG
TGTCCTGGAGGAGCTCAAGCCTGCCTCCTCGGGAACAAAGGCGCCCATGCAACCGGTTTGA

Upstream 100 bases:

>100_bases
CCCCCATGGGGATGATCCGCCAGGTCGGCCGGTGGGCAGGCGACGTCAGCCCTGTCAGGCAGGATGCAGAGGCTGGGCCC
GCCCAGGGGTGGGCCAGGGG

Downstream 100 bases:

>100_bases
GTTCGCGTTTGTAAGAAACCCTGACTTTGCGGTAGATCGGCCGGCTCGCCCAGGCCTCAAGCCCCTGAATTTGCTCAAGG
AGAAGTACCCGAATGCCGAC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MGVVIPEHKIQEVLERVDLVGLISRHVDLKKAGREWKACCPFHQEKTPSFYVVPEKRFYFCHGCRASGDAVSFVQRYLGK
TFLDAVRDLARELGVDLEAEQDPSMRERQQIKEATDQAAEHFRAMLWQQDEGRSARAYIASRGVSDETAMAFGLGWAPLE
WASLTERFQKLGMLEWAAKAGLVLKRNSGDGYYDFFRSRVMVPIRAPEGRPIAFGGRLIGADEGPKYLNSRESRLYNKSE
TLFGMDQSRDEIRKRKAAVLVEGYFDALGLHQVGVRHAVALCSTNLTAGHMQVLKRAEARELILLLDGDSAGLAAVERLS
GPLLAAGATARVALLPQGDDPDTFARREGQEGVERLLEGAHPLTSHLFASLLPEGKAASFEEKMAALERLKPVVGQVPVG
LVRATLFSAVAEHFGWRPADVEAALRSKVPLPKPAGGDAPPSSPNRPAPPLEKPPPALECFYVGAVLKEPRLMARDTFRV
CDELSHMGLRMALAHATSGHGANDALFESSEAVKRGIESALRQLPSEPVPLEAAFLSICREIMVRRIDERLVYIKRATEQ
TPGAFDLTEETRQLLVERVELLALKKRVLEELKPASSGTKAPMQPV

Sequences:

>Translated_606_residues
MGVVIPEHKIQEVLERVDLVGLISRHVDLKKAGREWKACCPFHQEKTPSFYVVPEKRFYFCHGCRASGDAVSFVQRYLGK
TFLDAVRDLARELGVDLEAEQDPSMRERQQIKEATDQAAEHFRAMLWQQDEGRSARAYIASRGVSDETAMAFGLGWAPLE
WASLTERFQKLGMLEWAAKAGLVLKRNSGDGYYDFFRSRVMVPIRAPEGRPIAFGGRLIGADEGPKYLNSRESRLYNKSE
TLFGMDQSRDEIRKRKAAVLVEGYFDALGLHQVGVRHAVALCSTNLTAGHMQVLKRAEARELILLLDGDSAGLAAVERLS
GPLLAAGATARVALLPQGDDPDTFARREGQEGVERLLEGAHPLTSHLFASLLPEGKAASFEEKMAALERLKPVVGQVPVG
LVRATLFSAVAEHFGWRPADVEAALRSKVPLPKPAGGDAPPSSPNRPAPPLEKPPPALECFYVGAVLKEPRLMARDTFRV
CDELSHMGLRMALAHATSGHGANDALFESSEAVKRGIESALRQLPSEPVPLEAAFLSICREIMVRRIDERLVYIKRATEQ
TPGAFDLTEETRQLLVERVELLALKKRVLEELKPASSGTKAPMQPV
>Mature_605_residues
GVVIPEHKIQEVLERVDLVGLISRHVDLKKAGREWKACCPFHQEKTPSFYVVPEKRFYFCHGCRASGDAVSFVQRYLGKT
FLDAVRDLARELGVDLEAEQDPSMRERQQIKEATDQAAEHFRAMLWQQDEGRSARAYIASRGVSDETAMAFGLGWAPLEW
ASLTERFQKLGMLEWAAKAGLVLKRNSGDGYYDFFRSRVMVPIRAPEGRPIAFGGRLIGADEGPKYLNSRESRLYNKSET
LFGMDQSRDEIRKRKAAVLVEGYFDALGLHQVGVRHAVALCSTNLTAGHMQVLKRAEARELILLLDGDSAGLAAVERLSG
PLLAAGATARVALLPQGDDPDTFARREGQEGVERLLEGAHPLTSHLFASLLPEGKAASFEEKMAALERLKPVVGQVPVGL
VRATLFSAVAEHFGWRPADVEAALRSKVPLPKPAGGDAPPSSPNRPAPPLEKPPPALECFYVGAVLKEPRLMARDTFRVC
DELSHMGLRMALAHATSGHGANDALFESSEAVKRGIESALRQLPSEPVPLEAAFLSICREIMVRRIDERLVYIKRATEQT
PGAFDLTEETRQLLVERVELLALKKRVLEELKPASSGTKAPMQPV

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Escherichia coli, GI1789447, Length=481, Percent_Identity=35.7588357588358, Blast_Score=268, Evalue=6e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIM_MYXXA (P50070)

Other databases:

- EMBL:   U20669
- PIR:   S70829
- ProteinModelPortal:   P50070
- SMR:   P50070
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694
- Gene3D:   G3DSA:3.90.980.10
- Gene3D:   G3DSA:3.90.580.10
- SMART:   SM00493
- SMART:   SM00400
- TIGRFAMs:   TIGR01391

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2

EC number: 2.7.7.-

Molecular weight: Translated: 66848; Mature: 66716

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVVIPEHKIQEVLERVDLVGLISRHVDLKKAGREWKACCPFHQEKTPSFYVVPEKRFYF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEECCCEEE
CHGCRASGDAVSFVQRYLGKTFLDAVRDLARELGVDLEAEQDPSMRERQQIKEATDQAAE
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
HFRAMLWQQDEGRSARAYIASRGVSDETAMAFGLGWAPLEWASLTERFQKLGMLEWAAKA
HHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHC
GLVLKRNSGDGYYDFFRSRVMVPIRAPEGRPIAFGGRLIGADEGPKYLNSRESRLYNKSE
CEEEEECCCCCHHHHHHCCEEEEEECCCCCEEEECCEEECCCCCCHHHCCHHHHHHCCCC
TLFGMDQSRDEIRKRKAAVLVEGYFDALGLHQVGVRHAVALCSTNLTAGHMQVLKRAEAR
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
ELILLLDGDSAGLAAVERLSGPLLAAGATARVALLPQGDDPDTFARREGQEGVERLLEGA
EEEEEEECCCCHHHHHHHHCCCEEECCCCEEEEEECCCCCCHHHHHHCCHHHHHHHHHCC
HPLTSHLFASLLPEGKAASFEEKMAALERLKPVVGQVPVGLVRATLFSAVAEHFGWRPAD
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCH
VEAALRSKVPLPKPAGGDAPPSSPNRPAPPLEKPPPALECFYVGAVLKEPRLMARDTFRV
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
CDELSHMGLRMALAHATSGHGANDALFESSEAVKRGIESALRQLPSEPVPLEAAFLSICR
HHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
EIMVRRIDERLVYIKRATEQTPGAFDLTEETRQLLVERVELLALKKRVLEELKPASSGTK
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
APMQPV
CCCCCC
>Mature Secondary Structure 
GVVIPEHKIQEVLERVDLVGLISRHVDLKKAGREWKACCPFHQEKTPSFYVVPEKRFYF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEECCCEEE
CHGCRASGDAVSFVQRYLGKTFLDAVRDLARELGVDLEAEQDPSMRERQQIKEATDQAAE
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
HFRAMLWQQDEGRSARAYIASRGVSDETAMAFGLGWAPLEWASLTERFQKLGMLEWAAKA
HHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHC
GLVLKRNSGDGYYDFFRSRVMVPIRAPEGRPIAFGGRLIGADEGPKYLNSRESRLYNKSE
CEEEEECCCCCHHHHHHCCEEEEEECCCCCEEEECCEEECCCCCCHHHCCHHHHHHCCCC
TLFGMDQSRDEIRKRKAAVLVEGYFDALGLHQVGVRHAVALCSTNLTAGHMQVLKRAEAR
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
ELILLLDGDSAGLAAVERLSGPLLAAGATARVALLPQGDDPDTFARREGQEGVERLLEGA
EEEEEEECCCCHHHHHHHHCCCEEECCCCEEEEEECCCCCCHHHHHHCCHHHHHHHHHCC
HPLTSHLFASLLPEGKAASFEEKMAALERLKPVVGQVPVGLVRATLFSAVAEHFGWRPAD
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCH
VEAALRSKVPLPKPAGGDAPPSSPNRPAPPLEKPPPALECFYVGAVLKEPRLMARDTFRV
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
CDELSHMGLRMALAHATSGHGANDALFESSEAVKRGIESALRQLPSEPVPLEAAFLSICR
HHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
EIMVRRIDERLVYIKRATEQTPGAFDLTEETRQLLVERVELLALKKRVLEELKPASSGTK
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
APMQPV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA