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The map label for this gene is dnaB [H]

Identifier: 108756869

GI number: 108756869

Start: 6356906

End: 6358276

Strand: Direct

Name: dnaB [H]

Synonym: MXAN_5084

Alternate gene names: 108756869

Gene position: 6356906-6358276 (Clockwise)

Preceding gene: 108759263

Following gene: 108758747

Centisome position: 69.55

GC content: 66.96

Gene sequence:

>1371_bases
ATGGAGAACGTCCACGAGTTCAGAGAGGGTCGGAAGGTCCACGAGGACCTCGCCGCGGAGCGCGCCGTGCTGGGCGCCGT
GCTGGCGGACAATACGCTCATCGCCGCGGTAGGCGAGGTCGTCCACCCAGAGGATTTCTCCAGCCCCGCGCACGCCCAGA
TTTTCGAGGCGATGCTGAAGCTCGACGGCCAGTCGAGACAGGTGGACCACCTGACGCTGGCCGAGGAGCTGAAGGTGTTG
GGCCACCTGGTGTCGGTGGGCGGCCCGGCCTACCTGATGCGTCTGGACCAGGTGGTGCCCATCGCGTCCAACGCGGTCCA
GTACGCGCAGATCGTCAAGGACCAGGCCATCCGCCGCCGCCTGGCCCAGGCGGGCAGGGAGATCCAGGACCTCGCCAGCC
AGGAGACGGGCGAGCTGGAGGTGCTGCTCGACGAGGCCGAGCGCAAGGTATTCCTCCTCGCGGAGAAGAAGCGCGAGGGC
GACCTGCGCCCGGTCAGCGAGCTGATGGAGCATACGCTCGACCTGCTCGACAAGATGAAGGCGGCGGCCACGGGCATCAC
GGGCCTGTCCACCGGCTACATCGACCTGGACAACCAGCTCACCGGCCTGCACGCCGGCGAGCTCATCATCCTCGCGGCGC
GTCCCGGCATCGGCAAGACGTCCTTCGCGATGAACATCGCGGTGCACGCGGCGCTGAAGGAGAACAAGGCCGTCGGCATC
TTCAGCCTCGAAATGCCCGCCGACCAGTTGCTGATGCGTCTGCTGGCCTCCACCGCGCGCGTGGACATGAAGAAGCTGCG
CGGCGGCCGGCTGACGCCGCACGACGAGGAGAAGTTCCAGGAGATGGCGGGCGCGCTCTACAACGCCCCCATCTACATCG
ACGACTCCGGCGGTCTGTCCCCGTTCGACCTGCGCGCCAAGGCCCGCCGCGTGAAGCAGCGCGACCCGCGCCTGTCGCTC
ATCGTCATCGACTACCTCCAGCTCATGCATCAGAAGGGCAAGGTGGAGAGCCGCCAGTTGGAGGTCGCCGAAATCTCCCG
CGCACTGAAGCAGCTCGCCAAGGAGCTGGAGGTGCCCATCATCGCGCTCAGTCAGCTCAGCCGAAAGGTGGAGGACCGCA
AGGACGGCAAGCCCCTGCTGTCAGACCTCCGTGAGTCCGGCTCCATCGAGCAGGACGCCGACGTGGTGATGTTCATCCAC
CGTGAGACGTCTGACGAGGCGGGCCCCGACGGGCAGCCGGCGCAGCAGTCGAACACCGTCATCCCCGTGGAGCTCATCAT
CGCCAAGCAGCGTAACGGCCCCATTGGCTCCATCGACCTGGTGTTCCTGGCCGAGTACACCCGCTTCGAGAGCCGGTCGC
GGAGCGAGTAG

Upstream 100 bases:

>100_bases
TCCTCCAGAAGTGGGGACTGGAGGGGTAAGCGTCACTGCTACAGGGCTGTGGCAGCAAATTTGGTTGTGTCAGCCCTGCC
TGTAGAGTGGGCGGGCTGCC

Downstream 100 bases:

>100_bases
CAGCCCTCCGTGTTCGCGCGGGTGCGGTGCACCTCGCACCCGCCCCTGCCGGTTCGCAGGAGCAGCGGGCCCGCCCTACT
CCGCGCCGGTCCGCAGGTAG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MENVHEFREGRKVHEDLAAERAVLGAVLADNTLIAAVGEVVHPEDFSSPAHAQIFEAMLKLDGQSRQVDHLTLAEELKVL
GHLVSVGGPAYLMRLDQVVPIASNAVQYAQIVKDQAIRRRLAQAGREIQDLASQETGELEVLLDEAERKVFLLAEKKREG
DLRPVSELMEHTLDLLDKMKAAATGITGLSTGYIDLDNQLTGLHAGELIILAARPGIGKTSFAMNIAVHAALKENKAVGI
FSLEMPADQLLMRLLASTARVDMKKLRGGRLTPHDEEKFQEMAGALYNAPIYIDDSGGLSPFDLRAKARRVKQRDPRLSL
IVIDYLQLMHQKGKVESRQLEVAEISRALKQLAKELEVPIIALSQLSRKVEDRKDGKPLLSDLRESGSIEQDADVVMFIH
RETSDEAGPDGQPAQQSNTVIPVELIIAKQRNGPIGSIDLVFLAEYTRFESRSRSE

Sequences:

>Translated_456_residues
MENVHEFREGRKVHEDLAAERAVLGAVLADNTLIAAVGEVVHPEDFSSPAHAQIFEAMLKLDGQSRQVDHLTLAEELKVL
GHLVSVGGPAYLMRLDQVVPIASNAVQYAQIVKDQAIRRRLAQAGREIQDLASQETGELEVLLDEAERKVFLLAEKKREG
DLRPVSELMEHTLDLLDKMKAAATGITGLSTGYIDLDNQLTGLHAGELIILAARPGIGKTSFAMNIAVHAALKENKAVGI
FSLEMPADQLLMRLLASTARVDMKKLRGGRLTPHDEEKFQEMAGALYNAPIYIDDSGGLSPFDLRAKARRVKQRDPRLSL
IVIDYLQLMHQKGKVESRQLEVAEISRALKQLAKELEVPIIALSQLSRKVEDRKDGKPLLSDLRESGSIEQDADVVMFIH
RETSDEAGPDGQPAQQSNTVIPVELIIAKQRNGPIGSIDLVFLAEYTRFESRSRSE
>Mature_456_residues
MENVHEFREGRKVHEDLAAERAVLGAVLADNTLIAAVGEVVHPEDFSSPAHAQIFEAMLKLDGQSRQVDHLTLAEELKVL
GHLVSVGGPAYLMRLDQVVPIASNAVQYAQIVKDQAIRRRLAQAGREIQDLASQETGELEVLLDEAERKVFLLAEKKREG
DLRPVSELMEHTLDLLDKMKAAATGITGLSTGYIDLDNQLTGLHAGELIILAARPGIGKTSFAMNIAVHAALKENKAVGI
FSLEMPADQLLMRLLASTARVDMKKLRGGRLTPHDEEKFQEMAGALYNAPIYIDDSGGLSPFDLRAKARRVKQRDPRLSL
IVIDYLQLMHQKGKVESRQLEVAEISRALKQLAKELEVPIIALSQLSRKVEDRKDGKPLLSDLRESGSIEQDADVVMFIH
RETSDEAGPDGQPAQQSNTVIPVELIIAKQRNGPIGSIDLVFLAEYTRFESRSRSE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=448, Percent_Identity=43.75, Blast_Score=341, Evalue=5e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50324; Mature: 50324

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENVHEFREGRKVHEDLAAERAVLGAVLADNTLIAAVGEVVHPEDFSSPAHAQIFEAMLK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LDGQSRQVDHLTLAEELKVLGHLVSVGGPAYLMRLDQVVPIASNAVQYAQIVKDQAIRRR
CCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LAQAGREIQDLASQETGELEVLLDEAERKVFLLAEKKREGDLRPVSELMEHTLDLLDKMK
HHHHCHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
AAATGITGLSTGYIDLDNQLTGLHAGELIILAARPGIGKTSFAMNIAVHAALKENKAVGI
HHHCCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEE
FSLEMPADQLLMRLLASTARVDMKKLRGGRLTPHDEEKFQEMAGALYNAPIYIDDSGGLS
EEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCC
PFDLRAKARRVKQRDPRLSLIVIDYLQLMHQKGKVESRQLEVAEISRALKQLAKELEVPI
CHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCH
IALSQLSRKVEDRKDGKPLLSDLRESGSIEQDADVVMFIHRETSDEAGPDGQPAQQSNTV
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCE
IPVELIIAKQRNGPIGSIDLVFLAEYTRFESRSRSE
EEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MENVHEFREGRKVHEDLAAERAVLGAVLADNTLIAAVGEVVHPEDFSSPAHAQIFEAMLK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LDGQSRQVDHLTLAEELKVLGHLVSVGGPAYLMRLDQVVPIASNAVQYAQIVKDQAIRRR
CCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LAQAGREIQDLASQETGELEVLLDEAERKVFLLAEKKREGDLRPVSELMEHTLDLLDKMK
HHHHCHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
AAATGITGLSTGYIDLDNQLTGLHAGELIILAARPGIGKTSFAMNIAVHAALKENKAVGI
HHHCCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEE
FSLEMPADQLLMRLLASTARVDMKKLRGGRLTPHDEEKFQEMAGALYNAPIYIDDSGGLS
EEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCC
PFDLRAKARRVKQRDPRLSLIVIDYLQLMHQKGKVESRQLEVAEISRALKQLAKELEVPI
CHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCH
IALSQLSRKVEDRKDGKPLLSDLRESGSIEQDADVVMFIHRETSDEAGPDGQPAQQSNTV
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCE
IPVELIIAKQRNGPIGSIDLVFLAEYTRFESRSRSE
EEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]