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The map label for this gene is murE [H]

Identifier: 108563844

GI number: 108563844

Start: 1476180

End: 1477523

Strand: Reverse

Name: murE [H]

Synonym: HPAG1_1419

Alternate gene names: 108563844

Gene position: 1477523-1476180 (Counterclockwise)

Preceding gene: 108563845

Following gene: 108563843

Centisome position: 92.56

GC content: 38.02

Gene sequence:

>1344_bases
ATGAAGCTTAAAAAAACCCTGACTTATCAAAACCACACCTATTCTTTTTTGAGCGATAACACGCATGAAGTTTTAGAAAA
CCCTAAAGAAATCCTTTTTGTCAAAACGCCTTTAAATGAAAAATACTCTCCTTTAATTGCAGAAAAAAACCTGGCTATTT
TAGATTTTAACGAGCTTAAAAACTATTTTGATTTTAAAATGAAAATTGTAGGGATTACTGGCACTAATGGTAAAACGACC
ACAGCGAGTTTGATGTATTCCTTGCTCTTAGATTTGAATAAAAAGACCGCTCTTTTAGGCACAAGAGGGTTTTTTATCAA
CGATGAACGAATCAAAGAAAAGGGCTTGACCACGCCCACTCTTTTAGAGCTTTATAGCGATTTAGAAGAAGCGGTGCGTT
TAAAATGCGAATACTTTATCATGGAAGTGAGCTCCCATGCGATTGTCCAAAAGCGCGTTGCCGGGCTTGATTTCGCCCTT
AAAATCTTAACCAATATCACAAGCGATCATTTAGATTTCCACAAAAGCATAGAAAATTACAGAGACGCTAAAAACAGCTT
TTTTAAAGATGAGGGCTTGAAAGTGATCAACAGAGATGAAACAAACGCCCTTTTTAACCCCATTAACGCGCACACTTACG
CGCTGGATAAAAAAGCGCATTTGAACGTTCAAGCCTTTTCGCTCACCCCCTCTATTAGCGCGTCTTTATGCTACCAACAA
GATTTAAGAGATCCTAATCTCAAAGAAATCGCTCTCATCCATTCCCCCCTTTTAGGGCGTTACAACCTTTATAATATTTT
AGCGGGCGTTTTAGGGGTTAAATTGCTCACTCAATTACCGCTAGAAGTAATCGCGCCGTTATTAGAAAACTTTTATGGGG
TGAAGGGGCGTTTGGAAATTGTGCATTCTAAACCTTTAGTGATCGTGGATTTTGCCCACACCACAGACGGCATGCAACAA
GTCTTTGAAAGCTTTAAAAACCAAAAAATCACCGCTCTTTTTGGAGCAGGGGGCGATAGGGATAAAACCAAGCGCCCTAA
AATGGGAGCCATAGCGAGTTGTTATGCGCATCAAATCGTCTTAACTTCAGACAACCCCAGAAGCGAAAACGAAGAAGACA
TTATTAAGGATATTTTAAAAGGCATCAGCGATTCTTCTAAAGTCATTGTAGAAAAAGACCGAAAAAAAGCCATTTTAAAC
GCTTTAGAAAATTTAAAAGACGATGAGGTGTTATTGATTTTAGGCAAGGGCGATGAAAACACTCAAATCTTTAAAGACAA
AACGATTTTTTTTAGCGACCAAGAAGTCGTTAAAGATTATTATCTCAATTTAAAACAAGGATGA

Upstream 100 bases:

>100_bases
GATCAATTACATTGAAAACATACATGAAGATTCTTTTATTTACGATAAATTTTTGCGTTCTCTTTTAGAAGACGCCCTCA
AATCCAATAAGGCTTAAAGA

Downstream 100 bases:

>100_bases
AACATGCAAGAATTCAGTTTGTGGTGCGATTTTATAGAAAGGGATTTTTTAGAAAACGACTTTTTAAAGCTCATCAATAA
GGGGGCTATTTGTGGGGCGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKLKKTLTYQNHTYSFLSDNTHEVLENPKEILFVKTPLNEKYSPLIAEKNLAILDFNELKNYFDFKMKIVGITGTNGKTT
TASLMYSLLLDLNKKTALLGTRGFFINDERIKEKGLTTPTLLELYSDLEEAVRLKCEYFIMEVSSHAIVQKRVAGLDFAL
KILTNITSDHLDFHKSIENYRDAKNSFFKDEGLKVINRDETNALFNPINAHTYALDKKAHLNVQAFSLTPSISASLCYQQ
DLRDPNLKEIALIHSPLLGRYNLYNILAGVLGVKLLTQLPLEVIAPLLENFYGVKGRLEIVHSKPLVIVDFAHTTDGMQQ
VFESFKNQKITALFGAGGDRDKTKRPKMGAIASCYAHQIVLTSDNPRSENEEDIIKDILKGISDSSKVIVEKDRKKAILN
ALENLKDDEVLLILGKGDENTQIFKDKTIFFSDQEVVKDYYLNLKQG

Sequences:

>Translated_447_residues
MKLKKTLTYQNHTYSFLSDNTHEVLENPKEILFVKTPLNEKYSPLIAEKNLAILDFNELKNYFDFKMKIVGITGTNGKTT
TASLMYSLLLDLNKKTALLGTRGFFINDERIKEKGLTTPTLLELYSDLEEAVRLKCEYFIMEVSSHAIVQKRVAGLDFAL
KILTNITSDHLDFHKSIENYRDAKNSFFKDEGLKVINRDETNALFNPINAHTYALDKKAHLNVQAFSLTPSISASLCYQQ
DLRDPNLKEIALIHSPLLGRYNLYNILAGVLGVKLLTQLPLEVIAPLLENFYGVKGRLEIVHSKPLVIVDFAHTTDGMQQ
VFESFKNQKITALFGAGGDRDKTKRPKMGAIASCYAHQIVLTSDNPRSENEEDIIKDILKGISDSSKVIVEKDRKKAILN
ALENLKDDEVLLILGKGDENTQIFKDKTIFFSDQEVVKDYYLNLKQG
>Mature_447_residues
MKLKKTLTYQNHTYSFLSDNTHEVLENPKEILFVKTPLNEKYSPLIAEKNLAILDFNELKNYFDFKMKIVGITGTNGKTT
TASLMYSLLLDLNKKTALLGTRGFFINDERIKEKGLTTPTLLELYSDLEEAVRLKCEYFIMEVSSHAIVQKRVAGLDFAL
KILTNITSDHLDFHKSIENYRDAKNSFFKDEGLKVINRDETNALFNPINAHTYALDKKAHLNVQAFSLTPSISASLCYQQ
DLRDPNLKEIALIHSPLLGRYNLYNILAGVLGVKLLTQLPLEVIAPLLENFYGVKGRLEIVHSKPLVIVDFAHTTDGMQQ
VFESFKNQKITALFGAGGDRDKTKRPKMGAIASCYAHQIVLTSDNPRSENEEDIIKDILKGISDSSKVIVEKDRKKAILN
ALENLKDDEVLLILGKGDENTQIFKDKTIFFSDQEVVKDYYLNLKQG

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=370, Percent_Identity=32.7027027027027, Blast_Score=168, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 50620; Mature: 50620

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKKTLTYQNHTYSFLSDNTHEVLENPKEILFVKTPLNEKYSPLIAEKNLAILDFNELK
CCCCCEEEECCCEEEECCCCHHHHHCCCCCEEEEECCCCCCCCCEEECCCEEEEEHHHHH
NYFDFKMKIVGITGTNGKTTTASLMYSLLLDLNKKTALLGTRGFFINDERIKEKGLTTPT
HHEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEECHHHHHHCCCCCHH
LLELYSDLEEAVRLKCEYFIMEVSSHAIVQKRVAGLDFALKILTNITSDHLDFHKSIENY
HHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RDAKNSFFKDEGLKVINRDETNALFNPINAHTYALDKKAHLNVQAFSLTPSISASLCYQQ
HHHHHHHHHHCCCEEECCCCCCHHCCCCCCCEEEECCCCCCEEEEEEECCCCHHHHHHHH
DLRDPNLKEIALIHSPLLGRYNLYNILAGVLGVKLLTQLPLEVIAPLLENFYGVKGRLEI
HCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEE
VHSKPLVIVDFAHTTDGMQQVFESFKNQKITALFGAGGDRDKTKRPKMGAIASCYAHQIV
EECCCEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHEEEE
LTSDNPRSENEEDIIKDILKGISDSSKVIVEKDRKKAILNALENLKDDEVLLILGKGDEN
EECCCCCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHCCCCCCEEEEEECCCCC
TQIFKDKTIFFSDQEVVKDYYLNLKQG
CEEEECCEEEECCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLKKTLTYQNHTYSFLSDNTHEVLENPKEILFVKTPLNEKYSPLIAEKNLAILDFNELK
CCCCCEEEECCCEEEECCCCHHHHHCCCCCEEEEECCCCCCCCCEEECCCEEEEEHHHHH
NYFDFKMKIVGITGTNGKTTTASLMYSLLLDLNKKTALLGTRGFFINDERIKEKGLTTPT
HHEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEECHHHHHHCCCCCHH
LLELYSDLEEAVRLKCEYFIMEVSSHAIVQKRVAGLDFALKILTNITSDHLDFHKSIENY
HHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RDAKNSFFKDEGLKVINRDETNALFNPINAHTYALDKKAHLNVQAFSLTPSISASLCYQQ
HHHHHHHHHHCCCEEECCCCCCHHCCCCCCCEEEECCCCCCEEEEEEECCCCHHHHHHHH
DLRDPNLKEIALIHSPLLGRYNLYNILAGVLGVKLLTQLPLEVIAPLLENFYGVKGRLEI
HCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEE
VHSKPLVIVDFAHTTDGMQQVFESFKNQKITALFGAGGDRDKTKRPKMGAIASCYAHQIV
EECCCEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHEEEE
LTSDNPRSENEEDIIKDILKGISDSSKVIVEKDRKKAILNALENLKDDEVLLILGKGDEN
EECCCCCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHCCCCCCEEEEEECCCCC
TQIFKDKTIFFSDQEVVKDYYLNLKQG
CEEEECCEEEECCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]