Click here to switch to the map view.

The map label for this gene is queF [H]

Identifier: 108563764

GI number: 108563764

Start: 1390802

End: 1391248

Strand: Reverse

Name: queF [H]

Synonym: HPAG1_1339

Alternate gene names: 108563764

Gene position: 1391248-1390802 (Counterclockwise)

Preceding gene: 108563769

Following gene: 108563758

Centisome position: 87.15

GC content: 39.6

Gene sequence:

>447_bases
ATGACCCCTGAACTAAACCTCAAATCCTTAGGCGCTAAAACGACCTATATCTTTGAATACAACAGCCAATTATTAGAAGC
TTTCCCTAACCCAAACCCCAATTTAGACCCCTTAATCACGCTAGAGTGCAAGGAATTTACAAGCCTTTGCCCGATCACTT
CTCAGCCGGATTTTGGCGTGATTTTTATCCGCTACATCCCTAAAGATAAAATGGTAGAAAGCAAGTCTTTAAAACTCTAT
TTATTCAGTTACAGAAATCATGGGAGTTTCCATGAGAGTTGCATCAACACGATCTTATTAGATTTAGTCAAATTGCTAGA
GCCAAAGTATTTGGAAGTGTATGGGGATTTTGCCTCTAGGGGCGGGATTGCGATCAAGCCCTTTGTGAATTACGCGATCA
AAGAATACCAGGAATTTAAAGAAAAACGCCTTTTGAATGCGAAATAA

Upstream 100 bases:

>100_bases
TCATCTAATAAAGCCGTAATCGTTTCTATGCGTTTGTTCATGAGTTTCCTAAAAAATGGGTTAAAATAGCCTTATTATAA
CTTAAATCAAGGAAAGATTA

Downstream 100 bases:

>100_bases
CGCGCAAAGATAGGGTTTTAAGATTAAAGCCGTTCCAAATTAAACGAACAAATTAGAGAGAATGACAAGAGAAAAAGAAA
AGAGATTAAAAAAAGAGAGC

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MTPELNLKSLGAKTTYIFEYNSQLLEAFPNPNPNLDPLITLECKEFTSLCPITSQPDFGVIFIRYIPKDKMVESKSLKLY
LFSYRNHGSFHESCINTILLDLVKLLEPKYLEVYGDFASRGGIAIKPFVNYAIKEYQEFKEKRLLNAK

Sequences:

>Translated_148_residues
MTPELNLKSLGAKTTYIFEYNSQLLEAFPNPNPNLDPLITLECKEFTSLCPITSQPDFGVIFIRYIPKDKMVESKSLKLY
LFSYRNHGSFHESCINTILLDLVKLLEPKYLEVYGDFASRGGIAIKPFVNYAIKEYQEFKEKRLLNAK
>Mature_147_residues
TPELNLKSLGAKTTYIFEYNSQLLEAFPNPNPNLDPLITLECKEFTSLCPITSQPDFGVIFIRYIPKDKMVESKSLKLYL
FSYRNHGSFHESCINTILLDLVKLLEPKYLEVYGDFASRGGIAIKPFVNYAIKEYQEFKEKRLLNAK

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=89, Percent_Identity=38.2022471910112, Blast_Score=74, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016856
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 17082; Mature: 16951

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPELNLKSLGAKTTYIFEYNSQLLEAFPNPNPNLDPLITLECKEFTSLCPITSQPDFGV
CCCCCCHHHCCCCEEEEEECCHHHHHHCCCCCCCCCCEEEEEHHHHHHHCCCCCCCCCCE
IFIRYIPKDKMVESKSLKLYLFSYRNHGSFHESCINTILLDLVKLLEPKYLEVYGDFASR
EEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHC
GGIAIKPFVNYAIKEYQEFKEKRLLNAK
CCEEEHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TPELNLKSLGAKTTYIFEYNSQLLEAFPNPNPNLDPLITLECKEFTSLCPITSQPDFGV
CCCCCHHHCCCCEEEEEECCHHHHHHCCCCCCCCCCEEEEEHHHHHHHCCCCCCCCCCE
IFIRYIPKDKMVESKSLKLYLFSYRNHGSFHESCINTILLDLVKLLEPKYLEVYGDFASR
EEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHC
GGIAIKPFVNYAIKEYQEFKEKRLLNAK
CCEEEHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA