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The map label for this gene is phoB [H]

Identifier: 108563737

GI number: 108563737

Start: 1359091

End: 1359732

Strand: Reverse

Name: phoB [H]

Synonym: HPAG1_1312

Alternate gene names: 108563737

Gene position: 1359732-1359091 (Counterclockwise)

Preceding gene: 108563742

Following gene: 108563736

Centisome position: 85.18

GC content: 38.16

Gene sequence:

>642_bases
ATGCAAAAAAAGATTTTTTTACTAGAAGACGATTACCTTTTAAGCGAGAGTATCAAGGAGTTTTTAGAGCATTTGGGCTA
TGAAGTGTTTTGCGTTTTTAACGGGAAAGAGGCTTATGAAAGGCTCTCTGTTGAGCGCTTCAACCTCTTGCTTTTAGACG
TGCAAGTGCCTGAAATGAATAGCTTGGAATTGTTTAAGCGCATCAAAAACGATTTTTTAATCTCTACGCCTGTGATTTTT
ATCACCGCCTTACAGGATAACGCTACCTTAAAAAACGCTTTTAATTTGGGAGCGAGCGATTATTTGAAAAAACCTTTTGA
TTTAGACGAATTAGAAGCGCGCATTAAAAGGTTTTTCAATGATGATCCCATAGAAATCATGCCTAACATTTTTTACCGCC
AAAATTGTTTGAGTGTCAGGGGCAAAAAAGAGATCTTGCCTCCCAAAACCGCCCAACTTTTAGAATACTTTTTAGAGCAT
AAGGGGCAAATCATTAGCTCTCAAGCGTTAGAAAACAATTTATGGGAACAAGCGATTGATGATTCCACTTTACGCACTTA
CATTAAGGTGTTGCGCAAGCTTTTAGGTAAAAATTGCATAGAAACGCATAAGGGGGTGGGCTATCGCTTTAACCCACTAT
GA

Upstream 100 bases:

>100_bases
TTCATAAAATTGCGCTTCGTTCGTTTTCATCATCACGCTCCTTAAAAAACATGAAAAAGTATTATAACAAAGCCCGCTCA
TTAAGCGCTACAATACCCCC

Downstream 100 bases:

>100_bases
AAAAAAATCCCTCAAACTCTTTTTAGGGACTTATTTAGGCTCTTCGTTTGTGTTGATGCTAGTGATTAGCGTTTTAGCGT
TTAACTATGAAAAAAACGAA

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MQKKIFLLEDDYLLSESIKEFLEHLGYEVFCVFNGKEAYERLSVERFNLLLLDVQVPEMNSLELFKRIKNDFLISTPVIF
ITALQDNATLKNAFNLGASDYLKKPFDLDELEARIKRFFNDDPIEIMPNIFYRQNCLSVRGKKEILPPKTAQLLEYFLEH
KGQIISSQALENNLWEQAIDDSTLRTYIKVLRKLLGKNCIETHKGVGYRFNPL

Sequences:

>Translated_213_residues
MQKKIFLLEDDYLLSESIKEFLEHLGYEVFCVFNGKEAYERLSVERFNLLLLDVQVPEMNSLELFKRIKNDFLISTPVIF
ITALQDNATLKNAFNLGASDYLKKPFDLDELEARIKRFFNDDPIEIMPNIFYRQNCLSVRGKKEILPPKTAQLLEYFLEH
KGQIISSQALENNLWEQAIDDSTLRTYIKVLRKLLGKNCIETHKGVGYRFNPL
>Mature_213_residues
MQKKIFLLEDDYLLSESIKEFLEHLGYEVFCVFNGKEAYERLSVERFNLLLLDVQVPEMNSLELFKRIKNDFLISTPVIF
ITALQDNATLKNAFNLGASDYLKKPFDLDELEARIKRFFNDDPIEIMPNIFYRQNCLSVRGKKEILPPKTAQLLEYFLEH
KGQIISSQALENNLWEQAIDDSTLRTYIKVLRKLLGKNCIETHKGVGYRFNPL

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789402, Length=218, Percent_Identity=27.0642201834862, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1790552, Length=217, Percent_Identity=32.258064516129, Blast_Score=89, Evalue=1e-19,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=25.9090909090909, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1786599, Length=226, Percent_Identity=26.9911504424779, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786911, Length=222, Percent_Identity=25.6756756756757, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1790860, Length=237, Percent_Identity=27.0042194092827, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=26.457399103139, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1790299, Length=121, Percent_Identity=34.7107438016529, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787229, Length=225, Percent_Identity=24, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789809, Length=236, Percent_Identity=25, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1788394, Length=227, Percent_Identity=25.1101321585903, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=30.7017543859649, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790437, Length=115, Percent_Identity=32.1739130434783, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1790863, Length=234, Percent_Identity=23.9316239316239, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI2367329, Length=231, Percent_Identity=29.4372294372294, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1787375, Length=221, Percent_Identity=23.9819004524887, Blast_Score=60, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=121, Percent_Identity=28.099173553719, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 24933; Mature: 24933

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKIFLLEDDYLLSESIKEFLEHLGYEVFCVFNGKEAYERLSVERFNLLLLDVQVPEMN
CCCEEEEEECCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHEEEEEEEECCCCC
SLELFKRIKNDFLISTPVIFITALQDNATLKNAFNLGASDYLKKPFDLDELEARIKRFFN
HHHHHHHHHHCEEEECCEEEEEEECCCCHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHCC
DDPIEIMPNIFYRQNCLSVRGKKEILPPKTAQLLEYFLEHKGQIISSQALENNLWEQAID
CCCHHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
DSTLRTYIKVLRKLLGKNCIETHKGVGYRFNPL
HHHHHHHHHHHHHHHCCHHHHHHCCCCEECCCC
>Mature Secondary Structure
MQKKIFLLEDDYLLSESIKEFLEHLGYEVFCVFNGKEAYERLSVERFNLLLLDVQVPEMN
CCCEEEEEECCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHEEEEEEEECCCCC
SLELFKRIKNDFLISTPVIFITALQDNATLKNAFNLGASDYLKKPFDLDELEARIKRFFN
HHHHHHHHHHCEEEECCEEEEEEECCCCHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHCC
DDPIEIMPNIFYRQNCLSVRGKKEILPPKTAQLLEYFLEHKGQIISSQALENNLWEQAID
CCCHHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
DSTLRTYIKVLRKLLGKNCIETHKGVGYRFNPL
HHHHHHHHHHHHHHHCCHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2115874; 10984043 [H]