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The map label for this gene is tenA [H]

Identifier: 108563651

GI number: 108563651

Start: 1288422

End: 1289075

Strand: Direct

Name: tenA [H]

Synonym: HPAG1_1226

Alternate gene names: 108563651

Gene position: 1288422-1289075 (Clockwise)

Preceding gene: 108563646

Following gene: 108563653

Centisome position: 80.71

GC content: 39.14

Gene sequence:

>654_bases
ATGCAAGTTTCACAATATCTGTATCAAAACGCGCAATCTATTTGGGGGGATTGCATTTCCCATCCGTTTGTTCAAGGCAT
AGGGCGTGGGACTTTAGAAAGAGATAAATTCCGTTTTTATATTGTTCAGGATTATCTGTTTCTTTTAGAATACGCTAAGG
TGTTTGCTTTAGGCGTGGTTAAGGCTTGTGATGAAGCGGTGATGAGAGAGTTTTCTAACGCTATACAAGATATTTTGAAT
AACGAGATGAGTATCCATAACCATTACATTAGAGAACTTCAAATCACTCCAATGGAATTGCAAAACGCGTGCCCCACTCT
AGCGAATAAATCCTATACAAGCTACATGCTCGCTGAAGGAATTAAGGGCTCTATCAAAGAAATTACGGTGGCTGTTCTGG
CTTGCGCTTGGAGCTATTTAGTGATCGCGCAAAATTTGAGCCAAATCCCCAACGCTTTAGAACATGCCTTTTATGGGCAT
TGGATTAAGGGCTATAGCTCAAAAGAATTTCAAGCGTGTGTGTCGTGGAATATTAATTTGCTTGATTCTCTCACTCACGC
TTCTTCAAAACAAGAAATTGAGAAATTAAAGGACATTTTTATCACTACAAGCGAATACGAATATCTGTTTTGGGATATGG
CGTATCAAAGTTGA

Upstream 100 bases:

>100_bases
AAGCAAGAGCCCACTCACAAATCTTTTTAACAACAAATTTTTATACCATGTTGATAGCTTTCGTGTTAGAATGCGTTGAA
TGGATTTTAGGAGTGTAAAA

Downstream 100 bases:

>100_bases
ATAAAGGTTTTTGTGCCGTTAGAAAAACGCAAGAGAAGAAGATATCTATCTCTTGCAAAACGATTATTGAGCGTAAGCTT
CTAGTTTGAGCTCAATTTTT

Product: putative transcriptional regulator

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MQVSQYLYQNAQSIWGDCISHPFVQGIGRGTLERDKFRFYIVQDYLFLLEYAKVFALGVVKACDEAVMREFSNAIQDILN
NEMSIHNHYIRELQITPMELQNACPTLANKSYTSYMLAEGIKGSIKEITVAVLACAWSYLVIAQNLSQIPNALEHAFYGH
WIKGYSSKEFQACVSWNINLLDSLTHASSKQEIEKLKDIFITTSEYEYLFWDMAYQS

Sequences:

>Translated_217_residues
MQVSQYLYQNAQSIWGDCISHPFVQGIGRGTLERDKFRFYIVQDYLFLLEYAKVFALGVVKACDEAVMREFSNAIQDILN
NEMSIHNHYIRELQITPMELQNACPTLANKSYTSYMLAEGIKGSIKEITVAVLACAWSYLVIAQNLSQIPNALEHAFYGH
WIKGYSSKEFQACVSWNINLLDSLTHASSKQEIEKLKDIFITTSEYEYLFWDMAYQS
>Mature_217_residues
MQVSQYLYQNAQSIWGDCISHPFVQGIGRGTLERDKFRFYIVQDYLFLLEYAKVFALGVVKACDEAVMREFSNAIQDILN
NEMSIHNHYIRELQITPMELQNACPTLANKSYTSYMLAEGIKGSIKEITVAVLACAWSYLVIAQNLSQIPNALEHAFYGH
WIKGYSSKEFQACVSWNINLLDSLTHASSKQEIEKLKDIFITTSEYEYLFWDMAYQS

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=205, Percent_Identity=26.8292682926829, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6325378, Length=205, Percent_Identity=27.8048780487805, Blast_Score=79, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324997, Length=205, Percent_Identity=26.8292682926829, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 24990; Mature: 24990

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSQYLYQNAQSIWGDCISHPFVQGIGRGTLERDKFRFYIVQDYLFLLEYAKVFALGVV
CCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KACDEAVMREFSNAIQDILNNEMSIHNHYIRELQITPMELQNACPTLANKSYTSYMLAEG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCHHCCCCHHHHHHHHC
IKGSIKEITVAVLACAWSYLVIAQNLSQIPNALEHAFYGHWIKGYSSKEFQACVSWNINL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHH
LDSLTHASSKQEIEKLKDIFITTSEYEYLFWDMAYQS
HHHHHHCCCHHHHHHHHHHHHCCCCCHHHEEEHHHCC
>Mature Secondary Structure
MQVSQYLYQNAQSIWGDCISHPFVQGIGRGTLERDKFRFYIVQDYLFLLEYAKVFALGVV
CCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KACDEAVMREFSNAIQDILNNEMSIHNHYIRELQITPMELQNACPTLANKSYTSYMLAEG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCHHCCCCHHHHHHHHC
IKGSIKEITVAVLACAWSYLVIAQNLSQIPNALEHAFYGHWIKGYSSKEFQACVSWNINL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHH
LDSLTHASSKQEIEKLKDIFITTSEYEYLFWDMAYQS
HHHHHHCCCHHHHHHHHHHHHCCCCCHHHEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]