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The map label for this gene is trmB [H]

Identifier: 108563366

GI number: 108563366

Start: 981970

End: 983151

Strand: Reverse

Name: trmB [H]

Synonym: HPAG1_0941

Alternate gene names: 108563366

Gene position: 983151-981970 (Counterclockwise)

Preceding gene: 108563367

Following gene: 108563365

Centisome position: 61.59

GC content: 39.0

Gene sequence:

>1182_bases
TTGTTTAAGTTTTTCTACCTTTTATTGTTAACTTTAGGGCATCTTCTTGGTGCGCCTTTCATCTTTTTTTGGAGTTTTAA
GGAAAAATACCGCCATTCTTTAAAAGCCCGTTTTTTTCTCAAAGACAATTTTTTAAAAAGCGAGCCGGTTTTTTGGTTCC
ATGCATGCTCTTATGGGGAGGTCAAATCCTTAGAGCCGATTATCCAAGCTTTAAAAGAGCCGATTCTAATCAGCGTTACC
ACTAATACCGGTTTTGAATTAGCCGCTCAAACTTATCAGCATTCTCAACACATAGAAGTGCGTTACCTGCCTTTTGAAAC
CCTATTATTCGCATGGGAAAAAAACTTAAAACGCTTGAAAACTTTGGTGGTTACAGAAGCGGAATTGTGGTTTAATGTGT
TTGATACGGCTCAAAAATTAGGGGCAAAAACCATGCTCATTAACGCTCGTATCAGCGTTCGTTCTTACCCCAAGTATCAG
CGTTTTTCTTTCTTTTATGCGCTTTTATTCAAACGCATTGATTTGATTTTGGCGCAAAGCAAGGAGGATAAAAAGCGCTT
GTTGAATCTAGGGGCTAAAAAAGTGGTGGATTTTTTGAATATCAAGCGTTTTTCAAAGCCTGTGATCACTTCGTTTTACC
CTAAAAACCCAAGCGCTTTAAACATTGTTTTAGCCAGCACGCATGAGGGCGAAGAGGAATTGGGGTTAAAAGCGTTTTTG
GAGTTCAAAAAGACTCATAAGAACGCAAGACTGATTGTTGTGCCGCGCCACCCCGAGCGTTTTAAAAGCGTGCAAAATTT
GTTGCAAGATATTTTAAAAACGACGCCTTTTAGTTGGGAGTGTTTTTCTTCAAAAGGTTTTGTGGAATGCGATATTTTGT
TAGTGGATAGCTTGGGGGAATTGAATAACTTCTACAAAATCGCAGACATTGTCATTTTAGGGGGTTCGTTTGTCAAAATG
GGGGGGCATAACCCTTTAGAGCCGGCGTTTTTTAATGCGCGCTTGATCACAGGGGAGTATCTTTTCAACCAGGTAGCGTT
GTTTGAATTAGTCAAGCCTTATAAGATTGTTCCAAAAGAGGATCTGTTGGATGCTCTTTTAGATTACAAAAATTTAGGCG
TGGCGCGTTTTTTAGAAAACAAGCATGATTTAAACGAATTGCTCGCTTTCATTAAACATTAA

Upstream 100 bases:

>100_bases
TTGCCCGTATTGCGGGCGTATTTTATACGCTGAGGGCGCGTATGAAAGTAACGCTCAACCTCCAAAAGAAAGCCAAGAAG
AAAGCCAAGAATCAGTTTGA

Downstream 100 bases:

>100_bases
AAAGGATAAAGGATAAACATGGAAAAAGCCTACAAATTATTGAGCGTTCAAGAAAACATTTCGCATAAAAAAGCCAAAGC
TTTGATTGATTTGGGGTTAG

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KdtA protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MFKFFYLLLLTLGHLLGAPFIFFWSFKEKYRHSLKARFFLKDNFLKSEPVFWFHACSYGEVKSLEPIIQALKEPILISVT
TNTGFELAAQTYQHSQHIEVRYLPFETLLFAWEKNLKRLKTLVVTEAELWFNVFDTAQKLGAKTMLINARISVRSYPKYQ
RFSFFYALLFKRIDLILAQSKEDKKRLLNLGAKKVVDFLNIKRFSKPVITSFYPKNPSALNIVLASTHEGEEELGLKAFL
EFKKTHKNARLIVVPRHPERFKSVQNLLQDILKTTPFSWECFSSKGFVECDILLVDSLGELNNFYKIADIVILGGSFVKM
GGHNPLEPAFFNARLITGEYLFNQVALFELVKPYKIVPKEDLLDALLDYKNLGVARFLENKHDLNELLAFIKH

Sequences:

>Translated_393_residues
MFKFFYLLLLTLGHLLGAPFIFFWSFKEKYRHSLKARFFLKDNFLKSEPVFWFHACSYGEVKSLEPIIQALKEPILISVT
TNTGFELAAQTYQHSQHIEVRYLPFETLLFAWEKNLKRLKTLVVTEAELWFNVFDTAQKLGAKTMLINARISVRSYPKYQ
RFSFFYALLFKRIDLILAQSKEDKKRLLNLGAKKVVDFLNIKRFSKPVITSFYPKNPSALNIVLASTHEGEEELGLKAFL
EFKKTHKNARLIVVPRHPERFKSVQNLLQDILKTTPFSWECFSSKGFVECDILLVDSLGELNNFYKIADIVILGGSFVKM
GGHNPLEPAFFNARLITGEYLFNQVALFELVKPYKIVPKEDLLDALLDYKNLGVARFLENKHDLNELLAFIKH
>Mature_393_residues
MFKFFYLLLLTLGHLLGAPFIFFWSFKEKYRHSLKARFFLKDNFLKSEPVFWFHACSYGEVKSLEPIIQALKEPILISVT
TNTGFELAAQTYQHSQHIEVRYLPFETLLFAWEKNLKRLKTLVVTEAELWFNVFDTAQKLGAKTMLINARISVRSYPKYQ
RFSFFYALLFKRIDLILAQSKEDKKRLLNLGAKKVVDFLNIKRFSKPVITSFYPKNPSALNIVLASTHEGEEELGLKAFL
EFKKTHKNARLIVVPRHPERFKSVQNLLQDILKTTPFSWECFSSKGFVECDILLVDSLGELNNFYKIADIVILGGSFVKM
GGHNPLEPAFFNARLITGEYLFNQVALFELVKPYKIVPKEDLLDALLDYKNLGVARFLENKHDLNELLAFIKH

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Inner Membrane-Anchored [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=365, Percent_Identity=32.0547945205479, Blast_Score=144, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007507
- InterPro:   IPR003358 [H]

Pfam domain/function: PF04413 Glycos_transf_N; PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 45656; Mature: 45656

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKFFYLLLLTLGHLLGAPFIFFWSFKEKYRHSLKARFFLKDNFLKSEPVFWFHACSYGE
CHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCC
VKSLEPIIQALKEPILISVTTNTGFELAAQTYQHSQHIEVRYLPFETLLFAWEKNLKRLK
CHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHH
TLVVTEAELWFNVFDTAQKLGAKTMLINARISVRSYPKYQRFSFFYALLFKRIDLILAQS
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
KEDKKRLLNLGAKKVVDFLNIKRFSKPVITSFYPKNPSALNIVLASTHEGEEELGLKAFL
CHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCCCHHHHHHHHHH
EFKKTHKNARLIVVPRHPERFKSVQNLLQDILKTTPFSWECFSSKGFVECDILLVDSLGE
HHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEEECCHHH
LNNFYKIADIVILGGSFVKMGGHNPLEPAFFNARLITGEYLFNQVALFELVKPYKIVPKE
HHHHHEEEHEEEECCCEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHH
DLLDALLDYKNLGVARFLENKHDLNELLAFIKH
HHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MFKFFYLLLLTLGHLLGAPFIFFWSFKEKYRHSLKARFFLKDNFLKSEPVFWFHACSYGE
CHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCC
VKSLEPIIQALKEPILISVTTNTGFELAAQTYQHSQHIEVRYLPFETLLFAWEKNLKRLK
CHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHH
TLVVTEAELWFNVFDTAQKLGAKTMLINARISVRSYPKYQRFSFFYALLFKRIDLILAQS
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
KEDKKRLLNLGAKKVVDFLNIKRFSKPVITSFYPKNPSALNIVLASTHEGEEELGLKAFL
CHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCCCHHHHHHHHHH
EFKKTHKNARLIVVPRHPERFKSVQNLLQDILKTTPFSWECFSSKGFVECDILLVDSLGE
HHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEEECCHHH
LNNFYKIADIVILGGSFVKMGGHNPLEPAFFNARLITGEYLFNQVALFELVKPYKIVPKE
HHHHHEEEHEEEECCCEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHH
DLLDALLDYKNLGVARFLENKHDLNELLAFIKH
HHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11889109 [H]