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The map label for this gene is proP [H]

Identifier: 108563342

GI number: 108563342

Start: 962417

End: 963586

Strand: Reverse

Name: proP [H]

Synonym: HPAG1_0917

Alternate gene names: 108563342

Gene position: 963586-962417 (Counterclockwise)

Preceding gene: 108563343

Following gene: 108563330

Centisome position: 60.36

GC content: 39.15

Gene sequence:

>1170_bases
GTGATGGCCCACTTTGGGGATAGATTCGGCCGTAAAAACATGTTCATGCTCTCTATTTTATTGATGGTAATCCCAACCTT
TGCGCTCGCTTTGATGCCAACTTTTAATCATTTTGTGAGTTTTGGCGTTGATAGCATGGGACTTAGCCTAAAAAACGCTC
ATTATCTTGGTTACATAGCTCCTGTTTTTTTGGTGTTTGTTAGGATCTGTCAAGGCATTGCTGTGGGTGGTGAGTTGCCT
GGCGCTTGGGTTTTTGTCCATGAACATGCTCCGCAAGGCCAAAAAAACACTTATATTGGTTTTTTAAGCGCTTCTGTAGT
TTCTGGGATTTTGCTTGGGAGTTTGGTTTATATTGGGATTTATATGGTTTTTGACAAGCCTGTTGTTGAAGATTGGGCTT
GGAGGGTTGCTTTTGGGCTTGGCGGGATTTTTGGCATCATTTCTGTCTATTTGAGGCGCTTTTTAGAAGAAACTCCTGTT
TTTCAGCAAATGAAGCAGGACGATGCCTTAGTCAAATTCCCGCTTAAAGAGGTGTTTAAAAACTCTCTCTTTGGTATATC
AATCTCCATGCTTATCACTTGGGTTTTAACCGCTTGTATTTTGATTTTTATCCTCTTTGTTCCCAATTTTACCCTTACGC
ATCCTAATTTTAATTTCACTCCTTTTGAAAAAACCTATTTTCAAATTTTAGGACTTGTTGGTATTGTAAGTTCTATTATT
TTAACTGGGTTTTTGGCCGATAAAATCAAACCGCACAAAGTTTGTATGGCTTTTAGCGTGGCCTTTGCCTTTTTTGGCTT
TTTATTCTTCAAAGAATTTTATTCTAACGCGCCAAGTTTAGTCAATACCATAGTTTTATACTTTTTAGCGTGCTTTTGCG
CGGGTATTATGAATTTTTGCCCCATTTTTATGAGCGATGTGTTTAGCGCCAAAATCCGTTTTAGCGGGATTTCCTTCGCT
TATAACATAGCCTATGCTATAACCGCTGGCTTTACCCCTCAACTTTCAAGCTGGTTGAATGCAAAAGCCATAGCGGCACC
TGAAAGCTTGCAAAGTTATGGTTTAAGCTTTTATATTTTTGTGGTTGCTTCAATTGCTTTTATCGTATCGCTTTTAATGG
CGCCAATTTACAACCAATCTAACCCCCAACACGAATCGCCCATAACATGA

Upstream 100 bases:

>100_bases
CAAACACTTTTTCCCAAACACGCTCAGCCCCATTTGGTCTGAAATCAACACTTATGGTATCTTTGCTGCAGGCTATCTAG
CGCGCCCGCTTGGCGGCATA

Downstream 100 bases:

>100_bases
AAGAAGTGCAAACCTTTGAGTTATTCAATCAAAACTTAGCCGATAGCGTTGGTGGGTTTTGGTGATGATGAAATCCTTAA
TATTCAAAGATTTTTAAACT

Product: proline and betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MMAHFGDRFGRKNMFMLSILLMVIPTFALALMPTFNHFVSFGVDSMGLSLKNAHYLGYIAPVFLVFVRICQGIAVGGELP
GAWVFVHEHAPQGQKNTYIGFLSASVVSGILLGSLVYIGIYMVFDKPVVEDWAWRVAFGLGGIFGIISVYLRRFLEETPV
FQQMKQDDALVKFPLKEVFKNSLFGISISMLITWVLTACILIFILFVPNFTLTHPNFNFTPFEKTYFQILGLVGIVSSII
LTGFLADKIKPHKVCMAFSVAFAFFGFLFFKEFYSNAPSLVNTIVLYFLACFCAGIMNFCPIFMSDVFSAKIRFSGISFA
YNIAYAITAGFTPQLSSWLNAKAIAAPESLQSYGLSFYIFVVASIAFIVSLLMAPIYNQSNPQHESPIT

Sequences:

>Translated_389_residues
MMAHFGDRFGRKNMFMLSILLMVIPTFALALMPTFNHFVSFGVDSMGLSLKNAHYLGYIAPVFLVFVRICQGIAVGGELP
GAWVFVHEHAPQGQKNTYIGFLSASVVSGILLGSLVYIGIYMVFDKPVVEDWAWRVAFGLGGIFGIISVYLRRFLEETPV
FQQMKQDDALVKFPLKEVFKNSLFGISISMLITWVLTACILIFILFVPNFTLTHPNFNFTPFEKTYFQILGLVGIVSSII
LTGFLADKIKPHKVCMAFSVAFAFFGFLFFKEFYSNAPSLVNTIVLYFLACFCAGIMNFCPIFMSDVFSAKIRFSGISFA
YNIAYAITAGFTPQLSSWLNAKAIAAPESLQSYGLSFYIFVVASIAFIVSLLMAPIYNQSNPQHESPIT
>Mature_389_residues
MMAHFGDRFGRKNMFMLSILLMVIPTFALALMPTFNHFVSFGVDSMGLSLKNAHYLGYIAPVFLVFVRICQGIAVGGELP
GAWVFVHEHAPQGQKNTYIGFLSASVVSGILLGSLVYIGIYMVFDKPVVEDWAWRVAFGLGGIFGIISVYLRRFLEETPV
FQQMKQDDALVKFPLKEVFKNSLFGISISMLITWVLTACILIFILFVPNFTLTHPNFNFTPFEKTYFQILGLVGIVSSII
LTGFLADKIKPHKVCMAFSVAFAFFGFLFFKEFYSNAPSLVNTIVLYFLACFCAGIMNFCPIFMSDVFSAKIRFSGISFA
YNIAYAITAGFTPQLSSWLNAKAIAAPESLQSYGLSFYIFVVASIAFIVSLLMAPIYNQSNPQHESPIT

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=389, Percent_Identity=27.7634961439589, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1788292, Length=338, Percent_Identity=27.810650887574, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1789941, Length=388, Percent_Identity=24.7422680412371, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788942, Length=394, Percent_Identity=24.8730964467005, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 43354; Mature: 43354

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]