Click here to switch to the map view.

The map label for this gene is hsdM [H]

Identifier: 108563258

GI number: 108563258

Start: 869298

End: 870821

Strand: Reverse

Name: hsdM [H]

Synonym: HPAG1_0833

Alternate gene names: 108563258

Gene position: 870821-869298 (Counterclockwise)

Preceding gene: 108563260

Following gene: 108563257

Centisome position: 54.55

GC content: 41.01

Gene sequence:

>1524_bases
TTGCACAACACCATTTGGAAAGTGGCGAACGAATTGAGAGGCTCAGTGGATGGCTGGGATTTTAAGCAATACGTTTTAGG
CATTCTTTTTTACCGCTACATTTCAGAAAACATGACACATTACATCAATAAAAAAGAGCGAAAGCGCGATCCGGATTTTG
ATTACGCCAAATTAAGCGATGAAGAGGCCAAGCACGCAAGAAAACACCTTATTGAAGAAAAAGACTTTTTCATCCCGCCA
AGCGCTTTATTTTGTAATGCGCTAAAAAACGCGCCCCATAACGAAGATCTCAATGTAACCCTACAAAATATTTTTAACGA
AATAGAAAAATCCAGCCTTGGCACTCCATCAGAAGAAAATGTCAAAGGCTTGTTTGCGGATTTAGACGTCAATAGCAATA
AATTAGGGAGCTCTCATAAAAATAGGGTGGAAAAATTGACTAAAATCCTTGAAGCCATAGGGGGCATGCAATTAGGCGAT
TATTTAAAAAGCGGGATTGATGTTTTTGGCGACGCTTATGAATATTTAATGGCCATGTATGCGAGCAATGCCGGCAAAAG
CGGAGGGGAATTTTTCACCCCCCAAGAAGTGAGCGAACTGCTCGCTAAAATCACCCTGCACAACCAAGAGAGCGTCAATA
AAGTTTATGACCCATGCTGTGGGAGCGGAAGCTTACTCTTACAATTTTCTAAAGTGTTAGGCGATAAAAACGTTTCAAAA
GGGTATTTTGGGCAAGAAATCAACTTGACCACTTACAACCTTTGCCGCATCAACATGTTTTTGCATGACATCAATTACAC
TAAATTCCACATTGCACTAGGGGACACGCTTTTAGACCCAAAACATGAAGATGATGAGCCTTTTGATGCAATCGTTTCTA
ACCCTCCTTATTCCACTAAATGGGGGGGCGATAACAACCCCCTTTTAATCAATGATGAGCGCTTTAGCCCGGCCGGTGTG
TTAGCGCCCAAAAACGCCGCCGATCTCGCATTCACTATGCACATGCTTTCTTATTTATCCAATAGCGGCACTGCTGCGAT
CGTGGAATTTCCTGGGGTGCTTTATAGGGGAAATGCTGAAGCAAAAATCAGAGAATATTTAGTCAAAGAGAATGTCATTG
ACTGCGTGATCGCTTTACCAGACAACCTCTTTTTTGGAACGAATATCGCTACTTGCATTTTAGTGCTTAAGAAAAATAAA
AAAGACGACACCACGCTTTTTATTGATGCGAGTAAGGAATTTGTCAAAGAAGGCAAGAAAAACAAGCTCAAAGCGCATAA
CCGGGAAAAGATTTTGCAAACCTATATTGAAAGAAAAGAGGTTAAGCACTTTTGCGCCCTAGCCAATATGGAGCAAATCC
AAGAAAACGATTACAACCTGTCTGTGAACCGCTATGTGGAGCAAGAAGACACGAAAGAAGCCATTGACATTAAAGCCTTA
AATAGTGAGATTTCTCAAATCGTGGAAAAACAAAGCGCTTTACGAAACCGCCTTGATCGTATTATCAAAGAGTTAGAAGC
TTAA

Upstream 100 bases:

>100_bases
CCAACGCCAAACAAAACCGCCAATCAAAAAAACACAAAAAAGGGGCTTATATCATGGAAAACAACCCAAACAATAACAAG
CGTTTTTAGAGCGCAACGAA

Downstream 100 bases:

>100_bases
GGGGGGCAAAATGCATAAAATAGAGCGCTTGCTCCAAACTCTAGCGCTTAAGGGGGTGGGGTTTAGGAAGTTGGGGGAGG
TGTGTGATTTTCAAAAAGGA

Product: type I restriction enzyme M protein

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MHNTIWKVANELRGSVDGWDFKQYVLGILFYRYISENMTHYINKKERKRDPDFDYAKLSDEEAKHARKHLIEEKDFFIPP
SALFCNALKNAPHNEDLNVTLQNIFNEIEKSSLGTPSEENVKGLFADLDVNSNKLGSSHKNRVEKLTKILEAIGGMQLGD
YLKSGIDVFGDAYEYLMAMYASNAGKSGGEFFTPQEVSELLAKITLHNQESVNKVYDPCCGSGSLLLQFSKVLGDKNVSK
GYFGQEINLTTYNLCRINMFLHDINYTKFHIALGDTLLDPKHEDDEPFDAIVSNPPYSTKWGGDNNPLLINDERFSPAGV
LAPKNAADLAFTMHMLSYLSNSGTAAIVEFPGVLYRGNAEAKIREYLVKENVIDCVIALPDNLFFGTNIATCILVLKKNK
KDDTTLFIDASKEFVKEGKKNKLKAHNREKILQTYIERKEVKHFCALANMEQIQENDYNLSVNRYVEQEDTKEAIDIKAL
NSEISQIVEKQSALRNRLDRIIKELEA

Sequences:

>Translated_507_residues
MHNTIWKVANELRGSVDGWDFKQYVLGILFYRYISENMTHYINKKERKRDPDFDYAKLSDEEAKHARKHLIEEKDFFIPP
SALFCNALKNAPHNEDLNVTLQNIFNEIEKSSLGTPSEENVKGLFADLDVNSNKLGSSHKNRVEKLTKILEAIGGMQLGD
YLKSGIDVFGDAYEYLMAMYASNAGKSGGEFFTPQEVSELLAKITLHNQESVNKVYDPCCGSGSLLLQFSKVLGDKNVSK
GYFGQEINLTTYNLCRINMFLHDINYTKFHIALGDTLLDPKHEDDEPFDAIVSNPPYSTKWGGDNNPLLINDERFSPAGV
LAPKNAADLAFTMHMLSYLSNSGTAAIVEFPGVLYRGNAEAKIREYLVKENVIDCVIALPDNLFFGTNIATCILVLKKNK
KDDTTLFIDASKEFVKEGKKNKLKAHNREKILQTYIERKEVKHFCALANMEQIQENDYNLSVNRYVEQEDTKEAIDIKAL
NSEISQIVEKQSALRNRLDRIIKELEA
>Mature_507_residues
MHNTIWKVANELRGSVDGWDFKQYVLGILFYRYISENMTHYINKKERKRDPDFDYAKLSDEEAKHARKHLIEEKDFFIPP
SALFCNALKNAPHNEDLNVTLQNIFNEIEKSSLGTPSEENVKGLFADLDVNSNKLGSSHKNRVEKLTKILEAIGGMQLGD
YLKSGIDVFGDAYEYLMAMYASNAGKSGGEFFTPQEVSELLAKITLHNQESVNKVYDPCCGSGSLLLQFSKVLGDKNVSK
GYFGQEINLTTYNLCRINMFLHDINYTKFHIALGDTLLDPKHEDDEPFDAIVSNPPYSTKWGGDNNPLLINDERFSPAGV
LAPKNAADLAFTMHMLSYLSNSGTAAIVEFPGVLYRGNAEAKIREYLVKENVIDCVIALPDNLFFGTNIATCILVLKKNK
KDDTTLFIDASKEFVKEGKKNKLKAHNREKILQTYIERKEVKHFCALANMEQIQENDYNLSVNRYVEQEDTKEAIDIKAL
NSEISQIVEKQSALRNRLDRIIKELEA

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=249, Percent_Identity=24.8995983935743, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 57472; Mature: 57472

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNTIWKVANELRGSVDGWDFKQYVLGILFYRYISENMTHYINKKERKRDPDFDYAKLSD
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHCCC
EEAKHARKHLIEEKDFFIPPSALFCNALKNAPHNEDLNVTLQNIFNEIEKSSLGTPSEEN
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCC
VKGLFADLDVNSNKLGSSHKNRVEKLTKILEAIGGMQLGDYLKSGIDVFGDAYEYLMAMY
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ASNAGKSGGEFFTPQEVSELLAKITLHNQESVNKVYDPCCGSGSLLLQFSKVLGDKNVSK
HHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCC
GYFGQEINLTTYNLCRINMFLHDINYTKFHIALGDTLLDPKHEDDEPFDAIVSNPPYSTK
CCCCCCCCCEEEEHEEEEEHHEECCCEEEEEEECCHHCCCCCCCCCCHHHHHCCCCCCCC
WGGDNNPLLINDERFSPAGVLAPKNAADLAFTMHMLSYLSNSGTAAIVEFPGVLYRGNAE
CCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCH
AKIREYLVKENVIDCVIALPDNLFFGTNIATCILVLKKNKKDDTTLFIDASKEFVKEGKK
HHHHHHHHHHHHHHEEEECCCCCEECCCHHHHHHHHCCCCCCCCEEEEECCHHHHHCCCH
NKLKAHNREKILQTYIERKEVKHFCALANMEQIQENDYNLSVNRYVEQEDTKEAIDIKAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCHHHHHHHHHHHHH
NSEISQIVEKQSALRNRLDRIIKELEA
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHNTIWKVANELRGSVDGWDFKQYVLGILFYRYISENMTHYINKKERKRDPDFDYAKLSD
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHCCC
EEAKHARKHLIEEKDFFIPPSALFCNALKNAPHNEDLNVTLQNIFNEIEKSSLGTPSEEN
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCC
VKGLFADLDVNSNKLGSSHKNRVEKLTKILEAIGGMQLGDYLKSGIDVFGDAYEYLMAMY
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ASNAGKSGGEFFTPQEVSELLAKITLHNQESVNKVYDPCCGSGSLLLQFSKVLGDKNVSK
HHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCC
GYFGQEINLTTYNLCRINMFLHDINYTKFHIALGDTLLDPKHEDDEPFDAIVSNPPYSTK
CCCCCCCCCEEEEHEEEEEHHEECCCEEEEEEECCHHCCCCCCCCCCHHHHHCCCCCCCC
WGGDNNPLLINDERFSPAGVLAPKNAADLAFTMHMLSYLSNSGTAAIVEFPGVLYRGNAE
CCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCH
AKIREYLVKENVIDCVIALPDNLFFGTNIATCILVLKKNKKDDTTLFIDASKEFVKEGKK
HHHHHHHHHHHHHHEEEECCCCCEECCCHHHHHHHHCCCCCCCCEEEEECCHHHHHCCCH
NKLKAHNREKILQTYIERKEVKHFCALANMEQIQENDYNLSVNRYVEQEDTKEAIDIKAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCHHHHHHHHHHHHH
NSEISQIVEKQSALRNRLDRIIKELEA
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]