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The map label for this gene is rsmD [H]

Identifier: 108563220

GI number: 108563220

Start: 832516

End: 833118

Strand: Reverse

Name: rsmD [H]

Synonym: HPAG1_0795

Alternate gene names: 108563220

Gene position: 833118-832516 (Counterclockwise)

Preceding gene: 108563221

Following gene: 108563219

Centisome position: 52.19

GC content: 36.65

Gene sequence:

>603_bases
ATGCCAAATCATCAGCCAGTAAAGAAATTTAAGATTATTGGGGGGGCTTGTAAGGGATTGGGCTTGAATTTGCCTAACGT
TTCTAGTACGCGCCCCACCAAAGCGATCGTAAGAGAGTCGTTTTTTAACACCTTGCAAGCAGAAATTAATGGAGCGCATT
TTATAGAAGTGTTTTCAGGCAGCGCTTCTATGGGTTTAGAGGCTTTGAGTAGGGGGGCAACAAGCGCGGTGTTTTTTGAG
CAAAACAAAAGCGCTTATAAGACGCTTTTAGAAAATATTTCCCTCTTTAAAAACCGCTTGAAAAAGGAAATTGAAATTCA
AACTTTTTTAGATGACGCTTTCAAGCTTTTACCCACGCTGTGTTTAAAAAATGGCGTTTTGAATATCCTTTATTTTGATC
CTCCTTTTGAAACAAGCGGGTTTTTAGGGATTTATGAAAAGTGTTTTCAAGCTTTAGAAAGGTTATTGAAACGCTCTAAT
CCAAAAAATCTTTTAGCGGTTTTTGAGCATGAAAGTGCGCATGAAATGCCTAAAAGTCTTGTAACTTTAGCTATAATCAA
ACAGAAAAAATTTGGAAAAACCACTTTAACTTATTTTCAATAG

Upstream 100 bases:

>100_bases
TTTATCGCGCTTTAAAGACGAGCGAAAAACCTTGCGAAGAAATCCAATTTTCTAAAGAGCAAAAGATTAAGCAATTGCTA
GAAGAATACACCCAAAAATT

Downstream 100 bases:

>100_bases
GAATAGGCATGGCAGAAGAACAAGAAAATACCGCGCAACAACCCCCCAAAAAAAGCAAAGCCCTTTTATTTGTCATCATT
GGAAGCGTGTTAGTGATGCT

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MPNHQPVKKFKIIGGACKGLGLNLPNVSSTRPTKAIVRESFFNTLQAEINGAHFIEVFSGSASMGLEALSRGATSAVFFE
QNKSAYKTLLENISLFKNRLKKEIEIQTFLDDAFKLLPTLCLKNGVLNILYFDPPFETSGFLGIYEKCFQALERLLKRSN
PKNLLAVFEHESAHEMPKSLVTLAIIKQKKFGKTTLTYFQ

Sequences:

>Translated_200_residues
MPNHQPVKKFKIIGGACKGLGLNLPNVSSTRPTKAIVRESFFNTLQAEINGAHFIEVFSGSASMGLEALSRGATSAVFFE
QNKSAYKTLLENISLFKNRLKKEIEIQTFLDDAFKLLPTLCLKNGVLNILYFDPPFETSGFLGIYEKCFQALERLLKRSN
PKNLLAVFEHESAHEMPKSLVTLAIIKQKKFGKTTLTYFQ
>Mature_199_residues
PNHQPVKKFKIIGGACKGLGLNLPNVSSTRPTKAIVRESFFNTLQAEINGAHFIEVFSGSASMGLEALSRGATSAVFFEQ
NKSAYKTLLENISLFKNRLKKEIEIQTFLDDAFKLLPTLCLKNGVLNILYFDPPFETSGFLGIYEKCFQALERLLKRSNP
KNLLAVFEHESAHEMPKSLVTLAIIKQKKFGKTTLTYFQ

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=199, Percent_Identity=28.1407035175879, Blast_Score=84, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22423; Mature: 22292

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNHQPVKKFKIIGGACKGLGLNLPNVSSTRPTKAIVRESFFNTLQAEINGAHFIEVFSG
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECC
SASMGLEALSRGATSAVFFEQNKSAYKTLLENISLFKNRLKKEIEIQTFLDDAFKLLPTL
CHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLKNGVLNILYFDPPFETSGFLGIYEKCFQALERLLKRSNPKNLLAVFEHESAHEMPKSL
HHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
VTLAIIKQKKFGKTTLTYFQ
HHHHHHHHHHCCCCEECCCC
>Mature Secondary Structure 
PNHQPVKKFKIIGGACKGLGLNLPNVSSTRPTKAIVRESFFNTLQAEINGAHFIEVFSG
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECC
SASMGLEALSRGATSAVFFEQNKSAYKTLLENISLFKNRLKKEIEIQTFLDDAFKLLPTL
CHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLKNGVLNILYFDPPFETSGFLGIYEKCFQALERLLKRSNPKNLLAVFEHESAHEMPKSL
HHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
VTLAIIKQKKFGKTTLTYFQ
HHHHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]