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The map label for this gene is gatB

Identifier: 108563068

GI number: 108563068

Start: 670440

End: 671867

Strand: Direct

Name: gatB

Synonym: HPAG1_0643

Alternate gene names: 108563068

Gene position: 670440-671867 (Clockwise)

Preceding gene: 108563067

Following gene: 108563069

Centisome position: 42.0

GC content: 42.02

Gene sequence:

>1428_bases
ATGATGCCATTTGAAGCTGTAATCGGGCTAGAAGTCCATGTCCAACTCAACACCAAAACCAAAATCTTTTGCTCTTGCTC
CACAAGCTTTGGAGAATCCCCCAATTCTAACACTTGCCCTGTGTGTTTGGGTTTACCGGGAGCTTTGCCGGTATTGAATA
AAGAAGTGGTTAAAAAAGCCATCCAATTAGGCACAGCCATTGAAGCCAATATCAACCAGTATTCCATTTTTGCGAGGAAA
AATTATTTTTACCCTGATTTGCCTAAGGCTTATCAAATTTCGCAGTTTGAAGTCCCCATTGTGAGCGATGGGAAATTAGA
GATTGACACTAAAGACGGCGCAAAAATCGTGCGTATTGAAAGGGTCCACATGGAAGAAGACGCCGGTAAAAATATCCATG
AGGGCAGCTATTCTTTGGTGGATTTGAACCGCGCTTGCACCCCTTTATTAGAAATTGTCAGTAAGCCGGACATGCGAAAT
AGTGAAGAAGCCATAGCGTATTTGAAAAAGCTCCATGCTATCGTGCGTTTTATAGGGATTTCTGATGCGAACATGCAAGA
GGGGAATTTCAGGTGCGATGCGAATGTGTCCATTAGACCCAAAGGCGATGAAAAGCTTTACACGAGGGTGGAGATTAAAA
ACTTAAATAGCTTTAGATTCATCGCTAAAGCGATTGAATACGAGATAGAGCGCCAAAGTGCGGCGTGGGAAAGCGGGCGT
TATCATGAAGAAGTGGTTCAAGAAACGCGCCTTTTTGACACCGCCAAAGGGATCACCCTTTCTATGCGCAATAAAGAAGA
ATCAGCGGATTACCGCTATTTTAAAGATCCGGATTTGTATCCTGTTTTTATTGATGAAAAACTTTTAAAAGAAGCTCAAA
AAATCAATGAATTGCCTGGCGCAAAAAAAATCCGCTATGTGAGAGATTTTAACCTTAAAGAAGACGATGCGAATTTATTG
GTGAGCGATCCTTTATTGGCGGAGTATTTTGAAAGCATGCTTAATCTTGGGGTTAAGGCTAAAACGAGCGTAACATGGCT
TTGCGTGGAATTATTAGGGCGCTTGAAAGCCGAAACCACTTTAGAAAATTGCGGAGTTAGCGCTCACATGTTAGGCGCTT
TAGCCAAACGCATTGATGAGGGCAAGATTTCAGGCAAGAGCGCTAAAGATGTGTTAGACAAGCTTTTAGAAGAGAAAGGG
GGCGATGTGGATGCGCTCATTGAACAAATGGGTCTGTCTCAAGTCAATGATACAGAAGCGATTGTTAAAGTGGTAGAAGA
GGTGCTTAAAAACAACGCTGATAAGGTGCTTGAATACAAAAGCGGTAAGGATAAGCTTTTTGGGTTTTTTGTAGGCCAAG
CGATGAAAAATTTAAAAGGCGCTAATCCTAGCGTGGTGAATGCTATTTTGAAAGAGAAATTGGATTGA

Upstream 100 bases:

>100_bases
TCCAAAAAATGAGTTTGAAAGAGATCAATGATTTCATTAAAGCCCACACCGAAATCAACGACTTGACTTTTGCCATTGTG
AGCAATAAAAAGAAGGACAA

Downstream 100 bases:

>100_bases
TGAGGAAAATTTTTTCTTATGTTTTGAAGGCTTTGCTGTTTTTTGGGATCGCTCATGCAGAGCCTGAATCTAAAGTAGAA
GCCTTAGAAGGGAAGAAACG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MMPFEAVIGLEVHVQLNTKTKIFCSCSTSFGESPNSNTCPVCLGLPGALPVLNKEVVKKAIQLGTAIEANINQYSIFARK
NYFYPDLPKAYQISQFEVPIVSDGKLEIDTKDGAKIVRIERVHMEEDAGKNIHEGSYSLVDLNRACTPLLEIVSKPDMRN
SEEAIAYLKKLHAIVRFIGISDANMQEGNFRCDANVSIRPKGDEKLYTRVEIKNLNSFRFIAKAIEYEIERQSAAWESGR
YHEEVVQETRLFDTAKGITLSMRNKEESADYRYFKDPDLYPVFIDEKLLKEAQKINELPGAKKIRYVRDFNLKEDDANLL
VSDPLLAEYFESMLNLGVKAKTSVTWLCVELLGRLKAETTLENCGVSAHMLGALAKRIDEGKISGKSAKDVLDKLLEEKG
GDVDALIEQMGLSQVNDTEAIVKVVEEVLKNNADKVLEYKSGKDKLFGFFVGQAMKNLKGANPSVVNAILKEKLD

Sequences:

>Translated_475_residues
MMPFEAVIGLEVHVQLNTKTKIFCSCSTSFGESPNSNTCPVCLGLPGALPVLNKEVVKKAIQLGTAIEANINQYSIFARK
NYFYPDLPKAYQISQFEVPIVSDGKLEIDTKDGAKIVRIERVHMEEDAGKNIHEGSYSLVDLNRACTPLLEIVSKPDMRN
SEEAIAYLKKLHAIVRFIGISDANMQEGNFRCDANVSIRPKGDEKLYTRVEIKNLNSFRFIAKAIEYEIERQSAAWESGR
YHEEVVQETRLFDTAKGITLSMRNKEESADYRYFKDPDLYPVFIDEKLLKEAQKINELPGAKKIRYVRDFNLKEDDANLL
VSDPLLAEYFESMLNLGVKAKTSVTWLCVELLGRLKAETTLENCGVSAHMLGALAKRIDEGKISGKSAKDVLDKLLEEKG
GDVDALIEQMGLSQVNDTEAIVKVVEEVLKNNADKVLEYKSGKDKLFGFFVGQAMKNLKGANPSVVNAILKEKLD
>Mature_475_residues
MMPFEAVIGLEVHVQLNTKTKIFCSCSTSFGESPNSNTCPVCLGLPGALPVLNKEVVKKAIQLGTAIEANINQYSIFARK
NYFYPDLPKAYQISQFEVPIVSDGKLEIDTKDGAKIVRIERVHMEEDAGKNIHEGSYSLVDLNRACTPLLEIVSKPDMRN
SEEAIAYLKKLHAIVRFIGISDANMQEGNFRCDANVSIRPKGDEKLYTRVEIKNLNSFRFIAKAIEYEIERQSAAWESGR
YHEEVVQETRLFDTAKGITLSMRNKEESADYRYFKDPDLYPVFIDEKLLKEAQKINELPGAKKIRYVRDFNLKEDDANLL
VSDPLLAEYFESMLNLGVKAKTSVTWLCVELLGRLKAETTLENCGVSAHMLGALAKRIDEGKISGKSAKDVLDKLLEEKG
GDVDALIEQMGLSQVNDTEAIVKVVEEVLKNNADKVLEYKSGKDKLFGFFVGQAMKNLKGANPSVVNAILKEKLD

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=498, Percent_Identity=34.5381526104418, Blast_Score=273, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI115534094, Length=354, Percent_Identity=37.2881355932203, Blast_Score=197, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6319391, Length=499, Percent_Identity=30.2605210420842, Blast_Score=198, Evalue=2e-51,
Organism=Drosophila melanogaster, GI45550808, Length=474, Percent_Identity=33.9662447257384, Blast_Score=251, Evalue=6e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_HELPH (Q1CTL2)

Other databases:

- EMBL:   CP000241
- RefSeq:   YP_627384.1
- ProteinModelPortal:   Q1CTL2
- SMR:   Q1CTL2
- STRING:   Q1CTL2
- GeneID:   4098769
- GenomeReviews:   CP000241_GR
- KEGG:   hpa:HPAG1_0643
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   DAKLENG
- ProtClustDB:   PRK05477
- BioCyc:   HPYL357544:HPAG1_0643-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53179; Mature: 53179

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPFEAVIGLEVHVQLNTKTKIFCSCSTSFGESPNSNTCPVCLGLPGALPVLNKEVVKKA
CCCCCEEEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
IQLGTAIEANINQYSIFARKNYFYPDLPKAYQISQFEVPIVSDGKLEIDTKDGAKIVRIE
HHHCCCEECCCCEEEEEEECCCCCCCCCCCEECCEEECEEECCCEEEEECCCCCEEEEEE
RVHMEEDAGKNIHEGSYSLVDLNRACTPLLEIVSKPDMRNSEEAIAYLKKLHAIVRFIGI
EEEEHHHCCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
SDANMQEGNFRCDANVSIRPKGDEKLYTRVEIKNLNSFRFIAKAIEYEIERQSAAWESGR
CCCCCCCCCEEECCCEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
YHEEVVQETRLFDTAKGITLSMRNKEESADYRYFKDPDLYPVFIDEKLLKEAQKINELPG
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCC
AKKIRYVRDFNLKEDDANLLVSDPLLAEYFESMLNLGVKAKTSVTWLCVELLGRLKAETT
CHHEEHEECCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LENCGVSAHMLGALAKRIDEGKISGKSAKDVLDKLLEEKGGDVDALIEQMGLSQVNDTEA
HHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHH
IVKVVEEVLKNNADKVLEYKSGKDKLFGFFVGQAMKNLKGANPSVVNAILKEKLD
HHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MMPFEAVIGLEVHVQLNTKTKIFCSCSTSFGESPNSNTCPVCLGLPGALPVLNKEVVKKA
CCCCCEEEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
IQLGTAIEANINQYSIFARKNYFYPDLPKAYQISQFEVPIVSDGKLEIDTKDGAKIVRIE
HHHCCCEECCCCEEEEEEECCCCCCCCCCCEECCEEECEEECCCEEEEECCCCCEEEEEE
RVHMEEDAGKNIHEGSYSLVDLNRACTPLLEIVSKPDMRNSEEAIAYLKKLHAIVRFIGI
EEEEHHHCCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
SDANMQEGNFRCDANVSIRPKGDEKLYTRVEIKNLNSFRFIAKAIEYEIERQSAAWESGR
CCCCCCCCCEEECCCEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
YHEEVVQETRLFDTAKGITLSMRNKEESADYRYFKDPDLYPVFIDEKLLKEAQKINELPG
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCC
AKKIRYVRDFNLKEDDANLLVSDPLLAEYFESMLNLGVKAKTSVTWLCVELLGRLKAETT
CHHEEHEECCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LENCGVSAHMLGALAKRIDEGKISGKSAKDVLDKLLEEKGGDVDALIEQMGLSQVNDTEA
HHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHH
IVKVVEEVLKNNADKVLEYKSGKDKLFGFFVGQAMKNLKGANPSVVNAILKEKLD
HHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA