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The map label for this gene is cca [H]

Identifier: 108563048

GI number: 108563048

Start: 650389

End: 651666

Strand: Direct

Name: cca [H]

Synonym: HPAG1_0623

Alternate gene names: 108563048

Gene position: 650389-651666 (Clockwise)

Preceding gene: 108563046

Following gene: 108563049

Centisome position: 40.74

GC content: 34.9

Gene sequence:

>1278_bases
GTGTTTGAGTTAGAAAAAGAATTAAAAGAATTGTTTGACATTTATGAAAAATCCCCTTATGAGCTTTACTTGGTAGGGGG
GTGCGTGCGCGATTGCTTGATGGGAATTACGCCTAAAGATTACGATTTAACCTCAAACGCTTTAGTCAATGAAAGCAAAG
AGATTCTTTTAAATAACCATTTTAGGGTGTTAGAAACCGGCATCAAACATGGCACGATCACGGCTCTTAAAAACCATCAA
AGCTATGAAATCACAACTTTTAGAATTGAAAAAGGGCATATCAAGCACCGAAAGCCTAAAGAATTGGTTTTTAGCTCTCG
TTTAACAGATGATTTAAAGCGGCGCGATTTTAGCATGAACGCGATCGCTTATAGCCCTACAAAAGGACTGATTGATCCTT
TTAAAGGGCAGAATGCGATTGAAAATCAAGTGATTGAATGCGTGGGGGGAGCGCGATTAAGGTTTTTTGAAGACGCTTTA
AGGATTTTAAGAGCGCTGCGATTTAGTGCGACTTTAGGCTTTAAGATAGTGCCAAGCACTAAAGAAGCGGTTTTTGCGTG
TAAGGATTTGTTAAAACACCTCTCCAAAGAGCGCTTACAAAGCGAGTTGAATAAGCTTCTTATGGGGAAAAACGCCTATG
AAGTGGCTAAAGAATATCAAGAAATTTTGGAGTTGGTTATTCAAGAAAAAATAGAAAATTTAGGGTTTTTAAAAAACGCG
CCTTTGAATTTGGAATTAAGATTGTTAGGGTTTTTTAAAGATCAAAAAAGTTTAGAAAATTTACGATACCCTAAAAAAAT
TTGTGTTTTATTTTTAAAAGCTAAAGAATGCCATAAAGCTTTTTTAAATACCCATAACAAAACAGAGTTAAAATTTTTAT
TGAAAAACTACGATTTAGAGCCTTTTAATTTGGCTTTAGATTTTTATGTGCTAGAAAACCCCAAACACGCTTTAAAAATC
AAAGGCTTGTTAAAAGAAATCTTTGATTCTAACGAGCCTTTTAAAAAAGAACACTTGGCCCTTAAGGGCGGTGCGCTCCA
AAGCTTGGGTTACCAACACCAAAAAATCGGCGAAATTTTAAACGCATGCTTAGATTTAGTCATTGAAAATCCTAAAAATA
ACGCTTTAGAGTGGCTGATAAAATGGGTTAAAGATAATTATTTACTTAATGATGCTATAAATCATTCGCCAATAGGCAGA
AAAATTAAAAACAGAGAAAACATGATAACGATGAATGCGATTCAATGGCCTAAGAAATGGATTTATAGGAGAGACTGA

Upstream 100 bases:

>100_bases
CCGCCACTAAAACTGACCACGCAAATTTCTTGTTCCATCAACATTCCTTAAAAATTCTAAAAAGATATAATAACACAAAG
AAATTTTAAGAGTAAAAAGC

Downstream 100 bases:

>100_bases
TAATTTTGTGTCCAATGAAGTCATTGTCAAAGGTTTGGATTTTAAAAAAGTGGTGCAGCATTTAATGGCGTATTCGTGCT
AATAAGTGAGGATTGAATGA

Product: poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MFELEKELKELFDIYEKSPYELYLVGGCVRDCLMGITPKDYDLTSNALVNESKEILLNNHFRVLETGIKHGTITALKNHQ
SYEITTFRIEKGHIKHRKPKELVFSSRLTDDLKRRDFSMNAIAYSPTKGLIDPFKGQNAIENQVIECVGGARLRFFEDAL
RILRALRFSATLGFKIVPSTKEAVFACKDLLKHLSKERLQSELNKLLMGKNAYEVAKEYQEILELVIQEKIENLGFLKNA
PLNLELRLLGFFKDQKSLENLRYPKKICVLFLKAKECHKAFLNTHNKTELKFLLKNYDLEPFNLALDFYVLENPKHALKI
KGLLKEIFDSNEPFKKEHLALKGGALQSLGYQHQKIGEILNACLDLVIENPKNNALEWLIKWVKDNYLLNDAINHSPIGR
KIKNRENMITMNAIQWPKKWIYRRD

Sequences:

>Translated_425_residues
MFELEKELKELFDIYEKSPYELYLVGGCVRDCLMGITPKDYDLTSNALVNESKEILLNNHFRVLETGIKHGTITALKNHQ
SYEITTFRIEKGHIKHRKPKELVFSSRLTDDLKRRDFSMNAIAYSPTKGLIDPFKGQNAIENQVIECVGGARLRFFEDAL
RILRALRFSATLGFKIVPSTKEAVFACKDLLKHLSKERLQSELNKLLMGKNAYEVAKEYQEILELVIQEKIENLGFLKNA
PLNLELRLLGFFKDQKSLENLRYPKKICVLFLKAKECHKAFLNTHNKTELKFLLKNYDLEPFNLALDFYVLENPKHALKI
KGLLKEIFDSNEPFKKEHLALKGGALQSLGYQHQKIGEILNACLDLVIENPKNNALEWLIKWVKDNYLLNDAINHSPIGR
KIKNRENMITMNAIQWPKKWIYRRD
>Mature_425_residues
MFELEKELKELFDIYEKSPYELYLVGGCVRDCLMGITPKDYDLTSNALVNESKEILLNNHFRVLETGIKHGTITALKNHQ
SYEITTFRIEKGHIKHRKPKELVFSSRLTDDLKRRDFSMNAIAYSPTKGLIDPFKGQNAIENQVIECVGGARLRFFEDAL
RILRALRFSATLGFKIVPSTKEAVFACKDLLKHLSKERLQSELNKLLMGKNAYEVAKEYQEILELVIQEKIENLGFLKNA
PLNLELRLLGFFKDQKSLENLRYPKKICVLFLKAKECHKAFLNTHNKTELKFLLKNYDLEPFNLALDFYVLENPKHALKI
KGLLKEIFDSNEPFKKEHLALKGGALQSLGYQHQKIGEILNACLDLVIENPKNNALEWLIKWVKDNYLLNDAINHSPIGR
KIKNRENMITMNAIQWPKKWIYRRD

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=209, Percent_Identity=34.9282296650718, Blast_Score=113, Evalue=4e-25,
Organism=Escherichia coli, GI87081691, Length=225, Percent_Identity=32, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789436, Length=197, Percent_Identity=31.4720812182741, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71995920, Length=223, Percent_Identity=36.322869955157, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281360112, Length=406, Percent_Identity=27.0935960591133, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21357337, Length=406, Percent_Identity=27.0935960591133, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 49396; Mature: 49396

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFELEKELKELFDIYEKSPYELYLVGGCVRDCLMGITPKDYDLTSNALVNESKEILLNNH
CCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHCCC
FRVLETGIKHGTITALKNHQSYEITTFRIEKGHIKHRKPKELVFSSRLTDDLKRRDFSMN
HHHHHHCCCCCEEEEEECCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
AIAYSPTKGLIDPFKGQNAIENQVIECVGGARLRFFEDALRILRALRFSATLGFKIVPST
EEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCC
KEAVFACKDLLKHLSKERLQSELNKLLMGKNAYEVAKEYQEILELVIQEKIENLGFLKNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PLNLELRLLGFFKDQKSLENLRYPKKICVLFLKAKECHKAFLNTHNKTELKFLLKNYDLE
CCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
PFNLALDFYVLENPKHALKIKGLLKEIFDSNEPFKKEHLALKGGALQSLGYQHQKIGEIL
CEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHCCHHHHHHHHH
NACLDLVIENPKNNALEWLIKWVKDNYLLNDAINHSPIGRKIKNRENMITMNAIQWPKKW
HHHHHHHHCCCCCHHHHHHHHHHHCCEEECCCCCCCCCCHHHHCCCCEEEEHHHHCCHHH
IYRRD
CCCCC
>Mature Secondary Structure
MFELEKELKELFDIYEKSPYELYLVGGCVRDCLMGITPKDYDLTSNALVNESKEILLNNH
CCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHCCC
FRVLETGIKHGTITALKNHQSYEITTFRIEKGHIKHRKPKELVFSSRLTDDLKRRDFSMN
HHHHHHCCCCCEEEEEECCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
AIAYSPTKGLIDPFKGQNAIENQVIECVGGARLRFFEDALRILRALRFSATLGFKIVPST
EEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCC
KEAVFACKDLLKHLSKERLQSELNKLLMGKNAYEVAKEYQEILELVIQEKIENLGFLKNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PLNLELRLLGFFKDQKSLENLRYPKKICVLFLKAKECHKAFLNTHNKTELKFLLKNYDLE
CCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
PFNLALDFYVLENPKHALKIKGLLKEIFDSNEPFKKEHLALKGGALQSLGYQHQKIGEIL
CEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHCCHHHHHHHHH
NACLDLVIENPKNNALEWLIKWVKDNYLLNDAINHSPIGRKIKNRENMITMNAIQWPKKW
HHHHHHHHCCCCCHHHHHHHHHHHCCEEECCCCCCCCCCHHHHCCCCEEEEHHHHCCHHH
IYRRD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA