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The map label for this gene is hydB [H]

Identifier: 108563040

GI number: 108563040

Start: 642967

End: 644703

Strand: Direct

Name: hydB [H]

Synonym: HPAG1_0615

Alternate gene names: 108563040

Gene position: 642967-644703 (Clockwise)

Preceding gene: 108563039

Following gene: 108563041

Centisome position: 40.28

GC content: 42.77

Gene sequence:

>1737_bases
ATGTCAAAAAAAATCGTAGTCGATCCTATCACTAGGATTGAGGGGCATTTAAGGATTGAAGTGATCGTAGATGATGATAA
TGTGATCACCGATGCGTTTTCTTCTTCTACGCTTTTTAGGGGGCTAGAGACCATTATTAAAGGCAGAGACCCACGAGATG
CAGGTTTTATCGCTCAAAGGATTTGCGGGGTATGCACTTATTCGCATTATAAGGCCGGTATCACTGCGGTAGAAAACGCT
CTAGGCATCACCCCTCCATTAAACGCGCAATTGGTGCGATCTTTGATGAACATGGCGTTGCTTTTTCATGACCATGTGGT
GCATTTTTATACTTTGCATGGGCTTGATTGGTGCGATATTATGAGTGCTTTAAAAGCCGATCCCATTCAAGCGGCAAAAC
TTTCTTTCAAATACAGCCCTTATCCCATTAATACCGGTGCCGGTGAATTAAAAGCGGTTCAAAAACGCTTGAATGATTTC
GCTAAAGGCGGATCTTTGGGGCCTTTTAGTAATGGCTATTATGGGCATAAAACCTATCGTTTAAGCCCAGAGCAAAATTT
AATCGTCTTAAGCCACTACCTCAAGCTTTTAGAAATCCAAAGGGAAGCGGCGAAAATGACCGCTATTTTTGGGGCCAAAC
AGCCTCACCCACAAAGCCTAACGGTGGGGGGTGTTACGAGTGTTATGGATATATTGGATCCGACGAGATTGGCTGAATGG
AAGAGCAAGTTTGAAGTGGTGGCCAATTTCATCAACCATGCTTACTACCCTGATTTGGTAATGGCGGGCGAAATGTTCGC
TAACGAACAATCGGTTATTAAAGGCTGTGGCTTAAGGAATTTTATCGCTTATGAAGAAGTGTTGCTCGGGAGGGATAAAT
ACCTTTTGAGTAGTGGGGTGGTGCTTGATGGGGATATTTCTAAACTGCACCCCATTGATGAGAGTTTGATTAAAGAAGAA
GTTACGCATTCTTGGTATCAATACGAAGACACTAAAGAGGTGCAACTCCACCCTTATGACGGGCAAACGAACCCACATTA
TACCGGTTTAAAAGACGGCGAGAGCGTGGGGATTGAAAATAAAATCATCCCTGCTAAAGTGCTTGACACTAAAAATAAAT
ATTCTTGGATCAAATCGCCCAGATACGATAGTAAGCCTATGGAAGTAGGCCCTTTAAGTTCCGTAGTGGTAGGTTTAGCG
GCGAAAAACCCTTATGTTACTGAAGTGGCTACGAAGTTTTTAAAAGACACGAAACTGCCTTTAGAGGCGTTGTTTTCAAC
GCTTGGGCGCACCGCTGCAAGGTGTATTGAAGCTAAAACGATCGCTGATAATGGCCTTTTAGCGTTTGATGCACTAGTGG
AAAATCTAAAAAGCGATCAAAGCACTTGCGCTCCTTATCATATTGATAAAAATCAAGAATATAAAGGGCGCTACATTGGT
CAAGTGCCAAGGGGCATGCTAAGCCATTGGGTGCGTATTAAAAATGGCGTGGTGGAAAATTATCAAGCGGTAGTGCCTTC
TACTTGGAATGCAGGGCCTAGAGATTCTCAAAATCAAAGGGGGGCTTATGAAATGAGCTTGATTGGCACTAAAATCGCTG
ATTTAACCCAGCCTTTAGAAATCATTAGGACTATCCATTCTTTTGACCCATGCATCGCATGCTCAGTGCATGTGATGGAT
TTTAAAGGGCAGTCTTTAAACGAGTTTAAAGTAGAGCCTAATTTCGCTAAATTCTAA

Upstream 100 bases:

>100_bases
GGATAAAGTAGGCACGACTCTACTCAGCGCAACCGCTATTGGCATTGTCGCGCATGCGCTCCTTTCTAAAGCGATCAAAA
ACAAAGAGTAAGGGATTAAC

Downstream 100 bases:

>100_bases
AAAAGGGTTACGCATGGATAAAATGAATAAGGTCGTTTTACACAAAGAATATTCCGGTTTTGTGCGCTTTTTCCATTGGG
TTAGGGCTTTGAGTATTTTT

Product: quinone-reactive Ni/Fe hydrogenase HydB

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MSKKIVVDPITRIEGHLRIEVIVDDDNVITDAFSSSTLFRGLETIIKGRDPRDAGFIAQRICGVCTYSHYKAGITAVENA
LGITPPLNAQLVRSLMNMALLFHDHVVHFYTLHGLDWCDIMSALKADPIQAAKLSFKYSPYPINTGAGELKAVQKRLNDF
AKGGSLGPFSNGYYGHKTYRLSPEQNLIVLSHYLKLLEIQREAAKMTAIFGAKQPHPQSLTVGGVTSVMDILDPTRLAEW
KSKFEVVANFINHAYYPDLVMAGEMFANEQSVIKGCGLRNFIAYEEVLLGRDKYLLSSGVVLDGDISKLHPIDESLIKEE
VTHSWYQYEDTKEVQLHPYDGQTNPHYTGLKDGESVGIENKIIPAKVLDTKNKYSWIKSPRYDSKPMEVGPLSSVVVGLA
AKNPYVTEVATKFLKDTKLPLEALFSTLGRTAARCIEAKTIADNGLLAFDALVENLKSDQSTCAPYHIDKNQEYKGRYIG
QVPRGMLSHWVRIKNGVVENYQAVVPSTWNAGPRDSQNQRGAYEMSLIGTKIADLTQPLEIIRTIHSFDPCIACSVHVMD
FKGQSLNEFKVEPNFAKF

Sequences:

>Translated_578_residues
MSKKIVVDPITRIEGHLRIEVIVDDDNVITDAFSSSTLFRGLETIIKGRDPRDAGFIAQRICGVCTYSHYKAGITAVENA
LGITPPLNAQLVRSLMNMALLFHDHVVHFYTLHGLDWCDIMSALKADPIQAAKLSFKYSPYPINTGAGELKAVQKRLNDF
AKGGSLGPFSNGYYGHKTYRLSPEQNLIVLSHYLKLLEIQREAAKMTAIFGAKQPHPQSLTVGGVTSVMDILDPTRLAEW
KSKFEVVANFINHAYYPDLVMAGEMFANEQSVIKGCGLRNFIAYEEVLLGRDKYLLSSGVVLDGDISKLHPIDESLIKEE
VTHSWYQYEDTKEVQLHPYDGQTNPHYTGLKDGESVGIENKIIPAKVLDTKNKYSWIKSPRYDSKPMEVGPLSSVVVGLA
AKNPYVTEVATKFLKDTKLPLEALFSTLGRTAARCIEAKTIADNGLLAFDALVENLKSDQSTCAPYHIDKNQEYKGRYIG
QVPRGMLSHWVRIKNGVVENYQAVVPSTWNAGPRDSQNQRGAYEMSLIGTKIADLTQPLEIIRTIHSFDPCIACSVHVMD
FKGQSLNEFKVEPNFAKF
>Mature_577_residues
SKKIVVDPITRIEGHLRIEVIVDDDNVITDAFSSSTLFRGLETIIKGRDPRDAGFIAQRICGVCTYSHYKAGITAVENAL
GITPPLNAQLVRSLMNMALLFHDHVVHFYTLHGLDWCDIMSALKADPIQAAKLSFKYSPYPINTGAGELKAVQKRLNDFA
KGGSLGPFSNGYYGHKTYRLSPEQNLIVLSHYLKLLEIQREAAKMTAIFGAKQPHPQSLTVGGVTSVMDILDPTRLAEWK
SKFEVVANFINHAYYPDLVMAGEMFANEQSVIKGCGLRNFIAYEEVLLGRDKYLLSSGVVLDGDISKLHPIDESLIKEEV
THSWYQYEDTKEVQLHPYDGQTNPHYTGLKDGESVGIENKIIPAKVLDTKNKYSWIKSPRYDSKPMEVGPLSSVVVGLAA
KNPYVTEVATKFLKDTKLPLEALFSTLGRTAARCIEAKTIADNGLLAFDALVENLKSDQSTCAPYHIDKNQEYKGRYIGQ
VPRGMLSHWVRIKNGVVENYQAVVPSTWNAGPRDSQNQRGAYEMSLIGTKIADLTQPLEIIRTIHSFDPCIACSVHVMDF
KGQSLNEFKVEPNFAKF

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=593, Percent_Identity=41.3153456998314, Blast_Score=450, Evalue=1e-127,
Organism=Escherichia coli, GI1789368, Length=595, Percent_Identity=41.0084033613445, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1789076, Length=96, Percent_Identity=32.2916666666667, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 64394; Mature: 64263

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKIVVDPITRIEGHLRIEVIVDDDNVITDAFSSSTLFRGLETIIKGRDPRDAGFIAQR
CCCCEEECCHHHCCCEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ICGVCTYSHYKAGITAVENALGITPPLNAQLVRSLMNMALLFHDHVVHFYTLHGLDWCDI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHH
MSALKADPIQAAKLSFKYSPYPINTGAGELKAVQKRLNDFAKGGSLGPFSNGYYGHKTYR
HHHHCCCCCCHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
LSPEQNLIVLSHYLKLLEIQREAAKMTAIFGAKQPHPQSLTVGGVTSVMDILDPTRLAEW
ECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHCHHHHHHH
KSKFEVVANFINHAYYPDLVMAGEMFANEQSVIKGCGLRNFIAYEEVLLGRDKYLLSSGV
HHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCC
VLDGDISKLHPIDESLIKEEVTHSWYQYEDTKEVQLHPYDGQTNPHYTGLKDGESVGIEN
EEECCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
KIIPAKVLDTKNKYSWIKSPRYDSKPMEVGPLSSVVVGLAAKNPYVTEVATKFLKDTKLP
CCCCHHHHCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
LEALFSTLGRTAARCIEAKTIADNGLLAFDALVENLKSDQSTCAPYHIDKNQEYKGRYIG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHH
QVPRGMLSHWVRIKNGVVENYQAVVPSTWNAGPRDSQNQRGAYEMSLIGTKIADLTQPLE
HCHHHHHHHHHHHHCCHHHCCCEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
IIRTIHSFDPCIACSVHVMDFKGQSLNEFKVEPNFAKF
HHHHHHHCCCHHEEEEEEEECCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
SKKIVVDPITRIEGHLRIEVIVDDDNVITDAFSSSTLFRGLETIIKGRDPRDAGFIAQR
CCCEEECCHHHCCCEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ICGVCTYSHYKAGITAVENALGITPPLNAQLVRSLMNMALLFHDHVVHFYTLHGLDWCDI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHH
MSALKADPIQAAKLSFKYSPYPINTGAGELKAVQKRLNDFAKGGSLGPFSNGYYGHKTYR
HHHHCCCCCCHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
LSPEQNLIVLSHYLKLLEIQREAAKMTAIFGAKQPHPQSLTVGGVTSVMDILDPTRLAEW
ECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHCHHHHHHH
KSKFEVVANFINHAYYPDLVMAGEMFANEQSVIKGCGLRNFIAYEEVLLGRDKYLLSSGV
HHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCC
VLDGDISKLHPIDESLIKEEVTHSWYQYEDTKEVQLHPYDGQTNPHYTGLKDGESVGIEN
EEECCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
KIIPAKVLDTKNKYSWIKSPRYDSKPMEVGPLSSVVVGLAAKNPYVTEVATKFLKDTKLP
CCCCHHHHCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
LEALFSTLGRTAARCIEAKTIADNGLLAFDALVENLKSDQSTCAPYHIDKNQEYKGRYIG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHH
QVPRGMLSHWVRIKNGVVENYQAVVPSTWNAGPRDSQNQRGAYEMSLIGTKIADLTQPLE
HCHHHHHHHHHHHHCCHHHCCCEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
IIRTIHSFDPCIACSVHVMDFKGQSLNEFKVEPNFAKF
HHHHHHHCCCHHEEEEEEEECCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]