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The map label for this gene is hydA [H]

Identifier: 108563039

GI number: 108563039

Start: 641803

End: 642957

Strand: Direct

Name: hydA [H]

Synonym: HPAG1_0614

Alternate gene names: 108563039

Gene position: 641803-642957 (Clockwise)

Preceding gene: 108563037

Following gene: 108563040

Centisome position: 40.2

GC content: 46.58

Gene sequence:

>1155_bases
ATGTTCTACGATGAAAAAAAGACCTATCAAAAGATTGAAGAACGCCTTGATATAGTCAGTTCGTTTAACGCTCACAACGA
GCATAAAAACTTGCAAGATGAGTTTAAAGGGGCTGGCATTTCCAGGCGCGATTTATTGAAGTGGGCGGGCATGATGAGCG
CAGCGTTAGCCTTGCCGGCTAGTTTTGCTCCCTTGACTTTGAAGGCGGTAGAAGTGGCTAACAGATTGCCCGTGATTTGG
TTGCACATGGCAGAATGCACCGGTTGTAGCGAAAGCTTGTTAAGGAGCGCAGACCCCACTATTGATAGCATTATCTTTGA
TTACATCAACCTAGAATACCATGAAACTATCATGGTAGCGAGCGGTTTTCAGGCTGAAAAAAGCTTGCATGACGCCATAG
AAAAGCATAAAAACAATTACATTTTAATGGTAGAAGGCGGTATCCCCCAAGGCACCGAATACTTTCTCACTCAAGGCCCA
AACGCTGAAACGGGGGCTGAAGAGTGCAGGAAGGCCGCTCAATACGCAGCGGCTATTTTTGCCATAGGCACATGCTCAAG
TTTTGGGGGCGTGCAAGCGGCTTACCCTAACCCCTCTAACGCGCAACCCTTGCATAAAATCATTGACAAACCCGTGATCA
ATGTTCCCGGTTGCCCGCCTAGTGAAAAAAATATCGTGGGCAATGTGCTTTATTACTTGATGTTTGGGGCTCTCCCTAAA
TTAGATGCGTATAACCGCCCCTCTTGGGCTTATGGGAACAGGATCCATGATTTGTGCGAAAGGCGAGGGCATTTTGATGC
GGGCGAATTTGTGGAGCATTTTGGCGATGAAAACGCTAAAAGGGGCTTTTGTTTGTATAAAATGGGCTGTAAAGGGCCTT
ACACTTTCAACAATTGCTCCAAACTCCGCTTCAATTCACACACCTCTTGGCCCATAGGCGCAGGGCATGGGTGCATAGGG
TGTTCTGAGCCTAATTTTTGGGATACGATGAGTCCTTTTGAAGAACCTTTAGCGAATCGTTCCATTAAAACCGCCTTTGA
TGGATTAGGGGCTGATAAAGTAGCGGATAAAGTAGGCACGACTCTACTCAGCGCAACCGCTATTGGCATTGTCGCGCATG
CGCTCCTTTCTAAAGCGATCAAAAACAAAGAGTAA

Upstream 100 bases:

>100_bases
CGATTATACCTCAAAAGGGTATTTTTAAAAAGCGATTAAATTGCAACTGTATTATTTTTATTATGTTAAGATAATGAAAA
TTTCCAATTAAGGAGTGGTC

Downstream 100 bases:

>100_bases
GGGATTAACATGTCAAAAAAAATCGTAGTCGATCCTATCACTAGGATTGAGGGGCATTTAAGGATTGAAGTGATCGTAGA
TGATGATAATGTGATCACCG

Product: quinone-reactive Ni/Fe hydrogenase HydA

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MFYDEKKTYQKIEERLDIVSSFNAHNEHKNLQDEFKGAGISRRDLLKWAGMMSAALALPASFAPLTLKAVEVANRLPVIW
LHMAECTGCSESLLRSADPTIDSIIFDYINLEYHETIMVASGFQAEKSLHDAIEKHKNNYILMVEGGIPQGTEYFLTQGP
NAETGAEECRKAAQYAAAIFAIGTCSSFGGVQAAYPNPSNAQPLHKIIDKPVINVPGCPPSEKNIVGNVLYYLMFGALPK
LDAYNRPSWAYGNRIHDLCERRGHFDAGEFVEHFGDENAKRGFCLYKMGCKGPYTFNNCSKLRFNSHTSWPIGAGHGCIG
CSEPNFWDTMSPFEEPLANRSIKTAFDGLGADKVADKVGTTLLSATAIGIVAHALLSKAIKNKE

Sequences:

>Translated_384_residues
MFYDEKKTYQKIEERLDIVSSFNAHNEHKNLQDEFKGAGISRRDLLKWAGMMSAALALPASFAPLTLKAVEVANRLPVIW
LHMAECTGCSESLLRSADPTIDSIIFDYINLEYHETIMVASGFQAEKSLHDAIEKHKNNYILMVEGGIPQGTEYFLTQGP
NAETGAEECRKAAQYAAAIFAIGTCSSFGGVQAAYPNPSNAQPLHKIIDKPVINVPGCPPSEKNIVGNVLYYLMFGALPK
LDAYNRPSWAYGNRIHDLCERRGHFDAGEFVEHFGDENAKRGFCLYKMGCKGPYTFNNCSKLRFNSHTSWPIGAGHGCIG
CSEPNFWDTMSPFEEPLANRSIKTAFDGLGADKVADKVGTTLLSATAIGIVAHALLSKAIKNKE
>Mature_384_residues
MFYDEKKTYQKIEERLDIVSSFNAHNEHKNLQDEFKGAGISRRDLLKWAGMMSAALALPASFAPLTLKAVEVANRLPVIW
LHMAECTGCSESLLRSADPTIDSIIFDYINLEYHETIMVASGFQAEKSLHDAIEKHKNNYILMVEGGIPQGTEYFLTQGP
NAETGAEECRKAAQYAAAIFAIGTCSSFGGVQAAYPNPSNAQPLHKIIDKPVINVPGCPPSEKNIVGNVLYYLMFGALPK
LDAYNRPSWAYGNRIHDLCERRGHFDAGEFVEHFGDENAKRGFCLYKMGCKGPYTFNNCSKLRFNSHTSWPIGAGHGCIG
CSEPNFWDTMSPFEEPLANRSIKTAFDGLGADKVADKVGTTLLSATAIGIVAHALLSKAIKNKE

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=339, Percent_Identity=43.3628318584071, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI1789371, Length=289, Percent_Identity=46.3667820069204, Blast_Score=255, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 42255; Mature: 42255

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYDEKKTYQKIEERLDIVSSFNAHNEHKNLQDEFKGAGISRRDLLKWAGMMSAALALPA
CCCCHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
SFAPLTLKAVEVANRLPVIWLHMAECTGCSESLLRSADPTIDSIIFDYINLEYHETIMVA
CCCCHHHHHHHHHCCCCEEEEEHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCHHHEEEEE
SGFQAEKSLHDAIEKHKNNYILMVEGGIPQGTEYFLTQGPNAETGAEECRKAAQYAAAIF
CCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
AIGTCSSFGGVQAAYPNPSNAQPLHKIIDKPVINVPGCPPSEKNIVGNVLYYLMFGALPK
HHHHCCCCCCEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
LDAYNRPSWAYGNRIHDLCERRGHFDAGEFVEHFGDENAKRGFCLYKMGCKGPYTFNNCS
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCC
KLRFNSHTSWPIGAGHGCIGCSEPNFWDTMSPFEEPLANRSIKTAFDGLGADKVADKVGT
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHCCCHHHHHHHHHH
TLLSATAIGIVAHALLSKAIKNKE
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFYDEKKTYQKIEERLDIVSSFNAHNEHKNLQDEFKGAGISRRDLLKWAGMMSAALALPA
CCCCHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
SFAPLTLKAVEVANRLPVIWLHMAECTGCSESLLRSADPTIDSIIFDYINLEYHETIMVA
CCCCHHHHHHHHHCCCCEEEEEHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCHHHEEEEE
SGFQAEKSLHDAIEKHKNNYILMVEGGIPQGTEYFLTQGPNAETGAEECRKAAQYAAAIF
CCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
AIGTCSSFGGVQAAYPNPSNAQPLHKIIDKPVINVPGCPPSEKNIVGNVLYYLMFGALPK
HHHHCCCCCCEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
LDAYNRPSWAYGNRIHDLCERRGHFDAGEFVEHFGDENAKRGFCLYKMGCKGPYTFNNCS
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCC
KLRFNSHTSWPIGAGHGCIGCSEPNFWDTMSPFEEPLANRSIKTAFDGLGADKVADKVGT
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHCCCHHHHHHHHHH
TLLSATAIGIVAHALLSKAIKNKE
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]