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The map label for this gene is pldA [C]

Identifier: 108562900

GI number: 108562900

Start: 491138

End: 492205

Strand: Direct

Name: pldA [C]

Synonym: HPAG1_0475

Alternate gene names: 108562900

Gene position: 491138-492205 (Clockwise)

Preceding gene: 108562899

Following gene: 108562901

Centisome position: 30.77

GC content: 40.17

Gene sequence:

>1068_bases
ATGAAAAGCATTTTGCTCTTTATGATTTTTGTAGTTTGTCAGTTAGAAGGCAAAAAATTTTCACAAGATAATTTTAAGGT
GGATTATAACTACTATTTGCGCAAACAGGATTTGCACATCATTAAAACGCAAAACGATTTGTCCAATGCCTGGTATCTCC
CTCCACAAAAAGCCCCTAAAGAACATTCTTGGGTGGATTTTGCTAAAAAATATTTAAACATGATGGATTATCTAGGCACT
TATTTTTTGCCTTTTTATCATAGTTTCACCCCCATTTTTCAATGGTACCACCCCAATATCAACCCCTATCAACGCAATGA
GTTTAAGTTCCAAATTAGTTTTAGAGTGCCTGTATTTAGGCATATTCTTTGGACTAAAGGCACGCTTTATTTGGCTTATA
CCCAAACTAACTGGTTTCAAATTTATAATGACCCCCAATCCGCTCCCATGCGAATGATCAATTTCATGCCTGAACTCATC
TATGTTTATCCTATTAATTTTAAACCTTTTGGGGGTAAGATAGGGAATTTTTCTGAAATTTGGATAGGTTGGCAGCACAT
TTCTAATGGCGTGGGAGGCGCGCAATGTTACCAACCTTTTAATAAAGAAGGTAATCCTGAAAACCAATTCCCAGGAGGGC
CTGTGATCGTTAAAGATTATAACGGGCAAAAAGATGTGCGTTGGGGGGGGTGTCGTTCGGTGAGCGCGGGGCAACGCCCT
GTGTTTCGTTTGGTGTGGGAAAAGGGAGGTTTGAAAATCATGGTCGCTTATTGGCCCTATGTCCCTTATGATCAATCCAA
CCCTAATTTGATTGATTACATGGGGTATGGGAACGCTAAAATTGATTACAGGAGAGGGCGCCACCATTTTGAATTGCAAC
TTTATGATATTTTCACGCAATACTGGCGTTATGATCGCTGGCATGGAGCTTTCCGCTTAGGCTATACCTACCGCATTAAC
CCTTTTGTGGGGATTTATGCGCAGTGGTTTAACGGCTATGGCGATGGCTTGTATGAATACGATGTTTTTTCCAATCGTAT
AGGGGTAGGAATACGCTTAAACCCTTAA

Upstream 100 bases:

>100_bases
CAAACGCTTTTAAAGCGTGGCATTTTAGCGTTCGTTTTATCGCGCCTGTAGTGATTTTAGCGATTTTCATCTTGCAATTT
AAGTGAAATAAAGATACTTG

Downstream 100 bases:

>100_bases
AAAAGCGTTCTTTTATGCTATAATTAAGACCAAAAATTCAAGGGGATTATTCAGCTATGAAAATCAGTGTTAGTAAAAAC
GATTTAGAGAATGCTTTGCG

Product: phospholipase A1

Products: 2-acylglycerophosphocholine; carboxylate

Alternate protein names: Outer Membrane Phospholipase A

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKSILLFMIFVVCQLEGKKFSQDNFKVDYNYYLRKQDLHIIKTQNDLSNAWYLPPQKAPKEHSWVDFAKKYLNMMDYLGT
YFLPFYHSFTPIFQWYHPNINPYQRNEFKFQISFRVPVFRHILWTKGTLYLAYTQTNWFQIYNDPQSAPMRMINFMPELI
YVYPINFKPFGGKIGNFSEIWIGWQHISNGVGGAQCYQPFNKEGNPENQFPGGPVIVKDYNGQKDVRWGGCRSVSAGQRP
VFRLVWEKGGLKIMVAYWPYVPYDQSNPNLIDYMGYGNAKIDYRRGRHHFELQLYDIFTQYWRYDRWHGAFRLGYTYRIN
PFVGIYAQWFNGYGDGLYEYDVFSNRIGVGIRLNP

Sequences:

>Translated_355_residues
MKSILLFMIFVVCQLEGKKFSQDNFKVDYNYYLRKQDLHIIKTQNDLSNAWYLPPQKAPKEHSWVDFAKKYLNMMDYLGT
YFLPFYHSFTPIFQWYHPNINPYQRNEFKFQISFRVPVFRHILWTKGTLYLAYTQTNWFQIYNDPQSAPMRMINFMPELI
YVYPINFKPFGGKIGNFSEIWIGWQHISNGVGGAQCYQPFNKEGNPENQFPGGPVIVKDYNGQKDVRWGGCRSVSAGQRP
VFRLVWEKGGLKIMVAYWPYVPYDQSNPNLIDYMGYGNAKIDYRRGRHHFELQLYDIFTQYWRYDRWHGAFRLGYTYRIN
PFVGIYAQWFNGYGDGLYEYDVFSNRIGVGIRLNP
>Mature_355_residues
MKSILLFMIFVVCQLEGKKFSQDNFKVDYNYYLRKQDLHIIKTQNDLSNAWYLPPQKAPKEHSWVDFAKKYLNMMDYLGT
YFLPFYHSFTPIFQWYHPNINPYQRNEFKFQISFRVPVFRHILWTKGTLYLAYTQTNWFQIYNDPQSAPMRMINFMPELI
YVYPINFKPFGGKIGNFSEIWIGWQHISNGVGGAQCYQPFNKEGNPENQFPGGPVIVKDYNGQKDVRWGGCRSVSAGQRP
VFRLVWEKGGLKIMVAYWPYVPYDQSNPNLIDYMGYGNAKIDYRRGRHHFELQLYDIFTQYWRYDRWHGAFRLGYTYRIN
PFVGIYAQWFNGYGDGLYEYDVFSNRIGVGIRLNP

Specific function: Hydrolysis Of Phosphatidylcholine With Phospholipase A2 (Ec 3.1.1.4) And Phospholipase A1 (Ec 3.1.1.32) Activities. Required For Efficient Secretion Of Bacteriocins; Seems To Be Dormant In Normal Growing Cells. [C]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Outer Membrane; One Of The Very Few Enzymes Located There [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI2367300, Length=251, Percent_Identity=27.4900398406374, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.1.1.32

Molecular weight: Translated: 42381; Mature: 42381

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSILLFMIFVVCQLEGKKFSQDNFKVDYNYYLRKQDLHIIKTQNDLSNAWYLPPQKAPK
CHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCEEEEEECCCCCCEEECCCCCCCC
EHSWVDFAKKYLNMMDYLGTYFLPFYHSFTPIFQWYHPNINPYQRNEFKFQISFRVPVFR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCEEEEEEEEECHHHH
HILWTKGTLYLAYTQTNWFQIYNDPQSAPMRMINFMPELIYVYPINFKPFGGKIGNFSEI
HHHHCCCEEEEEEEECCEEEEECCCCCHHHHHHHHCCCEEEEEEECEECCCCCCCCCHHE
WIGWQHISNGVGGAQCYQPFNKEGNPENQFPGGPVIVKDYNGQKDVRWGGCRSVSAGQRP
EEEHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
VFRLVWEKGGLKIMVAYWPYVPYDQSNPNLIDYMGYGNAKIDYRRGRHHFELQLYDIFTQ
EEEEEEECCCCEEEEEECCCCCCCCCCCCEEEEECCCCCEEEECCCCEEEEEEHHHHHHH
YWRYDRWHGAFRLGYTYRINPFVGIYAQWFNGYGDGLYEYDVFSNRIGVGIRLNP
HHCCCCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECC
>Mature Secondary Structure
MKSILLFMIFVVCQLEGKKFSQDNFKVDYNYYLRKQDLHIIKTQNDLSNAWYLPPQKAPK
CHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCEEEEEECCCCCCEEECCCCCCCC
EHSWVDFAKKYLNMMDYLGTYFLPFYHSFTPIFQWYHPNINPYQRNEFKFQISFRVPVFR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCEEEEEEEEECHHHH
HILWTKGTLYLAYTQTNWFQIYNDPQSAPMRMINFMPELIYVYPINFKPFGGKIGNFSEI
HHHHCCCEEEEEEEECCEEEEECCCCCHHHHHHHHCCCEEEEEEECEECCCCCCCCCHHE
WIGWQHISNGVGGAQCYQPFNKEGNPENQFPGGPVIVKDYNGQKDVRWGGCRSVSAGQRP
EEEHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
VFRLVWEKGGLKIMVAYWPYVPYDQSNPNLIDYMGYGNAKIDYRRGRHHFELQLYDIFTQ
EEEEEEECCCCEEEEEECCCCCCCCCCCCEEEEECCCCCEEEECCCCEEEEEEHHHHHHH
YWRYDRWHGAFRLGYTYRINPFVGIYAQWFNGYGDGLYEYDVFSNRIGVGIRLNP
HHCCCCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphatidylcholine; H2O

Specific reaction: phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate

General reaction: Carboxylic ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA