Click here to switch to the map view.

The map label for this gene is ispDF [H]

Identifier: 108562852

GI number: 108562852

Start: 435174

End: 436394

Strand: Reverse

Name: ispDF [H]

Synonym: HPAG1_0427

Alternate gene names: 108562852

Gene position: 436394-435174 (Counterclockwise)

Preceding gene: 108562853

Following gene: 108562851

Centisome position: 27.34

GC content: 40.54

Gene sequence:

>1221_bases
ATGTCTTTGATTAGAGTGAATGGGGAAGCTTTTAAATTCTCTTTAGAAAGTTTAGAAGAAGACCCTTTTGAAACTAAAGA
AACGCTAGAGATTCTTGTTAAACAAACGAGCGTTGTTTTATTGGCCGCTGGGGAATCTAGGCGTTTTTCTCAAACGATTA
AAAAGCAGTGGCTACGCTCTAATCATACCCCCTTATGGCTCAGCGTTTATGAAAGTTTTAAAGAAGCCCTAGACTTTAAA
GAAATCGTTTTAGTCGTAAGCGAATTGGATTATATTTATATCCAACGCCATTACCCTGAAATCAAGCTTATAAAAGGCGG
GGCATCAAGGCAAGAATCCGTGCGTAACGCTTTAAAAATAATTGATAGCGCTTACACGCTCACCAGCGATGTGGCTAGGG
GTTTAGCCAATATGGAAACGCTTAAAAGTTTGTTTTTAACCCTCCAACAAACAAGCCATTATTGCATCGCCCCTTACTTG
CCTTGCTATGACACAGCGATCTATTATAACGAGGCTTTAGACAGAGAGGCGATCAAACTCATTCAAACCCCGCAATTAAG
CCACACTAAAACGCTCCAATCAGCCCTAAATCAAGGGGATTTTAAAGATGAAAGCAGCGCGATTTTACAAGCTTTCCCTA
ACTCTGTGAGCTATATTGAAGGCAGTAAGGATTTGCACAAGCTCACCACAAGCGATGATTTGAAACATTTTGCATTCTTT
TTTAACCCAGCAAAAGACACTTTTATAGGCATGGGCTTTGATACGCATGCGTTCATTAAAGATAAGCCTATGGTTTTAGG
GGGGGTTGTTTTGGATTGCGAGTTTGGGTTAAAGGCCCATAGCGATGGCGATGCTTTGTTGCATGCGGTTATTGATGCGG
TTTTAGGAGCGATTAAAGGGGGGGATATTGGCGAATGGTTCCCTGATAATGACCCCAAATACAAAAACGCCTCTTCTAAA
GAGCTTTTAAAAATCGTGTTGGATTTTTCTCAAAGCATTGGGTTTGAATTGTTTGAAATGGGGGCGACCATCTTTAGCGA
AATCCCTAAAATCACTCCTTACAAACCGGCGATTTTAGAGAATTTGAGCCAACTTTTGGGTTTAGAAAAATCTCAAATCA
GCTTGAAAGCCACTACAATGGAAAAAATGGGGTTCATTGGCAAACAAGAAGGGCTGTTAGTCCAAGCGCATGTGAGCATG
CGTTATAAACAAAAACTTTAA

Upstream 100 bases:

>100_bases
AGAAAAGTATAAAGGCAAACCCAAACGATTCTTAGTTTTAGATTTGAATCAAGGTTATAGGATCATTTTGGTGAAATGAT
AGAGATGGGTTGTTAGTCGC

Downstream 100 bases:

>100_bases
ATGTGATAAGAGGAAGAATTGATGAAAATCTTAATCATTGAAGACGATTTAGCGCTAGCTAGGAGTATTTCGCATAATTT
GCATGATTTGGGGCATTTTT

Product: bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase protein

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSLIRVNGEAFKFSLESLEEDPFETKETLEILVKQTSVVLLAAGESRRFSQTIKKQWLRSNHTPLWLSVYESFKEALDFK
EIVLVVSELDYIYIQRHYPEIKLIKGGASRQESVRNALKIIDSAYTLTSDVARGLANMETLKSLFLTLQQTSHYCIAPYL
PCYDTAIYYNEALDREAIKLIQTPQLSHTKTLQSALNQGDFKDESSAILQAFPNSVSYIEGSKDLHKLTTSDDLKHFAFF
FNPAKDTFIGMGFDTHAFIKDKPMVLGGVVLDCEFGLKAHSDGDALLHAVIDAVLGAIKGGDIGEWFPDNDPKYKNASSK
ELLKIVLDFSQSIGFELFEMGATIFSEIPKITPYKPAILENLSQLLGLEKSQISLKATTMEKMGFIGKQEGLLVQAHVSM
RYKQKL

Sequences:

>Translated_406_residues
MSLIRVNGEAFKFSLESLEEDPFETKETLEILVKQTSVVLLAAGESRRFSQTIKKQWLRSNHTPLWLSVYESFKEALDFK
EIVLVVSELDYIYIQRHYPEIKLIKGGASRQESVRNALKIIDSAYTLTSDVARGLANMETLKSLFLTLQQTSHYCIAPYL
PCYDTAIYYNEALDREAIKLIQTPQLSHTKTLQSALNQGDFKDESSAILQAFPNSVSYIEGSKDLHKLTTSDDLKHFAFF
FNPAKDTFIGMGFDTHAFIKDKPMVLGGVVLDCEFGLKAHSDGDALLHAVIDAVLGAIKGGDIGEWFPDNDPKYKNASSK
ELLKIVLDFSQSIGFELFEMGATIFSEIPKITPYKPAILENLSQLLGLEKSQISLKATTMEKMGFIGKQEGLLVQAHVSM
RYKQKL
>Mature_405_residues
SLIRVNGEAFKFSLESLEEDPFETKETLEILVKQTSVVLLAAGESRRFSQTIKKQWLRSNHTPLWLSVYESFKEALDFKE
IVLVVSELDYIYIQRHYPEIKLIKGGASRQESVRNALKIIDSAYTLTSDVARGLANMETLKSLFLTLQQTSHYCIAPYLP
CYDTAIYYNEALDREAIKLIQTPQLSHTKTLQSALNQGDFKDESSAILQAFPNSVSYIEGSKDLHKLTTSDDLKHFAFFF
NPAKDTFIGMGFDTHAFIKDKPMVLGGVVLDCEFGLKAHSDGDALLHAVIDAVLGAIKGGDIGEWFPDNDPKYKNASSKE
LLKIVLDFSQSIGFELFEMGATIFSEIPKITPYKPAILENLSQLLGLEKSQISLKATTMEKMGFIGKQEGLLVQAHVSMR
YKQKL

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=148, Percent_Identity=45.2702702702703, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 45591; Mature: 45460

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS01295 ISPD ; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIRVNGEAFKFSLESLEEDPFETKETLEILVKQTSVVLLAAGESRRFSQTIKKQWLRS
CCEEEECCHHHEEEHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHC
NHTPLWLSVYESFKEALDFKEIVLVVSELDYIYIQRHYPEIKLIKGGASRQESVRNALKI
CCCCCHHHHHHHHHHHHCHHHHHHHHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHHHH
IDSAYTLTSDVARGLANMETLKSLFLTLQQTSHYCIAPYLPCYDTAIYYNEALDREAIKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHH
IQTPQLSHTKTLQSALNQGDFKDESSAILQAFPNSVSYIEGSKDLHKLTTSDDLKHFAFF
HCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHCCCCCHHHHHCCCCCCHHHHHHE
FNPAKDTFIGMGFDTHAFIKDKPMVLGGVVLDCEFGLKAHSDGDALLHAVIDAVLGAIKG
ECCCCCCEEECCCCCCEEECCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCC
GDIGEWFPDNDPKYKNASSKELLKIVLDFSQSIGFELFEMGATIFSEIPKITPYKPAILE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHH
NLSQLLGLEKSQISLKATTMEKMGFIGKQEGLLVQAHVSMRYKQKL
HHHHHHCCCHHHEEEEEHHHHHHCCCCCCCCEEEEEEHHHHHHHCC
>Mature Secondary Structure 
SLIRVNGEAFKFSLESLEEDPFETKETLEILVKQTSVVLLAAGESRRFSQTIKKQWLRS
CEEEECCHHHEEEHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHC
NHTPLWLSVYESFKEALDFKEIVLVVSELDYIYIQRHYPEIKLIKGGASRQESVRNALKI
CCCCCHHHHHHHHHHHHCHHHHHHHHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHHHH
IDSAYTLTSDVARGLANMETLKSLFLTLQQTSHYCIAPYLPCYDTAIYYNEALDREAIKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHH
IQTPQLSHTKTLQSALNQGDFKDESSAILQAFPNSVSYIEGSKDLHKLTTSDDLKHFAFF
HCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHCCCCCHHHHHCCCCCCHHHHHHE
FNPAKDTFIGMGFDTHAFIKDKPMVLGGVVLDCEFGLKAHSDGDALLHAVIDAVLGAIKG
ECCCCCCEEECCCCCCEEECCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCC
GDIGEWFPDNDPKYKNASSKELLKIVLDFSQSIGFELFEMGATIFSEIPKITPYKPAILE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHH
NLSQLLGLEKSQISLKATTMEKMGFIGKQEGLLVQAHVSMRYKQKL
HHHHHHCCCHHHEEEEEHHHHHHCCCCCCCCEEEEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]