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The map label for this gene is kgtP [H]

Identifier: 108562781

GI number: 108562781

Start: 364832

End: 366112

Strand: Reverse

Name: kgtP [H]

Synonym: HPAG1_0356

Alternate gene names: 108562781

Gene position: 366112-364832 (Counterclockwise)

Preceding gene: 108562782

Following gene: 108562780

Centisome position: 22.93

GC content: 40.05

Gene sequence:

>1281_bases
ATGAACCCCCAACCCGCCACCAAAAAACCCTTAAAATCCCTTTTAGCCGCTAGTTCAGGTAATTTAGTGGAATGGTATGA
CTTTTACGCTTATGCGTTCCTCGCTCCTTATTTTGCTAAGGAATTTACCCACACCAATGACCCTACTTTAGCGCTAATCT
CAGCTTTTTTGGTTTTTATGCTAGGGTTTTTCATGCGCCCTTTGGGGAGTTTGTTTTTTGGTAAATTGGGGGATAAAAAG
GGGCGTAAAACTTCCATGGTGTATTCCATTATCCTTATGGCGCTAGGCTCTTTTTTGCTCGCATTGCTCCCCACTAAAGA
AATCGTAGGAGAATGGGCGTTCTTGTTTTTATTATTAGCAAGGCTTTTACAAGGCTTTAGCGTGGGAGGAGAATATGGCG
TGGTCGCTACTTACCTCTCTGAATTAGGCAAGAATGGCAAAAAAGGTTTTTATGGCTCTTTTCAATATGTAACTTTAGTT
GGAGGGCAACTCTTAGCCATTTTTTCGCTCTTTATCGTTGAAAATATTTACACGCATGAACAAATCAGCGCGTTCGCTTG
GCGTTATTTATTCGCTTTAGGGGGTATATTAGCCCTACTCTCGCTCTTTTTAAGAAATATCATGGAAGAAACCATGGATA
GCAAAACAACTTCCAAAACCACTATTAAAGAAGAAACCCAAAGAGGCAGTTTAAAGGAATTGCTCCACCATAAAAAAGCC
TTAATGATAGTTTTTGGACTCACTATTGGAGGGAGTTTGTGTTTTTATACTTTTACGGTATATTTAAAAATCTTTTTAAC
CAACAGCTCATCATTCAGCCCTAAAGAAAGCAGTTTTATCATGCTTTTATCGCTCACTTATTTCATCTTCTTACAGCCCT
TATGCGGGATGCTTGCGGATAAAATCAAACGCACCCAAATGTTGATGGTTTTTGCTATCACAGGGCTTATTGTAACGCCT
GTTGTCTTTTATGGTATCAAGCATGCCACTAGCGTGTATGAAGCCCTATTTTATGAAATACTCGCATTGAGCAGCATGAG
TTTTTACACTTGCATTGCTGGGGTCATTAAAGCGGAATTGTTCCCTGAACATGTGCGAGCACTTGGCGTGGGTTTAGCCT
ATGCGATTGCGAATGCGCTTTTTGGAGGGAGCGCGAGTTATGTAGCGTTAGAGTTCAAACAACATGGTTTTGAATGGGGG
TTTGTGGGCTATGTCATGTTTAGTATTGTTATCTTTATGGTTATGGTTATCATATTCCCTAAAAAAACCTATTTGGAATG
A

Upstream 100 bases:

>100_bases
ATACAAACGACACCAACCTTTCATCTATTAATCAAATATTATCTGTGGTTTCCCTAACTCCTAGTAAAATTACCTAAAAA
TTTCATATTCAAGGATAGCC

Downstream 100 bases:

>100_bases
ATCTGTTTTTATTATAATTTTTGCTATAATTTTTCCTTTTAAAAGGATTCTTGCATGCAAAATGGGTATTATGCAGCCAC
GGGAGCTATGGCTACACAAT

Product: alpha-ketoglutarate permease

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MNPQPATKKPLKSLLAASSGNLVEWYDFYAYAFLAPYFAKEFTHTNDPTLALISAFLVFMLGFFMRPLGSLFFGKLGDKK
GRKTSMVYSIILMALGSFLLALLPTKEIVGEWAFLFLLLARLLQGFSVGGEYGVVATYLSELGKNGKKGFYGSFQYVTLV
GGQLLAIFSLFIVENIYTHEQISAFAWRYLFALGGILALLSLFLRNIMEETMDSKTTSKTTIKEETQRGSLKELLHHKKA
LMIVFGLTIGGSLCFYTFTVYLKIFLTNSSSFSPKESSFIMLLSLTYFIFLQPLCGMLADKIKRTQMLMVFAITGLIVTP
VVFYGIKHATSVYEALFYEILALSSMSFYTCIAGVIKAELFPEHVRALGVGLAYAIANALFGGSASYVALEFKQHGFEWG
FVGYVMFSIVIFMVMVIIFPKKTYLE

Sequences:

>Translated_426_residues
MNPQPATKKPLKSLLAASSGNLVEWYDFYAYAFLAPYFAKEFTHTNDPTLALISAFLVFMLGFFMRPLGSLFFGKLGDKK
GRKTSMVYSIILMALGSFLLALLPTKEIVGEWAFLFLLLARLLQGFSVGGEYGVVATYLSELGKNGKKGFYGSFQYVTLV
GGQLLAIFSLFIVENIYTHEQISAFAWRYLFALGGILALLSLFLRNIMEETMDSKTTSKTTIKEETQRGSLKELLHHKKA
LMIVFGLTIGGSLCFYTFTVYLKIFLTNSSSFSPKESSFIMLLSLTYFIFLQPLCGMLADKIKRTQMLMVFAITGLIVTP
VVFYGIKHATSVYEALFYEILALSSMSFYTCIAGVIKAELFPEHVRALGVGLAYAIANALFGGSASYVALEFKQHGFEWG
FVGYVMFSIVIFMVMVIIFPKKTYLE
>Mature_426_residues
MNPQPATKKPLKSLLAASSGNLVEWYDFYAYAFLAPYFAKEFTHTNDPTLALISAFLVFMLGFFMRPLGSLFFGKLGDKK
GRKTSMVYSIILMALGSFLLALLPTKEIVGEWAFLFLLLARLLQGFSVGGEYGVVATYLSELGKNGKKGFYGSFQYVTLV
GGQLLAIFSLFIVENIYTHEQISAFAWRYLFALGGILALLSLFLRNIMEETMDSKTTSKTTIKEETQRGSLKELLHHKKA
LMIVFGLTIGGSLCFYTFTVYLKIFLTNSSSFSPKESSFIMLLSLTYFIFLQPLCGMLADKIKRTQMLMVFAITGLIVTP
VVFYGIKHATSVYEALFYEILALSSMSFYTCIAGVIKAELFPEHVRALGVGLAYAIANALFGGSASYVALEFKQHGFEWG
FVGYVMFSIVIFMVMVIIFPKKTYLE

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=416, Percent_Identity=45.4326923076923, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1790550, Length=376, Percent_Identity=28.7234042553192, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1788292, Length=385, Percent_Identity=28.3116883116883, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789941, Length=399, Percent_Identity=29.0726817042606, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87082404, Length=432, Percent_Identity=25, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87082231, Length=214, Percent_Identity=28.9719626168224, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47575; Mature: 47575

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHCC
>Mature Secondary Structure
MNPQPATKKPLKSLLAASSGNLVEWYDFYAYAFLAPYFAKEFTHTNDPTLALISAFLVFM
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LGFFMRPLGSLFFGKLGDKKGRKTSMVYSIILMALGSFLLALLPTKEIVGEWAFLFLLLA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
RLLQGFSVGGEYGVVATYLSELGKNGKKGFYGSFQYVTLVGGQLLAIFSLFIVENIYTHE
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QISAFAWRYLFALGGILALLSLFLRNIMEETMDSKTTSKTTIKEETQRGSLKELLHHKKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHH
LMIVFGLTIGGSLCFYTFTVYLKIFLTNSSSFSPKESSFIMLLSLTYFIFLQPLCGMLAD
HHHHHHHHHCCHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KIKRTQMLMVFAITGLIVTPVVFYGIKHATSVYEALFYEILALSSMSFYTCIAGVIKAEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
FPEHVRALGVGLAYAIANALFGGSASYVALEFKQHGFEWGFVGYVMFSIVIFMVMVIIFP
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
KKTYLE
CHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]