Click here to switch to the map view.

The map label for this gene is pyrG

Identifier: 108562769

GI number: 108562769

Start: 352521

End: 354137

Strand: Reverse

Name: pyrG

Synonym: HPAG1_0344

Alternate gene names: 108562769

Gene position: 354137-352521 (Counterclockwise)

Preceding gene: 108562778

Following gene: 108562768

Centisome position: 22.18

GC content: 42.12

Gene sequence:

>1617_bases
ATGGATAGAGCCAAATTTATATTCGTTACAGGGGGCGTGTTAAGCTCTCTAGGGAAAGGGATTTCATCTTCTTCAATCGC
TACGCTTTTACAGCATTGCAATTACCAGGTTTCTATTTTGAAGATTGACCCTTATATCAATATTGATCCAGGCACCATGA
GCCCTTTAGAGCATGGGGAAGTGTTTGTTACTAGCGATGGCGCTGAAACGGATTTAGACATAGGGCATTATGAACGCTTT
TTGAACAGGAATTTAACCAGGTTGAATAATTTCACTACCGGGCAGATTTTTTCGAGCGTGATAGAAAATGAAAGGAAAGG
GGAATATTTAGGCAAAACCATTCAAATCGTCCCCCATGTAACCGATGAAATCAAAAGGCGCATTAAAAGTGCGGCTAAGG
GGTTGGATTTTTTAATCGTGGAAGTGGGCGGAACCGTGGGCGATATGGAGGGCATGTTTTATTTGGAAGCGATCCGCCAG
CTTAAATTGGAATTAGGGAATGAAAAAGTCATTAATGTGCATGTAACCTTGATCCCCTATATCCAAACCACTAACGAGCT
AAAAACCAAACCCACGCAACATTCCGTTCAAGAATTACGGCGCCTTGGCGTAACCCCTCAAATCATTTTGGCGCGATCGC
CTAAGCCTTTGGATAAAGAATTGAAAAATAAAATCGCTTTGAGTTGCGATGTGGAGCAAGACAGCGTGATTGTCGCCACA
GACACTAAAAGCATTTACGCATGCCCCATTCTTTTCTTGCAAGAAGGCATTTTAACCCCCATTGCCAGACGCTTTAATTT
GAATAAACTACACCCCAAAATGGCGGCTTGGAACACTTTAGTAGAAAAGATTATCGCTCCTAAACACAAAGTCAAAATTG
GTTTTGTGGGCAAGTATTTAAGCTTGAAAGAATCTTATAAATCCTTGATTGAAGCCTTAATCCATGCGGGTGCGCATTTG
GATGCGCAAGTCAATATTGAATGGTTGGATAGCGAGAATTTTAATGAAAAGACGGATTTAGAGGGCGTTGATGCGATTTT
AGTGCCTGGAGGCTTTGGAGAAAGGGGGATTGAGGGCAAAATTTGCGCCATTCAAAGAGCTAGGTTAGAAAAACTTCCTT
TTTTAGGGATTTGTTTAGGCATGCAATTAGCGATCGTTGAATTTTGCCGCAATGTTTTGGGTTTAAAAGGGGCTAACTCT
ACGGAGTTTAACCAACGCTGCGAATACCCTGTGGTGTATTTGATTGGAGATTTTATGGATCAAAACCACCAAAAACAGGT
GCGTACCTATAATTCGCCATTGGGTGGCACCATGCGATTAGGCGAATACGAATGCGAAATCATGCCCAACAGCTTGCTAG
AAAAAGCTTATAAAAAGCCTAGCATTAAAGAAAGACACCGCCATCGTTATGAAATCAACCCCAAATACCGCCAAGAGTGG
GAAAGTAAGGGCTTGAAAGTGGTGGGTTTTGGGGCGAATCATTTGATTGAAGCGATTGAATTAGAAGATCACCCGTTCTT
TGTGGGGGTGCAATTCCACCCAGAATTCACCTCCAGGTTGCAAAGCCCTAACCCTATTATTTTGGATTTCATTAAGAGCG
CTCTTTCTAAATCCTAA

Upstream 100 bases:

>100_bases
TTTTTTTGAAATCGCTTTAATTTTTGCCCTCTTTTAAAGCTTTTTAACCCTTTTTAGACTACAATTTTAAGAAGTAAAAA
TCAAAGGAACGCAATAACTT

Downstream 100 bases:

>100_bases
TCTAAAAGAATGAAACAAAAGCTTAAAGCTCAGATCAAAGAGCGCATGGCTTCTATCGCTTATAATGAAAAAGGGTTTCC
TAGCCCCTTTTTATTTAAAG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MDRAKFIFVTGGVLSSLGKGISSSSIATLLQHCNYQVSILKIDPYINIDPGTMSPLEHGEVFVTSDGAETDLDIGHYERF
LNRNLTRLNNFTTGQIFSSVIENERKGEYLGKTIQIVPHVTDEIKRRIKSAAKGLDFLIVEVGGTVGDMEGMFYLEAIRQ
LKLELGNEKVINVHVTLIPYIQTTNELKTKPTQHSVQELRRLGVTPQIILARSPKPLDKELKNKIALSCDVEQDSVIVAT
DTKSIYACPILFLQEGILTPIARRFNLNKLHPKMAAWNTLVEKIIAPKHKVKIGFVGKYLSLKESYKSLIEALIHAGAHL
DAQVNIEWLDSENFNEKTDLEGVDAILVPGGFGERGIEGKICAIQRARLEKLPFLGICLGMQLAIVEFCRNVLGLKGANS
TEFNQRCEYPVVYLIGDFMDQNHQKQVRTYNSPLGGTMRLGEYECEIMPNSLLEKAYKKPSIKERHRHRYEINPKYRQEW
ESKGLKVVGFGANHLIEAIELEDHPFFVGVQFHPEFTSRLQSPNPIILDFIKSALSKS

Sequences:

>Translated_538_residues
MDRAKFIFVTGGVLSSLGKGISSSSIATLLQHCNYQVSILKIDPYINIDPGTMSPLEHGEVFVTSDGAETDLDIGHYERF
LNRNLTRLNNFTTGQIFSSVIENERKGEYLGKTIQIVPHVTDEIKRRIKSAAKGLDFLIVEVGGTVGDMEGMFYLEAIRQ
LKLELGNEKVINVHVTLIPYIQTTNELKTKPTQHSVQELRRLGVTPQIILARSPKPLDKELKNKIALSCDVEQDSVIVAT
DTKSIYACPILFLQEGILTPIARRFNLNKLHPKMAAWNTLVEKIIAPKHKVKIGFVGKYLSLKESYKSLIEALIHAGAHL
DAQVNIEWLDSENFNEKTDLEGVDAILVPGGFGERGIEGKICAIQRARLEKLPFLGICLGMQLAIVEFCRNVLGLKGANS
TEFNQRCEYPVVYLIGDFMDQNHQKQVRTYNSPLGGTMRLGEYECEIMPNSLLEKAYKKPSIKERHRHRYEINPKYRQEW
ESKGLKVVGFGANHLIEAIELEDHPFFVGVQFHPEFTSRLQSPNPIILDFIKSALSKS
>Mature_538_residues
MDRAKFIFVTGGVLSSLGKGISSSSIATLLQHCNYQVSILKIDPYINIDPGTMSPLEHGEVFVTSDGAETDLDIGHYERF
LNRNLTRLNNFTTGQIFSSVIENERKGEYLGKTIQIVPHVTDEIKRRIKSAAKGLDFLIVEVGGTVGDMEGMFYLEAIRQ
LKLELGNEKVINVHVTLIPYIQTTNELKTKPTQHSVQELRRLGVTPQIILARSPKPLDKELKNKIALSCDVEQDSVIVAT
DTKSIYACPILFLQEGILTPIARRFNLNKLHPKMAAWNTLVEKIIAPKHKVKIGFVGKYLSLKESYKSLIEALIHAGAHL
DAQVNIEWLDSENFNEKTDLEGVDAILVPGGFGERGIEGKICAIQRARLEKLPFLGICLGMQLAIVEFCRNVLGLKGANS
TEFNQRCEYPVVYLIGDFMDQNHQKQVRTYNSPLGGTMRLGEYECEIMPNSLLEKAYKKPSIKERHRHRYEINPKYRQEW
ESKGLKVVGFGANHLIEAIELEDHPFFVGVQFHPEFTSRLQSPNPIILDFIKSALSKS

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=560, Percent_Identity=44.6428571428571, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI28559085, Length=557, Percent_Identity=43.4470377019749, Blast_Score=454, Evalue=1e-128,
Organism=Homo sapiens, GI28559083, Length=557, Percent_Identity=43.4470377019749, Blast_Score=454, Evalue=1e-128,
Organism=Homo sapiens, GI221316689, Length=557, Percent_Identity=43.4470377019749, Blast_Score=454, Evalue=1e-128,
Organism=Escherichia coli, GI1789142, Length=532, Percent_Identity=49.0601503759398, Blast_Score=541, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI25148299, Length=609, Percent_Identity=38.5878489326765, Blast_Score=419, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6322563, Length=571, Percent_Identity=41.1558669001751, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319432, Length=571, Percent_Identity=40.2802101576182, Blast_Score=409, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24664469, Length=560, Percent_Identity=44.1071428571429, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI21357815, Length=505, Percent_Identity=42.1782178217822, Blast_Score=374, Evalue=1e-103,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_HELPH (Q1CUG1)

Other databases:

- EMBL:   CP000241
- RefSeq:   YP_627085.1
- ProteinModelPortal:   Q1CUG1
- SMR:   Q1CUG1
- STRING:   Q1CUG1
- GeneID:   4099353
- GenomeReviews:   CP000241_GR
- KEGG:   hpa:HPAG1_0344
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- BioCyc:   HPYL357544:HPAG1_0344-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60544; Mature: 60544

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 378-378 ACT_SITE 513-513 ACT_SITE 515-515

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRAKFIFVTGGVLSSLGKGISSSSIATLLQHCNYQVSILKIDPYINIDPGTMSPLEHGE
CCCCEEEEEECHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCCCCCCC
VFVTSDGAETDLDIGHYERFLNRNLTRLNNFTTGQIFSSVIENERKGEYLGKTIQIVPHV
EEEECCCCCCCCCHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCHHCCEEEEECCC
TDEIKRRIKSAAKGLDFLIVEVGGTVGDMEGMFYLEAIRQLKLELGNEKVINVHVTLIPY
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEE
IQTTNELKTKPTQHSVQELRRLGVTPQIILARSPKPLDKELKNKIALSCDVEQDSVIVAT
EECCCHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCEEEEEECCCCCEEEEE
DTKSIYACPILFLQEGILTPIARRFNLNKLHPKMAAWNTLVEKIIAPKHKVKIGFVGKYL
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHH
SLKESYKSLIEALIHAGAHLDAQVNIEWLDSENFNEKTDLEGVDAILVPGGFGERGIEGK
HHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
ICAIQRARLEKLPFLGICLGMQLAIVEFCRNVLGLKGANSTEFNQRCEYPVVYLIGDFMD
EEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEEEHHHC
QNHQKQVRTYNSPLGGTMRLGEYECEIMPNSLLEKAYKKPSIKERHRHRYEINPKYRQEW
CCCHHHHHHCCCCCCCCEEECCEEEEECCHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHH
ESKGLKVVGFGANHLIEAIELEDHPFFVGVQFHPEFTSRLQSPNPIILDFIKSALSKS
HHCCCEEEEECHHHHHEEEEECCCCEEEEEEECCHHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MDRAKFIFVTGGVLSSLGKGISSSSIATLLQHCNYQVSILKIDPYINIDPGTMSPLEHGE
CCCCEEEEEECHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCCCCCCC
VFVTSDGAETDLDIGHYERFLNRNLTRLNNFTTGQIFSSVIENERKGEYLGKTIQIVPHV
EEEECCCCCCCCCHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCHHCCEEEEECCC
TDEIKRRIKSAAKGLDFLIVEVGGTVGDMEGMFYLEAIRQLKLELGNEKVINVHVTLIPY
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEE
IQTTNELKTKPTQHSVQELRRLGVTPQIILARSPKPLDKELKNKIALSCDVEQDSVIVAT
EECCCHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCEEEEEECCCCCEEEEE
DTKSIYACPILFLQEGILTPIARRFNLNKLHPKMAAWNTLVEKIIAPKHKVKIGFVGKYL
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHH
SLKESYKSLIEALIHAGAHLDAQVNIEWLDSENFNEKTDLEGVDAILVPGGFGERGIEGK
HHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
ICAIQRARLEKLPFLGICLGMQLAIVEFCRNVLGLKGANSTEFNQRCEYPVVYLIGDFMD
EEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEEEHHHC
QNHQKQVRTYNSPLGGTMRLGEYECEIMPNSLLEKAYKKPSIKERHRHRYEINPKYRQEW
CCCHHHHHHCCCCCCCCEEECCEEEEECCHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHH
ESKGLKVVGFGANHLIEAIELEDHPFFVGVQFHPEFTSRLQSPNPIILDFIKSALSKS
HHCCCEEEEECHHHHHEEEEECCCCEEEEEEECCHHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA