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The map label for this gene is thrS [H]

Identifier: 108562547

GI number: 108562547

Start: 130909

End: 132747

Strand: Direct

Name: thrS [H]

Synonym: HPAG1_0122

Alternate gene names: 108562547

Gene position: 130909-132747 (Clockwise)

Preceding gene: 108562546

Following gene: 108562548

Centisome position: 8.2

GC content: 41.44

Gene sequence:

>1839_bases
ATGAGTGCGGAACTGATTGCTGTTTATAAAGACGAGCAAATAATAGATTTAGAGAGCGCGAAAGTCTTAGGGCTGAGCGA
TGGGATTAAAGCGTTAAACGGGACAGAGCCGATATATTTTGATGATTCGCCTTTGGCTTTAGAAGTGATCAGGCATTCAT
GCGCGCATTTGCTCGCGCAAAGCTTGAAAGCCCTTTATCCGGACGCAAAATTTTTTGTAGGCCCTGTGGTAGAAGAGGGG
TTTTATTACGATTTTAAGACCGCTTCAAAAATCAGCGAAGAGGATTTGCCTAAAATTGAAGCGAAAATGAAAGAGTTTGC
GAAGTCAAAGCTCGCTATCACTAAAGAGACTTTAACCAGAGAGCAAGCTTTGGAGCGTTTTAAGGGCGATGAATTAAAGC
ATGCGGTGATGAGTAAAATCAGTGGCGATATATTTGGCGTGTATAAACAAGGCGAGTTTGAAGATTTGTGTAAGGGGCCG
CACCTCCCAAACACCCGTTTTTTAAACCATTTCAAGCTCACTAAACTGGCTGGGGCTTATTTGGGCGGCGATGAAAACAA
TGAAATGCTCATTAGAATCTATGGCATCGCTTTTGCCACCAAAGAGGGCTTAAAAGATTATCTTTTCCAAATAGAAGAAG
CGAAAAAACGAGATCACAGAAAGCTAGGCGTGGAGCTAGGGCTCTTTAGTTTTGATGATGAGATAGGGGCGGGCTTACCT
TTATGGCTGCCTAAAGGGGCAAGACTCAGAAAACGCATTGAAGATTTATTGAGTCAAGCGTTACTTTTAAGAGGCTATGA
GCCGGTTAAAGGCCCTGAGATTTTAAAGAGCGATGTGTGGAAAATCAGCGGGCATTATGACAACTATAAAGAAAACATGT
ATTTCACCACGATTGATGAGCAAGAATACGGCATAAAGCCTATGAATTGCGTGGGGCATATTAAAGTCTATCAAAGCGCT
TTACACAGCTATAGAGATTTGCCTTTAAGGTTTTATGAATACGGCGTGGTGCATCGGCATGAAAAAAGCGGCGTGTTGCA
TGGGCTTTTAAGGGTTAGGGAATTTACCCAAGATGATGCACATATTTTTTGCTCTTTTGAACAGATCCAAAGCGAAGTGA
GCGCGATTTTAGATTTCACGCATAAGATCATGCAAGCGTTTGATTTTAGCTATGAAATGGAATTATCCACTAGACCGGCT
AAATCCATAGGCGATGATGAAGTTTGGGAAAAGGCCACTAACGCTTTAAAAGAAGCCCTAAAAGAGCACCGCATTGATTA
TAAGATTGATGAAGGGGGAGGGGCTTTCTATGGGCCTAAGATTGACATTAAAATCACTGACGCTTTAAGGCGTAAATGGC
AGTGCGGCACGATTCAAGTGGATATGAATTTGCCTGAACGCTTCAAACTCGCTTTCACTAATGAGCGCAATCACGCTGAG
CAGCCGGTGATGATCCACAGAGCGATTTTAGGCTCGTTTGAAAGGTTTATTGCGATTTTGAGCGAACATTTTGGAGGGAA
TTTCCCTTTCTTTGTCGCGCCCACTCAAATCGCTCTCATTCCTATTAATGAAGAGCATCATGTTTTTGCTTTGAAATTAA
AAGAGGAACTAAAAAAGCGCGATATTTTTGTAGAAGTGCTGGATAAAAACGACAGCTTGAATAAAAAGGTGCGATTAGCC
GAAAAGCAAAAAATCCCTATGATTTTAGTGCTAGGGAATGAGGAAGTGGAGACCGAAATTTTATCCATTAGAGACAGAGA
AAAACAAGCTCAATATAAAATGCCCTTAAAGGAGTTTTTAAACATGGTTGAATCTAAGATGCAAGAGGTTAGTTTTTGA

Upstream 100 bases:

>100_bases
TGGATTCGCAAGTGCAATGCGGATTTGGCTCAAAAGATTGCTAAAAGCATTGAAAAAATATTGAAAAAGTATTAGAGAAA
GGAAACATAAGTTTTAAAGA

Downstream 100 bases:

>100_bases
GTAGAAACGAAGTGTTGTTAAACGGAGACATTAATTTTAAAGAAGTGCGTTGCGTGGGCGATAATGGCGAAGTGTATGGG
ATCATTTCTTCCAAAGAAGC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MSAELIAVYKDEQIIDLESAKVLGLSDGIKALNGTEPIYFDDSPLALEVIRHSCAHLLAQSLKALYPDAKFFVGPVVEEG
FYYDFKTASKISEEDLPKIEAKMKEFAKSKLAITKETLTREQALERFKGDELKHAVMSKISGDIFGVYKQGEFEDLCKGP
HLPNTRFLNHFKLTKLAGAYLGGDENNEMLIRIYGIAFATKEGLKDYLFQIEEAKKRDHRKLGVELGLFSFDDEIGAGLP
LWLPKGARLRKRIEDLLSQALLLRGYEPVKGPEILKSDVWKISGHYDNYKENMYFTTIDEQEYGIKPMNCVGHIKVYQSA
LHSYRDLPLRFYEYGVVHRHEKSGVLHGLLRVREFTQDDAHIFCSFEQIQSEVSAILDFTHKIMQAFDFSYEMELSTRPA
KSIGDDEVWEKATNALKEALKEHRIDYKIDEGGGAFYGPKIDIKITDALRRKWQCGTIQVDMNLPERFKLAFTNERNHAE
QPVMIHRAILGSFERFIAILSEHFGGNFPFFVAPTQIALIPINEEHHVFALKLKEELKKRDIFVEVLDKNDSLNKKVRLA
EKQKIPMILVLGNEEVETEILSIRDREKQAQYKMPLKEFLNMVESKMQEVSF

Sequences:

>Translated_612_residues
MSAELIAVYKDEQIIDLESAKVLGLSDGIKALNGTEPIYFDDSPLALEVIRHSCAHLLAQSLKALYPDAKFFVGPVVEEG
FYYDFKTASKISEEDLPKIEAKMKEFAKSKLAITKETLTREQALERFKGDELKHAVMSKISGDIFGVYKQGEFEDLCKGP
HLPNTRFLNHFKLTKLAGAYLGGDENNEMLIRIYGIAFATKEGLKDYLFQIEEAKKRDHRKLGVELGLFSFDDEIGAGLP
LWLPKGARLRKRIEDLLSQALLLRGYEPVKGPEILKSDVWKISGHYDNYKENMYFTTIDEQEYGIKPMNCVGHIKVYQSA
LHSYRDLPLRFYEYGVVHRHEKSGVLHGLLRVREFTQDDAHIFCSFEQIQSEVSAILDFTHKIMQAFDFSYEMELSTRPA
KSIGDDEVWEKATNALKEALKEHRIDYKIDEGGGAFYGPKIDIKITDALRRKWQCGTIQVDMNLPERFKLAFTNERNHAE
QPVMIHRAILGSFERFIAILSEHFGGNFPFFVAPTQIALIPINEEHHVFALKLKEELKKRDIFVEVLDKNDSLNKKVRLA
EKQKIPMILVLGNEEVETEILSIRDREKQAQYKMPLKEFLNMVESKMQEVSF
>Mature_611_residues
SAELIAVYKDEQIIDLESAKVLGLSDGIKALNGTEPIYFDDSPLALEVIRHSCAHLLAQSLKALYPDAKFFVGPVVEEGF
YYDFKTASKISEEDLPKIEAKMKEFAKSKLAITKETLTREQALERFKGDELKHAVMSKISGDIFGVYKQGEFEDLCKGPH
LPNTRFLNHFKLTKLAGAYLGGDENNEMLIRIYGIAFATKEGLKDYLFQIEEAKKRDHRKLGVELGLFSFDDEIGAGLPL
WLPKGARLRKRIEDLLSQALLLRGYEPVKGPEILKSDVWKISGHYDNYKENMYFTTIDEQEYGIKPMNCVGHIKVYQSAL
HSYRDLPLRFYEYGVVHRHEKSGVLHGLLRVREFTQDDAHIFCSFEQIQSEVSAILDFTHKIMQAFDFSYEMELSTRPAK
SIGDDEVWEKATNALKEALKEHRIDYKIDEGGGAFYGPKIDIKITDALRRKWQCGTIQVDMNLPERFKLAFTNERNHAEQ
PVMIHRAILGSFERFIAILSEHFGGNFPFFVAPTQIALIPINEEHHVFALKLKEELKKRDIFVEVLDKNDSLNKKVRLAE
KQKIPMILVLGNEEVETEILSIRDREKQAQYKMPLKEFLNMVESKMQEVSF

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=593, Percent_Identity=37.6053962900506, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI38202255, Length=563, Percent_Identity=38.0106571936057, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI20070344, Length=577, Percent_Identity=34.315424610052, Blast_Score=357, Evalue=2e-98,
Organism=Escherichia coli, GI1788013, Length=572, Percent_Identity=42.1328671328671, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1786392, Length=160, Percent_Identity=28.125, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71984192, Length=570, Percent_Identity=38.7719298245614, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71984184, Length=570, Percent_Identity=38.7719298245614, Blast_Score=419, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6322112, Length=560, Percent_Identity=40.5357142857143, Blast_Score=436, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6322654, Length=391, Percent_Identity=37.5959079283887, Blast_Score=263, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24583842, Length=603, Percent_Identity=37.4792703150912, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI28574141, Length=603, Percent_Identity=37.4792703150912, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24583839, Length=603, Percent_Identity=37.4792703150912, Blast_Score=425, Evalue=1e-119,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 70307; Mature: 70176

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAELIAVYKDEQIIDLESAKVLGLSDGIKALNGTEPIYFDDSPLALEVIRHSCAHLLAQ
CCCCEEEEECCCEEEEECCCEEEECCCCHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHH
SLKALYPDAKFFVGPVVEEGFYYDFKTASKISEEDLPKIEAKMKEFAKSKLAITKETLTR
HHHHHCCCCHHEECHHHHCCEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EQALERFKGDELKHAVMSKISGDIFGVYKQGEFEDLCKGPHLPNTRFLNHFKLTKLAGAY
HHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LGGDENNEMLIRIYGIAFATKEGLKDYLFQIEEAKKRDHRKLGVELGLFSFDDEIGAGLP
CCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCC
LWLPKGARLRKRIEDLLSQALLLRGYEPVKGPEILKSDVWKISGHYDNYKENMYFTTIDE
EECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCEEEECCCCCCCCCEEEEEECC
QEYGIKPMNCVGHIKVYQSALHSYRDLPLRFYEYGVVHRHEKSGVLHGLLRVREFTQDDA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEECCHHHHHHHHHHHHHHCCCCC
HIFCSFEQIQSEVSAILDFTHKIMQAFDFSYEMELSTRPAKSIGDDEVWEKATNALKEAL
EEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
KEHRIDYKIDEGGGAFYGPKIDIKITDALRRKWQCGTIQVDMNLPERFKLAFTNERNHAE
HHCCCCEEEECCCCEEECCEEEEEEEHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCCC
QPVMIHRAILGSFERFIAILSEHFGGNFPFFVAPTQIALIPINEEHHVFALKLKEELKKR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEECCCCCEEEEEHHHHHHHH
DIFVEVLDKNDSLNKKVRLAEKQKIPMILVLGNEEVETEILSIRDREKQAQYKMPLKEFL
CEEEEEECCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHH
NMVESKMQEVSF
HHHHHHHHHCCC
>Mature Secondary Structure 
SAELIAVYKDEQIIDLESAKVLGLSDGIKALNGTEPIYFDDSPLALEVIRHSCAHLLAQ
CCCEEEEECCCEEEEECCCEEEECCCCHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHH
SLKALYPDAKFFVGPVVEEGFYYDFKTASKISEEDLPKIEAKMKEFAKSKLAITKETLTR
HHHHHCCCCHHEECHHHHCCEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EQALERFKGDELKHAVMSKISGDIFGVYKQGEFEDLCKGPHLPNTRFLNHFKLTKLAGAY
HHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LGGDENNEMLIRIYGIAFATKEGLKDYLFQIEEAKKRDHRKLGVELGLFSFDDEIGAGLP
CCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCC
LWLPKGARLRKRIEDLLSQALLLRGYEPVKGPEILKSDVWKISGHYDNYKENMYFTTIDE
EECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCEEEECCCCCCCCCEEEEEECC
QEYGIKPMNCVGHIKVYQSALHSYRDLPLRFYEYGVVHRHEKSGVLHGLLRVREFTQDDA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEECCHHHHHHHHHHHHHHCCCCC
HIFCSFEQIQSEVSAILDFTHKIMQAFDFSYEMELSTRPAKSIGDDEVWEKATNALKEAL
EEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
KEHRIDYKIDEGGGAFYGPKIDIKITDALRRKWQCGTIQVDMNLPERFKLAFTNERNHAE
HHCCCCEEEECCCCEEECCEEEEEEEHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCCC
QPVMIHRAILGSFERFIAILSEHFGGNFPFFVAPTQIALIPINEEHHVFALKLKEELKKR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEECCCCCEEEEEHHHHHHHH
DIFVEVLDKNDSLNKKVRLAEKQKIPMILVLGNEEVETEILSIRDREKQAQYKMPLKEFL
CEEEEEECCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHH
NMVESKMQEVSF
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA