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The map label for this gene is wcaE [C]

Identifier: 108562527

GI number: 108562527

Start: 105752

End: 106531

Strand: Reverse

Name: wcaE [C]

Synonym: HPAG1_0102

Alternate gene names: 108562527

Gene position: 106531-105752 (Counterclockwise)

Preceding gene: 108562528

Following gene: 108562523

Centisome position: 6.67

GC content: 38.72

Gene sequence:

>780_bases
TTGCTAAAAGTTTCTGTGATCACGGCGTGTTTTAATAGCGAAAAAACCATTGAAGACACCATTCTTTCCGTGCTTAATCA
AACTTATAAAAACATTGAATACATTATTATAGATGGGGCTAGCGCGGATAGCACTTTAGAAATCATTCAAAAATATAGAG
ATAGAATCGCTTGCGTGGTGAGTGAAAAAGATGAGGGCATTTATGACGCTATGAATAAGGGCATAAAGCGTTCTAGTGGG
GATATTATCGCTTTATTGAATAGCGATGATTTTTACAAAGACGAGTTTGTGGTAGAAAAAGTGGTGCATGAATTTGAAAA
CAAAAATTGCGATAGCGTGTATGCGGATCTGGTGTTTGTCAAACCGTATCGTTTAGAAAAAGTGGTCCGTTATTATGAAA
GCGGGGAGTTTAGCCCTAAAACCTTGCTTTATGGCGTGGTGCCAGCGCACCCTACGCTTTTTGTCAAAAAAGCCATTCAT
GAACGCTATGGGCTTTACAAAACCGATTATAAGATTTCAGCGGATTTTGAGATGATCATCCGCTTGTTTGTGGTGCAAAA
AATAAGCTTTTCTTATTTGAAAGAAGTGTTAGTGGTGATGCGCACCGGTGGGGTTAGCACGAGCGGGTTTAAAAGCCTTT
TATTAAGGAATAAAGAAAACATAAGAGCATGCCAAGAAAACGGCATTCAAGCGAATGTTTTCTCCATGCTTTTAAAATAC
CCTAGGAAAATCATGGGCTTATTTAAAAGGGGAAAAGGGGGGCTAAAACGAAACGATTAA

Upstream 100 bases:

>100_bases
AAACATCGCTACGCATGTGAATGGAACGACCATGAATTTAGACAAACAAGTGAATTTGTTTAAAACTTAATTAAAGGGAG
TTTATCACAAAAGGGTTGGA

Downstream 100 bases:

>100_bases
AGAAAGATTAGCGTTAAATCGTGGTTTGAATCTTTCTTGATGGTTGTTGTAGGTGTAAAAAACATTCCTCACTAAAGGCA
TTTCAATCCCTAATTGCAAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLKVSVITACFNSEKTIEDTILSVLNQTYKNIEYIIIDGASADSTLEIIQKYRDRIACVVSEKDEGIYDAMNKGIKRSSG
DIIALLNSDDFYKDEFVVEKVVHEFENKNCDSVYADLVFVKPYRLEKVVRYYESGEFSPKTLLYGVVPAHPTLFVKKAIH
ERYGLYKTDYKISADFEMIIRLFVVQKISFSYLKEVLVVMRTGGVSTSGFKSLLLRNKENIRACQENGIQANVFSMLLKY
PRKIMGLFKRGKGGLKRND

Sequences:

>Translated_259_residues
MLKVSVITACFNSEKTIEDTILSVLNQTYKNIEYIIIDGASADSTLEIIQKYRDRIACVVSEKDEGIYDAMNKGIKRSSG
DIIALLNSDDFYKDEFVVEKVVHEFENKNCDSVYADLVFVKPYRLEKVVRYYESGEFSPKTLLYGVVPAHPTLFVKKAIH
ERYGLYKTDYKISADFEMIIRLFVVQKISFSYLKEVLVVMRTGGVSTSGFKSLLLRNKENIRACQENGIQANVFSMLLKY
PRKIMGLFKRGKGGLKRND
>Mature_259_residues
MLKVSVITACFNSEKTIEDTILSVLNQTYKNIEYIIIDGASADSTLEIIQKYRDRIACVVSEKDEGIYDAMNKGIKRSSG
DIIALLNSDDFYKDEFVVEKVVHEFENKNCDSVYADLVFVKPYRLEKVVRYYESGEFSPKTLLYGVVPAHPTLFVKKAIH
ERYGLYKTDYKISADFEMIIRLFVVQKISFSYLKEVLVVMRTGGVSTSGFKSLLLRNKENIRACQENGIQANVFSMLLKY
PRKIMGLFKRGKGGLKRND

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788368, Length=212, Percent_Identity=28.3018867924528, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 29632; Mature: 29632

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVSVITACFNSEKTIEDTILSVLNQTYKNIEYIIIDGASADSTLEIIQKYRDRIACVV
CEEEEEEHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHEEEE
SEKDEGIYDAMNKGIKRSSGDIIALLNSDDFYKDEFVVEKVVHEFENKNCDSVYADLVFV
CCCCCCHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHEE
KPYRLEKVVRYYESGEFSPKTLLYGVVPAHPTLFVKKAIHERYGLYKTDYKISADFEMII
CHHHHHHHHHHHHCCCCCCHHEEEECCCCCHHHHHHHHHHHHHCCEECCEEECCCHHHHH
RLFVVQKISFSYLKEVLVVMRTGGVSTSGFKSLLLRNKENIRACQENGIQANVFSMLLKY
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHH
PRKIMGLFKRGKGGLKRND
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLKVSVITACFNSEKTIEDTILSVLNQTYKNIEYIIIDGASADSTLEIIQKYRDRIACVV
CEEEEEEHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHEEEE
SEKDEGIYDAMNKGIKRSSGDIIALLNSDDFYKDEFVVEKVVHEFENKNCDSVYADLVFV
CCCCCCHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHEE
KPYRLEKVVRYYESGEFSPKTLLYGVVPAHPTLFVKKAIHERYGLYKTDYKISADFEMII
CHHHHHHHHHHHHCCCCCCHHEEEECCCCCHHHHHHHHHHHHHCCEECCEEECCCHHHHH
RLFVVQKISFSYLKEVLVVMRTGGVSTSGFKSLLLRNKENIRACQENGIQANVFSMLLKY
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHH
PRKIMGLFKRGKGGLKRND
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]