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The map label for this gene is serA [C]

Identifier: 108562521

GI number: 108562521

Start: 98188

End: 99132

Strand: Direct

Name: serA [C]

Synonym: HPAG1_0096

Alternate gene names: 108562521

Gene position: 98188-99132 (Clockwise)

Preceding gene: 108562518

Following gene: 108562524

Centisome position: 6.15

GC content: 40.0

Gene sequence:

>945_bases
ATGAAAACATTCAAAAAAGGCGTGGTATTAGACGCTAAAAGCGTGGGGTTAAAAGCGCTAGAAGTTTTAAAAGAAGTAGC
GGATTTTGATTTTTATGAGGTTACTTCGCCTAATCAAATCGTTGAACGATCAATTGAAGCTGAAATTATGGTATTGAATA
AAGTCGTGATTACTAAAGAGGTTTTAAGCCAATTACCTAAACTCAAACTCATTTGCATCACCGCTACAGGCACGGATAAT
GTGGATATAAAAAGTGCGAAAGATTTAGGCATAGAAGTCAAAAACGTGAGCGCTTATTCTACGGAATCTGTAGCCCAGCA
CACTTTAGCGTGCGCGTTGTCTTTGTTGGGGAGGATCAATGACTACGATCGTTATTGCAAAAGTGGGGAATACAGCCAAA
GCGATCTTTTTACGCACATTAGCGGTATGAAAATGGGGCTTATTAAAGGGGGTCAATGGGGGGTTATTGGTTTAGGCGCA
ATCGGTAAAAGAGTCGCCAAACTCGCCCAAGCTTTTGGGGCAAAGGTGGTGTATTATTCCCCTAAAGATAAAAAAGAAGA
GTATGAGCGCTTGAGTTTAGAGGAATTGCTTAAAACAAGCGGTATTATCAGCATTCATGCCCCCTTAAATGAAAGCACGC
GCGATTTAATCGCTCTGAAAGAATTGCAAAGCCTAAAAGAAGGGGCGATTTTAATCAATGTGGGGCGTGGGGGCATTGTG
AATGAAAAGGATCTGGCTTTAACTTTAGAAACCAAAGATTTGTATTACGCGAGCGATGTGTTTGTGAAAGAGCCTTTTGA
AAAAGATCATGCGTTTTTGAACCCAAAGATCCAAAACAAATTGCTTTTAACCCCCCATATCGCATGGGCGTATAGCGACA
GCTTGAAAACCTTAGTAGAAAAAACCAAAGAAAATATCCAGGATTTTTTAGCTTCTCAAAAATAA

Upstream 100 bases:

>100_bases
GGAGGGTGTTTTTCTTTGGTTTTGGCATGTTTAGTTTCTTTTAAAAAAGGATTTTGTGGTTTAATTATATAATTTTAAAA
CTAATTCTAGGAGTATAGAG

Downstream 100 bases:

>100_bases
ACGCAAAAAAAAGGGGGGGTTTAATCGTTTGGTTTTTTCCTTTTTGTTTGCCATAAAATAAAGTCTAAAAAATGGAGTAA
GGTTTAACCCATTAAGAAAA

Product: 2-hydroxyacid dehydrogenase

Products: 3-phosphohydroxypyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKTFKKGVVLDAKSVGLKALEVLKEVADFDFYEVTSPNQIVERSIEAEIMVLNKVVITKEVLSQLPKLKLICITATGTDN
VDIKSAKDLGIEVKNVSAYSTESVAQHTLACALSLLGRINDYDRYCKSGEYSQSDLFTHISGMKMGLIKGGQWGVIGLGA
IGKRVAKLAQAFGAKVVYYSPKDKKEEYERLSLEELLKTSGIISIHAPLNESTRDLIALKELQSLKEGAILINVGRGGIV
NEKDLALTLETKDLYYASDVFVKEPFEKDHAFLNPKIQNKLLLTPHIAWAYSDSLKTLVEKTKENIQDFLASQK

Sequences:

>Translated_314_residues
MKTFKKGVVLDAKSVGLKALEVLKEVADFDFYEVTSPNQIVERSIEAEIMVLNKVVITKEVLSQLPKLKLICITATGTDN
VDIKSAKDLGIEVKNVSAYSTESVAQHTLACALSLLGRINDYDRYCKSGEYSQSDLFTHISGMKMGLIKGGQWGVIGLGA
IGKRVAKLAQAFGAKVVYYSPKDKKEEYERLSLEELLKTSGIISIHAPLNESTRDLIALKELQSLKEGAILINVGRGGIV
NEKDLALTLETKDLYYASDVFVKEPFEKDHAFLNPKIQNKLLLTPHIAWAYSDSLKTLVEKTKENIQDFLASQK
>Mature_314_residues
MKTFKKGVVLDAKSVGLKALEVLKEVADFDFYEVTSPNQIVERSIEAEIMVLNKVVITKEVLSQLPKLKLICITATGTDN
VDIKSAKDLGIEVKNVSAYSTESVAQHTLACALSLLGRINDYDRYCKSGEYSQSDLFTHISGMKMGLIKGGQWGVIGLGA
IGKRVAKLAQAFGAKVVYYSPKDKKEEYERLSLEELLKTSGIISIHAPLNESTRDLIALKELQSLKEGAILINVGRGGIV
NEKDLALTLETKDLYYASDVFVKEPFEKDHAFLNPKIQNKLLLTPHIAWAYSDSLKTLVEKTKENIQDFLASQK

Specific function: Serine biosynthesis; first step. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=307, Percent_Identity=34.2019543973941, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI4557497, Length=294, Percent_Identity=34.6938775510204, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI145580578, Length=294, Percent_Identity=32.6530612244898, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI4557499, Length=294, Percent_Identity=32.6530612244898, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI145580575, Length=294, Percent_Identity=32.6530612244898, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI23308577, Length=247, Percent_Identity=29.1497975708502, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI6912396, Length=239, Percent_Identity=27.6150627615063, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1789279, Length=290, Percent_Identity=29.3103448275862, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87082289, Length=305, Percent_Identity=27.5409836065574, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1787645, Length=274, Percent_Identity=25.5474452554745, Blast_Score=98, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI25147481, Length=307, Percent_Identity=28.9902280130293, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17532191, Length=273, Percent_Identity=32.967032967033, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=304, Percent_Identity=25.9868421052632, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6320925, Length=306, Percent_Identity=25.1633986928105, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322116, Length=306, Percent_Identity=24.5098039215686, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324055, Length=257, Percent_Identity=25.6809338521401, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24646446, Length=298, Percent_Identity=32.2147651006711, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24646448, Length=298, Percent_Identity=32.2147651006711, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24646452, Length=298, Percent_Identity=32.2147651006711, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24646450, Length=298, Percent_Identity=32.2147651006711, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI62472511, Length=298, Percent_Identity=32.5503355704698, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI28574286, Length=303, Percent_Identity=27.0627062706271, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI19921140, Length=288, Percent_Identity=26.7361111111111, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28571528, Length=267, Percent_Identity=29.2134831460674, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585516, Length=245, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24585514, Length=256, Percent_Identity=23.828125, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI28574282, Length=256, Percent_Identity=23.828125, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI45552429, Length=256, Percent_Identity=23.828125, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI28574284, Length=256, Percent_Identity=23.828125, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45551003, Length=256, Percent_Identity=23.828125, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.95

Molecular weight: Translated: 34744; Mature: 34744

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFKKGVVLDAKSVGLKALEVLKEVADFDFYEVTSPNQIVERSIEAEIMVLNKVVITKE
CCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHEEEHHHHHHHHH
VLSQLPKLKLICITATGTDNVDIKSAKDLGIEVKNVSAYSTESVAQHTLACALSLLGRIN
HHHHCCCEEEEEEEECCCCCCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
DYDRYCKSGEYSQSDLFTHISGMKMGLIKGGQWGVIGLGAIGKRVAKLAQAFGAKVVYYS
HHHHHHCCCCCCHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCEEEEEC
PKDKKEEYERLSLEELLKTSGIISIHAPLNESTRDLIALKELQSLKEGAILINVGRGGIV
CCCHHHHHHHCCHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCC
NEKDLALTLETKDLYYASDVFVKEPFEKDHAFLNPKIQNKLLLTPHIAWAYSDSLKTLVE
CCCCEEEEEECCCEEEECCHHEECCCCCCCCEECCCCCCEEEECCCCHHHHHHHHHHHHH
KTKENIQDFLASQK
HHHHHHHHHHHCCC
>Mature Secondary Structure
MKTFKKGVVLDAKSVGLKALEVLKEVADFDFYEVTSPNQIVERSIEAEIMVLNKVVITKE
CCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHEEEHHHHHHHHH
VLSQLPKLKLICITATGTDNVDIKSAKDLGIEVKNVSAYSTESVAQHTLACALSLLGRIN
HHHHCCCEEEEEEEECCCCCCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
DYDRYCKSGEYSQSDLFTHISGMKMGLIKGGQWGVIGLGAIGKRVAKLAQAFGAKVVYYS
HHHHHHCCCCCCHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCEEEEEC
PKDKKEEYERLSLEELLKTSGIISIHAPLNESTRDLIALKELQSLKEGAILINVGRGGIV
CCCHHHHHHHCCHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCC
NEKDLALTLETKDLYYASDVFVKEPFEKDHAFLNPKIQNKLLLTPHIAWAYSDSLKTLVE
CCCCEEEEEECCCEEEECCHHEECCCCCCCCEECCCCCCEEEECCCCHHHHHHHHHHHHH
KTKENIQDFLASQK
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-phosphoglycerate; NAD+

Specific reaction: 3-phosphoglycerate + NAD+ = 3-phosphohydroxypyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]