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The map label for this gene is mboIBM [H]

Identifier: 108562518

GI number: 108562518

Start: 95740

End: 96561

Strand: Direct

Name: mboIBM [H]

Synonym: HPAG1_0093

Alternate gene names: 108562518

Gene position: 95740-96561 (Clockwise)

Preceding gene: 108562517

Following gene: 108562521

Centisome position: 6.0

GC content: 39.66

Gene sequence:

>822_bases
ATGGGAGCGTCTTATCTTTCTTGTCCCATTTTTAAAAGCCAAGACCAGCTTTTCACCCTTTATCAAGGGGATTGCAATGA
GGTTTTGCCCCAATTTGAAAATGCTTTTGATTTGATCTTTGCTGATCCGCCTTATTTCCTTTCTAATGACGGCTTAAGCA
TACAGAGCGGTAAAATCGTGAGCGTCAATAAAGGCGATTGGGATAAAGAAAATGGGATTAACGATATTGATGAGTTTAAT
TACCAGTGGATCAACAACGCCAAAAAGGCTTTAAAAAACACAGGAAGCCTTTTAATCAGCGGGACTTACCACAATATCTT
TTCTTTGGGGCGCCTTTTACAAAAATTGGATTTTAAGATTTTAAACCTCATCACTTGGCAAAAAACCAATCCTCCTCCCA
ATTTCAGCTGCCGTTATTTGACGCATTCGGCTGAGCAAATCATTTGGGCGAGGAAAAGCCGCAAGCATAAGCATGTTTTT
AACTATGAGGTTTTAAAAAAGATCAATAATGATAAACAAATGCACGATGTGTGGAGCTTCCCAGCGATCGCTCCTTGGGA
AAAAGTTAATGGCAAGCACCCCACTCAAAAACCTCTCGCTTTATTAGTGCGCTTGCTTTTAATGGCGAGCGATGAAAATT
CCCTCATTGGCGATCCTTTTAGCGGGAGCTCTACCACAGGCATTGCGGCTAATCTTTTGAATAGGGAATTTATCGGCATA
GAAAAAGAAAGCGAGTTTATCAAAATATCCATGGACAGAAAAATAGAATTAGACGCTCGCTATAAAGAAATCCGATCTAA
AATCAAAGATTTAAACCACTAG

Upstream 100 bases:

>100_bases
GCTTGATACGCCAATCGAGATGGAGGGTTTTGATAAAGTGCTTAATGATGAAGCAATGCAAAAACACATTAAAAATAAAG
GCATTAACGCAGAATAAAAA

Downstream 100 bases:

>100_bases
TAAAGCCCCTTTTTAAGCCGCTTTAAGCGTTATACTTTTGGGATTTTACCTCAAAATGGGATTCTATTTTCACCCATTCC
TCACAAAGGATATTCTCATG

Product: type II adenine methyltransferase

Products: NA

Alternate protein names: M.MboIB; Adenine-specific methyltransferase MboIB; M.MboI C [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MGASYLSCPIFKSQDQLFTLYQGDCNEVLPQFENAFDLIFADPPYFLSNDGLSIQSGKIVSVNKGDWDKENGINDIDEFN
YQWINNAKKALKNTGSLLISGTYHNIFSLGRLLQKLDFKILNLITWQKTNPPPNFSCRYLTHSAEQIIWARKSRKHKHVF
NYEVLKKINNDKQMHDVWSFPAIAPWEKVNGKHPTQKPLALLVRLLLMASDENSLIGDPFSGSSTTGIAANLLNREFIGI
EKESEFIKISMDRKIELDARYKEIRSKIKDLNH

Sequences:

>Translated_273_residues
MGASYLSCPIFKSQDQLFTLYQGDCNEVLPQFENAFDLIFADPPYFLSNDGLSIQSGKIVSVNKGDWDKENGINDIDEFN
YQWINNAKKALKNTGSLLISGTYHNIFSLGRLLQKLDFKILNLITWQKTNPPPNFSCRYLTHSAEQIIWARKSRKHKHVF
NYEVLKKINNDKQMHDVWSFPAIAPWEKVNGKHPTQKPLALLVRLLLMASDENSLIGDPFSGSSTTGIAANLLNREFIGI
EKESEFIKISMDRKIELDARYKEIRSKIKDLNH
>Mature_272_residues
GASYLSCPIFKSQDQLFTLYQGDCNEVLPQFENAFDLIFADPPYFLSNDGLSIQSGKIVSVNKGDWDKENGINDIDEFNY
QWINNAKKALKNTGSLLISGTYHNIFSLGRLLQKLDFKILNLITWQKTNPPPNFSCRYLTHSAEQIIWARKSRKHKHVFN
YEVLKKINNDKQMHDVWSFPAIAPWEKVNGKHPTQKPLALLVRLLLMASDENSLIGDPFSGSSTTGIAANLLNREFIGIE
KESEFIKISMDRKIELDARYKEIRSKIKDLNH

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MboI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=276, Percent_Identity=27.8985507246377, Blast_Score=88, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31312; Mature: 31181

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGASYLSCPIFKSQDQLFTLYQGDCNEVLPQFENAFDLIFADPPYFLSNDGLSIQSGKIV
CCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCEEEEECCCCCEECCCCCEEECCEEE
SVNKGDWDKENGINDIDEFNYQWINNAKKALKNTGSLLISGTYHNIFSLGRLLQKLDFKI
EECCCCCCCCCCCCCHHHHCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHH
LNLITWQKTNPPPNFSCRYLTHSAEQIIWARKSRKHKHVFNYEVLKKINNDKQMHDVWSF
HHHEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHCC
PAIAPWEKVNGKHPTQKPLALLVRLLLMASDENSLIGDPFSGSSTTGIAANLLNREFIGI
CCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
EKESEFIKISMDRKIELDARYKEIRSKIKDLNH
CCCCCEEEEECCCEEECHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GASYLSCPIFKSQDQLFTLYQGDCNEVLPQFENAFDLIFADPPYFLSNDGLSIQSGKIV
CCCCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCEEEEECCCCCEECCCCCEEECCEEE
SVNKGDWDKENGINDIDEFNYQWINNAKKALKNTGSLLISGTYHNIFSLGRLLQKLDFKI
EECCCCCCCCCCCCCHHHHCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHH
LNLITWQKTNPPPNFSCRYLTHSAEQIIWARKSRKHKHVFNYEVLKKINNDKQMHDVWSF
HHHEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHCC
PAIAPWEKVNGKHPTQKPLALLVRLLLMASDENSLIGDPFSGSSTTGIAANLLNREFIGI
CCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
EKESEFIKISMDRKIELDARYKEIRSKIKDLNH
CCCCCEEEEECCCEEECHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8506128 [H]