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The map label for this gene is nolK [H]

Identifier: 108562467

GI number: 108562467

Start: 44295

End: 45227

Strand: Direct

Name: nolK [H]

Synonym: HPAG1_0042

Alternate gene names: 108562467

Gene position: 44295-45227 (Clockwise)

Preceding gene: 108562466

Following gene: 108562479

Centisome position: 2.77

GC content: 40.94

Gene sequence:

>933_bases
ATGAATGAGATTATTTTAATCACCGGTGCCTATGGCATGGTGGGGCAGAACACGGCGTTGTATTTTAAGAAAAATAAGCC
TGATGTTACTTTACTCACCCCTAAAAAGAGCGAATTGTATTTGTTGGATAAGGACAACATTCAAGCTTATTTGAAAGAAT
ACAAACCTACAGGCATTATCCATTGCGCTGGGAGAGTGGGGGGCATTGTCGCTAACATGAACGATCTTTCAACTTACATG
GTTGAGAATTTACTCATGGGTTTGTATCTTTTTTCTAGCGCTTTAGATTCGGGCGTGAAAAAAGCCATTAATCTAGCGAG
CTCTTGCGCTTATCCTAAATTCGCCCCTAACCCTTTAAAAGAGAGCGATTTATTGAACGGCTCTTTAGAGCCAACGAACG
AAGGCTACGCTTTAGCCAAACTCTCTGTGATGAAGTATTGCGAGTATGTGAGCGCTGAAAAGGGCGTTTTTTATAAAACT
CTAGTGCCTTGCAACCTTTATGGCGAGTTTGACAAGTTTGAAGAAAAAATAGCGCACATGATACCAGGGCTTATTGCTAG
GATGCACGCCGCTAAATTAAAAAATGAAAAAGAGTTTGTGATGTGGGGCGATGGCACGGCCAGGAGAGAATATCTAAACG
CTAAAGATTTAGCCAGGTTCATCGCCCTAGCTTATGACAATATCACTTCAATCCCTAGCGTGATGAATGTCGGCTCTGGA
GTGGATTACAGCATTGAAGAGTATTATGAAATGGTCGCTCAGGTTTTAGACTATAAGGGCGTGTTTGTCAAAGATTTATC
CAAACCAGTGGGCATGCAGCAAAAGCTTATGGATATTTCCAAACAAAAGGCTTTAAAATGGGAATTAGAAATCCCTTTAG
AGCAGGGCATCAAAGAAGCTTATGAGTATTATTTGAAGCTTTTAGAGGTTTGA

Upstream 100 bases:

>100_bases
GATTTAAAAGAGTTGGTTAAGGACATGTTAGAATACGATTTAAAAGAATGCCAGAAAAACCTTTACTTGCAAGATGGGGG
CTATACTTTAAGGAATTTTT

Downstream 100 bases:

>100_bases
AATAAAATCAAGGCTCTTATAGGGTTATAAAAACGCTCCGCCATTAAGCGTCAGGCTAGCGGTAGTTGCGATATTGTGAT
CGCTTTAGCTTCAAACAAAC

Product: GDP-fucose synthetase

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MNEIILITGAYGMVGQNTALYFKKNKPDVTLLTPKKSELYLLDKDNIQAYLKEYKPTGIIHCAGRVGGIVANMNDLSTYM
VENLLMGLYLFSSALDSGVKKAINLASSCAYPKFAPNPLKESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVFYKT
LVPCNLYGEFDKFEEKIAHMIPGLIARMHAAKLKNEKEFVMWGDGTARREYLNAKDLARFIALAYDNITSIPSVMNVGSG
VDYSIEEYYEMVAQVLDYKGVFVKDLSKPVGMQQKLMDISKQKALKWELEIPLEQGIKEAYEYYLKLLEV

Sequences:

>Translated_310_residues
MNEIILITGAYGMVGQNTALYFKKNKPDVTLLTPKKSELYLLDKDNIQAYLKEYKPTGIIHCAGRVGGIVANMNDLSTYM
VENLLMGLYLFSSALDSGVKKAINLASSCAYPKFAPNPLKESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVFYKT
LVPCNLYGEFDKFEEKIAHMIPGLIARMHAAKLKNEKEFVMWGDGTARREYLNAKDLARFIALAYDNITSIPSVMNVGSG
VDYSIEEYYEMVAQVLDYKGVFVKDLSKPVGMQQKLMDISKQKALKWELEIPLEQGIKEAYEYYLKLLEV
>Mature_310_residues
MNEIILITGAYGMVGQNTALYFKKNKPDVTLLTPKKSELYLLDKDNIQAYLKEYKPTGIIHCAGRVGGIVANMNDLSTYM
VENLLMGLYLFSSALDSGVKKAINLASSCAYPKFAPNPLKESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVFYKT
LVPCNLYGEFDKFEEKIAHMIPGLIARMHAAKLKNEKEFVMWGDGTARREYLNAKDLARFIALAYDNITSIPSVMNVGSG
VDYSIEEYYEMVAQVLDYKGVFVKDLSKPVGMQQKLMDISKQKALKWELEIPLEQGIKEAYEYYLKLLEV

Specific function: Probable nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=306, Percent_Identity=27.1241830065359, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI1788365, Length=310, Percent_Identity=37.0967741935484, Blast_Score=218, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17554428, Length=309, Percent_Identity=25.8899676375405, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI19922778, Length=311, Percent_Identity=27.6527331189711, Blast_Score=142, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 34860; Mature: 34860

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEIILITGAYGMVGQNTALYFKKNKPDVTLLTPKKSELYLLDKDNIQAYLKEYKPTGII
CCCEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCCEEEEECCHHHHHHHHCCCCCEE
HCAGRVGGIVANMNDLSTYMVENLLMGLYLFSSALDSGVKKAINLASSCAYPKFAPNPLK
EECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVFYKTLVPCNLYGEFDKFEEKIAHM
CHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
IPGLIARMHAAKLKNEKEFVMWGDGTARREYLNAKDLARFIALAYDNITSIPSVMNVGSG
HHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHCCCC
VDYSIEEYYEMVAQVLDYKGVFVKDLSKPVGMQQKLMDISKQKALKWELEIPLEQGIKEA
CCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHCEEEEEECCHHHHHHHH
YEYYLKLLEV
HHHHHHHHCC
>Mature Secondary Structure
MNEIILITGAYGMVGQNTALYFKKNKPDVTLLTPKKSELYLLDKDNIQAYLKEYKPTGII
CCCEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCCEEEEECCHHHHHHHHCCCCCEE
HCAGRVGGIVANMNDLSTYMVENLLMGLYLFSSALDSGVKKAINLASSCAYPKFAPNPLK
EECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVFYKTLVPCNLYGEFDKFEEKIAHM
CHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
IPGLIARMHAAKLKNEKEFVMWGDGTARREYLNAKDLARFIALAYDNITSIPSVMNVGSG
HHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHCCCC
VDYSIEEYYEMVAQVLDYKGVFVKDLSKPVGMQQKLMDISKQKALKWELEIPLEQGIKEA
CCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHCEEEEEECCHHHHHHHH
YEYYLKLLEV
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1472718 [H]