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The map label for this gene is eptA [H]

Identifier: 108562447

GI number: 108562447

Start: 22422

End: 23993

Strand: Reverse

Name: eptA [H]

Synonym: HPAG1_0022

Alternate gene names: 108562447

Gene position: 23993-22422 (Counterclockwise)

Preceding gene: 108562448

Following gene: 108562446

Centisome position: 1.5

GC content: 39.82

Gene sequence:

>1572_bases
TTGGCATCATTACTCTATCTGAAGTTTTTAAAACCCCTGAGTTGTCTGCAAGCCGGTTTGCTTTATAGCCTTATTTTTGG
CGTTTTATACCATTTCCCTTTGTTCGCTTATGTTTATAAAGAAAGCAACCAGGTTAGTTTTATCGCCATGATGGTTGTGG
TGCTTTTTTGCGTTAATGGCGCTCTTTTTTTGGGATTAGGCTTGATCTCTGCTTCTTTGATGCGTTGGAGTGCAATAGTT
TTTAGTTGGCTCAATTCTGTCGCTTTCTATTTCATTAGCGCTTATAAGGTGTTTTTAAATAAAAGCATGATGGGCAATGT
CTTAAACACCAACACGCATGAAGTTTTAGGCTTTTTGAGCGTTAAATTATTCGTTTTTATCGTTGTTTTTGGGGTGTTAC
CCGGCTATGTCATCTATAAAATCCCCCTTAAAAATTCTTCTAAAAAAGCGCCCTTTTTAGCGATCTTGGCGTTAGTGTTT
ATTTTTATCGCTAGCGCTTTTATGAACGCTAAAAATTGGCTGTGGTTTGACAAGCATGCGAAATTCATAGGGGGCTTAAT
TTTGCCCTTCGCTTATAGCGTGAACGCCTTTAGAGTGAGCGCTCTTAAATTTTTCGCCCCCACTATCAAGCCGCTCCCTC
TCTTTTCACCCAATCATTCTCATTCGTTTGTGGTGCTAGTCATTGGCGAAAGCGCTAGGAAGCATAATTACGCCCTTTAT
GGCTATCACAAACCCACCACCCCAAGATTAAGCAAACGCTTAAAAAATCACGAACTCACTCTTTTTAACGCCACTTCTTG
CGCCACTTACACGACAGCGAGTTTGGAATGCATTTTAGATTCTTCTTTTAAAAACAGCTTGGGCGTTTATGAAAACTTGC
CCACTTACTTGACTAAAGCCGGTATCAAAGTCTTTTGGTATAGCGCGAATGACGGCGAAAAGAACGTTAAGGTTACCAGC
TATCTTAAAAACTATGAATTGATTCAAAAATGCCCCAATTGCGAGGCGATCGCCCCTTATGATGAATCCTTACTCTATAA
TTTACCTGACCTTTTAAAAGAACACTCTAACGAAAATGTCTTACTCATCTTACACCTTGCAGGCTCGCATGGCCCAAACT
ACGACAACAAAGTGCCTTTAAATTTTAGGGTGTTTAAGCCCTATTGCTCAAGCGCTAATCTGTCTTCTTGCTCCAAAGAA
AGCCTGATTAACGCCTATGACAACACCATTTTTTATAACGACTATCTGTTAGACAAAATCATTAGCATGCTCAAAAAAGC
CAAACAGCCCGCTTTAATGATTTATTTAAGCGATCATGGCGAAAGTTTGGGCGAAGAAGCGTTCTATTTGCATGGCATTC
CTAAAAGCATCGCCCCCAAAGAACAATACGAGATCCCCTTTATCGTTTATGCTAATGAGCCTTTCAAAGAGAAGCATTCC
ATCATTCAAACCCAAACCCCCATTAATCAAAACGTTATTTTCCATAGCATTTTAGGGGTGTTTTTGGATTTTAAAAACCC
TAGCGCTGTTTATCGCCCTTCTTTAGACCTGCTCAAACACAAAAAAGAGTAA

Upstream 100 bases:

>100_bases
CAAACCACCCTAAAAAACGCACTTTCTAGTCTTAAAATAAACCATAAAATCCCCATACCTACGATATAATCCTAAGATTG
ATTGATTTTAAGGGGTGTGT

Downstream 100 bases:

>100_bases
ATAACACGCATGAAAAGATTTAAACAAAAATTTTGTGAAAGCCTGCCCAAAAGCTTTTCTAAAACTTTGTTAGCATTCAG
TTTGGGCTTGATTTTATTGG

Product: lipid A phosphoethanolamine transferase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MASLLYLKFLKPLSCLQAGLLYSLIFGVLYHFPLFAYVYKESNQVSFIAMMVVVLFCVNGALFLGLGLISASLMRWSAIV
FSWLNSVAFYFISAYKVFLNKSMMGNVLNTNTHEVLGFLSVKLFVFIVVFGVLPGYVIYKIPLKNSSKKAPFLAILALVF
IFIASAFMNAKNWLWFDKHAKFIGGLILPFAYSVNAFRVSALKFFAPTIKPLPLFSPNHSHSFVVLVIGESARKHNYALY
GYHKPTTPRLSKRLKNHELTLFNATSCATYTTASLECILDSSFKNSLGVYENLPTYLTKAGIKVFWYSANDGEKNVKVTS
YLKNYELIQKCPNCEAIAPYDESLLYNLPDLLKEHSNENVLLILHLAGSHGPNYDNKVPLNFRVFKPYCSSANLSSCSKE
SLINAYDNTIFYNDYLLDKIISMLKKAKQPALMIYLSDHGESLGEEAFYLHGIPKSIAPKEQYEIPFIVYANEPFKEKHS
IIQTQTPINQNVIFHSILGVFLDFKNPSAVYRPSLDLLKHKKE

Sequences:

>Translated_523_residues
MASLLYLKFLKPLSCLQAGLLYSLIFGVLYHFPLFAYVYKESNQVSFIAMMVVVLFCVNGALFLGLGLISASLMRWSAIV
FSWLNSVAFYFISAYKVFLNKSMMGNVLNTNTHEVLGFLSVKLFVFIVVFGVLPGYVIYKIPLKNSSKKAPFLAILALVF
IFIASAFMNAKNWLWFDKHAKFIGGLILPFAYSVNAFRVSALKFFAPTIKPLPLFSPNHSHSFVVLVIGESARKHNYALY
GYHKPTTPRLSKRLKNHELTLFNATSCATYTTASLECILDSSFKNSLGVYENLPTYLTKAGIKVFWYSANDGEKNVKVTS
YLKNYELIQKCPNCEAIAPYDESLLYNLPDLLKEHSNENVLLILHLAGSHGPNYDNKVPLNFRVFKPYCSSANLSSCSKE
SLINAYDNTIFYNDYLLDKIISMLKKAKQPALMIYLSDHGESLGEEAFYLHGIPKSIAPKEQYEIPFIVYANEPFKEKHS
IIQTQTPINQNVIFHSILGVFLDFKNPSAVYRPSLDLLKHKKE
>Mature_522_residues
ASLLYLKFLKPLSCLQAGLLYSLIFGVLYHFPLFAYVYKESNQVSFIAMMVVVLFCVNGALFLGLGLISASLMRWSAIVF
SWLNSVAFYFISAYKVFLNKSMMGNVLNTNTHEVLGFLSVKLFVFIVVFGVLPGYVIYKIPLKNSSKKAPFLAILALVFI
FIASAFMNAKNWLWFDKHAKFIGGLILPFAYSVNAFRVSALKFFAPTIKPLPLFSPNHSHSFVVLVIGESARKHNYALYG
YHKPTTPRLSKRLKNHELTLFNATSCATYTTASLECILDSSFKNSLGVYENLPTYLTKAGIKVFWYSANDGEKNVKVTSY
LKNYELIQKCPNCEAIAPYDESLLYNLPDLLKEHSNENVLLILHLAGSHGPNYDNKVPLNFRVFKPYCSSANLSSCSKES
LINAYDNTIFYNDYLLDKIISMLKKAKQPALMIYLSDHGESLGEEAFYLHGIPKSIAPKEQYEIPFIVYANEPFKEKHSI
IQTQTPINQNVIFHSILGVFLDFKNPSAVYRPSLDLLKHKKE

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=505, Percent_Identity=29.1089108910891, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI87082286, Length=278, Percent_Identity=30.5755395683453, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87082223, Length=408, Percent_Identity=26.2254901960784, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790392, Length=374, Percent_Identity=25.9358288770053, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787035, Length=277, Percent_Identity=25.2707581227437, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 59176; Mature: 59045

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLLYLKFLKPLSCLQAGLLYSLIFGVLYHFPLFAYVYKESNQVSFIAMMVVVLFCVNG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
ALFLGLGLISASLMRWSAIVFSWLNSVAFYFISAYKVFLNKSMMGNVLNTNTHEVLGFLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VKLFVFIVVFGVLPGYVIYKIPLKNSSKKAPFLAILALVFIFIASAFMNAKNWLWFDKHA
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHH
KFIGGLILPFAYSVNAFRVSALKFFAPTIKPLPLFSPNHSHSFVVLVIGESARKHNYALY
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEE
GYHKPTTPRLSKRLKNHELTLFNATSCATYTTASLECILDSSFKNSLGVYENLPTYLTKA
EECCCCCHHHHHHHCCCCEEEEECCCCCEEEHHHHHEEECCCHHHCCCCHHCCHHHHHHC
GIKVFWYSANDGEKNVKVTSYLKNYELIQKCPNCEAIAPYDESLLYNLPDLLKEHSNENV
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHCCHHHHHHCCCCCE
LLILHLAGSHGPNYDNKVPLNFRVFKPYCSSANLSSCSKESLINAYDNTIFYNDYLLDKI
EEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCEEEEHHHHHHHH
ISMLKKAKQPALMIYLSDHGESLGEEAFYLHGIPKSIAPKEQYEIPFIVYANEPFKEKHS
HHHHHHCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHH
IIQTQTPINQNVIFHSILGVFLDFKNPSAVYRPSLDLLKHKKE
HEECCCCCCCHHHHHHHHHHHEECCCCCCEECCCHHHHHCCCC
>Mature Secondary Structure 
ASLLYLKFLKPLSCLQAGLLYSLIFGVLYHFPLFAYVYKESNQVSFIAMMVVVLFCVNG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
ALFLGLGLISASLMRWSAIVFSWLNSVAFYFISAYKVFLNKSMMGNVLNTNTHEVLGFLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VKLFVFIVVFGVLPGYVIYKIPLKNSSKKAPFLAILALVFIFIASAFMNAKNWLWFDKHA
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHH
KFIGGLILPFAYSVNAFRVSALKFFAPTIKPLPLFSPNHSHSFVVLVIGESARKHNYALY
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEE
GYHKPTTPRLSKRLKNHELTLFNATSCATYTTASLECILDSSFKNSLGVYENLPTYLTKA
EECCCCCHHHHHHHCCCCEEEEECCCCCEEEHHHHHEEECCCHHHCCCCHHCCHHHHHHC
GIKVFWYSANDGEKNVKVTSYLKNYELIQKCPNCEAIAPYDESLLYNLPDLLKEHSNENV
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHCCHHHHHHCCCCCE
LLILHLAGSHGPNYDNKVPLNFRVFKPYCSSANLSSCSKESLINAYDNTIFYNDYLLDKI
EEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCEEEEHHHHHHHH
ISMLKKAKQPALMIYLSDHGESLGEEAFYLHGIPKSIAPKEQYEIPFIVYANEPFKEKHS
HHHHHHCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHH
IIQTQTPINQNVIFHSILGVFLDFKNPSAVYRPSLDLLKHKKE
HEECCCCCCCHHHHHHHHHHHEECCCCCCEECCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]