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The map label for this gene is yeiT [H]

Identifier: 107023147

GI number: 107023147

Start: 1771888

End: 1773225

Strand: Reverse

Name: yeiT [H]

Synonym: Bcen_1596

Alternate gene names: 107023147

Gene position: 1773225-1771888 (Counterclockwise)

Preceding gene: 107023148

Following gene: 107023146

Centisome position: 53.82

GC content: 70.63

Gene sequence:

>1338_bases
ATGACCACCCAGCCAACCGGCGATATCGCCGCGCATCGCCTGTCGTCCACGCAACTCTCGTGCGAATTCGCCGATATCGC
GCCGCTGCTCGATCCGACTGCCGCAGCGGCCGCCGCAAGCCGCTGTCATTACTGCTACGACGCGCCGTGCGTGCATGCGT
GCCCGACGCAGATCGACATCCCGAGCTTCATCCGCAAGATCGGCAACGGCAATCTGAAAGGCGCGGCGACTGACATCCTG
TCCGCGAATCCGCTCGGCGGGATGTGCGCACGCGTGTGCCCGACCGAGATCCTGTGTGAAGGCGCGTGCGTGCGCAACCA
TCAGGATGCGCAGCCCGTCGCGATCGGCGCGCTGCAGCGGCACGCGACCGACTGGGCGATGCAGACCGGCGCGGTGCAGT
TCCGGCGCGCGCCCGAGACGGGCCGCCATGTGGCGGTGGTCGGCGCGGGGCCGGCCGGGCTCGCGTGCGCGCACCGGCTC
GCGCTCGCCGGGCATCGCGTCACGCTGTTCGACGCACGCCCGAAGGCCGGCGGCCTCAACGAATACGGGATCGCCGCGTA
CAAGACCGTCGACGATTTCGCGCAACGCGAAGTCGAGTGGTTGCTGTCGGTGGGCGGCATCACGCTGGAAACGGGAGTGG
CGCTTGGCCGCGACGTGACGCTCGACACGCTGCGCGAGCAGCACGACGCGGTATTCCTTGCGATGGGCCTCGGCGGCGTG
CGTGCGCTCGCGATCGACGGCGAGCAACTGCGCGGCGTGATGAACGCGGTCGATTTCATCGAGCAGGTGCGGCAGGCCGA
CACGCTCGCGAACGTGCCGGTCGGCCGGCGCGTGGTCGTGATCGGCGGCGGCAATACGGCGATCGATGCGGCGGTGCAGA
GCCGCAAGCTCGGTGCGGAGCGCGTGACGATGGTGTATCGGCGCGGCGTGGACGCGATGAGCGCGACATGGGCCGAGCGC
GAGTTCGCGCAGAAGAGCGGCGTCACGCTGGTCACGAACGCGAAGCCGGTGCGCATTGCGGGCACGGACGGGCAGGTGAC
GGGCGTCGAATTCGAAGGCGCATCGGGCGAGCGTTTCACCGTCGACGCGGACATGGTGCTGAAGGCGATCGGCCAGACGC
TGGTGCCGGACGGTGTCGAAGCCGCGCTGCTGACCGCGGACGGCACCCGTATCGCGGTGGATGCGGACGGACGCACGGCG
CTGCCGGACGTATGGGCCGGCGGCGACTGCGCGGCGACGGGCGGCATCGACCTCACGGTGCAGGCCGTGCAGGACGGCAA
GCGCGCGGCCGCGTCGATCGACGCGACGCTCGCGGCGCGCGACGCGAAGGCGGCCTGA

Upstream 100 bases:

>100_bases
GCATCACGTCAGATATTTGCATTCCTAAGCACGACCGCCCCGACTGTGCATCCGCAGCCGGCGGGGACGGCTTTGTCTCC
ACCCAGTCGAAGGAACGCGT

Downstream 100 bases:

>100_bases
CCGCGCGACGCACCGCACGCACACCGAACACGACCCCATTCTCACGGAGCCGAACATGGCCGACCTTCGCTGCACCATCG
CAGGCATCACTTCGCCGAAT

Product: oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTTQPTGDIAAHRLSSTQLSCEFADIAPLLDPTAAAAAASRCHYCYDAPCVHACPTQIDIPSFIRKIGNGNLKGAATDIL
SANPLGGMCARVCPTEILCEGACVRNHQDAQPVAIGALQRHATDWAMQTGAVQFRRAPETGRHVAVVGAGPAGLACAHRL
ALAGHRVTLFDARPKAGGLNEYGIAAYKTVDDFAQREVEWLLSVGGITLETGVALGRDVTLDTLREQHDAVFLAMGLGGV
RALAIDGEQLRGVMNAVDFIEQVRQADTLANVPVGRRVVVIGGGNTAIDAAVQSRKLGAERVTMVYRRGVDAMSATWAER
EFAQKSGVTLVTNAKPVRIAGTDGQVTGVEFEGASGERFTVDADMVLKAIGQTLVPDGVEAALLTADGTRIAVDADGRTA
LPDVWAGGDCAATGGIDLTVQAVQDGKRAAASIDATLAARDAKAA

Sequences:

>Translated_445_residues
MTTQPTGDIAAHRLSSTQLSCEFADIAPLLDPTAAAAAASRCHYCYDAPCVHACPTQIDIPSFIRKIGNGNLKGAATDIL
SANPLGGMCARVCPTEILCEGACVRNHQDAQPVAIGALQRHATDWAMQTGAVQFRRAPETGRHVAVVGAGPAGLACAHRL
ALAGHRVTLFDARPKAGGLNEYGIAAYKTVDDFAQREVEWLLSVGGITLETGVALGRDVTLDTLREQHDAVFLAMGLGGV
RALAIDGEQLRGVMNAVDFIEQVRQADTLANVPVGRRVVVIGGGNTAIDAAVQSRKLGAERVTMVYRRGVDAMSATWAER
EFAQKSGVTLVTNAKPVRIAGTDGQVTGVEFEGASGERFTVDADMVLKAIGQTLVPDGVEAALLTADGTRIAVDADGRTA
LPDVWAGGDCAATGGIDLTVQAVQDGKRAAASIDATLAARDAKAA
>Mature_444_residues
TTQPTGDIAAHRLSSTQLSCEFADIAPLLDPTAAAAAASRCHYCYDAPCVHACPTQIDIPSFIRKIGNGNLKGAATDILS
ANPLGGMCARVCPTEILCEGACVRNHQDAQPVAIGALQRHATDWAMQTGAVQFRRAPETGRHVAVVGAGPAGLACAHRLA
LAGHRVTLFDARPKAGGLNEYGIAAYKTVDDFAQREVEWLLSVGGITLETGVALGRDVTLDTLREQHDAVFLAMGLGGVR
ALAIDGEQLRGVMNAVDFIEQVRQADTLANVPVGRRVVVIGGGNTAIDAAVQSRKLGAERVTMVYRRGVDAMSATWAERE
FAQKSGVTLVTNAKPVRIAGTDGQVTGVEFEGASGERFTVDADMVLKAIGQTLVPDGVEAALLTADGTRIAVDADGRTAL
PDVWAGGDCAATGGIDLTVQAVQDGKRAAASIDATLAARDAKAA

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=464, Percent_Identity=31.6810344827586, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI237757300, Length=86, Percent_Identity=51.1627906976744, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1788468, Length=413, Percent_Identity=34.6246973365617, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789606, Length=446, Percent_Identity=33.1838565022422, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI87082180, Length=475, Percent_Identity=33.2631578947368, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1788811, Length=458, Percent_Identity=33.1877729257642, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI1789243, Length=417, Percent_Identity=29.9760191846523, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789463, Length=160, Percent_Identity=35, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71984108, Length=457, Percent_Identity=32.8227571115974, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17570289, Length=450, Percent_Identity=25.1111111111111, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17543860, Length=159, Percent_Identity=32.7044025157233, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320030, Length=454, Percent_Identity=28.1938325991189, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24640763, Length=455, Percent_Identity=35.6043956043956, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI18858217, Length=455, Percent_Identity=35.6043956043956, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI28574881, Length=450, Percent_Identity=28.6666666666667, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24665539, Length=450, Percent_Identity=28.6666666666667, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24665547, Length=450, Percent_Identity=28.6666666666667, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24665543, Length=450, Percent_Identity=28.6666666666667, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 46255; Mature: 46124

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQPTGDIAAHRLSSTQLSCEFADIAPLLDPTAAAAAASRCHYCYDAPCVHACPTQIDI
CCCCCCCHHHHHHHCCCEEEEEHHHHHHCCCCHHHHHHHHHCCEECCCCCHHCCCCCCCH
PSFIRKIGNGNLKGAATDILSANPLGGMCARVCPTEILCEGACVRNHQDAQPVAIGALQR
HHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHCCHHHHHCCCHHCCCCCCCCHHHHHHHH
HATDWAMQTGAVQFRRAPETGRHVAVVGAGPAGLACAHRLALAGHRVTLFDARPKAGGLN
HHHHHHHHCCCEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCC
EYGIAAYKTVDDFAQREVEWLLSVGGITLETGVALGRDVTLDTLREQHDAVFLAMGLGGV
CCCCEEEHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHCCEEEEEECCCCE
RALAIDGEQLRGVMNAVDFIEQVRQADTLANVPVGRRVVVIGGGNTAIDAAVQSRKLGAE
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCHH
RVTMVYRRGVDAMSATWAEREFAQKSGVTLVTNAKPVRIAGTDGQVTGVEFEGASGERFT
HHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCEEEEEEECCCCCEEE
VDADMVLKAIGQTLVPDGVEAALLTADGTRIAVDADGRTALPDVWAGGDCAATGGIDLTV
ECHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCEEECCCCEEEE
QAVQDGKRAAASIDATLAARDAKAA
EEHHCCHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
TTQPTGDIAAHRLSSTQLSCEFADIAPLLDPTAAAAAASRCHYCYDAPCVHACPTQIDI
CCCCCCHHHHHHHCCCEEEEEHHHHHHCCCCHHHHHHHHHCCEECCCCCHHCCCCCCCH
PSFIRKIGNGNLKGAATDILSANPLGGMCARVCPTEILCEGACVRNHQDAQPVAIGALQR
HHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHCCHHHHHCCCHHCCCCCCCCHHHHHHHH
HATDWAMQTGAVQFRRAPETGRHVAVVGAGPAGLACAHRLALAGHRVTLFDARPKAGGLN
HHHHHHHHCCCEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCC
EYGIAAYKTVDDFAQREVEWLLSVGGITLETGVALGRDVTLDTLREQHDAVFLAMGLGGV
CCCCEEEHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHCCEEEEEECCCCE
RALAIDGEQLRGVMNAVDFIEQVRQADTLANVPVGRRVVVIGGGNTAIDAAVQSRKLGAE
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCHH
RVTMVYRRGVDAMSATWAEREFAQKSGVTLVTNAKPVRIAGTDGQVTGVEFEGASGERFT
HHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCEEEEEEECCCCCEEE
VDADMVLKAIGQTLVPDGVEAALLTADGTRIAVDADGRTALPDVWAGGDCAATGGIDLTV
ECHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCEEECCCCEEEE
QAVQDGKRAAASIDATLAARDAKAA
EEHHCCHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]