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The map label for this gene is nfdA [H]

Identifier: 107023029

GI number: 107023029

Start: 1638669

End: 1640654

Strand: Reverse

Name: nfdA [H]

Synonym: Bcen_1477

Alternate gene names: 107023029

Gene position: 1640654-1638669 (Counterclockwise)

Preceding gene: 107023030

Following gene: 107023028

Centisome position: 49.8

GC content: 73.06

Gene sequence:

>1986_bases
ATGCCGATGCGTGCGGTGCGGCCGCTTTCCCCCGTTCGACGACCAGCAGGGCGGCCCGCGCCGCGCCGCGCGCAGGCGCG
TACCGGAGGCCCGACGGTGCTTAGAGCGACGGAACCCGCGCGTTCGGCGGACCTCGTCGTGTTCAACGGTAAGATCGCGA
CCCAGGACGAACAGCGCTCGTTCGTCCGTGCGCTCGCCGTGAAGGACGGGACCATCGTCGCGACCGGCGACGATCGCGAC
GTGCTGCGCCATGCGCACGACGACACCGCCTGTATCGACCTGCACGGCCGTACCGTGATTCCGGGCCTGATCGATGCGCA
CCTGCAGGCGATCCGCGGCGGCATGAACTTCAACCTCGAGGTGCGCTGGGACGGCGTGCCGTCGCTGGCCGACGCGCTCG
AGCTGCTGGGCCGGCAGGTACGACGCACGCCCGCGCCGCACTGGGCGCGCGTGGCGGGCGGCTGGACCGCGCTGCAGTTC
GCGGAAAAGCGCGGGCCGACGACGGCCGAGCTCAACGCGATCGCGCCCGACACGCCGGTATTCGTCCAGCATCTGGCCGA
CAGTGCGTGGCTGAATGCGGCCGCGCTGCGCGCGCTCGGCTACGGGCGAGACACGCCCGATCCGCCCGGCGGCGAACTGC
GGCGCGACCGCCATGGGCGGCCGACCGGGCTGCTGCTCGCGCGTCAGGACCCGGCGGTGCTGGACGCGGTGCTCGCCCGC
GCGCCGACGCTCGATGCGGCCGACCGGGTCAACTCGACGCGGCAGTTCATGCACGCGCTGAACCGCGTCGGCGTGACGAG
CGCGATCGACGCCGGCGGCGACGGCCTCGCGTATCCGGACGACTATGCGGCGGTCGCGACGCTCGCGCGGCGCGGCGAAT
TGACGACGCGCATCGCCTATGCGCTGGGCGCGCGGCACGCGGGCCGGGAGCTCGACGACTTCGCGAAATGGATCGCGCTG
ACCGCGCCCGGCGACGGCGACGCGTTCCTGCGCGCGAGCGGCGCGGGCGAGCGATTGCTGTTCTCGGCGGTCGATCTCGG
GAATTTCCTCGAACCGCGCATCGAGTTGCCCGCGTCCGTCGAGGTCGAACTGGCGGCCGTCGTCCGGCTGCTCGTGCATC
ATCGCTGGCCGTTCCGGCTCCAGGCGACCTACGACGAATCGATCGGCCGCTTTCTCGACGTGTTCGAAGCGGTGGATCGC
GACACGCCGTTCGACGGGCTGCGCTGGTGCTTCGACTGCTGCGAGACCATTTCGGACGCCAATATCGCGCGGGTCGCGGC
GCTGGGCGGCGGCATCACCGTGCAGCTGCGCATGGCGTTCCAGGGCGAAGCGTTCATCGCGCGCCATGGTGCGGCGGCGG
CCACGCGCGCGCCGCCGCTCCGCGCGATGCTCGCGGCCGGGCTGCCGGTCGGCGCGGGGTCCGGCGCGACGGGCGCGGCG
AGCTACAACCCGTTCGTCGCGCTGTACTGGATGGTGTCGGGGCGCAGCGTGGGCGGCACCGCGCTGTATCCGGCGCGCAA
CCGGCTCGGCCGGATGGAGGCGCTGCGCCGCTACACGGCCGGCAGCGCGTGGTTCTCCGCGGACGAGCATCGCAAGGGCG
CGCTGGTGCCCGGCCGGTACGCCGATTTCGCGGTGCTGAGCGACGATTACTTCACGATCGATGTGCGGCGCATCCCGACG
CTGTCGTCGGTGCTGACGGTCGTCGACGGCAGGGTCGTGCACGCGGAACAGGAATTCTCGCCGCTTGCGCCGCCGCCGCC
GCCCGTCAGCCCTGCGTGGTCGCCGGTCGCCGCAGACGGCGCGGTGGGCGCCGACGTGCGCGACCGTTCGCATGCGTCGG
CGTCGGGCGACGCGTGCGGGGTGCCCGGCGTCGATGCGTGCCGTGCGCGCGGTGATGGACGCCGCTTCGCGCGACCGCGC
TATCCGACGGCAAGCGCGGCCGCCGAGCGCTGCGAAGCCTTCGGCCGCTGCTGCGTCGCGTTTTGA

Upstream 100 bases:

>100_bases
CCTGACGCGCGCGCGACGCGACACCGCCGACGCGGCGCAGCAGGCGGCATGACGATGCGCGAACCCGGTACGGCCGCGCG
GCCGCGCGATCGCAGCATGC

Downstream 100 bases:

>100_bases
GCGGCGCGTGCCGGTGCGCGACGCGCTGCGCGCCGGGTGTCGCCGTGCATCGAACGATTTTCCGCCCCGTGCACGGGGCA
TGAAGGAGACAGGATGAATG

Product: amidohydrolase 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 661; Mature: 660

Protein sequence:

>661_residues
MPMRAVRPLSPVRRPAGRPAPRRAQARTGGPTVLRATEPARSADLVVFNGKIATQDEQRSFVRALAVKDGTIVATGDDRD
VLRHAHDDTACIDLHGRTVIPGLIDAHLQAIRGGMNFNLEVRWDGVPSLADALELLGRQVRRTPAPHWARVAGGWTALQF
AEKRGPTTAELNAIAPDTPVFVQHLADSAWLNAAALRALGYGRDTPDPPGGELRRDRHGRPTGLLLARQDPAVLDAVLAR
APTLDAADRVNSTRQFMHALNRVGVTSAIDAGGDGLAYPDDYAAVATLARRGELTTRIAYALGARHAGRELDDFAKWIAL
TAPGDGDAFLRASGAGERLLFSAVDLGNFLEPRIELPASVEVELAAVVRLLVHHRWPFRLQATYDESIGRFLDVFEAVDR
DTPFDGLRWCFDCCETISDANIARVAALGGGITVQLRMAFQGEAFIARHGAAAATRAPPLRAMLAAGLPVGAGSGATGAA
SYNPFVALYWMVSGRSVGGTALYPARNRLGRMEALRRYTAGSAWFSADEHRKGALVPGRYADFAVLSDDYFTIDVRRIPT
LSSVLTVVDGRVVHAEQEFSPLAPPPPPVSPAWSPVAADGAVGADVRDRSHASASGDACGVPGVDACRARGDGRRFARPR
YPTASAAAERCEAFGRCCVAF

Sequences:

>Translated_661_residues
MPMRAVRPLSPVRRPAGRPAPRRAQARTGGPTVLRATEPARSADLVVFNGKIATQDEQRSFVRALAVKDGTIVATGDDRD
VLRHAHDDTACIDLHGRTVIPGLIDAHLQAIRGGMNFNLEVRWDGVPSLADALELLGRQVRRTPAPHWARVAGGWTALQF
AEKRGPTTAELNAIAPDTPVFVQHLADSAWLNAAALRALGYGRDTPDPPGGELRRDRHGRPTGLLLARQDPAVLDAVLAR
APTLDAADRVNSTRQFMHALNRVGVTSAIDAGGDGLAYPDDYAAVATLARRGELTTRIAYALGARHAGRELDDFAKWIAL
TAPGDGDAFLRASGAGERLLFSAVDLGNFLEPRIELPASVEVELAAVVRLLVHHRWPFRLQATYDESIGRFLDVFEAVDR
DTPFDGLRWCFDCCETISDANIARVAALGGGITVQLRMAFQGEAFIARHGAAAATRAPPLRAMLAAGLPVGAGSGATGAA
SYNPFVALYWMVSGRSVGGTALYPARNRLGRMEALRRYTAGSAWFSADEHRKGALVPGRYADFAVLSDDYFTIDVRRIPT
LSSVLTVVDGRVVHAEQEFSPLAPPPPPVSPAWSPVAADGAVGADVRDRSHASASGDACGVPGVDACRARGDGRRFARPR
YPTASAAAERCEAFGRCCVAF
>Mature_660_residues
PMRAVRPLSPVRRPAGRPAPRRAQARTGGPTVLRATEPARSADLVVFNGKIATQDEQRSFVRALAVKDGTIVATGDDRDV
LRHAHDDTACIDLHGRTVIPGLIDAHLQAIRGGMNFNLEVRWDGVPSLADALELLGRQVRRTPAPHWARVAGGWTALQFA
EKRGPTTAELNAIAPDTPVFVQHLADSAWLNAAALRALGYGRDTPDPPGGELRRDRHGRPTGLLLARQDPAVLDAVLARA
PTLDAADRVNSTRQFMHALNRVGVTSAIDAGGDGLAYPDDYAAVATLARRGELTTRIAYALGARHAGRELDDFAKWIALT
APGDGDAFLRASGAGERLLFSAVDLGNFLEPRIELPASVEVELAAVVRLLVHHRWPFRLQATYDESIGRFLDVFEAVDRD
TPFDGLRWCFDCCETISDANIARVAALGGGITVQLRMAFQGEAFIARHGAAAATRAPPLRAMLAAGLPVGAGSGATGAAS
YNPFVALYWMVSGRSVGGTALYPARNRLGRMEALRRYTAGSAWFSADEHRKGALVPGRYADFAVLSDDYFTIDVRRIPTL
SSVLTVVDGRVVHAEQEFSPLAPPPPPVSPAWSPVAADGAVGADVRDRSHASASGDACGVPGVDACRARGDGRRFARPRY
PTASAAAERCEAFGRCCVAF

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 70640; Mature: 70508

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMRAVRPLSPVRRPAGRPAPRRAQARTGGPTVLRATEPARSADLVVFNGKIATQDEQRS
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEECCEECCCHHHHH
FVRALAVKDGTIVATGDDRDVLRHAHDDTACIDLHGRTVIPGLIDAHLQAIRGGMNFNLE
HHHHHHCCCCEEEEECCCHHHHHHCCCCCEEEEECCCEECCHHHHHHHHHHHCCCCEEEE
VRWDGVPSLADALELLGRQVRRTPAPHWARVAGGWTALQFAEKRGPTTAELNAIAPDTPV
EEECCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHCCCCCEEECCCCCCCHH
FVQHLADSAWLNAAALRALGYGRDTPDPPGGELRRDRHGRPTGLLLARQDPAVLDAVLAR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHC
APTLDAADRVNSTRQFMHALNRVGVTSAIDAGGDGLAYPDDYAAVATLARRGELTTRIAY
CCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHH
ALGARHAGRELDDFAKWIALTAPGDGDAFLRASGAGERLLFSAVDLGNFLEPRIELPASV
HHHHHHCCCCHHHHHHEEEEECCCCCCCEEEECCCCCEEEEEHHHHCCCCCCCCCCCCCC
EVELAAVVRLLVHHRWPFRLQATYDESIGRFLDVFEAVDRDTPFDGLRWCFDCCETISDA
CHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
NIARVAALGGGITVQLRMAFQGEAFIARHGAAAATRAPPLRAMLAAGLPVGAGSGATGAA
CCEEHEEECCCEEEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCC
SYNPFVALYWMVSGRSVGGTALYPARNRLGRMEALRRYTAGSAWFSADEHRKGALVPGRY
CCCCEEEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
ADFAVLSDDYFTIDVRRIPTLSSVLTVVDGRVVHAEQEFSPLAPPPPPVSPAWSPVAADG
CCEEEECCCEEEEEEECCCCHHHHHHHHCCEEEECHHCCCCCCCCCCCCCCCCCCCCCCC
AVGADVRDRSHASASGDACGVPGVDACRARGDGRRFARPRYPTASAAAERCEAFGRCCVA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHECC
F
C
>Mature Secondary Structure 
PMRAVRPLSPVRRPAGRPAPRRAQARTGGPTVLRATEPARSADLVVFNGKIATQDEQRS
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEECCEECCCHHHHH
FVRALAVKDGTIVATGDDRDVLRHAHDDTACIDLHGRTVIPGLIDAHLQAIRGGMNFNLE
HHHHHHCCCCEEEEECCCHHHHHHCCCCCEEEEECCCEECCHHHHHHHHHHHCCCCEEEE
VRWDGVPSLADALELLGRQVRRTPAPHWARVAGGWTALQFAEKRGPTTAELNAIAPDTPV
EEECCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHCCCCCEEECCCCCCCHH
FVQHLADSAWLNAAALRALGYGRDTPDPPGGELRRDRHGRPTGLLLARQDPAVLDAVLAR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHC
APTLDAADRVNSTRQFMHALNRVGVTSAIDAGGDGLAYPDDYAAVATLARRGELTTRIAY
CCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHH
ALGARHAGRELDDFAKWIALTAPGDGDAFLRASGAGERLLFSAVDLGNFLEPRIELPASV
HHHHHHCCCCHHHHHHEEEEECCCCCCCEEEECCCCCEEEEEHHHHCCCCCCCCCCCCCC
EVELAAVVRLLVHHRWPFRLQATYDESIGRFLDVFEAVDRDTPFDGLRWCFDCCETISDA
CHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
NIARVAALGGGITVQLRMAFQGEAFIARHGAAAATRAPPLRAMLAAGLPVGAGSGATGAA
CCEEHEEECCCEEEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCC
SYNPFVALYWMVSGRSVGGTALYPARNRLGRMEALRRYTAGSAWFSADEHRKGALVPGRY
CCCCEEEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
ADFAVLSDDYFTIDVRRIPTLSSVLTVVDGRVVHAEQEFSPLAPPPPPVSPAWSPVAADG
CCEEEECCCEEEEEEECCCCHHHHHHHHCCEEEECHHCCCCCCCCCCCCCCCCCCCCCCC
AVGADVRDRSHASASGDACGVPGVDACRARGDGRRFARPRYPTASAAAERCEAFGRCCVA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHECC
F
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA