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The map label for this gene is thcD [H]

Identifier: 107022949

GI number: 107022949

Start: 1535195

End: 1536724

Strand: Reverse

Name: thcD [H]

Synonym: Bcen_1397

Alternate gene names: 107022949

Gene position: 1536724-1535195 (Counterclockwise)

Preceding gene: 107022951

Following gene: 107022948

Centisome position: 46.64

GC content: 70.98

Gene sequence:

>1530_bases
ATGACTGGAAGCGATGCGTCCTCACGCCCCGATCTCACGCAAGGGATCGCGCTCGACGATCTCGCCGACGGCGCGATGAT
CGAGGGCCATGTCGGCGACGCGGCGGTGCTGCTCGTGCGCCGTGCCGACGAACTGTTCGCGGTGGGCGCGCAATGCCCGC
ACTACGGCGGGCCGCTGGCCCAGGGCCTGCTGGTCGGCGACACGATTCGCTGTCCGTGGCATCACGCGGCGTTCTGCCTG
CGCACGGGCGAGATGGAGCGCGCGCCGGCGCTCGACGGGCTGCCGTGCTGGCGCGTCGAGCGGCGCAACGGCCGCGCGGT
CGTAGTCGATGCGCGGCCGGCCGCTGCGCCGCCTGCGCTGAAGGAAACCGGGCTGCCCACGTCGGTCGTGATCGTCGGCG
GCGGCGCCGCCGCGATCGCAGCAGCGGTGACGCTGCGGCAGGAAGGCTATCCGCACGCGATCACGCTGCTGAGCGCCGAT
GTCGATCCGCCGTACGACCGGCCGAATCTGTCGAAGGATTACCTCGCGGGCACGGCGGAAGCCGACTGGTTGCCGCTGCG
CGCGCCGTCGTTCTATGCCGGGCAGCGGATCGACGTGCGCTGCAATACGCGGGTCACGCGCATCGATCCCGCTCAGCGGT
CGGTCGAGCTGGCCGACGGCAGCCGTATCGAATACGGCGCGCTGCTGCTCGCGACCGGCGCCGAGCCGAACCGCCTGAAC
GTGCCCGGCGCCGATCTGCCGCACGTCCGTACGCTGCGCTCGCGCGCCGATTGCGATGCGCTGATCGCGAAGCTGAAAAC
CGCGCGCCGCTGCGTGGTGGTCGGCGCGAGCTTCATCGGCCTCGAAGCGGCTGCCGCGTTGCGTACACGCGGGCTCGACG
TGCACGTCGTCGCGCCCGATCCGCATCCGATGGGCCGCGTGCTCGGCGACGCGCTCGGCGACACGATCAAGGCGCTGCAC
GAAGCGCACGGCGTGGTGTTCCATCTCGGCGCGACGCCCGCGCGGATCGGGCCGGACAGCGTGACGCTGTCGAGTGGCGA
CGTGCTGCCGGCCGACGTCGTGCTGGTGGGGATCGGCGTGCATCCGAACGTCGAACTCGCGCAGGACGCCGGGCTCGCGG
TCGAACGCGGCGTGACGGTCGACCGCTTCCTGCAGACGAGTGCGGCCGGCATCTACGCGGCCGGCGATATCGCGCGCTGG
CCCGATCCGCTGACGGGCGAGCGGATTCGCGTCGAGCACTGGGTCGTCGCCGAGCGGCAGGGCATCGTCGCGGCGCGCAA
CCTGCTCGGCCAGCAGCGGCCGTTCGACGCGGTGCCGTTTTTCTGGAGTCAGCATTACGACCTGACGCTTCGCTATGTCG
GGCATGCGGAGCAATGGGATCGCGTCGAGATTGACGGCGACCTCGGCGCGCACGACTGCTCGATCGCGTACTGGCGTGGC
AACACGCGGCTCGCGGTCGTGACGATAGCTCGCGATCTCGACAACCTGAAGGCCGAAGCGGCGCTCGAGCGGCAGAGGGC
TTCCGCATGA

Upstream 100 bases:

>100_bases
TACAGTCCGCGTTTGCGTGCGCTGCGGGGCGTCTGTACCTGTTCTTTTCAGGAGGGATGGCGATAATGGGATCGTTACCT
GACTTTTCGGGAGCCACGTC

Downstream 100 bases:

>100_bases
AAGCAGCATGACGACACCCACCGAACCTTGAATGGAGGCGAAATGAACCCTGACGTCCGTGCCCGTTTCGAAACCGAGTT
TGCGCCGCGCATCGCGACGC

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MTGSDASSRPDLTQGIALDDLADGAMIEGHVGDAAVLLVRRADELFAVGAQCPHYGGPLAQGLLVGDTIRCPWHHAAFCL
RTGEMERAPALDGLPCWRVERRNGRAVVVDARPAAAPPALKETGLPTSVVIVGGGAAAIAAAVTLRQEGYPHAITLLSAD
VDPPYDRPNLSKDYLAGTAEADWLPLRAPSFYAGQRIDVRCNTRVTRIDPAQRSVELADGSRIEYGALLLATGAEPNRLN
VPGADLPHVRTLRSRADCDALIAKLKTARRCVVVGASFIGLEAAAALRTRGLDVHVVAPDPHPMGRVLGDALGDTIKALH
EAHGVVFHLGATPARIGPDSVTLSSGDVLPADVVLVGIGVHPNVELAQDAGLAVERGVTVDRFLQTSAAGIYAAGDIARW
PDPLTGERIRVEHWVVAERQGIVAARNLLGQQRPFDAVPFFWSQHYDLTLRYVGHAEQWDRVEIDGDLGAHDCSIAYWRG
NTRLAVVTIARDLDNLKAEAALERQRASA

Sequences:

>Translated_509_residues
MTGSDASSRPDLTQGIALDDLADGAMIEGHVGDAAVLLVRRADELFAVGAQCPHYGGPLAQGLLVGDTIRCPWHHAAFCL
RTGEMERAPALDGLPCWRVERRNGRAVVVDARPAAAPPALKETGLPTSVVIVGGGAAAIAAAVTLRQEGYPHAITLLSAD
VDPPYDRPNLSKDYLAGTAEADWLPLRAPSFYAGQRIDVRCNTRVTRIDPAQRSVELADGSRIEYGALLLATGAEPNRLN
VPGADLPHVRTLRSRADCDALIAKLKTARRCVVVGASFIGLEAAAALRTRGLDVHVVAPDPHPMGRVLGDALGDTIKALH
EAHGVVFHLGATPARIGPDSVTLSSGDVLPADVVLVGIGVHPNVELAQDAGLAVERGVTVDRFLQTSAAGIYAAGDIARW
PDPLTGERIRVEHWVVAERQGIVAARNLLGQQRPFDAVPFFWSQHYDLTLRYVGHAEQWDRVEIDGDLGAHDCSIAYWRG
NTRLAVVTIARDLDNLKAEAALERQRASA
>Mature_508_residues
TGSDASSRPDLTQGIALDDLADGAMIEGHVGDAAVLLVRRADELFAVGAQCPHYGGPLAQGLLVGDTIRCPWHHAAFCLR
TGEMERAPALDGLPCWRVERRNGRAVVVDARPAAAPPALKETGLPTSVVIVGGGAAAIAAAVTLRQEGYPHAITLLSADV
DPPYDRPNLSKDYLAGTAEADWLPLRAPSFYAGQRIDVRCNTRVTRIDPAQRSVELADGSRIEYGALLLATGAEPNRLNV
PGADLPHVRTLRSRADCDALIAKLKTARRCVVVGASFIGLEAAAALRTRGLDVHVVAPDPHPMGRVLGDALGDTIKALHE
AHGVVFHLGATPARIGPDSVTLSSGDVLPADVVLVGIGVHPNVELAQDAGLAVERGVTVDRFLQTSAAGIYAAGDIARWP
DPLTGERIRVEHWVVAERQGIVAARNLLGQQRPFDAVPFFWSQHYDLTLRYVGHAEQWDRVEIDGDLGAHDCSIAYWRGN
TRLAVVTIARDLDNLKAEAALERQRASA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=494, Percent_Identity=34.412955465587, Blast_Score=284, Evalue=2e-76,
Organism=Homo sapiens, GI65787454, Length=494, Percent_Identity=34.412955465587, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI226437568, Length=494, Percent_Identity=34.412955465587, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI4757732, Length=328, Percent_Identity=28.3536585365854, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI22202629, Length=328, Percent_Identity=28.3536585365854, Blast_Score=103, Evalue=5e-22,
Organism=Escherichia coli, GI1788892, Length=330, Percent_Identity=31.5151515151515, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1789065, Length=317, Percent_Identity=28.7066246056782, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789765, Length=293, Percent_Identity=27.9863481228669, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17559934, Length=467, Percent_Identity=29.7644539614561, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI281359715, Length=515, Percent_Identity=32.4271844660194, Blast_Score=251, Evalue=1e-66,
Organism=Drosophila melanogaster, GI281359713, Length=515, Percent_Identity=32.4271844660194, Blast_Score=251, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24639250, Length=515, Percent_Identity=32.4271844660194, Blast_Score=251, Evalue=1e-66,
Organism=Drosophila melanogaster, GI18543267, Length=515, Percent_Identity=32.4271844660194, Blast_Score=251, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24639252, Length=515, Percent_Identity=32.4271844660194, Blast_Score=251, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24639257, Length=515, Percent_Identity=32.4271844660194, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24585130, Length=458, Percent_Identity=31.0043668122271, Blast_Score=222, Evalue=6e-58,
Organism=Drosophila melanogaster, GI24581020, Length=371, Percent_Identity=29.1105121293801, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI28573993, Length=371, Percent_Identity=29.1105121293801, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 54263; Mature: 54132

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSDASSRPDLTQGIALDDLADGAMIEGHVGDAAVLLVRRADELFAVGAQCPHYGGPLA
CCCCCCCCCCCHHHCCCHHHCCCCCEEECCCCCEEEEEEECCCHHEEECCCCCCCCCHHH
QGLLVGDTIRCPWHHAAFCLRTGEMERAPALDGLPCWRVERRNGRAVVVDARPAAAPPAL
CCEEECCEEECCCHHHEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCH
KETGLPTSVVIVGGGAAAIAAAVTLRQEGYPHAITLLSADVDPPYDRPNLSKDYLAGTAE
HHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHCCCCC
ADWLPLRAPSFYAGQRIDVRCNTRVTRIDPAQRSVELADGSRIEYGALLLATGAEPNRLN
CCEEECCCCCCCCCCEEEEEECCEEEEECCCHHEEEECCCCEEEEEEEEEEECCCCCEEC
VPGADLPHVRTLRSRADCDALIAKLKTARRCVVVGASFIGLEAAAALRTRGLDVHVVAPD
CCCCCCCHHHHHHHHCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHCCCEEEEECCC
PHPMGRVLGDALGDTIKALHEAHGVVFHLGATPARIGPDSVTLSSGDVLPADVVLVGIGV
CCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEECCCCCCCCEEEEEEECC
HPNVELAQDAGLAVERGVTVDRFLQTSAAGIYAAGDIARWPDPLTGERIRVEHWVVAERQ
CCCCEECCCCCCEEECCCCHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEEEEEECCC
GIVAARNLLGQQRPFDAVPFFWSQHYDLTLRYVGHAEQWDRVEIDGDLGAHDCSIAYWRG
CHHHHHHHHCCCCCCCCCCHHHCCCCEEEEEEECCCCCCCEEEECCCCCCCCCEEEEEEC
NTRLAVVTIARDLDNLKAEAALERQRASA
CCEEEEEEEEHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGSDASSRPDLTQGIALDDLADGAMIEGHVGDAAVLLVRRADELFAVGAQCPHYGGPLA
CCCCCCCCCCHHHCCCHHHCCCCCEEECCCCCEEEEEEECCCHHEEECCCCCCCCCHHH
QGLLVGDTIRCPWHHAAFCLRTGEMERAPALDGLPCWRVERRNGRAVVVDARPAAAPPAL
CCEEECCEEECCCHHHEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCH
KETGLPTSVVIVGGGAAAIAAAVTLRQEGYPHAITLLSADVDPPYDRPNLSKDYLAGTAE
HHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHCCCCC
ADWLPLRAPSFYAGQRIDVRCNTRVTRIDPAQRSVELADGSRIEYGALLLATGAEPNRLN
CCEEECCCCCCCCCCEEEEEECCEEEEECCCHHEEEECCCCEEEEEEEEEEECCCCCEEC
VPGADLPHVRTLRSRADCDALIAKLKTARRCVVVGASFIGLEAAAALRTRGLDVHVVAPD
CCCCCCCHHHHHHHHCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHCCCEEEEECCC
PHPMGRVLGDALGDTIKALHEAHGVVFHLGATPARIGPDSVTLSSGDVLPADVVLVGIGV
CCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEECCCCCCCCEEEEEEECC
HPNVELAQDAGLAVERGVTVDRFLQTSAAGIYAAGDIARWPDPLTGERIRVEHWVVAERQ
CCCCEECCCCCCEEECCCCHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEEEEEECCC
GIVAARNLLGQQRPFDAVPFFWSQHYDLTLRYVGHAEQWDRVEIDGDLGAHDCSIAYWRG
CHHHHHHHHCCCCCCCCCCHHHCCCCEEEEEEECCCCCCCEEEECCCCCCCCCEEEEEEC
NTRLAVVTIARDLDNLKAEAALERQRASA
CCEEEEEEEEHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]