| Definition | Burkholderia cenocepacia AU 1054 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_008060 |
| Length | 3,294,563 |
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The map label for this gene is rpoN [H]
Identifier: 107022904
GI number: 107022904
Start: 1492813
End: 1494246
Strand: Reverse
Name: rpoN [H]
Synonym: Bcen_1352
Alternate gene names: 107022904
Gene position: 1494246-1492813 (Counterclockwise)
Preceding gene: 107022905
Following gene: 107022903
Centisome position: 45.35
GC content: 68.97
Gene sequence:
>1434_bases ATGCCCGTCTCGCTTGCGCTGCAGATGCGACAGCATCTCGCACTTACGCCGAGGCTTCAGCAGTCGCTGCGGCTGCTGCA GTTGTCCTCGCTGGAGTTTCAACAGGAATTGCGTCAGGCGCTCGACATGAACCCGTTTCTCGAAGACGGCCAGGGCGACG AGGACACCGTTGCCGGCACGCCCGAGCAGCCGACCGAAGCGGCCGCGCCCGAAGCGGCGCCCGAGCACGACGACATTCGC CCGCCGGATGGCGAGGTGCCGTTTACCGCGGCGCCCGCGCCGCGCGGCACGAGCCGTCAGGGCGACGATACCGACGGCCT GTCGCCCGGCGAATGGATCGCCGCCGAGCCGTCGCTGCATCAGCAACTGCACGATGCGCTGCGGCTCTATCCGCTCAACA AGCGCGACCGCGAAGCCGCGCGCCTCGTGATCGATGCGCTGGACGACGACGGCTACCTGCGTCAGGAACTGCCTGAACTG CTGGTCGCCGCCGATCCCGCGTGGCTGCTGTCGGAGCAGGATCTCGCGGTCGCGCTGCGGCTCGTGCAGATGCTCGACCG GCCGGGTATCGCGGCCCGTTCGTTGTCCGAATGCCTGGTGTTGCAGCTCGACGCGATTCCGGCCGGCACGCCGGCGCTCG CGGAAGCGAAGGCGATCGCGCGCGAGCATCTGGAGCGGCTCGCGCGCCGCGAGACGGCCGAGATCCAGCGACGCCTCGGC TGCAACCAGCAGACGCTGCACGCGGCCTGCGCGCTGGTGCGCGGGCTCGACCCGCGCCCGGGCAATCACTACGGCAGCAC GCGCGGCGACTACGTGGTGCCCGACGTGATCGTGCGTCAGGTGCGCGACGAATGGATCGTCACGATCAATCCGGCCGTGC TGCCGCGCGCCCGCCTGCACGAGCGCTATGCGACGCTGTTCGCGCAGTCGAGCGGCGAGCGCGATTCGCCGCTCGGCCAG CAGTTGCAGGAAGCGCGCTGGCTGATCCGCAACGTGCAGAAGCGTTTCGACACGATTCAGCGCGTGGGCGAATGCATCGT CGCGCGGCAGCGCGATTTCTTCCGCTACGGCGAGATCGCGATGAAGCCGTTGGTGCTGCGCGACATCGCGGAAGAGCTCG ACCTGCACGAATCGACCGTCTCGCGCGCGACCGGCAACAAGTACATGGCCACGCCGCACGGCACGTTCGAGTTCAAGCAT TTCTTCCCGCGCAAGCTGGAAGCAGCCGGCAAGGGCGTGTGTTCGGCGTCGGCCGCGAAGGTGCTGATCCGCGACATGAT CGCGGCCGAGCGGCAGACCGATCCGCTGTCCGACGTCGCGCTCGCGGAGAACCTCGCGGGCCGCGGCATCCTTCTTGCGC GCCGCACGGTGACCAAGTATCGCCAGGCGATGAAGATTCCGCCGGCCGACTTGCGGCGCCGCGACGCCGCGTGA
Upstream 100 bases:
>100_bases CGCGTCGCAGCGCGCTTCGCGCGACTGGCACGGAAGCTGCGTGACGTTCGGCAGAAGACATACCCGCGAACCCGCGAACC GAACCGTCAGGAGGCTTTCG
Downstream 100 bases:
>100_bases CGGCCTCACTGTTCCAACCCCGCGCGTGCCGTCTCTCGCGTCGGCTCGCGCGCCAACGAGAGGAGCGCAACCATGCCAGC CAAATCCCAGGCCCAGCAGC
Product: RNA polymerase factor sigma-54
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 477; Mature: 476
Protein sequence:
>477_residues MPVSLALQMRQHLALTPRLQQSLRLLQLSSLEFQQELRQALDMNPFLEDGQGDEDTVAGTPEQPTEAAAPEAAPEHDDIR PPDGEVPFTAAPAPRGTSRQGDDTDGLSPGEWIAAEPSLHQQLHDALRLYPLNKRDREAARLVIDALDDDGYLRQELPEL LVAADPAWLLSEQDLAVALRLVQMLDRPGIAARSLSECLVLQLDAIPAGTPALAEAKAIAREHLERLARRETAEIQRRLG CNQQTLHAACALVRGLDPRPGNHYGSTRGDYVVPDVIVRQVRDEWIVTINPAVLPRARLHERYATLFAQSSGERDSPLGQ QLQEARWLIRNVQKRFDTIQRVGECIVARQRDFFRYGEIAMKPLVLRDIAEELDLHESTVSRATGNKYMATPHGTFEFKH FFPRKLEAAGKGVCSASAAKVLIRDMIAAERQTDPLSDVALAENLAGRGILLARRTVTKYRQAMKIPPADLRRRDAA
Sequences:
>Translated_477_residues MPVSLALQMRQHLALTPRLQQSLRLLQLSSLEFQQELRQALDMNPFLEDGQGDEDTVAGTPEQPTEAAAPEAAPEHDDIR PPDGEVPFTAAPAPRGTSRQGDDTDGLSPGEWIAAEPSLHQQLHDALRLYPLNKRDREAARLVIDALDDDGYLRQELPEL LVAADPAWLLSEQDLAVALRLVQMLDRPGIAARSLSECLVLQLDAIPAGTPALAEAKAIAREHLERLARRETAEIQRRLG CNQQTLHAACALVRGLDPRPGNHYGSTRGDYVVPDVIVRQVRDEWIVTINPAVLPRARLHERYATLFAQSSGERDSPLGQ QLQEARWLIRNVQKRFDTIQRVGECIVARQRDFFRYGEIAMKPLVLRDIAEELDLHESTVSRATGNKYMATPHGTFEFKH FFPRKLEAAGKGVCSASAAKVLIRDMIAAERQTDPLSDVALAENLAGRGILLARRTVTKYRQAMKIPPADLRRRDAA >Mature_476_residues PVSLALQMRQHLALTPRLQQSLRLLQLSSLEFQQELRQALDMNPFLEDGQGDEDTVAGTPEQPTEAAAPEAAPEHDDIRP PDGEVPFTAAPAPRGTSRQGDDTDGLSPGEWIAAEPSLHQQLHDALRLYPLNKRDREAARLVIDALDDDGYLRQELPELL VAADPAWLLSEQDLAVALRLVQMLDRPGIAARSLSECLVLQLDAIPAGTPALAEAKAIAREHLERLARRETAEIQRRLGC NQQTLHAACALVRGLDPRPGNHYGSTRGDYVVPDVIVRQVRDEWIVTINPAVLPRARLHERYATLFAQSSGERDSPLGQQ LQEARWLIRNVQKRFDTIQRVGECIVARQRDFFRYGEIAMKPLVLRDIAEELDLHESTVSRATGNKYMATPHGTFEFKHF FPRKLEAAGKGVCSASAAKVLIRDMIAAERQTDPLSDVALAENLAGRGILLARRTVTKYRQAMKIPPADLRRRDAA
Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]
COG id: COG1508
COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the sigma-54 factor family [H]
Homologues:
Organism=Escherichia coli, GI1789594, Length=479, Percent_Identity=38.2045929018789, Blast_Score=317, Evalue=1e-87,
Paralogues:
None
Copy number: 70 (log & stationary phase) [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000394 - InterPro: IPR007046 - InterPro: IPR007634 [H]
Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]
EC number: NA
Molecular weight: Translated: 53078; Mature: 52947
Theoretical pI: Translated: 6.16; Mature: 6.16
Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.0 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MPVSLALQMRQHLALTPRLQQSLRLLQLSSLEFQQELRQALDMNPFLEDGQGDEDTVAGT CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC PEQPTEAAAPEAAPEHDDIRPPDGEVPFTAAPAPRGTSRQGDDTDGLSPGEWIAAEPSLH CCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHH QQLHDALRLYPLNKRDREAARLVIDALDDDGYLRQELPELLVAADPAWLLSEQDLAVALR HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCCHHHHHHHH LVQMLDRPGIAARSLSECLVLQLDAIPAGTPALAEAKAIAREHLERLARRETAEIQRRLG HHHHHCCCCHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC CNQQTLHAACALVRGLDPRPGNHYGSTRGDYVVPDVIVRQVRDEWIVTINPAVLPRARLH CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECHHHHHHHHCCCEEEEECCHHCCHHHHH ERYATLFAQSSGERDSPLGQQLQEARWLIRNVQKRFDTIQRVGECIVARQRDFFRYGEIA HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH MKPLVLRDIAEELDLHESTVSRATGNKYMATPHGTFEFKHFFPRKLEAAGKGVCSASAAK HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHH VLIRDMIAAERQTDPLSDVALAENLAGRGILLARRTVTKYRQAMKIPPADLRRRDAA HHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCHHHHHCCCC >Mature Secondary Structure PVSLALQMRQHLALTPRLQQSLRLLQLSSLEFQQELRQALDMNPFLEDGQGDEDTVAGT CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC PEQPTEAAAPEAAPEHDDIRPPDGEVPFTAAPAPRGTSRQGDDTDGLSPGEWIAAEPSLH CCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHH QQLHDALRLYPLNKRDREAARLVIDALDDDGYLRQELPELLVAADPAWLLSEQDLAVALR HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCCHHHHHHHH LVQMLDRPGIAARSLSECLVLQLDAIPAGTPALAEAKAIAREHLERLARRETAEIQRRLG HHHHHCCCCHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC CNQQTLHAACALVRGLDPRPGNHYGSTRGDYVVPDVIVRQVRDEWIVTINPAVLPRARLH CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECHHHHHHHHCCCEEEEECCHHCCHHHHH ERYATLFAQSSGERDSPLGQQLQEARWLIRNVQKRFDTIQRVGECIVARQRDFFRYGEIA HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH MKPLVLRDIAEELDLHESTVSRATGNKYMATPHGTFEFKHFFPRKLEAAGKGVCSASAAK HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHH VLIRDMIAAERQTDPLSDVALAENLAGRGILLARRTVTKYRQAMKIPPADLRRRDAA HHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: DNA [C]
Specific reaction: Protein + DNA = Protein-DNA [C]
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8113171; 10984043 [H]