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The map label for this gene is ddh [H]

Identifier: 107021900

GI number: 107021900

Start: 377989

End: 378987

Strand: Reverse

Name: ddh [H]

Synonym: Bcen_0342

Alternate gene names: 107021900

Gene position: 378987-377989 (Counterclockwise)

Preceding gene: 107021901

Following gene: 107021899

Centisome position: 11.5

GC content: 67.37

Gene sequence:

>999_bases
GTGCGCGTGATCCTGTTCAGCAGCCGGCAGTACGACGACGATTCGTTCACCGCCGCCAACCGGCAGTTCGGCTATCGGCT
GCACTTCCAGCCGTCGCACCTCGACGCGGAAACGGCGATTCTCGCGCACGGCTATGACGTGGTCTGCCCGTTCGTCAACG
ACACCGTCGACGCGGCCGTGCTCGAACGGCTCGCCGACGGCGGCACGCGGCTGATCGCCCTGCGCTCGGCGGGCTTCAAC
CATGTCGACCTCGCCGCCGCCGAACGGCTCGGCATCACGGTCGTGCGCGTGCCCGCGTATTCGCCGCATGCGGTCGCCGA
GCACGCGGTCGCGCTGATCCTCGCACTCAATCGCCGCCTGCCGCGCGCGGTCGCCCGCACCCGCGAAGGCGACTTCTCAC
TGAACGGACTGCTGGGCTTCGACCTGCACGGCAAGACCGTCGGCGTGATCGGCACTGGCATCATCGGCAGCGTGTTCGCG
AAGATCATGATGGGCTTCGGGATGCACGTGCTCGCGCATTCGGTGCCGCCGTACAACGACGAGCTGATCGCGTTCGGTGC
GCGCTACGTCGAGCTCGACGAGCTGCTGCATCATGCCGACATCGTCAGCCTGCATTGCCCGCTGCTGCCGTCGACACACC
ACCTGATCAACGAGCGGACGCTCGCGCGGATGAAGCACGGCGCGATGCTGATCAACACCGGGCGTGGCGGCCTCGTCGAT
GCGCAGGCGCTGGTCGACGCGCTCAAGAGCGGCCAGCTCGGCCATCTCGGGCTCGACGTGTACGAGGAGGAAAGCGGGCT
CTTCTTCGAGGATCACTCCGACCTGCCGCTGCAGGACGACGTGCTCGCGCGCCTGCTGACGTTCCCGAACGTGATCGTCA
CATCGCACCAGGCGTTCTTCACGCGCGAGGCGCTGGCCGAGATCGCACACACGACGCTGCTGAACATCGAGGCGTGGCAT
GCGGGCGCGCCCGCGAACGTCGTGATCGCAAACCGCTGA

Upstream 100 bases:

>100_bases
GGCGGATTACGGCTTCCTGCGGCCGGTACGCGGCCCCGAACGCTCCGACATCGTCACGGGGCTGCAGTAAACTGCGCGCG
TCAGCCATCGGAGGTGCAGC

Downstream 100 bases:

>100_bases
GAAACCGCACGTTTGATGGCGTGCAGCGCATCCGCAATCGTTTGCTCGTGCATGCTGCCGCACACCGCACTCGCCCTCGT
CAACAATTCAGCGCATTGTT

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MRVILFSSRQYDDDSFTAANRQFGYRLHFQPSHLDAETAILAHGYDVVCPFVNDTVDAAVLERLADGGTRLIALRSAGFN
HVDLAAAERLGITVVRVPAYSPHAVAEHAVALILALNRRLPRAVARTREGDFSLNGLLGFDLHGKTVGVIGTGIIGSVFA
KIMMGFGMHVLAHSVPPYNDELIAFGARYVELDELLHHADIVSLHCPLLPSTHHLINERTLARMKHGAMLINTGRGGLVD
AQALVDALKSGQLGHLGLDVYEEESGLFFEDHSDLPLQDDVLARLLTFPNVIVTSHQAFFTREALAEIAHTTLLNIEAWH
AGAPANVVIANR

Sequences:

>Translated_332_residues
MRVILFSSRQYDDDSFTAANRQFGYRLHFQPSHLDAETAILAHGYDVVCPFVNDTVDAAVLERLADGGTRLIALRSAGFN
HVDLAAAERLGITVVRVPAYSPHAVAEHAVALILALNRRLPRAVARTREGDFSLNGLLGFDLHGKTVGVIGTGIIGSVFA
KIMMGFGMHVLAHSVPPYNDELIAFGARYVELDELLHHADIVSLHCPLLPSTHHLINERTLARMKHGAMLINTGRGGLVD
AQALVDALKSGQLGHLGLDVYEEESGLFFEDHSDLPLQDDVLARLLTFPNVIVTSHQAFFTREALAEIAHTTLLNIEAWH
AGAPANVVIANR
>Mature_332_residues
MRVILFSSRQYDDDSFTAANRQFGYRLHFQPSHLDAETAILAHGYDVVCPFVNDTVDAAVLERLADGGTRLIALRSAGFN
HVDLAAAERLGITVVRVPAYSPHAVAEHAVALILALNRRLPRAVARTREGDFSLNGLLGFDLHGKTVGVIGTGIIGSVFA
KIMMGFGMHVLAHSVPPYNDELIAFGARYVELDELLHHADIVSLHCPLLPSTHHLINERTLARMKHGAMLINTGRGGLVD
AQALVDALKSGQLGHLGLDVYEEESGLFFEDHSDLPLQDDVLARLLTFPNVIVTSHQAFFTREALAEIAHTTLLNIEAWH
AGAPANVVIANR

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=252, Percent_Identity=35.3174603174603, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI61743967, Length=249, Percent_Identity=37.3493975903614, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI4557497, Length=249, Percent_Identity=37.3493975903614, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI145580578, Length=249, Percent_Identity=35.3413654618474, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4557499, Length=249, Percent_Identity=35.3413654618474, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI145580575, Length=257, Percent_Identity=34.6303501945525, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI6912396, Length=277, Percent_Identity=31.4079422382672, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI1787645, Length=322, Percent_Identity=49.3788819875776, Blast_Score=324, Evalue=5e-90,
Organism=Escherichia coli, GI87082289, Length=306, Percent_Identity=31.3725490196078, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1789279, Length=258, Percent_Identity=32.5581395348837, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1788660, Length=211, Percent_Identity=31.7535545023697, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=302, Percent_Identity=28.476821192053, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI25147481, Length=242, Percent_Identity=28.099173553719, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320925, Length=233, Percent_Identity=34.7639484978541, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322116, Length=233, Percent_Identity=34.3347639484979, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324055, Length=203, Percent_Identity=35.4679802955665, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324964, Length=212, Percent_Identity=29.2452830188679, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6325144, Length=126, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321253, Length=130, Percent_Identity=36.1538461538462, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=27.8571428571429, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921140, Length=257, Percent_Identity=30.7392996108949, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24646446, Length=248, Percent_Identity=36.6935483870968, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24646448, Length=248, Percent_Identity=36.6935483870968, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24646452, Length=248, Percent_Identity=36.6935483870968, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24646450, Length=248, Percent_Identity=36.6935483870968, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI62472511, Length=249, Percent_Identity=37.3493975903614, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI28574286, Length=276, Percent_Identity=31.8840579710145, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI28571528, Length=283, Percent_Identity=32.5088339222615, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28574284, Length=260, Percent_Identity=30.7692307692308, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI45552429, Length=260, Percent_Identity=30.7692307692308, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24585514, Length=260, Percent_Identity=30.7692307692308, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI28574282, Length=260, Percent_Identity=30.7692307692308, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI45551003, Length=260, Percent_Identity=30.7692307692308, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24585516, Length=258, Percent_Identity=25.1937984496124, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28

Molecular weight: Translated: 36145; Mature: 36145

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVILFSSRQYDDDSFTAANRQFGYRLHFQPSHLDAETAILAHGYDVVCPFVNDTVDAAV
CEEEEECCCCCCCCCCEECCCCCCEEEEECCCCCCCHHEEEECCCEEEECCCCCHHHHHH
LERLADGGTRLIALRSAGFNHVDLAAAERLGITVVRVPAYSPHAVAEHAVALILALNRRL
HHHHHCCCEEEEEEECCCCCEEHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
PRAVARTREGDFSLNGLLGFDLHGKTVGVIGTGIIGSVFAKIMMGFGMHVLAHSVPPYND
HHHHHHCCCCCEEECCEEEEEECCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHCCCCCCC
ELIAFGARYVELDELLHHADIVSLHCPLLPSTHHLINERTLARMKHGAMLINTGRGGLVD
CEEEECCHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCEE
AQALVDALKSGQLGHLGLDVYEEESGLFFEDHSDLPLQDDVLARLLTFPNVIVTSHQAFF
HHHHHHHHHCCCCCEECCEEEECCCCCEEECCCCCCCCHHHHHHHHHCCCEEEECCHHHH
TREALAEIAHTTLLNIEAWHAGAPANVVIANR
HHHHHHHHHHHHEEEEEEECCCCCCEEEEECC
>Mature Secondary Structure
MRVILFSSRQYDDDSFTAANRQFGYRLHFQPSHLDAETAILAHGYDVVCPFVNDTVDAAV
CEEEEECCCCCCCCCCEECCCCCCEEEEECCCCCCCHHEEEECCCEEEECCCCCHHHHHH
LERLADGGTRLIALRSAGFNHVDLAAAERLGITVVRVPAYSPHAVAEHAVALILALNRRL
HHHHHCCCEEEEEEECCCCCEEHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
PRAVARTREGDFSLNGLLGFDLHGKTVGVIGTGIIGSVFAKIMMGFGMHVLAHSVPPYND
HHHHHHCCCCCEEECCEEEEEECCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHCCCCCCC
ELIAFGARYVELDELLHHADIVSLHCPLLPSTHHLINERTLARMKHGAMLINTGRGGLVD
CEEEECCHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCEE
AQALVDALKSGQLGHLGLDVYEEESGLFFEDHSDLPLQDDVLARLLTFPNVIVTSHQAFF
HHHHHHHHHCCCCCEECCEEEECCCCCEEECCCCCCCCHHHHHHHHHCCCEEEECCHHHH
TREALAEIAHTTLLNIEAWHAGAPANVVIANR
HHHHHHHHHHHHEEEEEEECCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8320209 [H]