Click here to switch to the map view.

The map label for this gene is panB

Identifier: 107021831

GI number: 107021831

Start: 297764

End: 298579

Strand: Reverse

Name: panB

Synonym: Bcen_0271

Alternate gene names: 107021831

Gene position: 298579-297764 (Counterclockwise)

Preceding gene: 107021832

Following gene: 107021825

Centisome position: 9.06

GC content: 67.89

Gene sequence:

>816_bases
ATGACCTATCTCCAGGAATCGAGCCGGCCTGCCGTGACGGTACCCAAGCTGCAGGCAATGCGCGACGCCGGCGAGAAGAT
CGCGATGCTCACGTGCTACGACGCGAGCTTTTCCGCGCTGCTCGATCGCGCCGGCACCGACGTGCTGCTGATCGGCGATT
CGCTCGGCAACGTGCTGCAGGGCCACACGACCACGCTGCCCGTGTCGATCGACGACATCGCGTACCACACCGCCTGTGTC
GCACGGGCGCAGCCGCGCGCGCTCGTCGTCGCCGATCTGCCGTTCGGCACGTACGGCACGCCGGTCGACGCGTTCGCGAA
CGCGGTCAAGCTGATGCGCGCGGGCGCGCAGATGGTCAAGCTCGAAGGCGGCGAATGGCTCGCCGACACGATCCGCTTCC
TCGTCGAACGCTCGGTGCCCGTCTGTGCGCACCTCGGCCTCACGCCGCAGTCGGTGCATGCGTTCGGCGGCTTCAAGGTT
CAGGGCAAGACCGAAGCCGGCGCCGCGCAACTGCTGCGCGATGCGCGCGCGATCGAGGATGCCGGCGCGCAGCTCGTCGT
GCTCGAAGCCGTGCCGACGCTCGTCGCGGCCGAAGTCACGCACATGCTGAAGATCCCGACGATCGGCATCGGCGCCGGCG
TCGACTGCTCGGGGCAGGTGCTCGTGCTGCACGACATGCTCGGCGTGTTCCCCGGCAAGCGGCCGCGTTTCGTGAAGGAT
TTCATGCAGGGGCAGCCGAACATTCAGGCGGCCGTCGAAGCCTACGTGAGCGCGGTGAAGGATCGATCGTTCCCCGGCCC
CGAACACTCGTTCTGA

Upstream 100 bases:

>100_bases
ATCGAGACGATGCGCGGCCGCAAGGAATTCTTCGTCAAGGGCGAGACGACGCGCTGACGCGTCCCGCCTGTCGCTCTACC
CCCATCGACCCGGATTTCCC

Downstream 100 bases:

>100_bases
CGTGGAAGACCGGATGTAAAAAAGCGCGGCGCCCTTTGCGGGGCGCCGCGCTTTTTTGTTTGTGCGCCGCGTGGATGAAC
GAGGCACCGGAGCAAGCATA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase 1; KPHMT 1

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTYLQESSRPAVTVPKLQAMRDAGEKIAMLTCYDASFSALLDRAGTDVLLIGDSLGNVLQGHTTTLPVSIDDIAYHTACV
ARAQPRALVVADLPFGTYGTPVDAFANAVKLMRAGAQMVKLEGGEWLADTIRFLVERSVPVCAHLGLTPQSVHAFGGFKV
QGKTEAGAAQLLRDARAIEDAGAQLVVLEAVPTLVAAEVTHMLKIPTIGIGAGVDCSGQVLVLHDMLGVFPGKRPRFVKD
FMQGQPNIQAAVEAYVSAVKDRSFPGPEHSF

Sequences:

>Translated_271_residues
MTYLQESSRPAVTVPKLQAMRDAGEKIAMLTCYDASFSALLDRAGTDVLLIGDSLGNVLQGHTTTLPVSIDDIAYHTACV
ARAQPRALVVADLPFGTYGTPVDAFANAVKLMRAGAQMVKLEGGEWLADTIRFLVERSVPVCAHLGLTPQSVHAFGGFKV
QGKTEAGAAQLLRDARAIEDAGAQLVVLEAVPTLVAAEVTHMLKIPTIGIGAGVDCSGQVLVLHDMLGVFPGKRPRFVKD
FMQGQPNIQAAVEAYVSAVKDRSFPGPEHSF
>Mature_270_residues
TYLQESSRPAVTVPKLQAMRDAGEKIAMLTCYDASFSALLDRAGTDVLLIGDSLGNVLQGHTTTLPVSIDDIAYHTACVA
RAQPRALVVADLPFGTYGTPVDAFANAVKLMRAGAQMVKLEGGEWLADTIRFLVERSVPVCAHLGLTPQSVHAFGGFKVQ
GKTEAGAAQLLRDARAIEDAGAQLVVLEAVPTLVAAEVTHMLKIPTIGIGAGVDCSGQVLVLHDMLGVFPGKRPRFVKDF
MQGQPNIQAAVEAYVSAVKDRSFPGPEHSF

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=259, Percent_Identity=53.2818532818533, Blast_Score=286, Evalue=7e-79,
Organism=Saccharomyces cerevisiae, GI6319653, Length=278, Percent_Identity=35.9712230215827, Blast_Score=180, Evalue=2e-46,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB1_BURCH (A0K4T1)

Other databases:

- EMBL:   CP000458
- RefSeq:   YP_834401.1
- ProteinModelPortal:   A0K4T1
- SMR:   A0K4T1
- STRING:   A0K4T1
- GeneID:   4450580
- GenomeReviews:   CP000458_GR
- KEGG:   bch:Bcen2424_0755
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   BCEN331272:BCEN2424_0755-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28753; Mature: 28621

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: ACT_SITE 189-189 BINDING 92-92 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYLQESSRPAVTVPKLQAMRDAGEKIAMLTCYDASFSALLDRAGTDVLLIGDSLGNVLQ
CCCCCCCCCCCEECHHHHHHHCCCCEEEEEEEECCHHHHHHHHCCCCEEEECCCHHHHHC
GHTTTLPVSIDDIAYHTACVARAQPRALVVADLPFGTYGTPVDAFANAVKLMRAGAQMVK
CCCEEEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEE
LEGGEWLADTIRFLVERSVPVCAHLGLTPQSVHAFGGFKVQGKTEAGAAQLLRDARAIED
ECCCCHHHHHHHHHHHCCCCCEEECCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHH
AGAQLVVLEAVPTLVAAEVTHMLKIPTIGIGAGVDCSGQVLVLHDMLGVFPGKRPRFVKD
CCCCEEEEHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEHHHHHCCCCCCHHHHH
FMQGQPNIQAAVEAYVSAVKDRSFPGPEHSF
HHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TYLQESSRPAVTVPKLQAMRDAGEKIAMLTCYDASFSALLDRAGTDVLLIGDSLGNVLQ
CCCCCCCCCCEECHHHHHHHCCCCEEEEEEEECCHHHHHHHHCCCCEEEECCCHHHHHC
GHTTTLPVSIDDIAYHTACVARAQPRALVVADLPFGTYGTPVDAFANAVKLMRAGAQMVK
CCCEEEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEE
LEGGEWLADTIRFLVERSVPVCAHLGLTPQSVHAFGGFKVQGKTEAGAAQLLRDARAIED
ECCCCHHHHHHHHHHHCCCCCEEECCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHH
AGAQLVVLEAVPTLVAAEVTHMLKIPTIGIGAGVDCSGQVLVLHDMLGVFPGKRPRFVKD
CCCCEEEEHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEHHHHHCCCCCCHHHHH
FMQGQPNIQAAVEAYVSAVKDRSFPGPEHSF
HHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA