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The map label for this gene is alsR [H]

Identifier: 107021717

GI number: 107021717

Start: 163966

End: 164856

Strand: Reverse

Name: alsR [H]

Synonym: Bcen_0157

Alternate gene names: 107021717

Gene position: 164856-163966 (Counterclockwise)

Preceding gene: 107021720

Following gene: 107021714

Centisome position: 5.0

GC content: 70.15

Gene sequence:

>891_bases
ATGGAACTCAAGCAATTGCGCGCCTTCGTCACGCTCGCGGAAGAACTGCATTTCGGGCGCGCCGCGCAGCGCCTTTGCAT
CGTGCAGCCCGCGCTGAGCATGCAGATCAAGGCGCTCGAGGCGGAACTCGGCGCGCGCCTGTTCGAGCGCGACCGGCACA
AGGTCGAACTGAGCGACACCGGGCGCGTGTTCCTGCCGGAAGCGCGCGCGACGCTGCAGCAGGCCGCGCGCGCGGAGCAG
TTGGCGCGGCTGTCGAGCCGCGGCGAAATCGGCACGCTGCGGATCGCGTTCGTATCGTCGGTGTTGCCCACGCTGCTGCC
GGCCGTGCTGCGCACGATGCGCGAGCGCTATCCGCTGATCGTGCTCGAACTGAAGGACATGCCGACGCCCGACCAGATCG
CCGCGCTGCGCGACCGGCGGATCGATTTCGGGATGATCCGGCTGCCGGCCGCGTATGCGGGCATCGACACGCGGGTGGTG
CTCGAGGAAGGCTTCGTGGTCGCGCTGCCGCTGGACCATCCGCTCGCCGCGTACGATACGATCGCGCCGGCCGCGTTGCG
CGACCACCCCGCGTTCGTGCTCGCGCGCCGCTACGCGCCCGGCTTTCACGACGACATGCTGCTCGCGCTGAGCCGCGCGG
GCACGACGCTCGAGATCGCGCAGGAATTCGGCGAATTCACGACGATGCTCGCGCTCGTCGCGGCGGGGATGGGGATCGGG
CTGATTCCGGCGCAGGCGGCCAACGCGCTGCCGCCGAACGTGCTCGCGCGACCGCTCGACCTGGCCGGTCACCGCAGCGG
CATCGGGCTCGCGTGGACCGATCTCGACAGCCCGCTGAAGCGCGCGTTCGTCGATGCGGTCGAGCACGTGATGGCGCACG
CCGGGCAATAA

Upstream 100 bases:

>100_bases
TTCACGCTATCTTCGGTCGCCGGCCCGGCCCGCGCCAATGGTCTTTTTTGATCCGGTTATCATGAAAATTGATTACCGTC
TGCTCTCGTGCCCATCTCCC

Downstream 100 bases:

>100_bases
AAAACCCGGCCGGGAAACCCCGGGCCGGGTTCGTTCGCCACGCCGGCCGCGTTGCGGCGGCCGCGCGACGTGCGGGATTA
CTTCGCGTTCGCGAATGCGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Als operon regulatory protein [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MELKQLRAFVTLAEELHFGRAAQRLCIVQPALSMQIKALEAELGARLFERDRHKVELSDTGRVFLPEARATLQQAARAEQ
LARLSSRGEIGTLRIAFVSSVLPTLLPAVLRTMRERYPLIVLELKDMPTPDQIAALRDRRIDFGMIRLPAAYAGIDTRVV
LEEGFVVALPLDHPLAAYDTIAPAALRDHPAFVLARRYAPGFHDDMLLALSRAGTTLEIAQEFGEFTTMLALVAAGMGIG
LIPAQAANALPPNVLARPLDLAGHRSGIGLAWTDLDSPLKRAFVDAVEHVMAHAGQ

Sequences:

>Translated_296_residues
MELKQLRAFVTLAEELHFGRAAQRLCIVQPALSMQIKALEAELGARLFERDRHKVELSDTGRVFLPEARATLQQAARAEQ
LARLSSRGEIGTLRIAFVSSVLPTLLPAVLRTMRERYPLIVLELKDMPTPDQIAALRDRRIDFGMIRLPAAYAGIDTRVV
LEEGFVVALPLDHPLAAYDTIAPAALRDHPAFVLARRYAPGFHDDMLLALSRAGTTLEIAQEFGEFTTMLALVAAGMGIG
LIPAQAANALPPNVLARPLDLAGHRSGIGLAWTDLDSPLKRAFVDAVEHVMAHAGQ
>Mature_296_residues
MELKQLRAFVTLAEELHFGRAAQRLCIVQPALSMQIKALEAELGARLFERDRHKVELSDTGRVFLPEARATLQQAARAEQ
LARLSSRGEIGTLRIAFVSSVLPTLLPAVLRTMRERYPLIVLELKDMPTPDQIAALRDRRIDFGMIRLPAAYAGIDTRVV
LEEGFVVALPLDHPLAAYDTIAPAALRDHPAFVLARRYAPGFHDDMLLALSRAGTTLEIAQEFGEFTTMLALVAAGMGIG
LIPAQAANALPPNVLARPLDLAGHRSGIGLAWTDLDSPLKRAFVDAVEHVMAHAGQ

Specific function: Regulates the expression of the alsSD operon for acetoin biosynthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=40.4, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1788887, Length=296, Percent_Identity=36.1486486486487, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI2367136, Length=265, Percent_Identity=35.4716981132075, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788748, Length=251, Percent_Identity=33.0677290836653, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI145693105, Length=245, Percent_Identity=30.2040816326531, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1790399, Length=297, Percent_Identity=27.9461279461279, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788297, Length=255, Percent_Identity=28.2352941176471, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI157672245, Length=245, Percent_Identity=26.9387755102041, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788481, Length=244, Percent_Identity=29.5081967213115, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081904, Length=210, Percent_Identity=27.6190476190476, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787530, Length=254, Percent_Identity=27.1653543307087, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787806, Length=263, Percent_Identity=26.2357414448669, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082132, Length=250, Percent_Identity=25.6, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787601, Length=179, Percent_Identity=27.9329608938547, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786401, Length=142, Percent_Identity=30.2816901408451, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32328; Mature: 32328

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKQLRAFVTLAEELHFGRAAQRLCIVQPALSMQIKALEAELGARLFERDRHKVELSDT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
GRVFLPEARATLQQAARAEQLARLSSRGEIGTLRIAFVSSVLPTLLPAVLRTMRERYPLI
CCEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VLELKDMPTPDQIAALRDRRIDFGMIRLPAAYAGIDTRVVLEEGFVVALPLDHPLAAYDT
EEEECCCCCHHHHHHHHHCCCCCCEEECCHHHHCCCEEEEEECCEEEEEECCCCHHHHHH
IAPAALRDHPAFVLARRYAPGFHDDMLLALSRAGTTLEIAQEFGEFTTMLALVAAGMGIG
HCCHHHHCCCHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
LIPAQAANALPPNVLARPLDLAGHRSGIGLAWTDLDSPLKRAFVDAVEHVMAHAGQ
CCCHHHCCCCCHHHHHCCHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MELKQLRAFVTLAEELHFGRAAQRLCIVQPALSMQIKALEAELGARLFERDRHKVELSDT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
GRVFLPEARATLQQAARAEQLARLSSRGEIGTLRIAFVSSVLPTLLPAVLRTMRERYPLI
CCEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VLELKDMPTPDQIAALRDRRIDFGMIRLPAAYAGIDTRVVLEEGFVVALPLDHPLAAYDT
EEEECCCCCHHHHHHHHHCCCCCCEEECCHHHHCCCEEEEEECCEEEEEECCCCHHHHHH
IAPAALRDHPAFVLARRYAPGFHDDMLLALSRAGTTLEIAQEFGEFTTMLALVAAGMGIG
HCCHHHHCCCHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
LIPAQAANALPPNVLARPLDLAGHRSGIGLAWTDLDSPLKRAFVDAVEHVMAHAGQ
CCCHHHCCCCCHHHHHCCHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377 [H]