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The map label for this gene is folD

Identifier: 103488573

GI number: 103488573

Start: 3246516

End: 3247424

Strand: Direct

Name: folD

Synonym: Sala_3097

Alternate gene names: 103488573

Gene position: 3246516-3247424 (Clockwise)

Preceding gene: 103488572

Following gene: 103488574

Centisome position: 97.05

GC content: 70.63

Gene sequence:

>909_bases
ATGACCGGTCCTGTTCCCGACGCCAGAGTGATCGATGGCAAGGCCTTTGCCGCGGGGCTGCGCGCGCGGATCGCCGACGC
GGTTCCCGCCTTCCGCGCGGCGACGGGCCGCGCGCCGGGGCTTGCCGTCGTGCTGGTCGGCGACGATCCGGCGAGCGCGG
TCTATGTCGGGTCAAAGGGCAAGGCGACCGTCGCGGCGGGCATGGCAAGCTTCGAGCACCGCCTGGCTGCGACGGCGACG
CAGGATGAGGTCGAGGCGCTGCTTCGCCAGCTCAACGCCGACGAGGCGGTCGACGGCATCCTGCTGCAATTGCCGCTTCC
CGGCCATCTCGACGAACAGGCGGCGGTCGCGACGATCGACCCCGACAAGGATGTCGACGGGCTGACCCCGGTCAGCGCGG
GCCGCCTTGCGCTCGGCATTCCCGGCATGGTGCCCTGCACTCCCTATGGCTGCCTGCTGCTTCTCCAGGACCGGCTCGGC
GATTTGTCGGGCAAGGATGCGATCGTCATCGGCCGCTCGATTCTGGTCGGCAAGCCGATGGGCCAATTGCTGCTCGGCGC
CAACTGCACGGTGACGATGGCGCACAGCCGGACCAAAGACCTGCCCGCGCTTGTGCGGCGCGCCGACATCGTCGTCGCCG
CGGTCGGCCGCGCCGAGATGGTGAAGGGCGACTGGATCAAGCCCGGCGCGATCGTCATCGACGTCGGCATCAACCGCCTC
CCGCCTGCGGACGGCGCGGCAAAGGGGCGACTGGTGGGCGATGTCGATTATGCCGCGGCGCTCGGCGTCGCCGATGCGAT
CACACCGGTGCCGGGCGGGGTCGGGCCGATGACCATTGCCTGCCTGCTCCGCAACACGCTCGTCGCCGCGCACCGCCGGG
CGGGGCTGGCCGATCCGGAAGGGTTCTGA

Upstream 100 bases:

>100_bases
TCTGACGCCGATGGTGGTGCTGATCCTGCTGATCATCCTGCAAGGGCCAGTGATGGCCTATCTCTACCGGCTCGGCATGC
AGACGGGGATGGCCTGACGC

Downstream 100 bases:

>100_bases
TGCGCCGGTTGCTCCTTTCAACAATTGCCATTTCCTTGCTGGCGGGCTGCGCGGGGCCGCGAGACGAGTTTCGCAACCGT
CCGCTCGCCGCCAACCCCAG

Product: methylenetetrahydrofolate dehydrogenase (NADP+)

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTGPVPDARVIDGKAFAAGLRARIADAVPAFRAATGRAPGLAVVLVGDDPASAVYVGSKGKATVAAGMASFEHRLAATAT
QDEVEALLRQLNADEAVDGILLQLPLPGHLDEQAAVATIDPDKDVDGLTPVSAGRLALGIPGMVPCTPYGCLLLLQDRLG
DLSGKDAIVIGRSILVGKPMGQLLLGANCTVTMAHSRTKDLPALVRRADIVVAAVGRAEMVKGDWIKPGAIVIDVGINRL
PPADGAAKGRLVGDVDYAAALGVADAITPVPGGVGPMTIACLLRNTLVAAHRRAGLADPEGF

Sequences:

>Translated_302_residues
MTGPVPDARVIDGKAFAAGLRARIADAVPAFRAATGRAPGLAVVLVGDDPASAVYVGSKGKATVAAGMASFEHRLAATAT
QDEVEALLRQLNADEAVDGILLQLPLPGHLDEQAAVATIDPDKDVDGLTPVSAGRLALGIPGMVPCTPYGCLLLLQDRLG
DLSGKDAIVIGRSILVGKPMGQLLLGANCTVTMAHSRTKDLPALVRRADIVVAAVGRAEMVKGDWIKPGAIVIDVGINRL
PPADGAAKGRLVGDVDYAAALGVADAITPVPGGVGPMTIACLLRNTLVAAHRRAGLADPEGF
>Mature_301_residues
TGPVPDARVIDGKAFAAGLRARIADAVPAFRAATGRAPGLAVVLVGDDPASAVYVGSKGKATVAAGMASFEHRLAATATQ
DEVEALLRQLNADEAVDGILLQLPLPGHLDEQAAVATIDPDKDVDGLTPVSAGRLALGIPGMVPCTPYGCLLLLQDRLGD
LSGKDAIVIGRSILVGKPMGQLLLGANCTVTMAHSRTKDLPALVRRADIVVAAVGRAEMVKGDWIKPGAIVIDVGINRLP
PADGAAKGRLVGDVDYAAALGVADAITPVPGGVGPMTIACLLRNTLVAAHRRAGLADPEGF

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=43.7931034482759, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI94721354, Length=305, Percent_Identity=40, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI222418558, Length=297, Percent_Identity=40.4040404040404, Blast_Score=191, Evalue=8e-49,
Organism=Escherichia coli, GI1786741, Length=283, Percent_Identity=49.8233215547703, Blast_Score=253, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17568735, Length=279, Percent_Identity=45.5197132616487, Blast_Score=215, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=43.7710437710438, Blast_Score=233, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=43.205574912892, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6322933, Length=308, Percent_Identity=24.6753246753247, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=45.6747404844291, Blast_Score=235, Evalue=3e-62,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=45.6747404844291, Blast_Score=235, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=45.6747404844291, Blast_Score=234, Evalue=5e-62,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=45.6747404844291, Blast_Score=234, Evalue=5e-62,
Organism=Drosophila melanogaster, GI17136816, Length=296, Percent_Identity=43.9189189189189, Blast_Score=225, Evalue=3e-59,
Organism=Drosophila melanogaster, GI17136818, Length=296, Percent_Identity=43.9189189189189, Blast_Score=225, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_SPHAL (Q1GNH1)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_618134.1
- ProteinModelPortal:   Q1GNH1
- SMR:   Q1GNH1
- GeneID:   4082833
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_3097
- NMPDR:   fig|317655.9.peg.2949
- HOGENOM:   HBG328751
- OMA:   LSCINAG
- BioCyc:   SALA317655:SALA_3097-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30722; Mature: 30591

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGPVPDARVIDGKAFAAGLRARIADAVPAFRAATGRAPGLAVVLVGDDPASAVYVGSKG
CCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCC
KATVAAGMASFEHRLAATATQDEVEALLRQLNADEAVDGILLQLPLPGHLDEQAAVATID
CEEHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCEEEEECCCCCCCCCCEEEEEC
PDKDVDGLTPVSAGRLALGIPGMVPCTPYGCLLLLQDRLGDLSGKDAIVIGRSILVGKPM
CCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECEEEECCCH
GQLLLGANCTVTMAHSRTKDLPALVRRADIVVAAVGRAEMVKGDWIKPGAIVIDVGINRL
HHHEECCCCEEEEECCCCCCHHHHHHHHCEEEEECCCHHHCCCCCCCCCEEEEECCCCCC
PPADGAAKGRLVGDVDYAAALGVADAITPVPGGVGPMTIACLLRNTLVAAHRRAGLADPE
CCCCCCCCCCEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GF
CC
>Mature Secondary Structure 
TGPVPDARVIDGKAFAAGLRARIADAVPAFRAATGRAPGLAVVLVGDDPASAVYVGSKG
CCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCC
KATVAAGMASFEHRLAATATQDEVEALLRQLNADEAVDGILLQLPLPGHLDEQAAVATID
CEEHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCEEEEECCCCCCCCCCEEEEEC
PDKDVDGLTPVSAGRLALGIPGMVPCTPYGCLLLLQDRLGDLSGKDAIVIGRSILVGKPM
CCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECEEEECCCH
GQLLLGANCTVTMAHSRTKDLPALVRRADIVVAAVGRAEMVKGDWIKPGAIVIDVGINRL
HHHEECCCCEEEEECCCCCCHHHHHHHHCEEEEECCCHHHCCCCCCCCCEEEEECCCCCC
PPADGAAKGRLVGDVDYAAALGVADAITPVPGGVGPMTIACLLRNTLVAAHRRAGLADPE
CCCCCCCCCCEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA