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The map label for this gene is rpoN2 [H]

Identifier: 103488476

GI number: 103488476

Start: 3141195

End: 3142649

Strand: Reverse

Name: rpoN2 [H]

Synonym: Sala_2999

Alternate gene names: 103488476

Gene position: 3142649-3141195 (Counterclockwise)

Preceding gene: 103488477

Following gene: 103488475

Centisome position: 93.95

GC content: 68.04

Gene sequence:

>1455_bases
ATGGCGTTGGGTCCGCGCCTCGATCTCCGCCAGTCGCAATCGCTGGTGATGACGCCGCAGTTGCAGCAGGCGATCAAGCT
GCTGGCGCTGTCGAACCTCGAGCTTGAGGCCTATCTGGCCGAGGCGTTGGAAGGCAATCCGCTGCTCGACACGGGCGCAC
CCGACAGCGAGGGCGGGGGCGACGAGCCCGACGGCGATGCGCCGCCCGCGGCGGAAACGCCTGCCGACACCGATCGGGCG
CTGGCGTCCGATGCCGGCACCGCCGACGACCTCGACGTCGATTTCACCGAAGAGCGTTTCCATCATGACAGCGCGAGCGA
CAGCGTCGGCCTGTCGGGTGCGGGCGAGGACGTGGATTTCGACAGTTTCGCGGAGGCCGAGGGCTCGCTCCACGACCATC
TGCTCGCGCAGGTCGGCGAGCGGCTCGACGGGATCGAGGCGATCATCGCGGGCCAGCTCGTCGCGCTGATCGACGAGGCG
GGCTATCTGCGCGCCGACCTTGCCGAACTCGCCGCACAGCTCGGCGTGCCCCTGGCGCTGGTCGAGGCGGTGCTGCGGGT
GGTCCAGGGCTTCGACCCCGCGGGCGTCGGCGCGCGCGACCTCGCCGAATGTATCGCCATCCAGGCGCGCGAGGCCGACC
GCTATGACCCCGCGATGGCGACGATGATCGCGCACCTCGATCTTGTCGCCAAGGGCGCCTTCCCGCAACTGAAGCGCATC
TGCGGCGTCGATGACGAGGATCTGGCCGACATGATCCGCGAACTGCGCGGCTATGATCCCAAACCGGGCTTCAGGTTCGG
CGGCTCGCCGGTGCAGGCGGTCGTTCCCGACCTGTATGTCCGGCGCACCGCGGCGGGCTGGGCGGTCGAGGTGAACAGCG
CGACCCTGCCGCGGCTGCTCGTCAACCGCCGCTATTACAACGAACTCGCCGCGGGCGCGGCGGCAAAAAGCAAGGCGTGG
CTGTCCGAACAGCTTGCCGGCGCCAACTGGCTGGTGCGCGCGCTCGACCAGCGCCAGCGCACGATCGTCAAGGTGGCGAG
CGAGATCGTCAAGCAGCAGGAGGGGTTTTTTCTGCACGGCGTCGCGCATATGCGGCCGCTGACCTTGCGCCAGGTCGCCG
AGGCGATCGGGATGCACGAATCGACCGTCAGCCGCGTCACCAGCAACAAATATCTGTCGTGCCCACGCGGCCTGTTCGAG
CTCAAATATTTCTTCTCGTCGGGCATATCGGCGACCGAGGGCGACGGCGCGGTGTCGGCCGAGGCGGTCAAGAGCCGGAT
CAAGGCGTTGATCGAGGGCGAGGACGCGCGCGCGATCCTGTCCGACGAGACGATCGCGCAGAAACTGTCGGCCGAAGGAT
TCGACATCGCGCGGCGCACCGTCGTCAAATATCGCGAGGCGATGGGTTATGGCTCGTCGGTGCAGCGACGGCGGCAGAAA
GCGCTGGCGGGATAG

Upstream 100 bases:

>100_bases
CGTTCGTGTCGAGCGAAGTCGAGACACGCCGAGTTCGTGCAAGCCTTCACGTGTCTCGACTTCGCTCGACACGAACGGGA
TCGTAATCAGGTAATTTCCG

Downstream 100 bases:

>100_bases
GCACGCCTCCAGTTCCGTTCGTGTCGAGCGAAGTCGAGACACCGATCGGGACGGCGCAAGATCGAGGGGCATCTCGCCTT
CGCTCGATGCGAACGGATAA

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MALGPRLDLRQSQSLVMTPQLQQAIKLLALSNLELEAYLAEALEGNPLLDTGAPDSEGGGDEPDGDAPPAAETPADTDRA
LASDAGTADDLDVDFTEERFHHDSASDSVGLSGAGEDVDFDSFAEAEGSLHDHLLAQVGERLDGIEAIIAGQLVALIDEA
GYLRADLAELAAQLGVPLALVEAVLRVVQGFDPAGVGARDLAECIAIQAREADRYDPAMATMIAHLDLVAKGAFPQLKRI
CGVDDEDLADMIRELRGYDPKPGFRFGGSPVQAVVPDLYVRRTAAGWAVEVNSATLPRLLVNRRYYNELAAGAAAKSKAW
LSEQLAGANWLVRALDQRQRTIVKVASEIVKQQEGFFLHGVAHMRPLTLRQVAEAIGMHESTVSRVTSNKYLSCPRGLFE
LKYFFSSGISATEGDGAVSAEAVKSRIKALIEGEDARAILSDETIAQKLSAEGFDIARRTVVKYREAMGYGSSVQRRRQK
ALAG

Sequences:

>Translated_484_residues
MALGPRLDLRQSQSLVMTPQLQQAIKLLALSNLELEAYLAEALEGNPLLDTGAPDSEGGGDEPDGDAPPAAETPADTDRA
LASDAGTADDLDVDFTEERFHHDSASDSVGLSGAGEDVDFDSFAEAEGSLHDHLLAQVGERLDGIEAIIAGQLVALIDEA
GYLRADLAELAAQLGVPLALVEAVLRVVQGFDPAGVGARDLAECIAIQAREADRYDPAMATMIAHLDLVAKGAFPQLKRI
CGVDDEDLADMIRELRGYDPKPGFRFGGSPVQAVVPDLYVRRTAAGWAVEVNSATLPRLLVNRRYYNELAAGAAAKSKAW
LSEQLAGANWLVRALDQRQRTIVKVASEIVKQQEGFFLHGVAHMRPLTLRQVAEAIGMHESTVSRVTSNKYLSCPRGLFE
LKYFFSSGISATEGDGAVSAEAVKSRIKALIEGEDARAILSDETIAQKLSAEGFDIARRTVVKYREAMGYGSSVQRRRQK
ALAG
>Mature_483_residues
ALGPRLDLRQSQSLVMTPQLQQAIKLLALSNLELEAYLAEALEGNPLLDTGAPDSEGGGDEPDGDAPPAAETPADTDRAL
ASDAGTADDLDVDFTEERFHHDSASDSVGLSGAGEDVDFDSFAEAEGSLHDHLLAQVGERLDGIEAIIAGQLVALIDEAG
YLRADLAELAAQLGVPLALVEAVLRVVQGFDPAGVGARDLAECIAIQAREADRYDPAMATMIAHLDLVAKGAFPQLKRIC
GVDDEDLADMIRELRGYDPKPGFRFGGSPVQAVVPDLYVRRTAAGWAVEVNSATLPRLLVNRRYYNELAAGAAAKSKAWL
SEQLAGANWLVRALDQRQRTIVKVASEIVKQQEGFFLHGVAHMRPLTLRQVAEAIGMHESTVSRVTSNKYLSCPRGLFEL
KYFFSSGISATEGDGAVSAEAVKSRIKALIEGEDARAILSDETIAQKLSAEGFDIARRTVVKYREAMGYGSSVQRRRQKA
LAG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=488, Percent_Identity=37.2950819672131, Blast_Score=302, Evalue=4e-83,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 51893; Mature: 51762

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00717 SIGMA54_1 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALGPRLDLRQSQSLVMTPQLQQAIKLLALSNLELEAYLAEALEGNPLLDTGAPDSEGGG
CCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
DEPDGDAPPAAETPADTDRALASDAGTADDLDVDFTEERFHHDSASDSVGLSGAGEDVDF
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCC
DSFAEAEGSLHDHLLAQVGERLDGIEAIIAGQLVALIDEAGYLRADLAELAAQLGVPLAL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHH
VEAVLRVVQGFDPAGVGARDLAECIAIQAREADRYDPAMATMIAHLDLVAKGAFPQLKRI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
CGVDDEDLADMIRELRGYDPKPGFRFGGSPVQAVVPDLYVRRTAAGWAVEVNSATLPRLL
HCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHH
VNRRYYNELAAGAAAKSKAWLSEQLAGANWLVRALDQRQRTIVKVASEIVKQQEGFFLHG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEC
VAHMRPLTLRQVAEAIGMHESTVSRVTSNKYLSCPRGLFELKYFFSSGISATEGDGAVSA
HHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCH
EAVKSRIKALIEGEDARAILSDETIAQKLSAEGFDIARRTVVKYREAMGYGSSVQRRRQK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
ALAG
HCCC
>Mature Secondary Structure 
ALGPRLDLRQSQSLVMTPQLQQAIKLLALSNLELEAYLAEALEGNPLLDTGAPDSEGGG
CCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
DEPDGDAPPAAETPADTDRALASDAGTADDLDVDFTEERFHHDSASDSVGLSGAGEDVDF
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCC
DSFAEAEGSLHDHLLAQVGERLDGIEAIIAGQLVALIDEAGYLRADLAELAAQLGVPLAL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHH
VEAVLRVVQGFDPAGVGARDLAECIAIQAREADRYDPAMATMIAHLDLVAKGAFPQLKRI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
CGVDDEDLADMIRELRGYDPKPGFRFGGSPVQAVVPDLYVRRTAAGWAVEVNSATLPRLL
HCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHH
VNRRYYNELAAGAAAKSKAWLSEQLAGANWLVRALDQRQRTIVKVASEIVKQQEGFFLHG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEC
VAHMRPLTLRQVAEAIGMHESTVSRVTSNKYLSCPRGLFELKYFFSSGISATEGDGAVSA
HHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCH
EAVKSRIKALIEGEDARAILSDETIAQKLSAEGFDIARRTVVKYREAMGYGSSVQRRRQK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
ALAG
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1991712; 12597275 [H]