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The map label for this gene is nemA [H]

Identifier: 103488461

GI number: 103488461

Start: 3126615

End: 3127700

Strand: Reverse

Name: nemA [H]

Synonym: Sala_2984

Alternate gene names: 103488461

Gene position: 3127700-3126615 (Counterclockwise)

Preceding gene: 103488467

Following gene: 103488460

Centisome position: 93.5

GC content: 67.68

Gene sequence:

>1086_bases
ATGACCGCATCGCTGTTCCAGCCGATCACGCTGGGCGCCATCGAAGCGCCGAACCGCATCATCATGGCGCCGCTGACGCG
CGGCCGCGCGGGACCGGGCTTCGTTCCCAATGAGCTCGCGCGCGAATATTATCGCCAGCGCGCCTCGGCGGGGCTGATCA
TTTCGGAAGCGACGGGGATTTCGCAGGAGGGGCTCGGCTGGCCGAATGCGCCGGGCCTGTGGACCGATGCGCAGGTCGAA
GGCTGGAAGCCGGTGACCGAAGCCGTCCATGAGGCGGGGGGCCGCATCGTTGCCCAGCTCTGGCATATGGGCCGCGTCGT
CCATTCGGTGTTCAACGACGGCAAACCACCCGTCTCGGCTTCGGCGACGCGGGCGCCAGGCAAGGCGCACACCCCGGTCG
GCCGCCTCGAATATGAAGTTGCGCGCCCGCTCGAACCGGGCGAAATCCCGCGCGTCATCGCCGATTATGCGAAGGCCGCC
GAAAATGCGAAGCGCGCCGGTTTCGACGGCGTCCAGCTGCACGGCGCAAACGGTTATCTGATCGACCAGTTCCTGCGCGA
CAATTCAAATCTGCGCGACGATGATTATGGCGGCCCGGTCGAAAACCGCATCCGCCTGCTCCGCGAAGTGACCGAAGCGC
TGATCTCGGTCTGGGGCGCCGACCGCGTCGGTGTGCGTCTCTCGCCCAATGGCGATACGCAGGGCGTCGACGACAGCGCG
CCCGAAAGGCTGTTCCCGATCGCCGCGGCGGCGCTCGACGCGCTCGGCATCGCCTTCCTCGAGTTGCGCGAGCCCGGCCC
CGACGGCACATTCGGCAGAACGGATGTGCCCAAGCAGTCGCCGTCGATCCGCGCCGCGTTCAGGGGCCCGCTGATCCTCA
ACAGCGACTACACCGCCGCGCTCGCCGACGAAGCGATCGCCAGCGGCATCGCCGACGCGATCGCCTTCGGGCGGCCCTTC
ATCGCCAATCCCGACCTCGTCGAGCGCATCCGCACCGGCGCCGAATGGGCCGCCGACAATCCGCAGACCTGGTATTCGCC
GGGGCCCGAAGGTTACATCGATTATCCGGTACTGGAGGCGGCCTGA

Upstream 100 bases:

>100_bases
CACTTACCCATTGTGCCGACGCGGCGTGACCCATAGCTTCGTCATCGAGCGCGGCGACCAAGGGGGCCGTCGGCGCAGCA
TTTGACGAGATGGAGGAAAT

Downstream 100 bases:

>100_bases
TCGGAGACGGGTCGTCCCCGCACAGGCGGGGGCGGCCCGCCCTATCCCTTTTCCGCCAACGCCTTGTCGATGATGCTCGC
ACCGATCGGCGCCAATATCC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTASLFQPITLGAIEAPNRIIMAPLTRGRAGPGFVPNELAREYYRQRASAGLIISEATGISQEGLGWPNAPGLWTDAQVE
GWKPVTEAVHEAGGRIVAQLWHMGRVVHSVFNDGKPPVSASATRAPGKAHTPVGRLEYEVARPLEPGEIPRVIADYAKAA
ENAKRAGFDGVQLHGANGYLIDQFLRDNSNLRDDDYGGPVENRIRLLREVTEALISVWGADRVGVRLSPNGDTQGVDDSA
PERLFPIAAAALDALGIAFLELREPGPDGTFGRTDVPKQSPSIRAAFRGPLILNSDYTAALADEAIASGIADAIAFGRPF
IANPDLVERIRTGAEWAADNPQTWYSPGPEGYIDYPVLEAA

Sequences:

>Translated_361_residues
MTASLFQPITLGAIEAPNRIIMAPLTRGRAGPGFVPNELAREYYRQRASAGLIISEATGISQEGLGWPNAPGLWTDAQVE
GWKPVTEAVHEAGGRIVAQLWHMGRVVHSVFNDGKPPVSASATRAPGKAHTPVGRLEYEVARPLEPGEIPRVIADYAKAA
ENAKRAGFDGVQLHGANGYLIDQFLRDNSNLRDDDYGGPVENRIRLLREVTEALISVWGADRVGVRLSPNGDTQGVDDSA
PERLFPIAAAALDALGIAFLELREPGPDGTFGRTDVPKQSPSIRAAFRGPLILNSDYTAALADEAIASGIADAIAFGRPF
IANPDLVERIRTGAEWAADNPQTWYSPGPEGYIDYPVLEAA
>Mature_360_residues
TASLFQPITLGAIEAPNRIIMAPLTRGRAGPGFVPNELAREYYRQRASAGLIISEATGISQEGLGWPNAPGLWTDAQVEG
WKPVTEAVHEAGGRIVAQLWHMGRVVHSVFNDGKPPVSASATRAPGKAHTPVGRLEYEVARPLEPGEIPRVIADYAKAAE
NAKRAGFDGVQLHGANGYLIDQFLRDNSNLRDDDYGGPVENRIRLLREVTEALISVWGADRVGVRLSPNGDTQGVDDSAP
ERLFPIAAAALDALGIAFLELREPGPDGTFGRTDVPKQSPSIRAAFRGPLILNSDYTAALADEAIASGIADAIAFGRPFI
ANPDLVERIRTGAEWAADNPQTWYSPGPEGYIDYPVLEAA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=367, Percent_Identity=45.5040871934605, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI1789463, Length=344, Percent_Identity=29.3604651162791, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17565138, Length=353, Percent_Identity=29.7450424929178, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=271, Percent_Identity=29.8892988929889, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17559804, Length=271, Percent_Identity=28.7822878228782, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI72001454, Length=271, Percent_Identity=29.1512915129151, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17564188, Length=262, Percent_Identity=27.0992366412214, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17540738, Length=273, Percent_Identity=27.8388278388278, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=380, Percent_Identity=36.3157894736842, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6321973, Length=380, Percent_Identity=35.5263157894737, Blast_Score=196, Evalue=5e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38701; Mature: 38570

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASLFQPITLGAIEAPNRIIMAPLTRGRAGPGFVPNELAREYYRQRASAGLIISEATGI
CCCCCCCCHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCC
SQEGLGWPNAPGLWTDAQVEGWKPVTEAVHEAGGRIVAQLWHMGRVVHSVFNDGKPPVSA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
SATRAPGKAHTPVGRLEYEVARPLEPGEIPRVIADYAKAAENAKRAGFDGVQLHGANGYL
CCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHH
IDQFLRDNSNLRDDDYGGPVENRIRLLREVTEALISVWGADRVGVRLSPNGDTQGVDDSA
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCC
PERLFPIAAAALDALGIAFLELREPGPDGTFGRTDVPKQSPSIRAAFRGPLILNSDYTAA
CHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCHHH
LADEAIASGIADAIAFGRPFIANPDLVERIRTGAEWAADNPQTWYSPGPEGYIDYPVLEA
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCC
A
C
>Mature Secondary Structure 
TASLFQPITLGAIEAPNRIIMAPLTRGRAGPGFVPNELAREYYRQRASAGLIISEATGI
CCCCCCCHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCC
SQEGLGWPNAPGLWTDAQVEGWKPVTEAVHEAGGRIVAQLWHMGRVVHSVFNDGKPPVSA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
SATRAPGKAHTPVGRLEYEVARPLEPGEIPRVIADYAKAAENAKRAGFDGVQLHGANGYL
CCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHH
IDQFLRDNSNLRDDDYGGPVENRIRLLREVTEALISVWGADRVGVRLSPNGDTQGVDDSA
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCC
PERLFPIAAAALDALGIAFLELREPGPDGTFGRTDVPKQSPSIRAAFRGPLILNSDYTAA
CHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCHHH
LADEAIASGIADAIAFGRPFIANPDLVERIRTGAEWAADNPQTWYSPGPEGYIDYPVLEA
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]