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The map label for this gene is dapF

Identifier: 103488207

GI number: 103488207

Start: 2876673

End: 2877479

Strand: Direct

Name: dapF

Synonym: Sala_2730

Alternate gene names: 103488207

Gene position: 2876673-2877479 (Clockwise)

Preceding gene: 103488206

Following gene: 103488208

Centisome position: 85.99

GC content: 68.15

Gene sequence:

>807_bases
ATGGCAGATCGCTTCACCAAGATGCATGGGCTTGGCAACGACTTCGTCGTGATCGACGCGCGCGTGGCGCCGGTCGAGAT
GACGCCGGCGCGCGCGCATGCGATCGCCGACCGGCGCCACGGCATCGGGTGCGATCAGCTGATCCTGCTCGAACCCTCGA
CCAGCGCGGACGTGAAGATGCGCATCTTCAACGCCGATGGGGGCGAGGTCGAGGCGTGCGGCAATGCGACGCGCTGTGTC
GCGACGCTGATCGGCAAGCCCGCGGTGATCGAGACGCTGGCGGGGATGCTGCGCGTCACCCCCGCCGACGGCGGCGCCGA
GGTCACGCTGGGTGAACCCGTCTTCGACTGGGACCATATCCCGCTCGCGATGCCGATGGACACGCGCGACATGCCTGTCG
CGTGGGACGAGCTGGAACATGGCGCCGCGGTCAATGTCGGTAATCCGCACATCGTCTTTTTCGTGCCGGAAGCCGATGCG
GTTGCGCTCGATCAACTGGGTCCGCGGATCGAGACCGACCCGCTGTTCCCCGAGCGCGTCAATGTCAACGTCGCGAGCCT
CGACGGCGAGAATCAGCTGCAATTGCGCGTCTGGGAACGCGGTGTGGGGCTCACGCAGGCGTGCGGCACCGGCGCCTGCG
CCACTGCGGTCGCCGCGATCCGTGCCGGACTGGTGCGGTCGCCGGTGACCGTCGCGCTGCCCGGCGGTGATCTGGTCATC
CGCTGGGCACCCGGCGAACCCATCGTGATGAGCGGCGCCGCGACACGCGTGTTCGACGGCGAAACCGACTGGGCACAGTT
CGGATGA

Upstream 100 bases:

>100_bases
CCGACGCAGGGCGCCGACGCGGAAGGGTTGCTGCGCAAGACCGCTTGATTCCGGCGCCTCGCCTAGACTTCGCGCGTCCG
CGCCCTTACATGCGCGGACT

Downstream 100 bases:

>100_bases
ACGCAGAACTCGCCGACCGCGTGGAGGTCGTCAATTTTGGTTGCCGGCTCAACATCGCCGAGGGCGAGGCGATTCGTGCG
GCCGTGAAGGCCGCGGGCGC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MADRFTKMHGLGNDFVVIDARVAPVEMTPARAHAIADRRHGIGCDQLILLEPSTSADVKMRIFNADGGEVEACGNATRCV
ATLIGKPAVIETLAGMLRVTPADGGAEVTLGEPVFDWDHIPLAMPMDTRDMPVAWDELEHGAAVNVGNPHIVFFVPEADA
VALDQLGPRIETDPLFPERVNVNVASLDGENQLQLRVWERGVGLTQACGTGACATAVAAIRAGLVRSPVTVALPGGDLVI
RWAPGEPIVMSGAATRVFDGETDWAQFG

Sequences:

>Translated_268_residues
MADRFTKMHGLGNDFVVIDARVAPVEMTPARAHAIADRRHGIGCDQLILLEPSTSADVKMRIFNADGGEVEACGNATRCV
ATLIGKPAVIETLAGMLRVTPADGGAEVTLGEPVFDWDHIPLAMPMDTRDMPVAWDELEHGAAVNVGNPHIVFFVPEADA
VALDQLGPRIETDPLFPERVNVNVASLDGENQLQLRVWERGVGLTQACGTGACATAVAAIRAGLVRSPVTVALPGGDLVI
RWAPGEPIVMSGAATRVFDGETDWAQFG
>Mature_267_residues
ADRFTKMHGLGNDFVVIDARVAPVEMTPARAHAIADRRHGIGCDQLILLEPSTSADVKMRIFNADGGEVEACGNATRCVA
TLIGKPAVIETLAGMLRVTPADGGAEVTLGEPVFDWDHIPLAMPMDTRDMPVAWDELEHGAAVNVGNPHIVFFVPEADAV
ALDQLGPRIETDPLFPERVNVNVASLDGENQLQLRVWERGVGLTQACGTGACATAVAAIRAGLVRSPVTVALPGGDLVIR
WAPGEPIVMSGAATRVFDGETDWAQFG

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=269, Percent_Identity=40.1486988847584, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_SPHAL (Q1GPI7)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617768.1
- ProteinModelPortal:   Q1GPI7
- SMR:   Q1GPI7
- GeneID:   4082469
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2730
- NMPDR:   fig|317655.9.peg.2596
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   SALA317655:SALA_2730-MONOMER
- BRENDA:   5.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 28392; Mature: 28261

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 73-73 ACT_SITE 208-208

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRFTKMHGLGNDFVVIDARVAPVEMTPARAHAIADRRHGIGCDQLILLEPSTSADVKM
CCCCHHHHHCCCCCEEEEEEEECCEEECCHHHHHHHHHHCCCCCCEEEEECCCCCCCEEE
RIFNADGGEVEACGNATRCVATLIGKPAVIETLAGMLRVTPADGGAEVTLGEPVFDWDHI
EEEECCCCCEEECCCHHHHHHHHHCCCHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCC
PLAMPMDTRDMPVAWDELEHGAAVNVGNPHIVFFVPEADAVALDQLGPRIETDPLFPERV
CEECCCCCCCCCCCHHHHCCCCEEECCCCEEEEEECCCCEEEHHHCCCCCCCCCCCCCCE
NVNVASLDGENQLQLRVWERGVGLTQACGTGACATAVAAIRAGLVRSPVTVALPGGDLVI
EEEEEEECCCCEEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEE
RWAPGEPIVMSGAATRVFDGETDWAQFG
EECCCCCEEECCCEEEEECCCCCHHCCC
>Mature Secondary Structure 
ADRFTKMHGLGNDFVVIDARVAPVEMTPARAHAIADRRHGIGCDQLILLEPSTSADVKM
CCCHHHHHCCCCCEEEEEEEECCEEECCHHHHHHHHHHCCCCCCEEEEECCCCCCCEEE
RIFNADGGEVEACGNATRCVATLIGKPAVIETLAGMLRVTPADGGAEVTLGEPVFDWDHI
EEEECCCCCEEECCCHHHHHHHHHCCCHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCC
PLAMPMDTRDMPVAWDELEHGAAVNVGNPHIVFFVPEADAVALDQLGPRIETDPLFPERV
CEECCCCCCCCCCCHHHHCCCCEEECCCCEEEEEECCCCEEEHHHCCCCCCCCCCCCCCE
NVNVASLDGENQLQLRVWERGVGLTQACGTGACATAVAAIRAGLVRSPVTVALPGGDLVI
EEEEEEECCCCEEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEE
RWAPGEPIVMSGAATRVFDGETDWAQFG
EECCCCCEEECCCEEEEECCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA