Click here to switch to the map view.

The map label for this gene is ycjG [H]

Identifier: 103487459

GI number: 103487459

Start: 2083542

End: 2084531

Strand: Reverse

Name: ycjG [H]

Synonym: Sala_1976

Alternate gene names: 103487459

Gene position: 2084531-2083542 (Counterclockwise)

Preceding gene: 103487467

Following gene: 103487457

Centisome position: 62.31

GC content: 69.6

Gene sequence:

>990_bases
ATGGCTATCCAACTGAAAAGCGCGCGCGTCGAACGCTGGCCCGTCGCGGGCTCGTTCGTCATCAGTCGCGGCGCCAGGAA
TCATGTCGATGTCGTTGTCGCGGAGATCGAGGACAGCGGCGCGATCGGGCGCGGCGAGGGGACGCCGGTTTATTATCTGG
GCGAGGATGCGGCCACGTGCCGCGACCAGATCCTGCTCGCCGCGGCGCACGTCGCCGACATGGCCGCGGCCGAGGCGCGC
GCGGCGGTGCAGGAGATCATGGCGCCGGGAGCGGCGCGCAATGCGCTCGATTGCGCGCTCTGGGATCTGGAGGCGCGACA
GCGTGGCCTGAGGCTCTGGCAGCTTGCGGGCTGCGACGGCGCGCCGACCGCACGGACGACCGCCTTCACCATCTCGCTCG
GCAGTCCGGGCGCGATGGCCGAACAGGCGGCGACGGCGGCGGCCGACGGCTATCATCTGCTCAAGATCAAGCTGACGGGC
GAAGGCGACCGGCTGCGCGTCGCCGGCGTGCGCAAGGGCGCTCCCCACGCCCGGCTGATCGTCGACGCCAATGAAAGCTG
GGCGCAGGTCGACATATTGGAAGAGGCCGAGGCACTGGCCGACATGGGCGTTGAAATGATCGAACAGCCGGTGCCGGTCG
GCGCCGACGCGCTGCTCGATCCCGTTTATGCGTCGATTCCCTTCGTCGCCGACGAAAGCTGCCAGACGGCGGCCGACGTT
GCGCGCATCGGCGCCTTTTACGACGGGGTGAACATCAAGCTCGACAAGGCGGGCGGGCTGACCGAGGCGCTGCGCATCGC
CGACGCGGCCGACGCTGCGGGGCTGGCGATCATGGTCGGCTGCATGCTCTCGACCAGCCTTGCGATCGCGCCCGCTTTCG
TGCTCGCCCAGCGCGCGCGCTGGGTCGATTTGGACGGCCCGGCTTTGCTCGAACGCGACCGCGAGGGCGGGTTCGAATTT
CGCGAAGGCCGCATCGGCACGCCGCGCTAG

Upstream 100 bases:

>100_bases
GCCAGGCGGTGTGCGCCTCGACCGCGAGGCGGCGGCCAAGGTCGCCGATGCGTTCGGAAAGGGATTTGCCGGTCATGCCA
TCCCTCATTACTGTCTAGCC

Downstream 100 bases:

>100_bases
GGTCCAAACTCAATCAGCCTCTTGAAAATCGGCGCGCACATTGATTCACGGGTTCTCCGCTGAAGCAGGAGGCCTTTGGA
AATGTCGCGTTCGTTGTTCT

Product: mandelate racemase/muconate lactonizing enzyme

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MAIQLKSARVERWPVAGSFVISRGARNHVDVVVAEIEDSGAIGRGEGTPVYYLGEDAATCRDQILLAAAHVADMAAAEAR
AAVQEIMAPGAARNALDCALWDLEARQRGLRLWQLAGCDGAPTARTTAFTISLGSPGAMAEQAATAAADGYHLLKIKLTG
EGDRLRVAGVRKGAPHARLIVDANESWAQVDILEEAEALADMGVEMIEQPVPVGADALLDPVYASIPFVADESCQTAADV
ARIGAFYDGVNIKLDKAGGLTEALRIADAADAAGLAIMVGCMLSTSLAIAPAFVLAQRARWVDLDGPALLERDREGGFEF
REGRIGTPR

Sequences:

>Translated_329_residues
MAIQLKSARVERWPVAGSFVISRGARNHVDVVVAEIEDSGAIGRGEGTPVYYLGEDAATCRDQILLAAAHVADMAAAEAR
AAVQEIMAPGAARNALDCALWDLEARQRGLRLWQLAGCDGAPTARTTAFTISLGSPGAMAEQAATAAADGYHLLKIKLTG
EGDRLRVAGVRKGAPHARLIVDANESWAQVDILEEAEALADMGVEMIEQPVPVGADALLDPVYASIPFVADESCQTAADV
ARIGAFYDGVNIKLDKAGGLTEALRIADAADAAGLAIMVGCMLSTSLAIAPAFVLAQRARWVDLDGPALLERDREGGFEF
REGRIGTPR
>Mature_328_residues
AIQLKSARVERWPVAGSFVISRGARNHVDVVVAEIEDSGAIGRGEGTPVYYLGEDAATCRDQILLAAAHVADMAAAEARA
AVQEIMAPGAARNALDCALWDLEARQRGLRLWQLAGCDGAPTARTTAFTISLGSPGAMAEQAATAAADGYHLLKIKLTGE
GDRLRVAGVRKGAPHARLIVDANESWAQVDILEEAEALADMGVEMIEQPVPVGADALLDPVYASIPFVADESCQTAADVA
RIGAFYDGVNIKLDKAGGLTEALRIADAADAAGLAIMVGCMLSTSLAIAPAFVLAQRARWVDLDGPALLERDREGGFEFR
EGRIGTPR

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=315, Percent_Identity=43.4920634920635, Blast_Score=225, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 34557; Mature: 34425

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIQLKSARVERWPVAGSFVISRGARNHVDVVVAEIEDSGAIGRGEGTPVYYLGEDAATC
CEEEECCCCCCCCCCCCHHEEECCCCCCEEEEEEEECCCCCCCCCCCCEEEEECCCHHHH
RDQILLAAAHVADMAAAEARAAVQEIMAPGAARNALDCALWDLEARQRGLRLWQLAGCDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHCCCEEEEEECCCC
APTARTTAFTISLGSPGAMAEQAATAAADGYHLLKIKLTGEGDRLRVAGVRKGAPHARLI
CCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEEEECCCCEEEEEEECCCCCCEEEE
VDANESWAQVDILEEAEALADMGVEMIEQPVPVGADALLDPVYASIPFVADESCQTAADV
EECCCCCEEEHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHCCCEECCCHHHHHHH
ARIGAFYDGVNIKLDKAGGLTEALRIADAADAAGLAIMVGCMLSTSLAIAPAFVLAQRAR
HHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
WVDLDGPALLERDREGGFEFREGRIGTPR
EEECCCHHHHHCCCCCCCEECCCCCCCCC
>Mature Secondary Structure 
AIQLKSARVERWPVAGSFVISRGARNHVDVVVAEIEDSGAIGRGEGTPVYYLGEDAATC
EEEECCCCCCCCCCCCHHEEECCCCCCEEEEEEEECCCCCCCCCCCCEEEEECCCHHHH
RDQILLAAAHVADMAAAEARAAVQEIMAPGAARNALDCALWDLEARQRGLRLWQLAGCDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHCCCEEEEEECCCC
APTARTTAFTISLGSPGAMAEQAATAAADGYHLLKIKLTGEGDRLRVAGVRKGAPHARLI
CCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEEEECCCCEEEEEEECCCCCCEEEE
VDANESWAQVDILEEAEALADMGVEMIEQPVPVGADALLDPVYASIPFVADESCQTAADV
EECCCCCEEEHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHCCCEECCCHHHHHHH
ARIGAFYDGVNIKLDKAGGLTEALRIADAADAAGLAIMVGCMLSTSLAIAPAFVLAQRAR
HHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
WVDLDGPALLERDREGGFEFREGRIGTPR
EEECCCHHHHHCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]