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The map label for this gene is purU [H]

Identifier: 103487321

GI number: 103487321

Start: 1931717

End: 1932589

Strand: Reverse

Name: purU [H]

Synonym: Sala_1837

Alternate gene names: 103487321

Gene position: 1932589-1931717 (Counterclockwise)

Preceding gene: 103487322

Following gene: 103487320

Centisome position: 57.77

GC content: 65.75

Gene sequence:

>873_bases
ATGACCGGGGGGAACGGGCCCGCTTATGTGCTGACCTTCAGCTGTGCCGATGCGGTGGGCATCGTCGCCGCGGTGACGGG
GCTGCTCGCGGACCGCGACGGCTTCATTCTCGACAGCCAGCAATATGCCGACCTCGATTCGGGCCGCTTTTTCATGCGCG
TTGAGTTTCGCGGCGAAGGGTCGCGCTTTCCGGGCAACCTCGCCGGGGTGCAGGACGCCTTTGCGCCCATCGCCCGTCGT
TTTCAGATGGACGCAAGGATCAGCGACCGCGCCGCCAAACCGCGCTTCGTCATCGCGGTGTCGCAGGGCAGCCACTGTCT
GAACGACCTGCTCCACCGCTGGTCGACGGGCAATCTTGCCATCGACATCGTCGGCGTGGTGTCGAACCATGAACATCTGC
GCCGCCTGACCGAATGGCATGGCGTGCCCTTTCATTACCTGCCGGTGAGCGATGCGAACCGCGCCGAACAGGAGTCGGCG
ATCCTCGATGTCATGGCGCGCGGCGGCGCCGAATATCTGGTGCTCGCCCGCTACATGCAGGTGTTGTCGGAAGATCTTTC
GGCGCGGCTTGCGGGCCGCTGCATCAATATCCATCACAGCTTCCTGCCGGGTTTCAAGGGCGCGCGGCCCTATCACCGCG
CGCACGAGCGCGGGGTCAAGCTGATCGGCGCGACGGCGCATTTCGTGACCAGCGACCTCGACGAGGGGCCGATCATCGAA
CAGGCGGTCGAGCGCGTCGATCATCGCGACGGCGTGGACGACCTGATCCGCATCGGCCGCGATGTCGAGGCGCAGGTGCT
CGCGCGAGCGGTGCGCTGGGTCGCCGAACAGCGCGTGCTGATCGACGGACGCAAGACCGTCGTGTTTCGCTAG

Upstream 100 bases:

>100_bases
GGCATCCGCCATGCGACCGAAGGGCGGGTCGTCTATCTCGCCTATCTGCGCGATCCGGCGGGCAACAAGCTTTGCGCCAC
CCACAAGATTTCGGGCTGAC

Downstream 100 bases:

>100_bases
GGTCAGCAGGAAGCCCTCAAAGGCCGCTCGCGTCCCGACTTCGCTCGACACCGGCGGGAAGGTAGGGCAGCGTAGGTAGC
GAATCGCAACCAGAATATCA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MTGGNGPAYVLTFSCADAVGIVAAVTGLLADRDGFILDSQQYADLDSGRFFMRVEFRGEGSRFPGNLAGVQDAFAPIARR
FQMDARISDRAAKPRFVIAVSQGSHCLNDLLHRWSTGNLAIDIVGVVSNHEHLRRLTEWHGVPFHYLPVSDANRAEQESA
ILDVMARGGAEYLVLARYMQVLSEDLSARLAGRCINIHHSFLPGFKGARPYHRAHERGVKLIGATAHFVTSDLDEGPIIE
QAVERVDHRDGVDDLIRIGRDVEAQVLARAVRWVAEQRVLIDGRKTVVFR

Sequences:

>Translated_290_residues
MTGGNGPAYVLTFSCADAVGIVAAVTGLLADRDGFILDSQQYADLDSGRFFMRVEFRGEGSRFPGNLAGVQDAFAPIARR
FQMDARISDRAAKPRFVIAVSQGSHCLNDLLHRWSTGNLAIDIVGVVSNHEHLRRLTEWHGVPFHYLPVSDANRAEQESA
ILDVMARGGAEYLVLARYMQVLSEDLSARLAGRCINIHHSFLPGFKGARPYHRAHERGVKLIGATAHFVTSDLDEGPIIE
QAVERVDHRDGVDDLIRIGRDVEAQVLARAVRWVAEQRVLIDGRKTVVFR
>Mature_289_residues
TGGNGPAYVLTFSCADAVGIVAAVTGLLADRDGFILDSQQYADLDSGRFFMRVEFRGEGSRFPGNLAGVQDAFAPIARRF
QMDARISDRAAKPRFVIAVSQGSHCLNDLLHRWSTGNLAIDIVGVVSNHEHLRRLTEWHGVPFHYLPVSDANRAEQESAI
LDVMARGGAEYLVLARYMQVLSEDLSARLAGRCINIHHSFLPGFKGARPYHRAHERGVKLIGATAHFVTSDLDEGPIIEQ
AVERVDHRDGVDDLIRIGRDVEAQVLARAVRWVAEQRVLIDGRKTVVFR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=31.5789473684211, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=31.5789473684211, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=31.5789473684211, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1787483, Length=280, Percent_Identity=41.4285714285714, Blast_Score=217, Evalue=7e-58,
Organism=Escherichia coli, GI1788846, Length=109, Percent_Identity=35.7798165137615, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=177, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32079; Mature: 31948

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGGNGPAYVLTFSCADAVGIVAAVTGLLADRDGFILDSQQYADLDSGRFFMRVEFRGEG
CCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCEECCHHHCCCCCCEEEEEEEEECCC
SRFPGNLAGVQDAFAPIARRFQMDARISDRAAKPRFVIAVSQGSHCLNDLLHRWSTGNLA
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCCEE
IDIVGVVSNHEHLRRLTEWHGVPFHYLPVSDANRAEQESAILDVMARGGAEYLVLARYMQ
EEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
VLSEDLSARLAGRCINIHHSFLPGFKGARPYHRAHERGVKLIGATAHFVTSDLDEGPIIE
HHHHHHHHHHHHHHHEEHHHHCCCCCCCCCHHHHHHCCCEEEEHHHHHHHCCCCCCCHHH
QAVERVDHRDGVDDLIRIGRDVEAQVLARAVRWVAEQRVLIDGRKTVVFR
HHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCEEEEEC
>Mature Secondary Structure 
TGGNGPAYVLTFSCADAVGIVAAVTGLLADRDGFILDSQQYADLDSGRFFMRVEFRGEG
CCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCEECCHHHCCCCCCEEEEEEEEECCC
SRFPGNLAGVQDAFAPIARRFQMDARISDRAAKPRFVIAVSQGSHCLNDLLHRWSTGNLA
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCCEE
IDIVGVVSNHEHLRRLTEWHGVPFHYLPVSDANRAEQESAILDVMARGGAEYLVLARYMQ
EEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
VLSEDLSARLAGRCINIHHSFLPGFKGARPYHRAHERGVKLIGATAHFVTSDLDEGPIIE
HHHHHHHHHHHHHHHEEHHHHCCCCCCCCCHHHHHHCCCEEEEHHHHHHHCCCCCCCHHH
QAVERVDHRDGVDDLIRIGRDVEAQVLARAVRWVAEQRVLIDGRKTVVFR
HHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]