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The map label for this gene is dosC [H]

Identifier: 103487180

GI number: 103487180

Start: 1785381

End: 1787165

Strand: Reverse

Name: dosC [H]

Synonym: Sala_1695

Alternate gene names: 103487180

Gene position: 1787165-1785381 (Counterclockwise)

Preceding gene: 103487181

Following gene: 103487172

Centisome position: 53.43

GC content: 65.94

Gene sequence:

>1785_bases
ATGATCCGGTCGCTGTCCCCCAGAGTCGAAGCGGTCTTTTGGTTCCTGTTGACCGTCACAGGCTATTATCTGCTCGCGAG
CCTGTCGCTCTATGCGACGCGGGGCGCCGACAATATCGCTGCGGTTTGGCCGGCAAGCGGATATATCCTGGCGTTGTTGC
TGCTCATGCCCGCCCGGGGGCGCGTGCCAGCCTTTGCCGGCATGGCCGTCGCCAGCATCGCGGCCAACATGGGCGCGACG
ACGCCATTTTGGACGAGCCTCTCCTTCACTTTCGCGAACATGGTCGAGGCGATCATCGCGTTATGGCTGATCCGGCGCCA
TGAGCCCGGCGAACTGTCGTTCATGGCGCCGCGCGCCGTCATCCATTTTTGCGTCGCGGCGGTGGCCGCCAGTCTGGTCG
GCGCGGGGCTGGCGACGCTGCTGGCCGGTCGCGGCGGCGATTTCTTCCTGTCGTGGGCGACGACGGTGTTGCTCGGCATG
TTGATCGTGACGCCGCCGATCGTGATGCTGACACGGATGATGGAGTTGAAGGCATTCGGCAAGGTGCCGCGCGCGATGCT
GGTCGAGGCGACGGCGATACTGACCGGCGCCGGTGCCGTCACGATCCTCGTCTTTTCGCAGTCACATTTTCCCGCGACCT
TCCTGCCGTCCATCGCGGTGGTCGCGGCCGCCTATCGGCTCGGGCCTTTCGGCGCGGCGGCGGGGATGCTGATCGTCACG
ATCATCGCATCGCTCCTGTCGGGGCAGGGTTATGGGCCGATGGCGGCGATCGACAGCGATCCGAAGGTCAAGGTGTTGTT
CCTGCAATTCTATCTGCTGTCGCTGCTGCTGGCGGCGTTGCCGCTCGCCGCATTGCTGATCGGTCGCCAGCGGCTCGCCA
GGCGTCTCGAGCAAAGCAACCGCTGGCTGCTCCAGGCCGAAGCTGCGGCGCTCGTCGGCCACTGGCGCGTCAATCTGGTC
GACTGGACGATCTATTGGTCCGACCAGACCTATCGCATACACGGGCTGGAACCCGGAACGCCCGTCGACGTCGATTACAG
CGTCCGTCAATATCTGGCCGAAGATCGCGCCGCAGTGCAACGGGTGCTTGAAGAGGCAGTGCGGTCGGGCGAACCCTTTG
AGTTCCATGGCCGTATCCTGCGCGCTGACGGGCAGGTGCGCCACGTCAAGTCGCACGGGTCTATCGAAAGGGGCCGCGGC
GGGGGCGCGACGGGTATCTTCGGCACAGTGCAGGATGTAACCGAAACCGTCGAGAATGCCCGCATCCTGGAGGCCGCACG
GAGCGCCGCCGAACGTGTCGCCAACACCGACATGCTCACCGGCCTGCCCAATCGGCGCCATACGCTGACCTTTCTCGAAG
AGGCGATGGCCGGCGCGCGCGCGCATGGCGCGCCGTTGGCGGTGGCGATCTTTGACATCGACCATTTCAAGCGGATCAAC
GACACGCACGGCCATGCGACCGGCGACCAGGTGATCCAGCGCGTCGCACAGCGCGCCAGATCGGTGCTGCGCGACGAGGA
CATGATCGGCCGCATCGGCGGCGAGGAGTTTGTCTGCATCCTTCAGCGCGCGAGCGCGCTGTCGGGCGAGATCGTCGCCG
AGCGCGTGCGCAAGGCGGTCGAGAACGGGGTCGGCCAGGGCGACGGGCTGCCGCCGACGACCGTCAGCATCGGGCTGGCG
GTCTATGACGGCGAACAGGATATCGAGGAACTGCTGCACCGTGCCGACCAGGCACTTTATGCGGCGAAACGCGAGGGGCG
CAACCGGATGCGGCGCGCGGCGTAG

Upstream 100 bases:

>100_bases
GCCGAAGTCGTCGGCCTGACCTTTGGCGGCGTGGGGTGAAGCGTGGCGCGGGCGCCGAAATCTTAGCGGTTTATTTTCCT
GTTTCGGACTATCGGGTTGC

Downstream 100 bases:

>100_bases
CCATACCCCGCAGGCATACGCCGCATTCGGTTCGTGAAAAAGGACGCGGAAGCTGTGCTCCCGCGTCCTTTTTCGATCAA
CTTGCCCTGAACAGGCTCAG

Product: diguanylate cyclase with PAS/PAC sensor

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MIRSLSPRVEAVFWFLLTVTGYYLLASLSLYATRGADNIAAVWPASGYILALLLLMPARGRVPAFAGMAVASIAANMGAT
TPFWTSLSFTFANMVEAIIALWLIRRHEPGELSFMAPRAVIHFCVAAVAASLVGAGLATLLAGRGGDFFLSWATTVLLGM
LIVTPPIVMLTRMMELKAFGKVPRAMLVEATAILTGAGAVTILVFSQSHFPATFLPSIAVVAAAYRLGPFGAAAGMLIVT
IIASLLSGQGYGPMAAIDSDPKVKVLFLQFYLLSLLLAALPLAALLIGRQRLARRLEQSNRWLLQAEAAALVGHWRVNLV
DWTIYWSDQTYRIHGLEPGTPVDVDYSVRQYLAEDRAAVQRVLEEAVRSGEPFEFHGRILRADGQVRHVKSHGSIERGRG
GGATGIFGTVQDVTETVENARILEAARSAAERVANTDMLTGLPNRRHTLTFLEEAMAGARAHGAPLAVAIFDIDHFKRIN
DTHGHATGDQVIQRVAQRARSVLRDEDMIGRIGGEEFVCILQRASALSGEIVAERVRKAVENGVGQGDGLPPTTVSIGLA
VYDGEQDIEELLHRADQALYAAKREGRNRMRRAA

Sequences:

>Translated_594_residues
MIRSLSPRVEAVFWFLLTVTGYYLLASLSLYATRGADNIAAVWPASGYILALLLLMPARGRVPAFAGMAVASIAANMGAT
TPFWTSLSFTFANMVEAIIALWLIRRHEPGELSFMAPRAVIHFCVAAVAASLVGAGLATLLAGRGGDFFLSWATTVLLGM
LIVTPPIVMLTRMMELKAFGKVPRAMLVEATAILTGAGAVTILVFSQSHFPATFLPSIAVVAAAYRLGPFGAAAGMLIVT
IIASLLSGQGYGPMAAIDSDPKVKVLFLQFYLLSLLLAALPLAALLIGRQRLARRLEQSNRWLLQAEAAALVGHWRVNLV
DWTIYWSDQTYRIHGLEPGTPVDVDYSVRQYLAEDRAAVQRVLEEAVRSGEPFEFHGRILRADGQVRHVKSHGSIERGRG
GGATGIFGTVQDVTETVENARILEAARSAAERVANTDMLTGLPNRRHTLTFLEEAMAGARAHGAPLAVAIFDIDHFKRIN
DTHGHATGDQVIQRVAQRARSVLRDEDMIGRIGGEEFVCILQRASALSGEIVAERVRKAVENGVGQGDGLPPTTVSIGLA
VYDGEQDIEELLHRADQALYAAKREGRNRMRRAA
>Mature_594_residues
MIRSLSPRVEAVFWFLLTVTGYYLLASLSLYATRGADNIAAVWPASGYILALLLLMPARGRVPAFAGMAVASIAANMGAT
TPFWTSLSFTFANMVEAIIALWLIRRHEPGELSFMAPRAVIHFCVAAVAASLVGAGLATLLAGRGGDFFLSWATTVLLGM
LIVTPPIVMLTRMMELKAFGKVPRAMLVEATAILTGAGAVTILVFSQSHFPATFLPSIAVVAAAYRLGPFGAAAGMLIVT
IIASLLSGQGYGPMAAIDSDPKVKVLFLQFYLLSLLLAALPLAALLIGRQRLARRLEQSNRWLLQAEAAALVGHWRVNLV
DWTIYWSDQTYRIHGLEPGTPVDVDYSVRQYLAEDRAAVQRVLEEAVRSGEPFEFHGRILRADGQVRHVKSHGSIERGRG
GGATGIFGTVQDVTETVENARILEAARSAAERVANTDMLTGLPNRRHTLTFLEEAMAGARAHGAPLAVAIFDIDHFKRIN
DTHGHATGDQVIQRVAQRARSVLRDEDMIGRIGGEEFVCILQRASALSGEIVAERVRKAVENGVGQGDGLPPTTVSIGLA
VYDGEQDIEELLHRADQALYAAKREGRNRMRRAA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=42.6751592356688, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87081881, Length=221, Percent_Identity=37.10407239819, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1787262, Length=175, Percent_Identity=37.7142857142857, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786584, Length=192, Percent_Identity=35.4166666666667, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1787802, Length=170, Percent_Identity=32.3529411764706, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082007, Length=178, Percent_Identity=36.5168539325843, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=35.2201257861635, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=32.1212121212121, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787541, Length=225, Percent_Identity=29.7777777777778, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI87081977, Length=154, Percent_Identity=33.1168831168831, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788381, Length=219, Percent_Identity=29.6803652968037, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87081921, Length=242, Percent_Identity=25.2066115702479, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=158, Percent_Identity=28.4810126582279, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788956, Length=179, Percent_Identity=30.1675977653631, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 64208; Mature: 64208

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRSLSPRVEAVFWFLLTVTGYYLLASLSLYATRGADNIAAVWPASGYILALLLLMPARG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCC
RVPAFAGMAVASIAANMGATTPFWTSLSFTFANMVEAIIALWLIRRHEPGELSFMAPRAV
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHH
IHFCVAAVAASLVGAGLATLLAGRGGDFFLSWATTVLLGMLIVTPPIVMLTRMMELKAFG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KVPRAMLVEATAILTGAGAVTILVFSQSHFPATFLPSIAVVAAAYRLGPFGAAAGMLIVT
CCCHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
IIASLLSGQGYGPMAAIDSDPKVKVLFLQFYLLSLLLAALPLAALLIGRQRLARRLEQSN
HHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RWLLQAEAAALVGHWRVNLVDWTIYWSDQTYRIHGLEPGTPVDVDYSVRQYLAEDRAAVQ
CEEEEEHHHHHHHHEEEEEEEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
RVLEEAVRSGEPFEFHGRILRADGQVRHVKSHGSIERGRGGGATGIFGTVQDVTETVENA
HHHHHHHHCCCCEEECCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RILEAARSAAERVANTDMLTGLPNRRHTLTFLEEAMAGARAHGAPLAVAIFDIDHFKRIN
HHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHCC
DTHGHATGDQVIQRVAQRARSVLRDEDMIGRIGGEEFVCILQRASALSGEIVAERVRKAV
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
ENGVGQGDGLPPTTVSIGLAVYDGEQDIEELLHRADQALYAAKREGRNRMRRAA
HCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRSLSPRVEAVFWFLLTVTGYYLLASLSLYATRGADNIAAVWPASGYILALLLLMPARG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCC
RVPAFAGMAVASIAANMGATTPFWTSLSFTFANMVEAIIALWLIRRHEPGELSFMAPRAV
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHH
IHFCVAAVAASLVGAGLATLLAGRGGDFFLSWATTVLLGMLIVTPPIVMLTRMMELKAFG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KVPRAMLVEATAILTGAGAVTILVFSQSHFPATFLPSIAVVAAAYRLGPFGAAAGMLIVT
CCCHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
IIASLLSGQGYGPMAAIDSDPKVKVLFLQFYLLSLLLAALPLAALLIGRQRLARRLEQSN
HHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RWLLQAEAAALVGHWRVNLVDWTIYWSDQTYRIHGLEPGTPVDVDYSVRQYLAEDRAAVQ
CEEEEEHHHHHHHHEEEEEEEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
RVLEEAVRSGEPFEFHGRILRADGQVRHVKSHGSIERGRGGGATGIFGTVQDVTETVENA
HHHHHHHHCCCCEEECCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RILEAARSAAERVANTDMLTGLPNRRHTLTFLEEAMAGARAHGAPLAVAIFDIDHFKRIN
HHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHCC
DTHGHATGDQVIQRVAQRARSVLRDEDMIGRIGGEEFVCILQRASALSGEIVAERVRKAV
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
ENGVGQGDGLPPTTVSIGLAVYDGEQDIEELLHRADQALYAAKREGRNRMRRAA
HCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]