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The map label for this gene is glyS [H]

Identifier: 103487096

GI number: 103487096

Start: 1691545

End: 1693716

Strand: Reverse

Name: glyS [H]

Synonym: Sala_1611

Alternate gene names: 103487096

Gene position: 1693716-1691545 (Counterclockwise)

Preceding gene: 103487097

Following gene: 103487095

Centisome position: 50.63

GC content: 64.96

Gene sequence:

>2172_bases
ATGACCGATTTTCTTCTCGAACTGCGCAGCGAGGAAATCCCCGCGCGGATGCAGGCCGGCGCGCGCGCCGAGCTAGAAAA
GCTGTTCCGGACGCAGATGAGCGCCGCGGGTATCGTGGTCGGCGATCTGACCATCTGGTCGACGCCGCGCCGCCTTGCCC
TGATCGCTCGCGACCTGCCCGACGCGACCGCAGCCGTCAGCGAGGAACTCAAAGGCCCGCGCACCAGCGCGCCGCCGCAG
GCCCTCGAAGGCTTCCTGCGCAAGACCGGGCTGACGCAGGACCAGCTCGAAGACCGCGACGGCGTCTGGTTCGCCGTGAT
CGACAAGCCGGGCCGCGCGACCGCCGAGGTGCTGGCCGAGGCGATCCCCGCGATCGTCCGCGCTTTTGCCTGGCCCAAGT
CGATGCGCTGGGGCAGGGACAGTGCGAGCAGCGAAAGCCTGCGCTGGGTGCGCCCGCTGTCGGGCATCGTCGCGATCTTT
GGCGAAGCGCTGATTCCATGTGAAGTTGGGGGGATTTCCGCCGGTTTCGCAACGCGCGGCCACCGTTTTCACTGCCCGGG
CGAGATCACGATCGGCTCGGCATCGGACTATGCGGAAAAGCTGCGCGCATGCCATGTCATCGTCGACCATGCGGAGCGGG
CGCGTATCATCCGCGACGGCGCCGCCAAGGCCGCGGCCGACGCCGGGCTGAGCCTCGTCGAGGACGAGGGGCTGGTGATC
GAGAATGCCGGCCTCACCGAATGGCCGGTGCCGCTTCTGGGGCGCTTCGACGAGGCGTTCCTGGCGGTGCCGCCGGAGGT
GATCCAGCTGACCGCGCGGGTGAACCAGAAATATTTTGTCGTGAACGATGCGGATGGGAAGCTGGCGAACGGTTTTGTCT
GCACCGCGAATATCGATGCGGTCGACGGCGGCGCGGAGATTGTGGCGGGGAACCAGAAGGTGCTGGCGGCGCGATTGTCG
GATGCGCGCTTCTTCTGGGAACAGGATCGCAAGACGAAGCTGGCGGATCACGCGAAGAAGCTGGACCGGATCACCTTTCA
CGAAAAGCTGGGGACGGTCGCGGACAAGGTCGAGCGCGTTGCGAAGCTGGCCGAATGGCTGGCGAACGAAGGCATCGTCC
CGAATTGCGACCCAGCGCTCGCGCGGCAGGCGGCCGAGCTGGCGAAAGCCGACCTCGTCACCGAAATGGTCGGCGAGTTC
CCCGAATTGCAGGGCCTGATGGGCGGCTATTACGCCCGCGCCGAAGGGCTTCCGCAAGCGGTCGCCGACGCGATCCGCGA
CCATTACAAGCCGGTCGGGCAGGGCGATGACGTCCCGACCGCGCCGGTGACGGTGGCTGTGGCGTTGGCGGATAAGCTGG
ATACGATTGCTGGCTTCTTCATGAAGAATATGAAGCCGACCGGGTCGAAAGACCCCTTTGCCTTGCGCCGCGCTGGATTG
GGTATCATTGCGCTAACGGTCAGAAGCTCGTTACGCGGAGATATTGGGACTTGGCTGGCTGCAGCACGTCGATACTTTGT
CGATCAGGACATCGTAGATGAGCGGGAATATCCTGATGCCTACGCAACGAAAGATGCTGCAATTTCCGCAGGCCTTGCCA
CAGTTTTCATGGATGAAGTTCGCGACTTCATCGCAGACCGCCTCAAAGTCCAGCAGCGCGAAGCCGGCGTCCGGCACGAC
CTGATCGACGCGGTTTTCGCGCTCGGCGGCGAGGATGATCTGGTTCGCTTGCTTGCGCGGGTGAAGGCGTTGCAGGCGTT
TATGGCGACCGAGGACGGCACCAACCTGCTCGCGGGTTACAAGCGTGCAGCGAACATTTTGAAACAAGCCAGTGTTCCGG
CGGAAGCCGGAACCCGGGGCGGGGCAGCGCAGGTGTTGGAGGAAGGCACTCCTGGGCTCCGGCTTTCGCCGGAGTGCATG
ACGGTGCATGACGCCGCGCTCCTCGCAGCCCTCGACACCGCCGAACCGGCTGCGCTCAACGCCGTCGCCGACGAACGCTT
CACCGACGCCATGGCCGCCCTCGCCAGCCTTCGCGCGCCGATCGACGCCTTTTTCGAGGGCGTGATGGTCAACGACCCCG
ACGAAGTGGTGCGCGCCTATCGGCTTGGACTGCTCGCGCGCTTTACCGGCGCGGTGCATGGCGTTGCGGATTTCTCGAAG
ATAGAGGGCTGA

Upstream 100 bases:

>100_bases
GTGTCGAGCGAAGTCGAGACACGCAGCGGCCTAGCGCTGTCCTTCGCGGCATCTCGACTTCGCTCGATGCGAACGGGGTT
TGGGGATCGGGGGATTTGGC

Downstream 100 bases:

>100_bases
GACCACATTTGAAATCCCGTTCGTGTCGAGCGAAGTCGAGACACCGGGAAGGCGAGCAGGAACGATGGGCATCTCGACTT
CGCTCGATGCGAACGGAGTA

Product: glycine--tRNA ligase

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 723; Mature: 722

Protein sequence:

>723_residues
MTDFLLELRSEEIPARMQAGARAELEKLFRTQMSAAGIVVGDLTIWSTPRRLALIARDLPDATAAVSEELKGPRTSAPPQ
ALEGFLRKTGLTQDQLEDRDGVWFAVIDKPGRATAEVLAEAIPAIVRAFAWPKSMRWGRDSASSESLRWVRPLSGIVAIF
GEALIPCEVGGISAGFATRGHRFHCPGEITIGSASDYAEKLRACHVIVDHAERARIIRDGAAKAAADAGLSLVEDEGLVI
ENAGLTEWPVPLLGRFDEAFLAVPPEVIQLTARVNQKYFVVNDADGKLANGFVCTANIDAVDGGAEIVAGNQKVLAARLS
DARFFWEQDRKTKLADHAKKLDRITFHEKLGTVADKVERVAKLAEWLANEGIVPNCDPALARQAAELAKADLVTEMVGEF
PELQGLMGGYYARAEGLPQAVADAIRDHYKPVGQGDDVPTAPVTVAVALADKLDTIAGFFMKNMKPTGSKDPFALRRAGL
GIIALTVRSSLRGDIGTWLAAARRYFVDQDIVDEREYPDAYATKDAAISAGLATVFMDEVRDFIADRLKVQQREAGVRHD
LIDAVFALGGEDDLVRLLARVKALQAFMATEDGTNLLAGYKRAANILKQASVPAEAGTRGGAAQVLEEGTPGLRLSPECM
TVHDAALLAALDTAEPAALNAVADERFTDAMAALASLRAPIDAFFEGVMVNDPDEVVRAYRLGLLARFTGAVHGVADFSK
IEG

Sequences:

>Translated_723_residues
MTDFLLELRSEEIPARMQAGARAELEKLFRTQMSAAGIVVGDLTIWSTPRRLALIARDLPDATAAVSEELKGPRTSAPPQ
ALEGFLRKTGLTQDQLEDRDGVWFAVIDKPGRATAEVLAEAIPAIVRAFAWPKSMRWGRDSASSESLRWVRPLSGIVAIF
GEALIPCEVGGISAGFATRGHRFHCPGEITIGSASDYAEKLRACHVIVDHAERARIIRDGAAKAAADAGLSLVEDEGLVI
ENAGLTEWPVPLLGRFDEAFLAVPPEVIQLTARVNQKYFVVNDADGKLANGFVCTANIDAVDGGAEIVAGNQKVLAARLS
DARFFWEQDRKTKLADHAKKLDRITFHEKLGTVADKVERVAKLAEWLANEGIVPNCDPALARQAAELAKADLVTEMVGEF
PELQGLMGGYYARAEGLPQAVADAIRDHYKPVGQGDDVPTAPVTVAVALADKLDTIAGFFMKNMKPTGSKDPFALRRAGL
GIIALTVRSSLRGDIGTWLAAARRYFVDQDIVDEREYPDAYATKDAAISAGLATVFMDEVRDFIADRLKVQQREAGVRHD
LIDAVFALGGEDDLVRLLARVKALQAFMATEDGTNLLAGYKRAANILKQASVPAEAGTRGGAAQVLEEGTPGLRLSPECM
TVHDAALLAALDTAEPAALNAVADERFTDAMAALASLRAPIDAFFEGVMVNDPDEVVRAYRLGLLARFTGAVHGVADFSK
IEG
>Mature_722_residues
TDFLLELRSEEIPARMQAGARAELEKLFRTQMSAAGIVVGDLTIWSTPRRLALIARDLPDATAAVSEELKGPRTSAPPQA
LEGFLRKTGLTQDQLEDRDGVWFAVIDKPGRATAEVLAEAIPAIVRAFAWPKSMRWGRDSASSESLRWVRPLSGIVAIFG
EALIPCEVGGISAGFATRGHRFHCPGEITIGSASDYAEKLRACHVIVDHAERARIIRDGAAKAAADAGLSLVEDEGLVIE
NAGLTEWPVPLLGRFDEAFLAVPPEVIQLTARVNQKYFVVNDADGKLANGFVCTANIDAVDGGAEIVAGNQKVLAARLSD
ARFFWEQDRKTKLADHAKKLDRITFHEKLGTVADKVERVAKLAEWLANEGIVPNCDPALARQAAELAKADLVTEMVGEFP
ELQGLMGGYYARAEGLPQAVADAIRDHYKPVGQGDDVPTAPVTVAVALADKLDTIAGFFMKNMKPTGSKDPFALRRAGLG
IIALTVRSSLRGDIGTWLAAARRYFVDQDIVDEREYPDAYATKDAAISAGLATVFMDEVRDFIADRLKVQQREAGVRHDL
IDAVFALGGEDDLVRLLARVKALQAFMATEDGTNLLAGYKRAANILKQASVPAEAGTRGGAAQVLEEGTPGLRLSPECMT
VHDAALLAALDTAEPAALNAVADERFTDAMAALASLRAPIDAFFEGVMVNDPDEVVRAYRLGLLARFTGAVHGVADFSKI
EG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=731, Percent_Identity=34.3365253077975, Blast_Score=355, Evalue=5e-99,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 77695; Mature: 77564

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFLLELRSEEIPARMQAGARAELEKLFRTQMSAAGIVVGDLTIWSTPRRLALIARDLP
CCHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHCCEEEEEEEEECCCHHHHHHHHCCC
DATAAVSEELKGPRTSAPPQALEGFLRKTGLTQDQLEDRDGVWFAVIDKPGRATAEVLAE
HHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCEEEEECCCCCHHHHHHHH
AIPAIVRAFAWPKSMRWGRDSASSESLRWVRPLSGIVAIFGEALIPCEVGGISAGFATRG
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHCCEECCCCCCCCCCCC
HRFHCPGEITIGSASDYAEKLRACHVIVDHAERARIIRDGAAKAAADAGLSLVEDEGLVI
CEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEECCCEEE
ENAGLTEWPVPLLGRFDEAFLAVPPEVIQLTARVNQKYFVVNDADGKLANGFVCTANIDA
ECCCCCCCCCHHHCCCCHHHHCCCHHHHHHHHHCCCEEEEEECCCCCEECCEEEEECCCC
VDGGAEIVAGNQKVLAARLSDARFFWEQDRKTKLADHAKKLDRITFHEKLGTVADKVERV
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKLAEWLANEGIVPNCDPALARQAAELAKADLVTEMVGEFPELQGLMGGYYARAEGLPQA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHH
VADAIRDHYKPVGQGDDVPTAPVTVAVALADKLDTIAGFFMKNMKPTGSKDPFALRRAGL
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCC
GIIALTVRSSLRGDIGTWLAAARRYFVDQDIVDEREYPDAYATKDAAISAGLATVFMDEV
CEEEEEHHHHHCCHHHHHHHHHHHHHCCHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHH
RDFIADRLKVQQREAGVRHDLIDAVFALGGEDDLVRLLARVKALQAFMATEDGTNLLAGY
HHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KRAANILKQASVPAEAGTRGGAAQVLEEGTPGLRLSPECMTVHDAALLAALDTAEPAALN
HHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCEECCHHHHHHHHHHHHHHCCCCCHHHH
AVADERFTDAMAALASLRAPIDAFFEGVMVNDPDEVVRAYRLGLLARFTGAVHGVADFSK
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEG
CCC
>Mature Secondary Structure 
TDFLLELRSEEIPARMQAGARAELEKLFRTQMSAAGIVVGDLTIWSTPRRLALIARDLP
CHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHCCEEEEEEEEECCCHHHHHHHHCCC
DATAAVSEELKGPRTSAPPQALEGFLRKTGLTQDQLEDRDGVWFAVIDKPGRATAEVLAE
HHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCEEEEECCCCCHHHHHHHH
AIPAIVRAFAWPKSMRWGRDSASSESLRWVRPLSGIVAIFGEALIPCEVGGISAGFATRG
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHCCEECCCCCCCCCCCC
HRFHCPGEITIGSASDYAEKLRACHVIVDHAERARIIRDGAAKAAADAGLSLVEDEGLVI
CEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEECCCEEE
ENAGLTEWPVPLLGRFDEAFLAVPPEVIQLTARVNQKYFVVNDADGKLANGFVCTANIDA
ECCCCCCCCCHHHCCCCHHHHCCCHHHHHHHHHCCCEEEEEECCCCCEECCEEEEECCCC
VDGGAEIVAGNQKVLAARLSDARFFWEQDRKTKLADHAKKLDRITFHEKLGTVADKVERV
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKLAEWLANEGIVPNCDPALARQAAELAKADLVTEMVGEFPELQGLMGGYYARAEGLPQA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHH
VADAIRDHYKPVGQGDDVPTAPVTVAVALADKLDTIAGFFMKNMKPTGSKDPFALRRAGL
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCC
GIIALTVRSSLRGDIGTWLAAARRYFVDQDIVDEREYPDAYATKDAAISAGLATVFMDEV
CEEEEEHHHHHCCHHHHHHHHHHHHHCCHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHH
RDFIADRLKVQQREAGVRHDLIDAVFALGGEDDLVRLLARVKALQAFMATEDGTNLLAGY
HHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KRAANILKQASVPAEAGTRGGAAQVLEEGTPGLRLSPECMTVHDAALLAALDTAEPAALN
HHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCEECCHHHHHHHHHHHHHHCCCCCHHHH
AVADERFTDAMAALASLRAPIDAFFEGVMVNDPDEVVRAYRLGLLARFTGAVHGVADFSK
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA