| Definition | Sphingopyxis alaskensis RB2256, complete genome. |
|---|---|
| Accession | NC_008048 |
| Length | 3,345,170 |
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The map label for this gene is ppdK [H]
Identifier: 103487095
GI number: 103487095
Start: 1688690
End: 1691347
Strand: Reverse
Name: ppdK [H]
Synonym: Sala_1610
Alternate gene names: 103487095
Gene position: 1691347-1688690 (Counterclockwise)
Preceding gene: 103487096
Following gene: 103487094
Centisome position: 50.56
GC content: 66.82
Gene sequence:
>2658_bases ATGACGAAGATGGTGCATTTGTTCGGAGGTGCGGTCACCACGGCCGAGCGTTCGAAGGAGTTGCTGGGCGGCAAGGGGTC GAATCTTGCCGAAATGGCCTCGATCGGCCTGCCGGTCCCGCCGGGCTTCACGATCACCACCGACGTCTGCACCGCCTATT ACGCCAATGGCGAGCAATTTCCGGCCGGGCTGGTCGAAGAGGTCGCCGCGGGCATCGCGCATATCGAGGGGATTACCGGC AAGCGTTTCGGCGACGCCGTCGACCCGTTGCTCGTCTCGGTCCGTTCGGGCGCGCGCGTGTCGATGCCGGGGATGATGGA CACCGTCCTCAACCTCGGGCTCAACGACAAGACCGTCGTCGGGCTCGCCGAAGCATCGGGCGACCCGCGCTTCGCGTGGG ACAGCTATCGCCGCTTCGTCCAGATGTACGCCGACGTCGTCATGGGCCTCGACCATGCCGAGTTCGAGGAAGCGCTGGAA ATCGCCAAGGAAGACAAGGGCTTTTATCTCGACACCGAGATGGCGGCAGAGGACTGGCAGGCGCTGGTCAAGCAATATCA GGCGATCGTCGAACGCGAAACCGGCGCGCCTTTCCCGCAGGAACCGAAGGACCAGCTGTGGGGCGCGGTCGGCGCGGTGT TCGCGAGCTGGGAAAGCGACCGTGCGAAAGTCTATCGCCGCCTCAATTCGATCCCCGCCGAATGGGGCACCGCGGTCAAT GTGCAGGCGATGGTGTTCGGCAATATGGGCGATACGTCGGCGACGGGCGTGGCGTTCACGCGCGATCCCGCGACCGGCGA GCGCGCCTGGTACGGCGAATGGCTGATCAATGCCCAGGGTGAAGACGTCGTCGCGGGCATCCGCACGCCGCAATATCTGA CGAAGGCCGCGCGTGAGAGGGCGGGCGCCAGGCCGCTGTCGATGGAAGAGGCGATGCCCGAAACCTTCGCCGAGCTGGGC CGCGTCTTCGACACGCTCGAAACCCATTATCGCGACATGCAGGACATCGAGTTCACCGTCGAACGCGGCAAGCTGTGGAT GCTGCAAACCCGTTCGGGCAAGCGCACCGCCAAGGCGGCGCTGAAGATCGCGGTCGATATGGCGGCCGAGGGATTGATTA CCGAAGAGGAAGCCGTCGGCCGCGTCGATCCCGGCGCGCTCGACCAACTGCTCCACCCGACACTCGACCCGAAAGCGCCG CGCGACGTGCTGACCAAGGGGCTCCCCGCCAGCCCCGGCGCGGCGTCGGGCAAGATCATGTTCGACGCCGACAGTGCGGA GAAAGCCGCGGCGATGGGCGAAGCGGTGATCCTCGTCCGCGTCGAGACGAGCCCCGAGGACATTCACGGGATGCACGCGG CAAAAGGCATTTTGACCGCGCGCGGCGGGATGACCAGCCACGCGGCGGTCGTCGCGCGCGGCATGGGGCGTCCGTGCGTT TCGGGCGCGGGCGGGCTCAGCATCGACGGCGCCGCGCGCGTGCTGCGCGTCGGCGGGCGTGAGCTGCGCGAGGGCGATAT TTTGACCCTCGACGGGTCGACCGGCGAAGTGATGGCCGGCGAGGTGCCGACGCTTATGCCCGAGCTGGTCGGCGATTTCG GCACGCTGATGGGCTGGGCCGACAAGGTGCGCCGGATGAAGGTGCGCACCAATGCGGAGACGCCGCAGGACGCCAGGGTC GCGCGCGACTTCGGCGCCGAGGGCATCGGCCTCTGCCGCACCGAACATATGTTCTTCGACGCCGCGCGCATCACCGCGGT GCGCGAGATGATCCTTGCCGACAGCGAGGAGGGCCGCCGTGCGGCGCTCGCCAAGCTGCTCCCCGAACAGCGCGGCGATT TTGCTGCGATCTTCGGCGTGATGGCGGGGCTGCCGGTGACGATCCGCCTGCTCGATCCGCCGCTCCACGAGTTCCTGCCG ACGCGCGAGGAGGATTTCGCCGACGTCGCCGCAGCCGCGGGGGTGGGGATCGAGGCGCTCAAGGCGCGTGCCAACGAATT GCACGAGTTCAACCCGATGCTCGGCCATCGCGGCTGCCGCCTCGGTGTGACCTATCCCGAAATCTACGAAATGCAGGCGC GCGCGATCTTCGAGGCGGCGTGCGACGTCGCCGCCGAAACCGGCGCGGCGCCGGTCCCCGAAGTGATGATCCCGCTGGTC GCGACGCGCCGCGAGTTCGACCTGATGAAGGCGGTCGTGGATGCGCAGGCAAAAGCAGTGTTCGCCGAAAAGGGCCGCGA GATCGCCTATCTTGTCGGTACGATGATCGAACTGCCGCGCGCCGCGCTGATGGCGGGCGAAATTGCCGAGACGGCTGAGT TTTTCAGTTTCGGGACCAACGACCTGACGCAAACGACGATCGGCATCAGCCGCGACGACGCGGGGCGTTTCCTGACCCAA TATGTCGACAAGGGCATCTTCCTGACCGATCCCTTCGTGAGCCTCGATGTCGAGGGCGTCGGACAGCTGATCGAGATCGC CGCCGAGCGCGGCCGGGCGACGCGCGCCGACATCAAACTGGGCATCTGCGGCGAGCATGGCGGCGACGCGCCGAGCATCC ATTTCTGCGAAAAAACCGGCCTCGATTATGTCAGCGCCTCACCCTACCGCGTTCCCATAGCCCGGCTCGCCGCGGCGCAG GCGGCGCTGCGCAAATAA
Upstream 100 bases:
>100_bases GTAGGGAAGTCTGGAAAGACCAAAGCAAAACTGAATCGACCAAGTGCGATTGCATACCTATCCAGCCGCAACCTCCTCCC CGGGGCAGCAGGAGTAAGAC
Downstream 100 bases:
>100_bases TCGCCCAAAGGGGCTTGCGCGACTCCGCACAGGCCCCTAAAGGCACCGCTCCACGCACCCGTAGCTCAGCTGGATAGAGC ATCAGACTACGAATCTGAGG
Product: pyruvate phosphate dikinase
Products: NA
Alternate protein names: Pyruvate, orthophosphate dikinase [H]
Number of amino acids: Translated: 885; Mature: 884
Protein sequence:
>885_residues MTKMVHLFGGAVTTAERSKELLGGKGSNLAEMASIGLPVPPGFTITTDVCTAYYANGEQFPAGLVEEVAAGIAHIEGITG KRFGDAVDPLLVSVRSGARVSMPGMMDTVLNLGLNDKTVVGLAEASGDPRFAWDSYRRFVQMYADVVMGLDHAEFEEALE IAKEDKGFYLDTEMAAEDWQALVKQYQAIVERETGAPFPQEPKDQLWGAVGAVFASWESDRAKVYRRLNSIPAEWGTAVN VQAMVFGNMGDTSATGVAFTRDPATGERAWYGEWLINAQGEDVVAGIRTPQYLTKAARERAGARPLSMEEAMPETFAELG RVFDTLETHYRDMQDIEFTVERGKLWMLQTRSGKRTAKAALKIAVDMAAEGLITEEEAVGRVDPGALDQLLHPTLDPKAP RDVLTKGLPASPGAASGKIMFDADSAEKAAAMGEAVILVRVETSPEDIHGMHAAKGILTARGGMTSHAAVVARGMGRPCV SGAGGLSIDGAARVLRVGGRELREGDILTLDGSTGEVMAGEVPTLMPELVGDFGTLMGWADKVRRMKVRTNAETPQDARV ARDFGAEGIGLCRTEHMFFDAARITAVREMILADSEEGRRAALAKLLPEQRGDFAAIFGVMAGLPVTIRLLDPPLHEFLP TREEDFADVAAAAGVGIEALKARANELHEFNPMLGHRGCRLGVTYPEIYEMQARAIFEAACDVAAETGAAPVPEVMIPLV ATRREFDLMKAVVDAQAKAVFAEKGREIAYLVGTMIELPRAALMAGEIAETAEFFSFGTNDLTQTTIGISRDDAGRFLTQ YVDKGIFLTDPFVSLDVEGVGQLIEIAAERGRATRADIKLGICGEHGGDAPSIHFCEKTGLDYVSASPYRVPIARLAAAQ AALRK
Sequences:
>Translated_885_residues MTKMVHLFGGAVTTAERSKELLGGKGSNLAEMASIGLPVPPGFTITTDVCTAYYANGEQFPAGLVEEVAAGIAHIEGITG KRFGDAVDPLLVSVRSGARVSMPGMMDTVLNLGLNDKTVVGLAEASGDPRFAWDSYRRFVQMYADVVMGLDHAEFEEALE IAKEDKGFYLDTEMAAEDWQALVKQYQAIVERETGAPFPQEPKDQLWGAVGAVFASWESDRAKVYRRLNSIPAEWGTAVN VQAMVFGNMGDTSATGVAFTRDPATGERAWYGEWLINAQGEDVVAGIRTPQYLTKAARERAGARPLSMEEAMPETFAELG RVFDTLETHYRDMQDIEFTVERGKLWMLQTRSGKRTAKAALKIAVDMAAEGLITEEEAVGRVDPGALDQLLHPTLDPKAP RDVLTKGLPASPGAASGKIMFDADSAEKAAAMGEAVILVRVETSPEDIHGMHAAKGILTARGGMTSHAAVVARGMGRPCV SGAGGLSIDGAARVLRVGGRELREGDILTLDGSTGEVMAGEVPTLMPELVGDFGTLMGWADKVRRMKVRTNAETPQDARV ARDFGAEGIGLCRTEHMFFDAARITAVREMILADSEEGRRAALAKLLPEQRGDFAAIFGVMAGLPVTIRLLDPPLHEFLP TREEDFADVAAAAGVGIEALKARANELHEFNPMLGHRGCRLGVTYPEIYEMQARAIFEAACDVAAETGAAPVPEVMIPLV ATRREFDLMKAVVDAQAKAVFAEKGREIAYLVGTMIELPRAALMAGEIAETAEFFSFGTNDLTQTTIGISRDDAGRFLTQ YVDKGIFLTDPFVSLDVEGVGQLIEIAAERGRATRADIKLGICGEHGGDAPSIHFCEKTGLDYVSASPYRVPIARLAAAQ AALRK >Mature_884_residues TKMVHLFGGAVTTAERSKELLGGKGSNLAEMASIGLPVPPGFTITTDVCTAYYANGEQFPAGLVEEVAAGIAHIEGITGK RFGDAVDPLLVSVRSGARVSMPGMMDTVLNLGLNDKTVVGLAEASGDPRFAWDSYRRFVQMYADVVMGLDHAEFEEALEI AKEDKGFYLDTEMAAEDWQALVKQYQAIVERETGAPFPQEPKDQLWGAVGAVFASWESDRAKVYRRLNSIPAEWGTAVNV QAMVFGNMGDTSATGVAFTRDPATGERAWYGEWLINAQGEDVVAGIRTPQYLTKAARERAGARPLSMEEAMPETFAELGR VFDTLETHYRDMQDIEFTVERGKLWMLQTRSGKRTAKAALKIAVDMAAEGLITEEEAVGRVDPGALDQLLHPTLDPKAPR DVLTKGLPASPGAASGKIMFDADSAEKAAAMGEAVILVRVETSPEDIHGMHAAKGILTARGGMTSHAAVVARGMGRPCVS GAGGLSIDGAARVLRVGGRELREGDILTLDGSTGEVMAGEVPTLMPELVGDFGTLMGWADKVRRMKVRTNAETPQDARVA RDFGAEGIGLCRTEHMFFDAARITAVREMILADSEEGRRAALAKLLPEQRGDFAAIFGVMAGLPVTIRLLDPPLHEFLPT REEDFADVAAAAGVGIEALKARANELHEFNPMLGHRGCRLGVTYPEIYEMQARAIFEAACDVAAETGAAPVPEVMIPLVA TRREFDLMKAVVDAQAKAVFAEKGREIAYLVGTMIELPRAALMAGEIAETAEFFSFGTNDLTQTTIGISRDDAGRFLTQY VDKGIFLTDPFVSLDVEGVGQLIEIAAERGRATRADIKLGICGEHGGDAPSIHFCEKTGLDYVSASPYRVPIARLAAAQA ALRK
Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]
COG id: COG0574
COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the PEP-utilizing enzyme family [H]
Homologues:
Organism=Escherichia coli, GI1788756, Length=465, Percent_Identity=26.0215053763441, Blast_Score=98, Evalue=2e-21, Organism=Escherichia coli, GI1789193, Length=471, Percent_Identity=25.6900212314225, Blast_Score=94, Evalue=4e-20, Organism=Escherichia coli, GI1787994, Length=742, Percent_Identity=25.6064690026954, Blast_Score=90, Evalue=7e-19, Organism=Escherichia coli, GI1788726, Length=479, Percent_Identity=23.7995824634656, Blast_Score=81, Evalue=3e-16, Organism=Escherichia coli, GI48994992, Length=456, Percent_Identity=22.8070175438596, Blast_Score=77, Evalue=4e-15,
Paralogues:
None
Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013815 - InterPro: IPR013816 - InterPro: IPR008279 - InterPro: IPR018274 - InterPro: IPR023151 - InterPro: IPR000121 - InterPro: IPR002192 - InterPro: IPR010121 - InterPro: IPR015813 [H]
Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]
EC number: =2.7.9.1 [H]
Molecular weight: Translated: 95049; Mature: 94917
Theoretical pI: Translated: 4.72; Mature: 4.72
Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 3.8 %Met (Translated Protein) 4.6 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 3.7 %Met (Mature Protein) 4.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTKMVHLFGGAVTTAERSKELLGGKGSNLAEMASIGLPVPPGFTITTDVCTAYYANGEQF CCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHCCCCCC PAGLVEEVAAGIAHIEGITGKRFGDAVDPLLVSVRSGARVSMPGMMDTVLNLGLNDKTVV CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHCCCCCCEEE GLAEASGDPRFAWDSYRRFVQMYADVVMGLDHAEFEEALEIAKEDKGFYLDTEMAAEDWQ EEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHH ALVKQYQAIVERETGAPFPQEPKDQLWGAVGAVFASWESDRAKVYRRLNSIPAEWGTAVN HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHCCCEEE VQAMVFGNMGDTSATGVAFTRDPATGERAWYGEWLINAQGEDVVAGIRTPQYLTKAARER EEEEEEECCCCCCCCCEEEECCCCCCCCCEECCEEECCCCCCEEEECCCHHHHHHHHHHH AGARPLSMEEAMPETFAELGRVFDTLETHYRDMQDIEFTVERGKLWMLQTRSGKRTAKAA CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCEEEEEECCCCHHHHHH LKIAVDMAAEGLITEEEAVGRVDPGALDQLLHPTLDPKAPRDVLTKGLPASPGAASGKIM HHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCEEE FDADSAEKAAAMGEAVILVRVETSPEDIHGMHAAKGILTARGGMTSHAAVVARGMGRPCV EECCCHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCCCC SGAGGLSIDGAARVLRVGGRELREGDILTLDGSTGEVMAGEVPTLMPELVGDFGTLMGWA CCCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHHH DKVRRMKVRTNAETPQDARVARDFGAEGIGLCRTEHMFFDAARITAVREMILADSEEGRR HHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCHH AALAKLLPEQRGDFAAIFGVMAGLPVTIRLLDPPLHEFLPTREEDFADVAAAAGVGIEAL HHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHHCCCHHHH KARANELHEFNPMLGHRGCRLGVTYPEIYEMQARAIFEAACDVAAETGAAPVPEVMIPLV HHHHHHHHHCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH ATRREFDLMKAVVDAQAKAVFAEKGREIAYLVGTMIELPRAALMAGEIAETAEFFSFGTN HHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC DLTQTTIGISRDDAGRFLTQYVDKGIFLTDPFVSLDVEGVGQLIEIAAERGRATRADIKL CHHHHEECCCCCHHHHHHHHHHHCCCEEECCCCEEEHHHHHHHHHHHHHCCCCEECCEEE GICGEHGGDAPSIHFCEKTGLDYVSASPYRVPIARLAAAQAALRK EEECCCCCCCCCEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHCC >Mature Secondary Structure TKMVHLFGGAVTTAERSKELLGGKGSNLAEMASIGLPVPPGFTITTDVCTAYYANGEQF CCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHCCCCCC PAGLVEEVAAGIAHIEGITGKRFGDAVDPLLVSVRSGARVSMPGMMDTVLNLGLNDKTVV CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHCCCCCCEEE GLAEASGDPRFAWDSYRRFVQMYADVVMGLDHAEFEEALEIAKEDKGFYLDTEMAAEDWQ EEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHH ALVKQYQAIVERETGAPFPQEPKDQLWGAVGAVFASWESDRAKVYRRLNSIPAEWGTAVN HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHCCCEEE VQAMVFGNMGDTSATGVAFTRDPATGERAWYGEWLINAQGEDVVAGIRTPQYLTKAARER EEEEEEECCCCCCCCCEEEECCCCCCCCCEECCEEECCCCCCEEEECCCHHHHHHHHHHH AGARPLSMEEAMPETFAELGRVFDTLETHYRDMQDIEFTVERGKLWMLQTRSGKRTAKAA CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCEEEEEECCCCHHHHHH LKIAVDMAAEGLITEEEAVGRVDPGALDQLLHPTLDPKAPRDVLTKGLPASPGAASGKIM HHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCEEE FDADSAEKAAAMGEAVILVRVETSPEDIHGMHAAKGILTARGGMTSHAAVVARGMGRPCV EECCCHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCCCC SGAGGLSIDGAARVLRVGGRELREGDILTLDGSTGEVMAGEVPTLMPELVGDFGTLMGWA CCCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHHH DKVRRMKVRTNAETPQDARVARDFGAEGIGLCRTEHMFFDAARITAVREMILADSEEGRR HHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCHH AALAKLLPEQRGDFAAIFGVMAGLPVTIRLLDPPLHEFLPTREEDFADVAAAAGVGIEAL HHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHHCCCHHHH KARANELHEFNPMLGHRGCRLGVTYPEIYEMQARAIFEAACDVAAETGAAPVPEVMIPLV HHHHHHHHHCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH ATRREFDLMKAVVDAQAKAVFAEKGREIAYLVGTMIELPRAALMAGEIAETAEFFSFGTN HHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC DLTQTTIGISRDDAGRFLTQYVDKGIFLTDPFVSLDVEGVGQLIEIAAERGRATRADIKL CHHHHEECCCCCHHHHHHHHHHHCCCEEECCCCEEEHHHHHHHHHHHHHCCCCEECCEEE GICGEHGGDAPSIHFCEKTGLDYVSASPYRVPIARLAAAQAALRK EEECCCCCCCCCEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11481430; 9168612 [H]