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The map label for this gene is ycjG [H]

Identifier: 103486949

GI number: 103486949

Start: 1521021

End: 1522028

Strand: Reverse

Name: ycjG [H]

Synonym: Sala_1464

Alternate gene names: 103486949

Gene position: 1522028-1521021 (Counterclockwise)

Preceding gene: 103486950

Following gene: 103486948

Centisome position: 45.5

GC content: 69.35

Gene sequence:

>1008_bases
ATGGCGATCCGGCGCAGCAGCGAATTGTCGCTCGACCTCCGGGTCGAGCGCTTTCCCTACCACCAGCCCTTCCGCATTTC
CGGCCATGTCTTCACCGAAACCGCGCTGCTCGTCGCCGAACTCTCCGACGGCGAACATGTCGGGCGCGGCGAGGGGGCGG
GCGTCTATTATCTCGGCGACGACATCGAGCATATGCTTGGCCAGGCGACCAGCGTGCGGGGTGCGATCGAGCGCGGCGCG
ACGCGCGAGGATTTGCAGCATCTCCTGCCGCCGGGCGGCGCACGCAACGCGCTCGACTGCGCCTTCTGGGAGCTCGAAGC
CCGGCAGACGGGGCAGCCGGTGTGGCGGCTTGCCGGGCTCGACGCGCCCAGGGCGCTGCGTTCGACGCTGACGCTGGGCG
CCGATACGCCCGAGCGAATGGCCGCGGCGGCGCAGGCGATCGACGTGCAAGCGCCGGTCAAGGTCAAGCTCACCGGCGAC
CTGGCCGACGACGTCGCGCGCATCGAGGCGATCCGCGCCGCGCGCCCCGACGCCTGGATCGGCGTCGACGCCAATCAGGG
CTATGATGTCGGAACGCTGCGCGACCTGCTGCCCGTGCTGCTCGCGGCCGGGGTCGCGCAGCTCGAACAGCCGCTGGGGC
GCGGGTGCGAGGCCGATCTCGACGGGCTGAAGCGTCCCTTGCCCTTCGTCGCCGACGAAAGCGCACTGTCGCTCGCCGAC
ACCGCCTCGCTCGTCGGGCGCTTCGATATGGTGAACATCAAGCTCGATAAATGCGGCGGGTTGACCGAGGGGCTTGCGAT
TGCGCGGCAGGCAAAGACGCTCGGCCTCGACGTGATGGTCGGCAACATGATGGGGACCAGCCTGTCGATGGCGCCATCCT
GTCTGGTCGGGCAGCTGTGCGACATCGTCGACCTCGACGGGCCGACCTTCCTCGCGCGCGACCGGGTGCCGGGGGTGCAG
TATCGCGACGGGATGATTCATTGTCCGGCTGAGGTTTGGGGCGGCTAG

Upstream 100 bases:

>100_bases
GGACAATGAAGCTCGGCGCGGTGCTGCTGATCCTCGCCGCCGCGGTGCTTGTCTGGGTGACACTCGCCTTTCATCTCTAT
GGCTTCGGGATGGTCTATTG

Downstream 100 bases:

>100_bases
CCGTTCTACCGTCCCAATGGTCCCCCCGGCGTGACGAGCCAAATAAGTCAATTTGATGTCCTAAATGGGTTGACAATTTT
CTGATTAGGACAATAGTGAC

Product: mandelate racemase/muconate lactonizing enzyme

Products: 3-chloro-cis,cis-muconate

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAIRRSSELSLDLRVERFPYHQPFRISGHVFTETALLVAELSDGEHVGRGEGAGVYYLGDDIEHMLGQATSVRGAIERGA
TREDLQHLLPPGGARNALDCAFWELEARQTGQPVWRLAGLDAPRALRSTLTLGADTPERMAAAAQAIDVQAPVKVKLTGD
LADDVARIEAIRAARPDAWIGVDANQGYDVGTLRDLLPVLLAAGVAQLEQPLGRGCEADLDGLKRPLPFVADESALSLAD
TASLVGRFDMVNIKLDKCGGLTEGLAIARQAKTLGLDVMVGNMMGTSLSMAPSCLVGQLCDIVDLDGPTFLARDRVPGVQ
YRDGMIHCPAEVWGG

Sequences:

>Translated_335_residues
MAIRRSSELSLDLRVERFPYHQPFRISGHVFTETALLVAELSDGEHVGRGEGAGVYYLGDDIEHMLGQATSVRGAIERGA
TREDLQHLLPPGGARNALDCAFWELEARQTGQPVWRLAGLDAPRALRSTLTLGADTPERMAAAAQAIDVQAPVKVKLTGD
LADDVARIEAIRAARPDAWIGVDANQGYDVGTLRDLLPVLLAAGVAQLEQPLGRGCEADLDGLKRPLPFVADESALSLAD
TASLVGRFDMVNIKLDKCGGLTEGLAIARQAKTLGLDVMVGNMMGTSLSMAPSCLVGQLCDIVDLDGPTFLARDRVPGVQ
YRDGMIHCPAEVWGG
>Mature_334_residues
AIRRSSELSLDLRVERFPYHQPFRISGHVFTETALLVAELSDGEHVGRGEGAGVYYLGDDIEHMLGQATSVRGAIERGAT
REDLQHLLPPGGARNALDCAFWELEARQTGQPVWRLAGLDAPRALRSTLTLGADTPERMAAAAQAIDVQAPVKVKLTGDL
ADDVARIEAIRAARPDAWIGVDANQGYDVGTLRDLLPVLLAAGVAQLEQPLGRGCEADLDGLKRPLPFVADESALSLADT
ASLVGRFDMVNIKLDKCGGLTEGLAIARQAKTLGLDVMVGNMMGTSLSMAPSCLVGQLCDIVDLDGPTFLARDRVPGVQY
RDGMIHCPAEVWGG

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=314, Percent_Identity=39.4904458598726, Blast_Score=193, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 5.5.1.7

Molecular weight: Translated: 35771; Mature: 35640

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRRSSELSLDLRVERFPYHQPFRISGHVFTETALLVAELSDGEHVGRGEGAGVYYLGD
CCCCCCCCCEEEEEEEECCCCCCEEECCEEEHHHEEEEEECCCCCCCCCCCCCEEEEECC
DIEHMLGQATSVRGAIERGATREDLQHLLPPGGARNALDCAFWELEARQTGQPVWRLAGL
HHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHEEEHHHCCCCCCEEECCC
DAPRALRSTLTLGADTPERMAAAAQAIDVQAPVKVKLTGDLADDVARIEAIRAARPDAWI
CCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEE
GVDANQGYDVGTLRDLLPVLLAAGVAQLEQPLGRGCEADLDGLKRPLPFVADESALSLAD
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHHHHH
TASLVGRFDMVNIKLDKCGGLTEGLAIARQAKTLGLDVMVGNMMGTSLSMAPSCLVGQLC
HHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHHHCEEEEECCHHCCCHHHCHHHHHHHHH
DIVDLDGPTFLARDRVPGVQYRDGMIHCPAEVWGG
HHEECCCCCEEECCCCCCCEECCCEEECCHHHCCC
>Mature Secondary Structure 
AIRRSSELSLDLRVERFPYHQPFRISGHVFTETALLVAELSDGEHVGRGEGAGVYYLGD
CCCCCCCCEEEEEEEECCCCCCEEECCEEEHHHEEEEEECCCCCCCCCCCCCEEEEECC
DIEHMLGQATSVRGAIERGATREDLQHLLPPGGARNALDCAFWELEARQTGQPVWRLAGL
HHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHEEEHHHCCCCCCEEECCC
DAPRALRSTLTLGADTPERMAAAAQAIDVQAPVKVKLTGDLADDVARIEAIRAARPDAWI
CCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEE
GVDANQGYDVGTLRDLLPVLLAAGVAQLEQPLGRGCEADLDGLKRPLPFVADESALSLAD
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHHHHH
TASLVGRFDMVNIKLDKCGGLTEGLAIARQAKTLGLDVMVGNMMGTSLSMAPSCLVGQLC
HHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHHHCEEEEECCHHCCCHHHCHHHHHHHHH
DIVDLDGPTFLARDRVPGVQYRDGMIHCPAEVWGG
HHEECCCCCEEECCCCCCCEECCCEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-chloro-2,5-dihydro-5-oxofuran-2-acetate

Specific reaction: 2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]