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The map label for this gene is pbpG [H]

Identifier: 103486938

GI number: 103486938

Start: 1502354

End: 1504453

Strand: Reverse

Name: pbpG [H]

Synonym: Sala_1453

Alternate gene names: 103486938

Gene position: 1504453-1502354 (Counterclockwise)

Preceding gene: 103486939

Following gene: 103486937

Centisome position: 44.97

GC content: 68.0

Gene sequence:

>2100_bases
ATGCTGTGTTGGGGTTGTAATACGGCCTCAAGCATGACCTGGTTCCGCAAACCCGAAGGCTCCGATCCCACCCTTGCGAT
GACGCCGGGCGGGCCGTTCGCCGACGTTCCGCCGTCCCGGTCGCTGCCGCCGTCGTCGCCGCTTCCTGAATCGCGGCGGC
GCAAATGGGTGCGCCGCGTCGCGCGCGGCTTTGCGGTTCTGTGCGTGATTTTCATCCTGCTCGTCGGCTGGCTCGCGCTC
ACCGCGCCGCTGTCGAAATCGCTCGAACCGATCGCGCCGCCGCAGATTACCCTGCTCGCATCCGACGGAACGCCGATCGC
GCGGTCGGGGGCGATCGTCGACCGGCCGGTCAAGACGCGCGACCTGCCGGCTCATGTCACGGGCGCCTTTCTGGCGATCG
AGGACCGGCGTTTCTATTCGCACTGGGGTATCGACCCGCGCAGCATCGCGCGCGCGGTGTGGAGCAACATCACGGGCGGC
CCGACGCAGGGCGGCAGCACGATCACCCAGCAGCTTGCGAAGTTCACCTTTCTGACGCCGAAGCGCACGCTGGGGCGCAA
GGCGCGCGAGGCGCTGATCGCTTTCTGGCTGGAGGCATGGCTGACGAAGGACGAGATACTCGAACGCTATCTGTCGAACG
CCTATTTCGGCGACAATACCTATGGGCTGCGCGCTGCGTCGCTGCATTATTTCTATCGCCAGCCCGAAAACCTGACCCCG
GCGCAGGCGGCGATGCTCGCGGGGCTGGTACAGGCGCCGTCGCGTCTTGCGCCGACGCGCAATCCCGACCTCGCCGAAAA
GCGGATGAAGCTGGTGTTGAAGGCGATGGTCGCGACAGGCCGACTGACCGAGCGACAGGCGCGCGCGATCCGCACCCCGC
GCATCGATGTGCGCACGCGCAACACCTTGCCCACGGGCACCTATTTCGCCGACTGGGCGCTGCCCGAGGCGCGCAAATTG
AGCGACGTCGGCTATTCGCGCCAGACGATCGCGACGACGCTCGACGCGCGGCTCCAGTCGATCGCGCGGCGCGTCACCAG
CCGCGCGGGGCTTGGCAAGGCGCAGGTCGCGCTCGTCGCGATGCGCCCGAATGGCGAGGTCGTCGCGATGATCGGCGGCA
AGGATTATGCCGCCTCGCCCTTCAACCGCGCGACGCAGGCGCGGCGTCAGCCGGGATCGACCTTCAAGCTGTTCGTCTAT
CTCGCCGCGCTGCGCGCCGGGTGGGAGCCCGATGACAAGATCGACAACGGCCCGATCGAAACCGGCGCCTATCGCCCCAG
GAACGCGGGCGGCGCCTATTCGCCGCGGATCAGCCTGGAGGACGCCTTTGCGTCGTCGAGCAATGTCGCGGCGGTGCGCC
TGTTCGCCGAAGTCGGCGGCGACAAGGTGATCGACATGGCGCGCGACCTGGGCGTTACCGCGCCGCTCGCGGAGGGGGAT
CCCAGCCTGGCGCTCGGCACGTCCAGCATGACCCTCCTTGAACTGACGGCGGCCTATGCCGCGGTCGCGGCGAACCGCTA
TCCGGTGAAGCCCCATGCGTTCAGCGAGGAGGAAAAGGGCTGGCTCGAAAATCTCGTATGGGGCCCGTCGCGGTTCAGCT
CGCGCGTGCATCGCGATATGGAAAAGATGCTGCGCGCCGCCGTCAATCGCGGTACCGGGCGCGCCGCGCGGCTGCCGCAG
CCCAATTTCGGCAAGACGGGCACCAGCCAGGATAATCGCGACGCGCTGTTCGTCGGCTATGCCAATGGTCTCGTCGTCGG
CGTGTGGATCGGCAATGACGACAACAGCCCGCTTCGGGGCATTTCGGGCGGCGGCCTGCCCGCGCGCATGTGGCGCGACT
TCATGAGGCAGGCATCGGGGCAAAAGGCTGCGCCGCGACCGCGCCCCGTCGATCCGACCGGCCCCGTCGAGCCGCAGGAT
ACACCGACCATTCCCGATATCGGCGACATCCCCGTCGGCGACCGGACACGCGTGGGCATTGAAGGTGGCGACGCGGTAAT
CTCGACCGAAATCAATGGCAGCCGGATCGACCTTCGCCTGCCGATCGAGCAGCGCCCGCCCGAACCGACCCGGCCCGATG
CAGGCGCGCCGCCGCCCTAG

Upstream 100 bases:

>100_bases
ACTGGGCCTTTCCAACGCTCAGGCCACGGCGTTACAGCGTGTCGCGCGCGATACGCTGCGAAGCGCGGGCAGCTGACGCG
CGCCGACAAGCCATTTGCCT

Downstream 100 bases:

>100_bases
AGCGCGATGACCTGATATTGACCCACCGTGATTGCCCCCTGAAGTTCGTCGGCAAGAAGCGTCTCGCAGGACGGGCTGGT
TGCCCGGACCAGAGACGCGC

Product: penicillin-binding protein 1A

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MLCWGCNTASSMTWFRKPEGSDPTLAMTPGGPFADVPPSRSLPPSSPLPESRRRKWVRRVARGFAVLCVIFILLVGWLAL
TAPLSKSLEPIAPPQITLLASDGTPIARSGAIVDRPVKTRDLPAHVTGAFLAIEDRRFYSHWGIDPRSIARAVWSNITGG
PTQGGSTITQQLAKFTFLTPKRTLGRKAREALIAFWLEAWLTKDEILERYLSNAYFGDNTYGLRAASLHYFYRQPENLTP
AQAAMLAGLVQAPSRLAPTRNPDLAEKRMKLVLKAMVATGRLTERQARAIRTPRIDVRTRNTLPTGTYFADWALPEARKL
SDVGYSRQTIATTLDARLQSIARRVTSRAGLGKAQVALVAMRPNGEVVAMIGGKDYAASPFNRATQARRQPGSTFKLFVY
LAALRAGWEPDDKIDNGPIETGAYRPRNAGGAYSPRISLEDAFASSSNVAAVRLFAEVGGDKVIDMARDLGVTAPLAEGD
PSLALGTSSMTLLELTAAYAAVAANRYPVKPHAFSEEEKGWLENLVWGPSRFSSRVHRDMEKMLRAAVNRGTGRAARLPQ
PNFGKTGTSQDNRDALFVGYANGLVVGVWIGNDDNSPLRGISGGGLPARMWRDFMRQASGQKAAPRPRPVDPTGPVEPQD
TPTIPDIGDIPVGDRTRVGIEGGDAVISTEINGSRIDLRLPIEQRPPEPTRPDAGAPPP

Sequences:

>Translated_699_residues
MLCWGCNTASSMTWFRKPEGSDPTLAMTPGGPFADVPPSRSLPPSSPLPESRRRKWVRRVARGFAVLCVIFILLVGWLAL
TAPLSKSLEPIAPPQITLLASDGTPIARSGAIVDRPVKTRDLPAHVTGAFLAIEDRRFYSHWGIDPRSIARAVWSNITGG
PTQGGSTITQQLAKFTFLTPKRTLGRKAREALIAFWLEAWLTKDEILERYLSNAYFGDNTYGLRAASLHYFYRQPENLTP
AQAAMLAGLVQAPSRLAPTRNPDLAEKRMKLVLKAMVATGRLTERQARAIRTPRIDVRTRNTLPTGTYFADWALPEARKL
SDVGYSRQTIATTLDARLQSIARRVTSRAGLGKAQVALVAMRPNGEVVAMIGGKDYAASPFNRATQARRQPGSTFKLFVY
LAALRAGWEPDDKIDNGPIETGAYRPRNAGGAYSPRISLEDAFASSSNVAAVRLFAEVGGDKVIDMARDLGVTAPLAEGD
PSLALGTSSMTLLELTAAYAAVAANRYPVKPHAFSEEEKGWLENLVWGPSRFSSRVHRDMEKMLRAAVNRGTGRAARLPQ
PNFGKTGTSQDNRDALFVGYANGLVVGVWIGNDDNSPLRGISGGGLPARMWRDFMRQASGQKAAPRPRPVDPTGPVEPQD
TPTIPDIGDIPVGDRTRVGIEGGDAVISTEINGSRIDLRLPIEQRPPEPTRPDAGAPPP
>Mature_699_residues
MLCWGCNTASSMTWFRKPEGSDPTLAMTPGGPFADVPPSRSLPPSSPLPESRRRKWVRRVARGFAVLCVIFILLVGWLAL
TAPLSKSLEPIAPPQITLLASDGTPIARSGAIVDRPVKTRDLPAHVTGAFLAIEDRRFYSHWGIDPRSIARAVWSNITGG
PTQGGSTITQQLAKFTFLTPKRTLGRKAREALIAFWLEAWLTKDEILERYLSNAYFGDNTYGLRAASLHYFYRQPENLTP
AQAAMLAGLVQAPSRLAPTRNPDLAEKRMKLVLKAMVATGRLTERQARAIRTPRIDVRTRNTLPTGTYFADWALPEARKL
SDVGYSRQTIATTLDARLQSIARRVTSRAGLGKAQVALVAMRPNGEVVAMIGGKDYAASPFNRATQARRQPGSTFKLFVY
LAALRAGWEPDDKIDNGPIETGAYRPRNAGGAYSPRISLEDAFASSSNVAAVRLFAEVGGDKVIDMARDLGVTAPLAEGD
PSLALGTSSMTLLELTAAYAAVAANRYPVKPHAFSEEEKGWLENLVWGPSRFSSRVHRDMEKMLRAAVNRGTGRAARLPQ
PNFGKTGTSQDNRDALFVGYANGLVVGVWIGNDDNSPLRGISGGGLPARMWRDFMRQASGQKAAPRPRPVDPTGPVEPQD
TPTIPDIGDIPVGDRTRVGIEGGDAVISTEINGSRIDLRLPIEQRPPEPTRPDAGAPPP

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1788867, Length=532, Percent_Identity=32.7067669172932, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI1786343, Length=518, Percent_Identity=33.2046332046332, Blast_Score=189, Evalue=7e-49,
Organism=Escherichia coli, GI87082258, Length=250, Percent_Identity=39.2, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1789601, Length=153, Percent_Identity=35.9477124183007, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 75904; Mature: 75904

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCWGCNTASSMTWFRKPEGSDPTLAMTPGGPFADVPPSRSLPPSSPLPESRRRKWVRRV
CEEECCCCCCCCCEEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ARGFAVLCVIFILLVGWLALTAPLSKSLEPIAPPQITLLASDGTPIARSGAIVDRPVKTR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCEECCCCCCC
DLPAHVTGAFLAIEDRRFYSHWGIDPRSIARAVWSNITGGPTQGGSTITQQLAKFTFLTP
CCCCHHCEEEEEEECCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC
KRTLGRKAREALIAFWLEAWLTKDEILERYLSNAYFGDNTYGLRAASLHYFYRQPENLTP
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCH
AQAAMLAGLVQAPSRLAPTRNPDLAEKRMKLVLKAMVATGRLTERQARAIRTPRIDVRTR
HHHHHHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEC
NTLPTGTYFADWALPEARKLSDVGYSRQTIATTLDARLQSIARRVTSRAGLGKAQVALVA
CCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
MRPNGEVVAMIGGKDYAASPFNRATQARRQPGSTFKLFVYLAALRAGWEPDDKIDNGPIE
ECCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
TGAYRPRNAGGAYSPRISLEDAFASSSNVAAVRLFAEVGGDKVIDMARDLGVTAPLAEGD
CCCCCCCCCCCCCCCCEEHHHHHCCCCCCEEEEEEHHHCCCHHHHHHHHCCCCCCCCCCC
PSLALGTSSMTLLELTAAYAAVAANRYPVKPHAFSEEEKGWLENLVWGPSRFSSRVHRDM
CCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
EKMLRAAVNRGTGRAARLPQPNFGKTGTSQDNRDALFVGYANGLVVGVWIGNDDNSPLRG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCC
ISGGGLPARMWRDFMRQASGQKAAPRPRPVDPTGPVEPQDTPTIPDIGDIPVGDRTRVGI
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
EGGDAVISTEINGSRIDLRLPIEQRPPEPTRPDAGAPPP
CCCCEEEEEECCCCEEEEECCHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLCWGCNTASSMTWFRKPEGSDPTLAMTPGGPFADVPPSRSLPPSSPLPESRRRKWVRRV
CEEECCCCCCCCCEEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ARGFAVLCVIFILLVGWLALTAPLSKSLEPIAPPQITLLASDGTPIARSGAIVDRPVKTR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCEECCCCCCC
DLPAHVTGAFLAIEDRRFYSHWGIDPRSIARAVWSNITGGPTQGGSTITQQLAKFTFLTP
CCCCHHCEEEEEEECCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC
KRTLGRKAREALIAFWLEAWLTKDEILERYLSNAYFGDNTYGLRAASLHYFYRQPENLTP
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCH
AQAAMLAGLVQAPSRLAPTRNPDLAEKRMKLVLKAMVATGRLTERQARAIRTPRIDVRTR
HHHHHHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEC
NTLPTGTYFADWALPEARKLSDVGYSRQTIATTLDARLQSIARRVTSRAGLGKAQVALVA
CCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
MRPNGEVVAMIGGKDYAASPFNRATQARRQPGSTFKLFVYLAALRAGWEPDDKIDNGPIE
ECCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
TGAYRPRNAGGAYSPRISLEDAFASSSNVAAVRLFAEVGGDKVIDMARDLGVTAPLAEGD
CCCCCCCCCCCCCCCCEEHHHHHCCCCCCEEEEEEHHHCCCHHHHHHHHCCCCCCCCCCC
PSLALGTSSMTLLELTAAYAAVAANRYPVKPHAFSEEEKGWLENLVWGPSRFSSRVHRDM
CCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
EKMLRAAVNRGTGRAARLPQPNFGKTGTSQDNRDALFVGYANGLVVGVWIGNDDNSPLRG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCC
ISGGGLPARMWRDFMRQASGQKAAPRPRPVDPTGPVEPQDTPTIPDIGDIPVGDRTRVGI
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
EGGDAVISTEINGSRIDLRLPIEQRPPEPTRPDAGAPPP
CCCCEEEEEECCCCEEEEECCHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]